ISSN (Print): 2328-3947

ISSN (Online): 2328-3955

Editor-in-Chief: Hari K. Koul




Granule Associated DNase in T-Lymphocytes from Patients with Inflammatory Disease

1Department of Biochemistry, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russian Federation

2Laboratoire de Biochimie et de Microbiologie, Ecole Normale Supérieure de Natitingou (ENS), Université de Parakou, Parakou-Bénin

3Clinic of allergic diseases of Kazan Research Institute of Epidemiology and Microbiology, Kazan, Russian Federation

American Journal of Biomedical Research. 2016, 4(4), 87-93
doi: 10.12691/ajbr-4-4-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Bera C. Скрипов, Cyrille A. VODOUNON, Irina D. RESHETNIKOVA, Boris B. LEGBA, Zinaida I. ABRAMOVA. Granule Associated DNase in T-Lymphocytes from Patients with Inflammatory Disease. American Journal of Biomedical Research. 2016; 4(4):87-93. doi: 10.12691/ajbr-4-4-2.

Correspondence to: Cyrille  A. VODOUNON, Department of Biochemistry, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russian Federation. Email:


Recent data highlight the undeniable role of programmed cell death type I of lymphocytes in the pathogenesis of certain allergic diseases and autoimmune diseases such as Bronchial Asthma and hemorrhagic rectocolitis. But little data exist on the enzymatic activity of secretory granules associated with lymphocytes of patients suffering from these inflammatory diseases. The aim of the study was to characterize the activity of the DNase in the secretory granules of T lymphocytes isolated from peripheral blood of patients with Bronchial Asthma (n = 20) and Hemorrhagic Rectocolitis (n = 20). Thus, the secretory granules were isolated from lymphocytes by the ultracentrifugation method on percoll density gradient. The detection of the activity of the protein extracts was performed by zymography and electrophoresis method. The results reveal the presence of a protein extract with a molecular weight of 66 kDa both at the level of the granules of the lymphocytes of patients suffering from hemorrhagic rectocolitis considered as a classical autoimmune disease and in the granules of lymphocytes of patients with Bronchial Asthma. The study of physicochemical properties showed an increase in the enzymatic activity of DNase of secretory granules when 1 mM Ca2+ was added to the incubation medium at a pH = 7.5. On the other hand, the addition of 1 mM Zn2 + causes the inhibition of enzyme activity. These results suggest that the enzymatic activity of DNase detected in the granule of lymphocytes of patients with Bronchial Asthma and hemorrhagic rectocolitis occurs not only in the fragmentation of double stranded DNA but could also play a role in the apoptotic process of these same T-lymphocytes. The study of the properties of this DNase in the inflammatory diseases could enable to use this protein as a marker for determining the severity of disease.



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Drug resistant Staphylococcus aureus in Clinical Samples at Kampala International University-teaching Hospital, Bushenyi District, Uganda

1School of Pharmacy, Kampala International University-Western Campus. P.O BOX 71, Bushenyi, Uganda

2Department of Biochemistry, Kampala International University-Western Campus. P.O BOX 71, Bushenyi, Uganda

3Department of Microbiology & Immunology, Kampala International University-Western Campus. P.O BOX 71, Bushenyi, Uganda

4School of Pharmacy and Health Sciences, United States International University – Africa, Nairobi, Kenya

American Journal of Biomedical Research. 2016, 4(4), 94-98
doi: 10.12691/ajbr-4-4-3
Copyright © 2016 Science and Education Publishing

Cite this paper:
Janet Nalwoga, Michael Tirwomwe, Albert Nyanchoka Onchweri, Josephat Nyabayo Maniga, Cyprian Mose Nyaribo, Conrad Ondieki Miruka. Drug resistant Staphylococcus aureus in Clinical Samples at Kampala International University-teaching Hospital, Bushenyi District, Uganda. American Journal of Biomedical Research. 2016; 4(4):94-98. doi: 10.12691/ajbr-4-4-3.

Correspondence to: Conrad  Ondieki Miruka, Department of Biochemistry, Kampala International University-Western Campus. P.O BOX 71, Bushenyi, Uganda. Email:


Background: Staphylococcus aureus that is resistant to methicillin is an important nosocomial pathogen that often causes infections that are hard to treat. This is due to the fact that the pathogen is usually resistant to other commonly used antibiotics. The presence of drug resistant MRSA among patients has previously been documented in various parts of Uganda. However no reports have been documented for Kampala International University-Teaching hospital in western Uganda. This study was therefore carried out to determine the prevalence and antibiotic resistance of MRSA strains in the patients hospitalized in the surgical ward. Methods: Wound swabs were collected from both male and female patients hospitalized in the surgical ward. Samples were then cultured in suitable media. The Staphylococcus aureus colonies that were obtained were tested for resistance to oxacillin to determine the strains that were MRSA. Further antibiotic resistance of the MRSA isolates was determined by the disc diffusion method using various antibiotics. Results: Out of the one hundred and fourteen isolates from the clinical samples, only seventy five isolates were clearly identified as S. aureus with 85.3% coagulase positive and 13.3% coagulase negative. Methicillin resistant staphylococcus aureus was prevalent at a rate of 56.1%. among the MRSA isolates, resistance to ciprofloxacin was observed to be the highest while resistance to ceftriaxone was observed to be the least. Conclusion: The high prevalence of MRSA amongst the surgical ward patients requires proper measures to be taken to prevent further spread of the pathogen. It is recommended that the source of this drug resistant strains of MRSA be determined so as to design appropriate interventions to prevent the future emergence of infections that are hard to treat.



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Cloning Open Reading Frame (ORF) of Rv2430c Mycobacterium tuberculosis Indonesian Isolate in Escherichia coli JM 109

1Department Of Biology, Faculty of Life Sciences, Hasanuddin University, Indonesia

2Department of Microbiology, Faculty of Medicine, Hasanuddin University, Indonesia

3Development Ministry of Health of Republic of Indonesia, Central Basic Biomedical, Health Technology National Institute of Health and Research, Indonesia

American Journal of Biomedical Research. 2016, 4(4), 99-101
doi: 10.12691/ajbr-4-4-4
Copyright © 2016 Science and Education Publishing

Cite this paper:
Rosana Agus, Muhammad Nasrum Massi, Francisca Srioetami Tanoerahardjo. Cloning Open Reading Frame (ORF) of Rv2430c Mycobacterium tuberculosis Indonesian Isolate in Escherichia coli JM 109. American Journal of Biomedical Research. 2016; 4(4):99-101. doi: 10.12691/ajbr-4-4-4.

Correspondence to: Rosana  Agus, Department Of Biology, Faculty of Life Sciences, Hasanuddin University, Indonesia. Email:


Various strategies have been implemented to prevent tuberculosis. Vaccination with Bacille Calmette Guerin (BCG) vaccine is still used around the world. Generally, most people in have gained BCG vaccine as an infant, but the effectiveness of these vaccines do not survive to adulthood. Therefore, the necessary replacement BCG vaccine more effective to eliminate tuberculosis. Mycobacterium tuberculosis is an intracellular pathogen and it was inside the macrophage, which is considered to be the most important component of the immune system. M. tuberculosis has two sets of genes are highly polymorphic referred to as PE and PPE families. These unique families of proteins account for about 10% of the mycobacterial genome and have attracted great interest from a variety of different studies around the world. One member of the as a vaccine candidate is Rv 2430c. It is known that the sera of all patients infected with TB showed strong antibody responses against Rv 2430c compared to healthy individuals. The existence of these antibodies indicates that this protein is found in vivo during infection and is a native immunogenic molecule. The purpose of this study was to clone the Open Reading Frame (ORF) Rv 2430c M. tuberculosis Indonesian isolates to host cells Esherichia coli JM 109. The method used is by isolating -chromosomal DNA from clinical isolates from Indonesia, amplifying the ORF Rv 2430c with , ligating into cloning vectors pGEM-T and transform to E.coli JM 109. Characterization of clones do with migration analysis, restriction analysis and . The results obtained are recombinant clones that carry insertion was Rv 2430cKeywords: Mycobacterium tuberculosis, Rv 2430c, ORF, , JM 109.



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