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American Journal of Biomedical Research is a peer-reviewed, open access journal that provides rapid publication of articles in all areas of biomedical research. The goal of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of biomedical research.

ISSN (Print): 2328-3947

ISSN (Online): 2328-3955

Editor-in-Chief: Hari K. Koul

Website: http://www.sciepub.com/journal/AJBR



Effects of Chronic Alcohol Ingestion on Hematological Parameters in Albino Mice Experimentally Challenged with Escherichia coli Strain 0157:H7

1Department of Animal Production and health, Faculty of Agriculture, Federal University, Oye-ekiti, Ekiti State, Nigeria

2Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P.M.B. 1115 Calabar, Nigeria

3Department of Fisheries and Aqua-culture, Faculty of Agriculture, Federal University, Oye-ekiti, Ekiti State, Nigeria

4Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Abuja, Nigeria

5Department of Veterinary Microbiology and Pathology, University of Nigeria, Nsukka, Enugu State, Nigeria

American Journal of Biomedical Research. 2015, 3(2), 21-28
doi: 10.12691/ajbr-3-2-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Asuzu Onyeka V., Nwaehujor Chinaka O., Okeke Onyinye S., Ode Julius O., Chah Kennedy F.. Effects of Chronic Alcohol Ingestion on Hematological Parameters in Albino Mice Experimentally Challenged with Escherichia coli Strain 0157:H7. American Journal of Biomedical Research. 2015; 3(2):21-28. doi: 10.12691/ajbr-3-2-2.

Correspondence to: Nwaehujor  Chinaka O., Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P.M.B. 1115 Calabar, Nigeria. Email: chinaka_n@yahoo.com


The aim of this study was to investigate the effect of chronic alcohol consumption on hematology when challenged with E. coli strain 0157:H7 using albino mice as experimental model. Eight weeks old mice (26.6 – 35.3 g) of both sexes were used in the study and were divided into 6 groups of 12 mice each using stratified random selection method. Group 1 was given 10 % ethanol (V/V) in their drinking water. Group 2 received 20 % of ethanol. The third group received 30 % of ethanol while Group 4 and 5 received 40 % of alcohol ad-libitum respectively. Group 6 served as control and received only distilled water. The alcohol-treated groups received ethanol for 3 weeks to establish a chronic state of alcoholism and Groups 1-4 were then challenged with E. coli strain 0157:H7 for 7 days. Blood samples were collected via the median canthus of the eyes from the retrobublar plexus. The blood samples were allowed to clot and the sera was obtained by aspiration into Bijou bottles for hematology analysis like packed cell volume (PCV), hemoglobin (Hb), white blood cell (WBC), red blood cell (RBC), neutrophil, lymphocytes, monocytes, eosinophil, and basophil. There was a significance difference (P< 0.05) in the mean WBC, RBC, PCV and Hb values between various groups. The means of WBC of the groups exposed to 10 % alcohol with E. coli (9.2±0.1) had a significantly higher (P<0.05) value than all the other alcohol-treated groups. The mean PCV of groups exposed to 40 % alcohol with E. coli (36.67±0.88), 30 % alcohol with E. coli (37±1.08) and 20 % alcohol with E. coli (37.50±1.19) were significantly (P<0.05) less than that of groups exposed to 10 % alcohol with E. coli (39.60±1.40). Groups that consumed 10% alcohol with E. coli, water with E. coli (41.67±0.88), and water without E. coli (42.25±1.31) were significantly (P<0.05) lower than the group that consumed 40 % alcohol without E. coli challenge (46.25±0.75), but higher than the groups treated with alcohol and challenged with E. coli. There was significant difference (P< 0.05) in the RBC mean values between the various groups. The mean values of groups exposed to 40% alcohol with E. coli (899.2±116.58) and 30 % alcohol with E. coli (923.3±38.37) were significantly (P<0.05) lower than 20 % alcohol with E. coli (978.3±46.39), water with E. coli (985±31.75), water without (998.8±85.81) and 40 % alcohol without E. coli (1068.2±22.58). Neutrophil, lymphocyte and monocyte values across the various groups revealed significant differences (P< 0.05) among the different groups. The results showed that chronic alcohol (ethanol) consumption has adverse pathologic effects on the packed cell volume (PCV), hemoglobin (Hb), white blood cell (WBC), red blood cell (RBC), neutrophil, lymphocytes, monocytes, eosinophil, and basophil. Although alcohol is generally obtained from the fermentation of starch-containing food, its abuse and daily consumption causes damage hematological parameters in the body. Thus, when such a body is challenged with a pathogenic organism, there is less resistance to systemic entry of the cells by the organism, faster access to body cell due to the dehydration effect, and a quick necrotic time due to the toxins produced by such pathogenic organisms.



[1]  Diehl AM (2002) Liver disease in alcohol abusers: Clinical perspective. Alcohol 27:7-11.
[2]  Kril JJ and Halliday GM (1999) Brain shrinkage in alcoholics: A decade in and what have we learned? Prog. Neurobiol., 58:381-387.
[3]  Thomas AP, Rozanski DJ, Renard DC, and Rubin E (1994) Effect of ethanol on the contractile function of the heart: A review. Alcohol. Clin. Exp. Res., 18: 121-131.
[4]  Beckemeier ME, and Bora PS (1998) Fatty acid ethyl esters: Potentially toxic products of myocardial ethanol metabolism. J. Mol. Cell. Cardiol. 30:2487-2494.
[5]  Urbano-Marquez A, Estruch R, Fernandez-Sola J, et al (1995) The greater risk of alcoholic cardiomyopathy and myopathy in women compared with men. JAMA, 274:149-154.
Show More References
[6]  Hansagi H, Romelsjo A, Gerhardsson de Verdier M, et al. (1995) Alcohol consumption and stroke mortality: 20-year follow-up of 15,077 men and women. Stroke, 26:1768-1773.
[7]  Vogt RL, Dippold L (2005) “Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June-July 2002”. Public Health Rep., 120 (2): 174-8.
[8]  Bentley R, Meganathan R (1982) “Biosynthesis of vitamin K (menaquinone) in bacteria”. Microbiol. Rev. 46 (3): 241-80.
[9]  Hudault S, Guignot J, Servin AL (2001) “Escherichia coli strains colonising the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection”. Gut 49 (1): 47-55.
[10]  Reid G, Howard J, Gan BS (September 2001) “Can bacterial interference prevent infection?”. Trends Microbiol. 9 (9): 424-8.
[11]  Feng P, Weagant S, Grant M (2002) “Enumeration of Escherichia coli and the Coliform Bacteria”. Bacteriological Analytical Manual (8th Ed.). FDA/Center for Food Safety & Applied Nutrition. http://www.cfsan.fda.gov/~ebam/bam-4.html. Retrieved 2014-01-25.
[12]  Thompson A (2007) “E. coli Thrives in Beach Sands”. Live Science. http://www.livescience.com/health/070604_beach_ecoli.html. Retrieved 2014-07-03.
[13]  Lawrence JG and Ochman H (1998) Molecular archaeology of the Escherichia coli genome Proc. Natl. Acad. Sci. USA 95:9413-9417.
[14]  Nataro JP, Kaper JB (1998) “Diarrheagenic Escherichia coli”. Clin. Microbiol. Rev., 11 (1): 142-201.
[15]  Ward JW and Elsea JR (1997) Animal case and use in drug fate and metabolism. Methods and techniques, Vol. 1. Ed. Edward R. Garrette and Jean L. Hirtz, Marcel Dekker, New York, pp. 372-390.
[16]  Zimmermann M (1983) Ethical guidelines for investigations of Experimental pain in Conscious Animals. Pain, 16: 109-110.
[17]  Anonymous (1996) Institute of laboratory Animal Resources, commission on life sciences. National Research Council Guide for the care and use of laboratory animals Washington, D.C. National Academy Press p. 46.
[18]  Schlam OW, Jain NC and Carol EI (1975) Veterinary Heamatology. 3rd Ed. Lea and Fibinger, Philadelphia. Pp 144 – 167.
[19]  Gehlbach SH, MacCormack JN, Drake BM, Thompson WV (1973) “Spread of disease by fecal- oral route in day nurseries”. Health Service Reports 88 (4): 320-322.
[20]  Szabo G (1999). Consequences of alcohol consumption on host defense. Alcohol and Alcoholism 34:830-841.
[21]  Addolorato G, Montalto M, Capristo E, et al. (1997) Influence of alcohol on the gastrointestinal motility: Lactulose breathe hydrogen testing in orocecal transit time in chronic alcoholics, social drinkers, and teetotaler subjects. Hepatogastroenterology, 44: 1076-1081.
[22]  Fickert P, and Zatloukal K (2000) Pathogenesis of alcoholic liver disease. In, Handbook of alcoholism. (Zernig G, Saria A, Kurz M, and O’Malley S Ed.) CRC Press, Boca Raton, Fl., pp. 317-323.
[23]  Lieber CS (2000) Alcohol and the liver: Metabolism of alcohol and its role in hepatic and extrahepatic diseases. Mt. Sinai J. Med., 67:84-94.
[24]  Rolhion N, Darfeuille-Michaud A (2007) “Adherent-invasive Escherichia coli in inflammatory bowel disease”. Inflamm. Bowel Dis. 13 (10): 1277-83.
[25]  Baumgart M, Dogan B, Rishniw M et al. (2007) “Culture independent analysis of ileal mucosa reveals a selective increase in invasive Escherichia coli of novel phylogeny relative to depletion of Clostridiales in Crohn's disease involving the ileum”. ISME J 1 (5): 403-418.
[26]  Ridker PM, Vaughan DE, Stampfer MJ, et al. (1994) Association of moderate alcohol consumption and plasma concentration of endogenous tissue-type plasminogen activator. JAMA, 272: 929-933.
[27]  Rimm EB, Williams P, Fosher K, et al. (1999) Moderate alcohol intake and lower risk of coronary heart disease: Meta-analysis of the effects of lipids and haemostatic factors. BMJ, 319:1523-1528.
[28]  Rubin R (1999) Effects of alcohol on platelet function. Alcohol. Clin. Exp. Res., 23:1114-1118.
[29]  Rossinen J, Sinisalo J, Partanen J, et al. (1999) Effects of acute alcohol infusion on duration and dispersion of QT interval in male patients with coronary artery disease and in healthy controls. Clin. Cardiol., 22:591-594.
[30]  Kupari M, and Koskinen P (1998) Alcohol cardiac arrhythmias, and sudden death. In, Alcohol and Cardiovascular diseases. (Goode J., Ed.) Wiley, New York, 61:5-12.
Show Less References


Hepatoprotective, DNA Damage Prevention and Antioxidant Potential of Spirulina platensis on CCl4-Induced Hepatotoxicity in Mice

1Genetics Branch, Botany Department, Faculty of Agriculture, Saba Basha, Alexandria University, Egypt

2Marine Biotechnology and Natural products lab, The National Institute of Oceanography and fisheries, Egypt

3Biophysics and Laser Science Unit, Basic Science Department, Research Institute of Ophthalmology

American Journal of Biomedical Research. 2015, 3(2), 29-34
doi: 10.12691/ajbr-3-2-3
Copyright © 2015 Science and Education Publishing

Cite this paper:
AshganA. AbouGabal, Haiam M. Aboul-Ela, EmanM Ali, Ahemd E.M khaled, Ola Kh. Shalaby. Hepatoprotective, DNA Damage Prevention and Antioxidant Potential of Spirulina platensis on CCl4-Induced Hepatotoxicity in Mice. American Journal of Biomedical Research. 2015; 3(2):29-34. doi: 10.12691/ajbr-3-2-3.

Correspondence to: Haiam  M. Aboul-Ela, Marine Biotechnology and Natural products lab, The National Institute of Oceanography and fisheries, Egypt. Email: haiam_morsy@yahoo.com


In the present study, we have evaluated the hepatoprotective and antioxidant effects of Spirulina platensis against CCl4-induced hepatotoxicity in mice. Activities of liver marker enzymes; Alanine transaminase and Aspartate transaminasewere estimated, as well as lipid peroxidation and antioxidant status (glutathione peroxidase) were determined in liver homogenate. DNA damage in liver was also evaluated by means of Comet assay. CCl4 induction (1 mg/kg b.wt) significantly increases the levels of liver marker enzymesand lipid peroxidation, and caused the depletion of antioxidant status. Treatment of Spirulinaplatensis (800mg/kg/b.wt) to CCl4 challenged mice resulted in decreased liver marker enzymes activity, DNA damage and lipid peroxidation levels with increase in antioxidant status. Our study clearly demonstrates that Spirulina platensis shows hepatoprotective effect through its antioxidant activity on CCl4-induced hepatotoxicity in mice.



[1]  Abd El-Baky HH, El Baz FK, El-Baroty GS (2007) Enhancement of Antioxidant Production in Spirulina platensis under Oxidative Stress. ActaPhysiologiaePlantarum 31: 623-631.
[2]  Abdel-Daim MM, Abuzead SMM, Halawa SM (2013) Protective role of Spirulina platensis against acute deltamethrin-induced toxicity in rats. Plos one 8: e72991..
[3]  Abraham P (2005) Oxidative stress in paracetamol induced pathogenesis: (I). Renal damage. Indian Journal of Biochemistry and Biophysics 42:59-62.
[4]  Alam MA, Haider N, Ahmed S, Alam MT, Aziz A, Perveen A (2013) Tahlab (Spirulina) and few other medicinal plants having anti-oxidant &immunomodulatory properties described in Unani medicine- A review. International Journal of Pharmaceutical Sciences and Research 4: 4158-4164.
[5]  Ames BN, Shigenage MK, Hagen TM. Oxidants, antioxidants and the degenerative diseases of aging. Proceed Nat Acad Sci USA. 1993; 90(17):7915-7922.
Show More References
[6]  Ayehunie S, Belay A, Baba TW, Ruprecht RM (1998) Inhibation of HIV-1 replication by an aqueous extract of Spirulina platensis (Arthrospira platensis). Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology 18: 7-12.
[7]  Bhat VB, Madyastha M (2001) Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: protection against oxidative damage to DNA. Biochemical and Biophysical Research Communications 285: 262-266.
[8]  Bhat, V.B. and Madyastha, K.M. 2000. C-phycocyanin: a potent peroxyl radical scavenger in vivo and in vitro. Biochem. Biophys. Res. Commun, (275): 20-25.
[9]  Chaung, S. S., Lin C. C., Lin, J., Yu, K. H., Hsu, Y. F., and Yen, M. H. (2003) “The hepatoprotective effects of Limoniumsinense against carbon tetrachloride and beta-D-galactosamine intoxication in rats,”Phytotherapy Research, vol. 17, pp. 784-791.
[10]  Deepti G, Shabad P, Dua KK (2011) Prevention of cadmium bioaccumulation by herbal adaptogen: Spirulina platensis. Journal of Chemical and Pharmaceutical Research 3: 603-608.
[11]  Duncan, D. B. (1955). Multiple range and multiple F test. Biometrics.,11: 1-42.
[12]  Ferrira-Hermosillo A,Torres-Duràn PV, Shamosh-Halabe S, Juàrez-Oropeza MA (2011) Biological effects of Spirulina and current research on its antioxidant activity. RevistaInternacional de CienciayTecnologiaBiomédica. Toctli RICTB, 2:1.
[13]  Fridovich I, (1983) Superoxide radical: An endogenous toxicant. Annual Review of Pharmacology and Toxicology 23:239-257.
[14]  Galal RM, Zaki HF, Seif El-Nasr MM, Agha AM (2012) Potential protective effect of honey against paracetamol-induced hepatotoxicity. Archives of Iranian Medicine 15:674-680.
[15]  Gini C. Kuriakose and Muraleedhara G. Kurup, 2010. Hepatoprotective effect of Spirulina lonar on paracetamol induced liver damage in rats. Asian journal biology science. 1(3), 614-623.
[16]  Halliwell B. Why and how should we measure oxidative DNA damage in nutritional studies? How far have we come? Am J ClinNutr. 2000;72(5):1082-1087.
[17]  Hemalatha K, Pugazhendy K, Jayachandran K, Jayanthi C, Meenambal M (2012) Studies on the Protective Efficacy of Spirulina against Lead Acetate Induced Hepatotoxicity in Rattusnorvegicus. International Journal of Chemical and Analytical Science 3: 1509-1512.
[18]  Hurkadale PJ, Shelar PA Palled SG, Mandavkar YD Khedkar, AS (2012) Hepatoprotective activity of Amorphophalluspaeoniifolius tubers against paracetamol-induced liver damage in rats. Asian Pacific Journal of Tropical Biomedicine 2: S238-S242.
[19]  Ibrahim M, Khaja ZU, Narasu ML (2011) Hepatoprotective activity of Boswelliaserrata extracts: in vitro and in vivo studies. International Journal of Pharmaceutical Applications 2:89-98.
[20]  Jackson, A.L. and Loeb, L.A. (2001) The contribution of endogenous sources of DNA damage to the multiple mutations in cancer. Mutation Research 477, 7-21.
[21]  Jarouliya U, Zacharia JA, Kumar P, Bisen PS, Prasad GB (2012) Alleviation of metabolic abnormalities induced by excessive fructose administration in wistar rats by Spirulina maxima. Indian Journal of Medical Research 135: 422-428.
[22]  Kaji, T., Fujiwara, Y. and Inomata, Y. 2002. Repair of wounded monolayers of cultured bovine aortic endothelial cells is inhibited by calcium spirulina, a novel sulfated polysaccharide isolated from Spirulina platensis. Life Sci., 70:1841-1848.
[23]  Khan M, Shobha JC, Mohan IK, Naidu MU, Sundaram C, Singh S, Kuppusamy P, Kutala VK (2005) Protective effect of Spirulina against doxorubicin-induced cardiotoxicity. Phytotherapy Research 19: 1030-1037.
[24]  Lalitsingh Ranawat, Jigar Bhatt, Jagruti Patel. (2010). Hepatoprotective activity of ethanolic extracts of bark of Zanthoxylumarmatum DC in CCl induced hepatic damage in rats. Journal of Ethnopharmacology 127: 777-780.
[25]  Mayuren C, Reddy VV, Priya SV, Devi VA (2010) Protective effect of Livactine against CCL4 and paracetamol induced hepatotoxicity in adult Wister rats. North American Journal of Medical Sciences 2:492-495.
[26]  Murugesh KS, Yeligar VC, Maiti BC and Maity TK (2005). Hepatoprotective and Antioxidant Role of BerberistinctoriaLesch leaves on Paracetamol induced hepatic damage in rats. Iranian Journal of Pharmacology and Therapeutics 4(1) 64-69.
[27]  Nan, P., Xiao X., Yan D.U., Jian C. and Zhong, C. 2013. Genotoxic effects of 8-hydroxylquinoline in loach (Misgurnusanguillicaudatus) assessed by the micronucleus test, comet assay and RAPD analysis. Environmental toxicology and pharmacology, (35): 434-443.
[28]  Nandhakumar S, Parasuraman S, Shanmugam M M, RamachandraRao K, Chand P, Bhat B V. (2011). Evaluation of DNA damage using single-cell gel electrophoresis (Comet Assay). J PharmacolPharmacother; 2:107-11
[29]  Pari L, Suresh A (2008) Effect of grape (Vitisvinifera L.) leaf extract on alcohol induced oxidative stress in rats. Food and Chemical Toxicology 46: 1627-1634.
[30]  Parikh P, Mani U, Iyer U (2001) Role of Spirulina in the control of glycemia and lipidemia in type 2 diabetes Mellitus. Journal of Medicinal Food 4: 193-199.
[31]  Ponce-Canchihuamán JC, Pérez-Méndez O, Hernández-Muñoz R, Torres-Durán PV, Juárez-Oropeza MA (2010) Protective effects of Spirulina maxima on hyperlipidemia and oxidative-stress induced by lead acetate in the liver and kidney. Lipids in Health and Disease 9: 35.
[32]  Premkumar K, Abraham SK, Santhiya ST, Ramesh A (2004) Protective effect of Spirulina fusiformis on chemical induced genotoxicity in mice. Fitoterapia 75: 24-31.
[33]  Price, C.P. and Alberti, K.G.M.M. (1985). Biochemical Assessment of Liver Functions. In:Wright R. Millword-Sadler GH, Alberti KGMM, Karran S. Eds. Liver and Biliary Diseases. ed. London. Pp.455-494.
[34]  Recknagel, R.O., E.A. Jr. Glende, J.A. Dolak and R.L. Waller. (1989). Mechanism of carbon tetrachloride toxicity. Pharmacol. Ther., 43 :139-154.
[35]  Rehab M, Ibrahim M (2012) Evaluation of the effect of Spirulina against gamma irradiation induced oxidative stress and tissue injury in rats. International Journal of Applied Science and Engineering Research 1: 152-164.
[36]  Sabina EP, Samuel J, RajappaRamya S, Patel S, Mandal N, Pranatharthiiharan P, Mishra PP, Rasool M (2009) Hepatoprotective and antioxidant potential of Spirulina fusiformis on acetaminophen-induced hepatotoxicity in mice. International Journal of Integrative Biology 6: 1-5.
[37]  Sallie, R., Tredger, J.M., Willam, R., 1991. Drugs and the liver. Biopharm. Drug Dispos. 12, 251-259.
[38]  SAS (2001). SAS User’s Guide Statistic. SAS Version 8.2. Inc. Cary. NC. USA.
[39]  Saxena PS, Kumar M (2004) Modulatory potential of Spirulina fusiformis on testicular phosphatases in Swiss albino mice against mercury intoxication. Indian Journal of Experimental Biology 42: 998-1002.
[40]  Sharma, M.K., Sharma, A., Kumar, A. and Kumar, M. 2007. Evaluation of protective efficacy of Spirulina against mercury induced nephrotoxicity in Swiss albino mice. Food and Chemical Toxicology, (45): 879-887.
Show Less References


Evaluation of Thrombolytic and Cytotoxic activities of an Ornamental medicinal plant: Byttneria pilosa

1Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh

2Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka-1229, Bangladesh

3State University of Bangladesh, 138, Mirpur Road, Dhaka-1205, Bangladesh

American Journal of Biomedical Research. 2015, 3(3), 35-39
doi: 10.12691/ajbr-3-3-1
Copyright © 2015 Science and Education Publishing

Cite this paper:
Mubarik Yusuf Ibrahim, Prawej Ansari, AKM Riasat-ul-Islam, Mahmuda Sultana, Nadia Akter Zhumur, Shah Mohammed Shafi. Evaluation of Thrombolytic and Cytotoxic activities of an Ornamental medicinal plant: Byttneria pilosa. American Journal of Biomedical Research. 2015; 3(3):35-39. doi: 10.12691/ajbr-3-3-1.

Correspondence to: Prawej  Ansari, Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh. Email: chemist89ansari@gmail.com


Purpose: The rapidly growing incidence of ischemic stroke caused by thrombosis of the arterial vessels is one of the major factors of death in the present world. The aim of this study was to investigate whether the chosen herbal preparations possess thrombolytic activity or not and aimed to find out its toxicity. Methods: An in vitro thrombolytic model was used to check the clot lysis effect of the crude extract of B. pilosa, streptokinase was used as a positive control and water as a negative control. In another part, we used Brine shrimp lethality bioassay method to measure the cytotoxic potency of the plant extract. Results: In the in vitro thrombolytic model, methanolic extract of B. pilosa showed significant (p <0.002) clot lysis activity with 46.20 ± 2.274% when compared with positive control Streptokinase (82.60 ± 2.45%) and negative control distilled water (11.29 ± 0.677%). Other part of our study showed moderate or little bit low activity with LC50 of 216.7µg/ml. Conclusions: Our study suggests that thrombolytic activity of B. pilosa could be considered as very promising and beneficial for the Bangladeshi traditional medicine. Lower effects in cytotoxic activity finding may be due to insufficient quantities of toxic metabolite or antitumor component in the extract. In vivo clot dissolving property and active components of the extract for clot lysis could lead the plants for their therapeutic uses. However, further work will establish whether, the phytochemicals from this plant could be incorporated as a thrombolytic agent for the improvement of the patients suffering from diseases like atherosclerosis or embolism.



[1]  Elumalai A, Eswariah MC, Chowdary VCH, Kumar R, Anusha M and Naresh K; Screening of thrombolytic activity of Bougainvillea glabra leaves extract by In-Vitro; Asian J Res Pharm Sci; 2012; 2(4):134-136.
[2]  Emran TB, Rahman MA, Uddin MMN, Rahman MM, Dash R, Layzu C and Uddin MZ; Effects of organic extracts and their different fractions of five Bangladeshi plants on in vitro thrombolysis; BMC Compl Alt Med; 2015; 15:128-36.
[3]  Hossain MK, Hassan MM, Parvin MN, Hasan MM, Islam MS and Haque MA; Antimicrobial, cytotoxic and thrombolytic activity of Cassia senna leaves (family: Fabaceae); J App Pharm Sci; 2012; 2(6): 186-190.
[4]  Gennaro AR. Remington; The Science and Practice of Pharmacy; Thrombolytic agents; 20 th ed. Lippincott Williams & Wilkins; New York; 2000; 1256-1257.
[5]  Sweta P, Rajpal SK, Jayant YD, Hemant JP, Girdhar MD and Hatim FD; Development of an in vitro model to study clot lysis activity of thrombolytic drugs; Throm J; 2006; 4(14):1-4.
Show More References
[6]  Collen D; Coronary thrombolysis: streptokinase or recombinant tissue-type plasminogen activator; Ann Intern Med; 1990; 112: 529-538.
[7]  Marder VJ; Recombinant streptokinase – opportunity for an improved agent; Blood Coagul Fibrin; 1993; 4: 1039-1040.
[8]  Demrow HS, Slane PR, Folts JD; Administration of wine and grape juice inhibits in vivo platelet activity and thrombosis in stenosed canine coronary arteries; Circulation; 1995; 91:1182-1188.
[9]  Basta G, Lupi C, Lazzerini G, Chiarelli P, L'Abbate A, Rovai D; Therapeutic effect of diagnostic ultrasound on enzymatic thrombolysis: An in vitro study on blood of normal subjects and patients with coronary artery disease; Thromb Haemost; 2004; 91: 1078-1083.
[10]  Yamamoto J, Yamada K, Naemura A, Yamashita T and Arai R; Testing various herbs for antithrombotic effect; Nutrition; 2005; 21:580-587.
[11]  http://www.ebbd.info/byttneria-pilosa.html; cited 6 April 2015.
[12]  Islam A, Siddik AB, Hanee U, Guha A, Zaman F, Mokarroma U, Zahan H, Jabber S, Naurin S, Kabir H, Jahan S and Rahmatullah M; Ethnomedicinal practices of Chakma tribal healer practicing among a Marma community in Rangamati district, Bangladesh; World J Pharm Pharma Sci; 2015; 4(3): 180-188.
[13]  Rahman MA; Indigenous knowledge of herbal medicine in Bangladesh, 3 treatment of skin diseases by tribal communities of the hill tracts districts; Bangladesh J Bot; 2010; 39(2): 169-177.
[14]  Rahman MA, Uddin SB and Wilcock CC; Medicinal plants used by Chakma tribe in the hill tracts districts of Bangladesh; Indian J Trad Know; 2007; 6(3): 508-517.
[15]  A. Ghani; 1st ed.; Textbook of Pharmacognosy; Institution of Medical Technology, Dhaka, Bangladesh; 2005.
[16]  Rahman MA, Sultana R, Emran TB, Islam MS, Chakma JS, Rashid HU, et al; Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity; BMC Compl Alt Med; 2013; 13(25):1472-6882.
[17]  Prasad S, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM and Daginawala HF; Development of an in vitro model to study clot lysis activity of thrombolytic drugs; Thromb J; 2006; 4:14.
[18]  Goldstein AL and Kalkan SM; Principles of Drug Action; 2nd ed; Willey Biochemical Health Publications; 1974; 376-381.
[19]  Meyer BB, Ferringi NR, Futman FJ, Jacobsen LB, Nichols DE and Mclaughlin JL; Brine shrimp a convenient general bioassay for active plant constituents; Planta Medica; 1982; 5: 31-34.
[20]  Libby P, Ridker PM and Hansson GK; Progress and challenges in translating the biology of atherosclerosis; Nature; 2011; 473: 317-325.
[21]  Fuentes E, Guzmán L, Alarcón M, Moore R and Palomo I; Thrombolytic/fibrinolytic mechanism of natural products; Fibrinolysis and Thrombolysis; chapter 5; 2014; 107-121.
[22]  Ananyeva NM; Kouiavskaia DV; Shima M and Saenko EL; Intrinsic pathway of blood coagulation contributes to thrombogenicity of atherosclerotic plaque; Blood; 2002; 99: 4475-4485.
[23]  Zinkstok SM, Vermeulen M, Stam J, de Haan RJ and Roos YB; Antiplatelet therapy in combination with rt-PA thrombolysis in ischemic stroke (ARTIS): rationale and design of a randomized controlled trial; Cerebrovasc Dis; 2010; 29: 79-81.
[24]  Rahman MA, Sultana R, Bin Emran T, Islam MS; Chakma JS, Rashid HU and Hasan CM; Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity; BMC Compl Alt Med; 2013; 13: 25.
[25]  Yamada K, Naemura A, Sawashita N, Noguchi Y and Yamamoto J; An onion variety has natural antithrombotic effect as assessed by thrombosis/thrombolysis models in rodents; Thromb Res; 2004; 114: 213-220.
[26]  Suzuki Y, Kondo K, Matsumoto Y, Zhao BQ, Otsuguro K, Maeda T, Tsukamoto Y, Urano T and Umemura K; Dietary supplementation of fermented soybean, natto, suppresses intimal thickening and modulates the lysis of mural thrombi after endothelial injury in rat femoral artery; Life Sci; 2003; 73: 1289-1298.
[27]  Rajput MS, Mathur V, Agrawal P, Chandrawanshi HK and Pilaniya U; Fibrinolytic activity of kaempferol isolated from the fruits of Lagenaria siceraria (Molina) Standley; Nat Prod Res; 2011; 25: 1870-1875.
[28]  Licciardi PV and Underwood JR; Plant-derived medicines: a novel class of immunological adjuvants; Int Immunopharmacol; 2011; 11(3): 390-8.
[29]  Potterat O and Hamburger M; Drug discovery and development with plant-derived compounds; Prog Drug Res; 2008; 65(45): 47-118.
[30]  Verstraete M; Third-generation thrombolytic drugs; Am J Med; 2000; 109(1):52-8.
[31]  McLauglin JL, Chang CJ and Smith DL; Simple bench-top bioassays (brine shrimp and potato discs) for the discovery of plant antitumour compounds. In: Human Medicinal Agents from Plants; Kinghorn AD and Balandrin MF; (Eds.), ACS Symposium 534, American Chemical Society, Washington, D. C.; 1993: 112-137.
[32]  Moreira MD, Picanço MC, Barbosa LC, Guedes RN, Barros EC and Campos MR; Compounds from Ageratum conyzoides: isolation, structural elucidation and insecticidal activity; Pest Manag Sci; 2007; 63: 615-621.
[33]  Okwori AEJ, Dina CO, Junaid S, Okeke IO, Adetunji JA and Olabode AO; Antibacterial activities of Ageratum conyzoides extracts on selected bacterial pathogens; Int J Micro; 2007; 4: 1937-1949.
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