Welcome to American Journal of Biomedical Research

American Journal of Biomedical Research is a peer-reviewed, open access journal that provides rapid publication of articles in all areas of biomedical research. The goal of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of biomedical research.

ISSN (Print): 2328-3947

ISSN (Online): 2328-3955

Editor-in-Chief: Hari K. Koul

Website: http://www.sciepub.com/journal/AJBR

   

Article

Effects of Chronic Alcohol Ingestion on Hematological Parameters in Albino Mice Experimentally Challenged with Escherichia coli Strain 0157:H7

1Department of Animal Production and health, Faculty of Agriculture, Federal University, Oye-ekiti, Ekiti State, Nigeria

2Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P.M.B. 1115 Calabar, Nigeria

3Department of Fisheries and Aqua-culture, Faculty of Agriculture, Federal University, Oye-ekiti, Ekiti State, Nigeria

4Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Abuja, Nigeria

5Department of Veterinary Microbiology and Pathology, University of Nigeria, Nsukka, Enugu State, Nigeria


American Journal of Biomedical Research. 2015, 3(2), 21-28
doi: 10.12691/ajbr-3-2-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Asuzu Onyeka V., Nwaehujor Chinaka O., Okeke Onyinye S., Ode Julius O., Chah Kennedy F.. Effects of Chronic Alcohol Ingestion on Hematological Parameters in Albino Mice Experimentally Challenged with Escherichia coli Strain 0157:H7. American Journal of Biomedical Research. 2015; 3(2):21-28. doi: 10.12691/ajbr-3-2-2.

Correspondence to: Nwaehujor  Chinaka O., Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P.M.B. 1115 Calabar, Nigeria. Email: chinaka_n@yahoo.com

Abstract

The aim of this study was to investigate the effect of chronic alcohol consumption on hematology when challenged with E. coli strain 0157:H7 using albino mice as experimental model. Eight weeks old mice (26.6 – 35.3 g) of both sexes were used in the study and were divided into 6 groups of 12 mice each using stratified random selection method. Group 1 was given 10 % ethanol (V/V) in their drinking water. Group 2 received 20 % of ethanol. The third group received 30 % of ethanol while Group 4 and 5 received 40 % of alcohol ad-libitum respectively. Group 6 served as control and received only distilled water. The alcohol-treated groups received ethanol for 3 weeks to establish a chronic state of alcoholism and Groups 1-4 were then challenged with E. coli strain 0157:H7 for 7 days. Blood samples were collected via the median canthus of the eyes from the retrobublar plexus. The blood samples were allowed to clot and the sera was obtained by aspiration into Bijou bottles for hematology analysis like packed cell volume (PCV), hemoglobin (Hb), white blood cell (WBC), red blood cell (RBC), neutrophil, lymphocytes, monocytes, eosinophil, and basophil. There was a significance difference (P< 0.05) in the mean WBC, RBC, PCV and Hb values between various groups. The means of WBC of the groups exposed to 10 % alcohol with E. coli (9.2±0.1) had a significantly higher (P<0.05) value than all the other alcohol-treated groups. The mean PCV of groups exposed to 40 % alcohol with E. coli (36.67±0.88), 30 % alcohol with E. coli (37±1.08) and 20 % alcohol with E. coli (37.50±1.19) were significantly (P<0.05) less than that of groups exposed to 10 % alcohol with E. coli (39.60±1.40). Groups that consumed 10% alcohol with E. coli, water with E. coli (41.67±0.88), and water without E. coli (42.25±1.31) were significantly (P<0.05) lower than the group that consumed 40 % alcohol without E. coli challenge (46.25±0.75), but higher than the groups treated with alcohol and challenged with E. coli. There was significant difference (P< 0.05) in the RBC mean values between the various groups. The mean values of groups exposed to 40% alcohol with E. coli (899.2±116.58) and 30 % alcohol with E. coli (923.3±38.37) were significantly (P<0.05) lower than 20 % alcohol with E. coli (978.3±46.39), water with E. coli (985±31.75), water without (998.8±85.81) and 40 % alcohol without E. coli (1068.2±22.58). Neutrophil, lymphocyte and monocyte values across the various groups revealed significant differences (P< 0.05) among the different groups. The results showed that chronic alcohol (ethanol) consumption has adverse pathologic effects on the packed cell volume (PCV), hemoglobin (Hb), white blood cell (WBC), red blood cell (RBC), neutrophil, lymphocytes, monocytes, eosinophil, and basophil. Although alcohol is generally obtained from the fermentation of starch-containing food, its abuse and daily consumption causes damage hematological parameters in the body. Thus, when such a body is challenged with a pathogenic organism, there is less resistance to systemic entry of the cells by the organism, faster access to body cell due to the dehydration effect, and a quick necrotic time due to the toxins produced by such pathogenic organisms.

Keywords

References

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Article

Hepatoprotective, DNA Damage Prevention and Antioxidant Potential of Spirulina platensis on CCl4-Induced Hepatotoxicity in Mice

1Genetics Branch, Botany Department, Faculty of Agriculture, Saba Basha, Alexandria University, Egypt

2Marine Biotechnology and Natural products lab, The National Institute of Oceanography and fisheries, Egypt

3Biophysics and Laser Science Unit, Basic Science Department, Research Institute of Ophthalmology


American Journal of Biomedical Research. 2015, 3(2), 29-34
doi: 10.12691/ajbr-3-2-3
Copyright © 2015 Science and Education Publishing

Cite this paper:
AshganA. AbouGabal, Haiam M. Aboul-Ela, EmanM Ali, Ahemd E.M khaled, Ola Kh. Shalaby. Hepatoprotective, DNA Damage Prevention and Antioxidant Potential of Spirulina platensis on CCl4-Induced Hepatotoxicity in Mice. American Journal of Biomedical Research. 2015; 3(2):29-34. doi: 10.12691/ajbr-3-2-3.

Correspondence to: Haiam  M. Aboul-Ela, Marine Biotechnology and Natural products lab, The National Institute of Oceanography and fisheries, Egypt. Email: haiam_morsy@yahoo.com

Abstract

In the present study, we have evaluated the hepatoprotective and antioxidant effects of Spirulina platensis against CCl4-induced hepatotoxicity in mice. Activities of liver marker enzymes; Alanine transaminase and Aspartate transaminasewere estimated, as well as lipid peroxidation and antioxidant status (glutathione peroxidase) were determined in liver homogenate. DNA damage in liver was also evaluated by means of Comet assay. CCl4 induction (1 mg/kg b.wt) significantly increases the levels of liver marker enzymesand lipid peroxidation, and caused the depletion of antioxidant status. Treatment of Spirulinaplatensis (800mg/kg/b.wt) to CCl4 challenged mice resulted in decreased liver marker enzymes activity, DNA damage and lipid peroxidation levels with increase in antioxidant status. Our study clearly demonstrates that Spirulina platensis shows hepatoprotective effect through its antioxidant activity on CCl4-induced hepatotoxicity in mice.

Keywords

References

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Article

Evaluation of Thrombolytic and Cytotoxic activities of an Ornamental medicinal plant: Byttneria pilosa

1Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh

2Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka-1229, Bangladesh

3State University of Bangladesh, 138, Mirpur Road, Dhaka-1205, Bangladesh


American Journal of Biomedical Research. 2015, 3(3), 35-39
doi: 10.12691/ajbr-3-3-1
Copyright © 2015 Science and Education Publishing

Cite this paper:
Mubarik Yusuf Ibrahim, Prawej Ansari, AKM Riasat-ul-Islam, Mahmuda Sultana, Nadia Akter Zhumur, Shah Mohammed Shafi. Evaluation of Thrombolytic and Cytotoxic activities of an Ornamental medicinal plant: Byttneria pilosa. American Journal of Biomedical Research. 2015; 3(3):35-39. doi: 10.12691/ajbr-3-3-1.

Correspondence to: Prawej  Ansari, Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh. Email: chemist89ansari@gmail.com

Abstract

Purpose: The rapidly growing incidence of ischemic stroke caused by thrombosis of the arterial vessels is one of the major factors of death in the present world. The aim of this study was to investigate whether the chosen herbal preparations possess thrombolytic activity or not and aimed to find out its toxicity. Methods: An in vitro thrombolytic model was used to check the clot lysis effect of the crude extract of B. pilosa, streptokinase was used as a positive control and water as a negative control. In another part, we used Brine shrimp lethality bioassay method to measure the cytotoxic potency of the plant extract. Results: In the in vitro thrombolytic model, methanolic extract of B. pilosa showed significant (p <0.002) clot lysis activity with 46.20 ± 2.274% when compared with positive control Streptokinase (82.60 ± 2.45%) and negative control distilled water (11.29 ± 0.677%). Other part of our study showed moderate or little bit low activity with LC50 of 216.7µg/ml. Conclusions: Our study suggests that thrombolytic activity of B. pilosa could be considered as very promising and beneficial for the Bangladeshi traditional medicine. Lower effects in cytotoxic activity finding may be due to insufficient quantities of toxic metabolite or antitumor component in the extract. In vivo clot dissolving property and active components of the extract for clot lysis could lead the plants for their therapeutic uses. However, further work will establish whether, the phytochemicals from this plant could be incorporated as a thrombolytic agent for the improvement of the patients suffering from diseases like atherosclerosis or embolism.

Keywords

References

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