ISSN (Print): 2328-3947

ISSN (Online): 2328-3955

Editor-in-Chief: Hari K. Koul




Methylenetetrahydrofolate Reductase C677T and Platelet Glycoprotein IIb/IIIa Genes Polymorphism in Myocardial Infarction Egyptian Patients in Ismailia City

1Medical Biochemistry, Faculty of Medicine, Suez Canal University, Ismailia, Egyp

2Cardiology Departments, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

American Journal of Biomedical Research. 2016, 4(3), 74-79
doi: 10.12691/ajbr-4-3-3
Copyright © 2016 Science and Education Publishing

Cite this paper:
Kefah H Ali, Nagwan A Sabek, Loaa A Tag Eldeen, Emad F Ismail, Gamela Nasr. Methylenetetrahydrofolate Reductase C677T and Platelet Glycoprotein IIb/IIIa Genes Polymorphism in Myocardial Infarction Egyptian Patients in Ismailia City. American Journal of Biomedical Research. 2016; 4(3):74-79. doi: 10.12691/ajbr-4-3-3.

Correspondence to: Nagwan  A Sabek, Medical Biochemistry, Faculty of Medicine, Suez Canal University, Ismailia, Egyp. Email:


Background: Hyperhomocysteinemia and platelet glycoprotein GpIIIa polymorphism had been identified as risk factors for coronary atherosclerosis. The methylenetetrahydrofolate reductase MTHFR C677T variant has been shown to influence homocysteine metabolism, the interaction of plasma tHcy with other conventional risk factors remain uncertain in the clinical setting of acute myocardial infarction (AMI). The present study aimed to examine whether the MTHFR and platelet glycoprotein IIIa polymorphisms were associated with increased risk of (MI) in Egyptian patients. Subjects and Method: 150 newly diagnosed MI patients and 50 healthy matched subjects were recruited into this study, genotyping of the MTHFR C677T and GpIIIa 1565 A1/A2 polymorphisms were carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, plasma tHcy, and folic acid levels were estimated. Results: Fasting plasma total Hcy levels were significantly higher in MI patients than controls (P <0.05), folate levels were significantly lower in MI patients than controls (P <0.05), no significant differences were observed in the MTHFR C677T and GpIIIa genotypes frequencies between MI patients and controls (P > 0.05). The frequency of the MTHFR C allele was 80.6 % and 76 % in MI patients and controls respectively and did not differ significantly between the two groups (P > 0.05). The frequency of risk allele, GpIIIa, PIA2 was significantly higher in MI patients compared to controls (p<0.05), plasma tHcy level was significantly higher and folate level was significantly lower in MI patients carrying MTHFR CC and GPIIIa PIA2A2genotypes. Conclusions: In this population, the both risk alleles of MTHFR and GpIIb/IIIa polymorphisms had no major effect on the MI incidence, they were associated with higher homocysteine levels. A gene-environment interaction might increase the risk indirectly by elevating tHcy, especially when folate intake was low, our findings might support that MTHFR and GpIIb/IIIa polymorphisms as risk factors for MI.



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Toxicity Evaluation of the Aqueous Stem Extracts of Senna alata in Wistar Rats

1Department of Biochemistry, Abia State University, Uturu, Abia State, Nigeria

2Federal Medical Centre Umuahia, Abia State, Nigeria

3Department of Biochemistry, Rhema University, Aba, Abia State Nigeria

American Journal of Biomedical Research. 2016, 4(4), 80-86
doi: 10.12691/ajbr-4-4-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
Ugbogu A.E., Okezie E., Uche-Ikonne C., Duru M., Atasie O.C.. Toxicity Evaluation of the Aqueous Stem Extracts of Senna alata in Wistar Rats. American Journal of Biomedical Research. 2016; 4(4):80-86. doi: 10.12691/ajbr-4-4-1.

Correspondence to: Ugbogu  A.E., Department of Biochemistry, Abia State University, Uturu, Abia State, Nigeria. Email:


Aim: This study evaluated the phytochemicals, proximate and the toxicity effect of aqueous stem extract of Senna alata using wistar rats. In acute toxicity test, aqueous dried stem extract of Senna alata were administered orally up to 10 g/kg body weight to male wistar rats. Materials and methods: In sub-acute study, the wistar rats were daily administered orally with aqueous dried stem extract of Senna alata at doses of 250, 500 and 1000 mg/kg for 14 days and haematological, biochemical parameters were determined and a histopathology of the liver and kidney were analyzed. Results: The acute toxicity of oral administration of aqueous extracts of Senna alata stem on albino rats after 24 hours did not produce any mortality at concentration up to 10 g/kg body weight. In the subacute toxicity, significant differences (P> 0.05) were observed in the results of urea, creatinine, and bicarbonate across the tested groups when compared with the control rats. The results of the liver enzymes showed significant difference (P> 0.05) on alkaline transaminase (ALT), Aspartate aminotransferase (AST) and Alkaline phosphatase (ALP). Conclusion: Our results showed that the dried stem of Senna alata is not toxic at the tested doses.



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Granule Associated DNase in T-Lymphocytes from Patients with Inflammatory Disease

1Department of Biochemistry, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russian Federation

2Laboratoire de Biochimie et de Microbiologie, Ecole Normale Supérieure de Natitingou (ENS), Université de Parakou, Parakou-Bénin

3Clinic of allergic diseases of Kazan Research Institute of Epidemiology and Microbiology, Kazan, Russian Federation

American Journal of Biomedical Research. 2016, 4(4), 87-93
doi: 10.12691/ajbr-4-4-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Bera C. Скрипов, Cyrille A. VODOUNON, Irina D. RESHETNIKOVA, Boris B. LEGBA, Zinaida I. ABRAMOVA. Granule Associated DNase in T-Lymphocytes from Patients with Inflammatory Disease. American Journal of Biomedical Research. 2016; 4(4):87-93. doi: 10.12691/ajbr-4-4-2.

Correspondence to: Cyrille  A. VODOUNON, Department of Biochemistry, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russian Federation. Email:


Recent data highlight the undeniable role of programmed cell death type I of lymphocytes in the pathogenesis of certain allergic diseases and autoimmune diseases such as Bronchial Asthma and hemorrhagic rectocolitis. But little data exist on the enzymatic activity of secretory granules associated with lymphocytes of patients suffering from these inflammatory diseases. The aim of the study was to characterize the activity of the DNase in the secretory granules of T lymphocytes isolated from peripheral blood of patients with Bronchial Asthma (n = 20) and Hemorrhagic Rectocolitis (n = 20). Thus, the secretory granules were isolated from lymphocytes by the ultracentrifugation method on percoll density gradient. The detection of the activity of the protein extracts was performed by zymography and electrophoresis method. The results reveal the presence of a protein extract with a molecular weight of 66 kDa both at the level of the granules of the lymphocytes of patients suffering from hemorrhagic rectocolitis considered as a classical autoimmune disease and in the granules of lymphocytes of patients with Bronchial Asthma. The study of physicochemical properties showed an increase in the enzymatic activity of DNase of secretory granules when 1 mM Ca2+ was added to the incubation medium at a pH = 7.5. On the other hand, the addition of 1 mM Zn2 + causes the inhibition of enzyme activity. These results suggest that the enzymatic activity of DNase detected in the granule of lymphocytes of patients with Bronchial Asthma and hemorrhagic rectocolitis occurs not only in the fragmentation of double stranded DNA but could also play a role in the apoptotic process of these same T-lymphocytes. The study of the properties of this DNase in the inflammatory diseases could enable to use this protein as a marker for determining the severity of disease.



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