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American Journal of Biomedical Research is a peer-reviewed, open access journal that provides rapid publication of articles in all areas of biomedical research. The goal of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of biomedical research.

ISSN (Print): 2328-3947

ISSN (Online): 2328-3955

Editor-in-Chief: Hari K. Koul

Website: http://www.sciepub.com/journal/AJBR



Evaluation of Thrombolytic and Cytotoxic activities of an Ornamental medicinal plant: Byttneria pilosa

1Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh

2Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka-1229, Bangladesh

3State University of Bangladesh, 138, Mirpur Road, Dhaka-1205, Bangladesh

American Journal of Biomedical Research. 2015, 3(3), 35-39
doi: 10.12691/ajbr-3-3-1
Copyright © 2015 Science and Education Publishing

Cite this paper:
Mubarik Yusuf Ibrahim, Prawej Ansari, AKM Riasat-ul-Islam, Mahmuda Sultana, Nadia Akter Zhumur, Shah Mohammed Shafi. Evaluation of Thrombolytic and Cytotoxic activities of an Ornamental medicinal plant: Byttneria pilosa. American Journal of Biomedical Research. 2015; 3(3):35-39. doi: 10.12691/ajbr-3-3-1.

Correspondence to: Prawej  Ansari, Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh. Email: chemist89ansari@gmail.com


Purpose: The rapidly growing incidence of ischemic stroke caused by thrombosis of the arterial vessels is one of the major factors of death in the present world. The aim of this study was to investigate whether the chosen herbal preparations possess thrombolytic activity or not and aimed to find out its toxicity. Methods: An in vitro thrombolytic model was used to check the clot lysis effect of the crude extract of B. pilosa, streptokinase was used as a positive control and water as a negative control. In another part, we used Brine shrimp lethality bioassay method to measure the cytotoxic potency of the plant extract. Results: In the in vitro thrombolytic model, methanolic extract of B. pilosa showed significant (p <0.002) clot lysis activity with 46.20 ± 2.274% when compared with positive control Streptokinase (82.60 ± 2.45%) and negative control distilled water (11.29 ± 0.677%). Other part of our study showed moderate or little bit low activity with LC50 of 216.7µg/ml. Conclusions: Our study suggests that thrombolytic activity of B. pilosa could be considered as very promising and beneficial for the Bangladeshi traditional medicine. Lower effects in cytotoxic activity finding may be due to insufficient quantities of toxic metabolite or antitumor component in the extract. In vivo clot dissolving property and active components of the extract for clot lysis could lead the plants for their therapeutic uses. However, further work will establish whether, the phytochemicals from this plant could be incorporated as a thrombolytic agent for the improvement of the patients suffering from diseases like atherosclerosis or embolism.



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Evaluation of the Toxicity of Hemizygia bracteosa (Benth) Plant Used in Traditional Medicine for the Treatment of Diabetes Mellitus in Benin

1Laboratory of Teaching and Research in Food Microbiology, DSepartment of Food Technology Engineering, Polytechnic School of Abomey, University of Abomey, 01P.BP 2009 Cotonou, Benin

2Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey, University of Abomey-calavi, 01 BP 2009 Cotonou, Benin.

3Laboratory of Cytogenetic, Human Biology Unit, Faculty of Health Sciences / University of Abomey-calavi, Benin

4Association for the Promotion of Tropical Medicinal Plants (APTMP), 03 BP 246 Cotonou, Benin

American Journal of Biomedical Research. 2015, 3(3), 40-44
doi: 10.12691/ajbr-3-3-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Nicodème W CHABI, Christian T. R. KONFO, Marius ADJAGBA, Loukman MOUSSEDIKOU, Edwige AHOUSSI-DAHOUENON, Anatole LALEYE, Christophe GBAGUIDI, Mohamed Mansourou SOUMANOU. Evaluation of the Toxicity of Hemizygia bracteosa (Benth) Plant Used in Traditional Medicine for the Treatment of Diabetes Mellitus in Benin. American Journal of Biomedical Research. 2015; 3(3):40-44. doi: 10.12691/ajbr-3-3-2.

Correspondence to: Christian  T. R. KONFO, Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey, University of Abomey-calavi, 01 BP 2009 Cotonou, Benin.. Email: konfo01@yahoo.fr, nicodeme.chabi@gmail.com


This study aims to assess the toxicity of Hemizygia bracteosa (Benth), a medicinal plant used for the treatment of diabetes mellitus in Benin. Wistar rats were force-feeded by using solutions obtained by dissolving the powder of this plant in distilled water. The assessment of biochemical parameters (blood glucose, triglycerides, cholesterol, HDL cholesterol, proteins) of Wistar rat serum was used to highlight the hypoglycaemic properties of this plant and its effects on cardiovascular risk factors. The results showed an increase in HDL-cholesterol levels (0.13 ± 0.004 vs 0.48 ± 0.15) and a decrease in total cholesterol levels (1.06 ± 0.40 vs 0.81 ± 0.12), triglycerides (1.2 ± 0.43 vs 1.18 ± 0.38), glucose (1.60 ± 0.25 vs 0.98 ± 0.24) and proteins (95. 33 ± 20.59 vs 58.30 ± 6.26) in rats serum after one month treatment. No significant differences were observed in control rats for all parameters. our data also shows that at concentrations from 2 g/kg this plant appears topresent significant toxicity on kidneys and liver. The use of this plant for diabetes treatment must be done with caution and doses must be controlled.



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Effect of Variations in Post-set Temperature and Monomer Concentration on Self-cure Acrylic Surface Candidal Growth: An In-vitro Study

1Department of Dental Materials, Islamic International Dental College, Riphah University, Islamabad

2Department of Community Dentistry, Riphah International University

American Journal of Biomedical Research. 2015, 3(3), 45-52
doi: 10.12691/ajbr-3-3-3
Copyright © 2015 Science and Education Publishing

Cite this paper:
Nimra Tahir, Faisal Moeen, Muhammad Humza Bin Saeed. Effect of Variations in Post-set Temperature and Monomer Concentration on Self-cure Acrylic Surface Candidal Growth: An In-vitro Study. American Journal of Biomedical Research. 2015; 3(3):45-52. doi: 10.12691/ajbr-3-3-3.

Correspondence to: Faisal  Moeen, Department of Dental Materials, Islamic International Dental College, Riphah University, Islamabad. Email: faisalmoeen_is@yahoo.com, faisal.moeen@riphah.edu.pk


Objectives: To identify the effect of variations in power-liquid ratio and post-set temperature on water absorption and residual monomer concentration on growth of Candida albicans on self-cure denture base resins. Methodology: 60 self-cured acrylic discs (39 x 4mm) were made and divided into 3 groups each having 20 specimens. Group 1 consisted of discs fabricated at a powder-liquid ratio of 5.1:2.8 as recommended by the manufacturer. Groups 2 and 3 constituted specimens with variations in post-set temperatures and powder-liquid ratios respectively. Specimens from group 2 were soaked in four water baths at temperatures of 37°C, 47°C, 57°C and 67°C for 24 hours. Specimens from the temperature-controlled group 3 were fabricated at four different powder-liquid ratios by increasing monomer liquid volume by 10%, 15%, 20% and 25%. The acrylic discs from control groups 1 and 3 were soaked at a constant temperature of 37°C for 24 hours. Residual monomer leeched out from disc into water was analyzed using Ultraviolet spectrophotometer. The variations in water absorption and Candidal growth were recorded. Results: Linear regression analysis was used to analyze the results. A moderately positive correlation was calculated for the association between powder-liquid ratio and Candidal growth (r = 0.67, p < 0.001) suggesting Candidal growth is higher at increased powder-liquid ratios having low monomer content. The correlation between the Candidal growth and the post-set temperatures was found to be moderately negative (r = -0.41 p < 0.001), indicating a decrease in Candidal cells on increasing post-set soaking temperatures. Conclusions: Candidal growth follows a positive linear relation with a decreasing powder-liquid ratio having cells increasing with an increase in liquid monomer content. Candidal growth follows a negative linear relation with post-set temperature soaking with cells decreasing with an increase in temperature.



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