World Journal of Agricultural Research

ISSN (Print): 2333-0643

ISSN (Online): 2333-0678

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Efficacy Assessment of Treatment Methods against Powdery Mildew Disease of Pea (Pisum sativum L.) Caused by Erysiphe pisi var. pisi

1Tribhuwan University (T. U.), Nepal

2Institute of Agriculture and Animal Science (IAAS)

World Journal of Agricultural Research. 2015, 3(6), 185-191
doi: 10.12691/wjar-3-6-1
Copyright © 2015 Science and Education Publishing

Cite this paper:
Ateet Maharjan, Bhupendra Bhatta, Raju Prasad Acharya, Sagar G. C., Swati Shrestha. Efficacy Assessment of Treatment Methods against Powdery Mildew Disease of Pea (Pisum sativum L.) Caused by Erysiphe pisi var. pisi. World Journal of Agricultural Research. 2015; 3(6):185-191. doi: 10.12691/wjar-3-6-1.

Correspondence to: Ateet  Maharjan, Tribhuwan University (T. U.), Nepal. Email:


The experiment was conducted to find out the efficacy of different treatments against powdery mildew of pea (Erysiphepisi var. pisi) in Rampur, Chitwan, Nepal during Dec. 2014 to April 2015. Four treatments viz. Skimmed cow milk (250 ml/plot), Karathane (2 ml/L), Trichoderma (107condia/ml) and Control (distilled water) were used as foliar spray on plants. Considering Disease severity (%), Disease control (%) and Total Area under Disease Progress Curve, Trichoderma was found to be effective than Skimmed cow milk and Control but was at par with Karathane, in controlling over Powdery mildew disease. Number of pods/plant and weight of grains/plant were observed highest in Trichodermawhich was followed by Karathane. There was no significant difference between treatments in case of weight of pods/plant and dry weight/plant. AUDPC/day value increased in control in different sprayings but AUDPC value/day initially increased and later decreased for Trichoderma, Karathane and skimmed cow milk. All the yield and yield attributing characters were negatively correlated with AUDPC but number of pods/plant and weight of pods/plant were positively correlated. Reduction in yield was also caused by infection of Rust (Uromycespisi) in field. Four foliar sprays of Trichoderma (107condia/ml) or Karathane@ 0.2% at interval of 7 days may be the option for the management of powdery mildew in severe condition. Trichoderma can be alternative method for farmers to have eco-friendly management of Powdery mildew as Karathane has negative impact on human and plant health.



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Molecular Diversity Assessment in Chickpea through RAPD and ISSR Markers

1School of Studies in Biotechnology, Jiwaji University, Gwalior, India

2ICAR-Indian Institute of Pulses Research, Kanpur, India 3Department of Bioscience & Biotechnology, Banasthali University, India

32ICAR-Indian Institute of Pulses Research, Kanpur, India 3Department of Bioscience & Biotechnology, Banasthali University, India

World Journal of Agricultural Research. 2015, 3(6), 192-197
doi: 10.12691/wjar-3-6-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Sameer. S. Bhagyawant, Neha Gupta, Ajay Gautam, S.K. Chaturvedi, Nidhi Shrivastava. Molecular Diversity Assessment in Chickpea through RAPD and ISSR Markers. World Journal of Agricultural Research. 2015; 3(6):192-197. doi: 10.12691/wjar-3-6-2.

Correspondence to: Nidhi  Shrivastava, 2ICAR-Indian Institute of Pulses Research, Kanpur, India 3Department of Bioscience & Biotechnology, Banasthali University, India. Email:


Chickpea (Cicer arietinum L.) is a cool season food legume in the family of Fabaceae. The exploration of genetically variable accessions is the key source of germplasm conservation for future selecting parents for breeding. PCR based analysis employing RAPD and ISSR marker are one of the established techniques of estimation of genetic diversity. Twelve chickpea accessions were analyzed by ten RAPD and ISSR primers of which all primers gave amplification products. The results showed that the level of DNA polymorphism in these accessions is low. RAPD analysis yielded 5.9 bands per primer while ISSR analysis produce 2.8 bands per primer. The average percentage of molecular polymorphism as produced by RAPD and ISSR primers was 67%. The cluster dendrogram demonstrated a similarity coefficient range of 0.10 to 0.66 due to RAPD markers, whereas ISSR markers showed 0.60 to 0.80. The variability index worked out in the present study ranges from 0.49 to 0.78. The present study determines DNA polymorphism in chickpea accessions as revealed by RAPD and ISSR markers. Overall the study ascertained that RAPD and ISSR marker provide powerful tools in revealing genetic diversity in chickpea.



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Susceptibility of Rubber (Hevea brasiliensis) Clones to Neofusicoccum ribis

1Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia

2Latex Plants Programme, Institute of Agricultural research for Development (IRAD). Ekona Regional Centre, Buea, Cameroon;Ministry of Scientific Research and Innovation, Regional Center of Research and Innovation for the Littoral Region, Douala, Cameroon

3Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia

4Phytopathology Unit of CEREPAH La Dibamba, Institute of Agricultural Research for Development (IRAD), Cameroon

5Latex Plants Programme, Institute of Agricultural research for Development (IRAD). Ekona Regional Centre, Buea, Cameroon

World Journal of Agricultural Research. 2015, 3(6), 198-202
doi: 10.12691/wjar-3-6-3
Copyright © 2015 Science and Education Publishing

Cite this paper:
Nyaka Ngobisa A.I.C., Zainal Abidin M.A., Wong M.Y., Abbas Nasehi, Ntsomboh Ntsefong Godswill, Owona Ndongo Pierre-Andre. Susceptibility of Rubber (Hevea brasiliensis) Clones to Neofusicoccum ribis. World Journal of Agricultural Research. 2015; 3(6):198-202. doi: 10.12691/wjar-3-6-3.

Correspondence to: Wong  M.Y., Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia. Email:


The aim of this study was to evaluate the ability of Neofusicoccum ribis to infect leaf surfaces of different rubber (Hevea brasiliensis) clones. Neofusicoccum ribis isolates previously identified on the basis of morphological characteristics and DNA sequence analysis were used to inoculate rubber leaves and seedlings in vitro and in vivo respectively. Neofusicoccum ribis isolates were demonstrated to cause lesions on rubber clones examined in this study. There was variation in susceptibility of the rubber clones to the pathogen. This study provides useful information that could be exploited to better manage the disease.



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