ISSN (Print): 2328-3947

ISSN (Online): 2328-3955

Editor-in-Chief: Hari K. Koul

Website: http://www.sciepub.com/journal/AJBR

   

Article

Review of FBN1 Gene Role in Marfan Syndrome Presentations Insilico Analysis

1Departement of human anatomy faculty of Medicine University of Khartoum - Sudan

2Daoud research group

3MBBS university of Khartoum

4Division of Molecular Genetics, university of Tubingen,- Germany

5Deprtement of Surgery University of Khartoum - Sudan

6Medical Student University of Khartoum - Sudan

7Institute of endemic diseases - Khartoum - Sudan


American Journal of Biomedical Research. 2016, 4(1), 5-12
doi: 10.12691/ajbr-4-1-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Mohanad Abdelrahim, Khalid EL.Khalid, Mohammed Elamin Faris, Mohamed A.Hassan, Kamal Elsiddig, Ahmed Siddig Muhammed, Mohammed Nimir, Mahil Abdalla, Asgad Suliman. Review of FBN1 Gene Role in Marfan Syndrome Presentations Insilico Analysis. American Journal of Biomedical Research. 2016; 4(1):5-12. doi: 10.12691/ajbr-4-1-2.

Correspondence to: Mohanad  Abdelrahim, Departement of human anatomy faculty of Medicine University of Khartoum - Sudan. Email: mohanadkhalid91@gmail.com

Abstract

Overview: This is translational bioinformatics was focused on analysis of single nucleotide polymorphism of FBN1 gene and reviewing of the previous citations of the damaging SNPs. Introduction: Marfan syndrome is a common autosomal dominant hereditary connective tissue disorder with variable presentations, mutations in FBN1 gene were found to be responsible for Marfan syndrome and other related connective tissue disorders, SNPs contributes to gene mutations and expression variations justifying phenotypic variations among patients and hence such SNPs would be potential target for identification and analysis which may help in early diagnosis of such life threatening disorder. Methods: computational methods were used on this work focusing on analysis of SNPs in the coding regions of FBN1 gene found as non-synonymous variants (ns-SNP) and those in the 3’un-translated regions (3’UTR) affecting miRNA binding using computational methods including SIFT and polyphen for analysis of (nsSNPs), prediction of not previously described SNPs was done using project hope software, while (3’UTR) SNPs was analyzed using PolymiRTS tool functions interactions of FBN1 gene with functionally similar gene were predicted using Genemania software. Results: Out of 1134 ns-SNPs analyzed 38 SNPs were found to damaging while analysis of 175 SNP in 3’UTR prove that 24 SNPs are disturbing to their target sites and 46 SNPs are creating to new target sites.On reviewing of previous citation 31 of the predicted damaging nSNPs were reported as mutations with specific Marfan syndrome presentation while 6 nsSNP were not previously reported with high damaging probability.

Keywords

References

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Article

Assessment of Anti-ulcer Efficacy of Stem Bark Extract of Nauclea latifolia (African Peach) in Rats

1Department of Physiology, Faculty of Medicine, College of Health Sciences, Ebonyi State University, Abakaliki, Nigeria

2Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Nigeria Enugu Campus, Nigeria

3Department of Physiology, Faculty of Basic Medical Sciences, Lagos State University College of Medicine, Ikeja, Lagos State, Nigeria

4Department of Paediatrics, Faculty of Medicine, College of Health Sciences, Federal Teaching Hospital, Abakaliki, Nigeria


American Journal of Biomedical Research. 2016, 4(1), 13-17
doi: 10.12691/ajbr-4-1-3
Copyright © 2016 Science and Education Publishing

Cite this paper:
Morufu Eyitayo Balogun, Daniel Chukwu Nwachukwu, Shakiru Ademola Salami, Elizabeth Enohnyaket Besong, Dorathy Chinwe Obu, Serges Fankou Athanase Djobissie. Assessment of Anti-ulcer Efficacy of Stem Bark Extract of Nauclea latifolia (African Peach) in Rats. American Journal of Biomedical Research. 2016; 4(1):13-17. doi: 10.12691/ajbr-4-1-3.

Correspondence to: Morufu  Eyitayo Balogun, Department of Physiology, Faculty of Medicine, College of Health Sciences, Ebonyi State University, Abakaliki, Nigeria. Email: marufbalogun@yahoo.com

Abstract

Aims: Nauclea latifolia Sm. (Rubiaceae) is known for its therapeutic uses, especially in alternative medicine. Its leaves, roots and bark have been used traditionally in Nigeria to treat various diseases including ulcer-related diseases. Methods: The aqueous stem bark extract of Nauclea latifolia was investigated for anti-ulcerogenic effects using ethanol/HCI and indomethacin as the ulcerogens. The effect of the extract on gastric mucous secretion was also investigated. The extract was administered orally at the doses of 100 and 200 mg/kg b. wt. for the experimental groups while the control and reference groups received distilled water (2 ml/kg, p.o) and omeprazole (20 mg/kg, p.o) respectively. Results: Phytochemical studies revealed the presence of saponins, tannins, alkaloids, terpenes and glycosides. The results show that the extract significantly (p < 0.05) reduced the ulcer index from 4.55 ± 1.45 to 1.20 ± 0.19 and from 4.20 ± 0.72 to 0.94 ± 2.51 in the ethanol/HCI and indomethacin induced ulceration respectively. The extract also significantly (p < 0.05) increased the gastric mucous secretion in a dose-dependent manner. Conclusions: The results suggest that the N. latifolia stem bark extract possesses significant anti-ulcer effects which might be due to its ability to increase gastric mucous secretion.

Keywords

References

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Article

Effects of Selenium Treatment on Healing of Acetic Acid Induced Gastric Ulcer in Albino Wistar Rats

1Department of Human Physiology, College of Health Sciences, Kogi State University, Anyigba. Post code: 272102, Nigeria

2Department of Biochemistry, Faculty of Natural Sciences, Kogi State University, Anyigba. Post code: 272102, Nigeria


American Journal of Biomedical Research. 2016, 4(1), 18-22
doi: 10.12691/ajbr-4-1-4
Copyright © 2016 Science and Education Publishing

Cite this paper:
Adeniyi Olasupo Stephen, Omale James, Egwuje Rita Ikoojo, Ajayi Olugbenga Sunday. Effects of Selenium Treatment on Healing of Acetic Acid Induced Gastric Ulcer in Albino Wistar Rats. American Journal of Biomedical Research. 2016; 4(1):18-22. doi: 10.12691/ajbr-4-1-4.

Correspondence to: Adeniyi  Olasupo Stephen, Department of Human Physiology, College of Health Sciences, Kogi State University, Anyigba. Post code: 272102, Nigeria. Email: supoadeniyi@yahoo.com

Abstract

The effects of selenium on healing of acetic acid induced gastric ulcer investigated in albino Wistar rats and the results were compared with that of a standard drug omeprazole. Animals were anesthetized with 50mg/kg sodium thiopental, laparotomy was performed and gastric ulcer was induced by application of 80% acetic acid to the serosal surface of stomach. Animals were then divided into three groups. Group 1 was treated with 1 ml/kg/day normal saline (NS) (p.o), group 2; 100µg/kg/day selenium (p.o) and group 3; 20mg/kg/day omeprazole (p.o). Treatment period was 10 days post ulcer induction. Assessment of ulcer healing was done on days 3, 7 and 10 respectively by measurement of ulcer area, lipid peroxidation, catalase activity and mucus secretion. Result showed that by day 7 and 10, the reduction in ulcer area was more significant in selenium and omeprazole treated (p< 0.05, 0.05) respectively. By day 7 and 10, lipid peroxidation was significantly lower in selenium and omeprazole treated as compared with NS treated (p<0.05, 0.05). Furthermore by day 3, 7 and 10, catalase activity was significantly higher (p < 0.05) in selenium treated as compared with NS treated. Result also showed that mucus secretion was significantly higher in selenium and omeprazole treated (p < 0.001, 0.001) compared with NS by day 7. However, by day 10, secretion in both selenium and omeprazole treated had started to decline. In conclusion, selenium accelerated ulcer healing by facilitating mucosal regeneration, reducing lipid peroxidation, increasing antioxidant activity and by altering mucus secretion response.

Keywords

References

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