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Article

Inhibitory Effect of Tea (Camellia Sinensis (L.) O. Kuntze, Theaceae) Flower Extracts on Oleic Acid-Induced Hepatic Steatosis in Hepg2 Cells

1Department of Tea Science, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, China

2Department of Applied Engineering, Zhejiang Economic and Trade Polytechnic, Hangzhou, China


Journal of Food and Nutrition Research. 2014, 2(10), 738-743
DOI: 10.12691/jfnr-2-10-13
Copyright © 2014 Science and Education Publishing

Cite this paper:
Xinghai Zhang, Ying Gao, Jinwei Xu, Xiaohui Liu, Feng Jin, Bo Li, Youying Tu. Inhibitory Effect of Tea (Camellia Sinensis (L.) O. Kuntze, Theaceae) Flower Extracts on Oleic Acid-Induced Hepatic Steatosis in Hepg2 Cells. Journal of Food and Nutrition Research. 2014; 2(10):738-743. doi: 10.12691/jfnr-2-10-13.

Correspondence to: Youying  Tu, Department of Tea Science, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, China. Email: youytu@zju.edu.cn

Abstract

Tea (Camellia sinensis (L.) O. Kuntze, Theaceae) flowers possess many physiological functions and have been used in traditional medicines for deodorization, skin care, cough suppressant and expectorant in China. However, there is little information about its effect on nonalcoholic fatty liver disease (NAFLD). In this study, an oleic acid-induced HepG2 cell model of steatosis was established, and the anti-NAFLD effects of tea flowers and the related mechanisms were investigated. Three tea flower extracts, 40% TFE, 80% TFE and TFRE couldn’t prevent triglyceride (TG) accumulation in oleic acid-treated HepG2 cells (p > 0.05), but significantly decreased the TG level in lipid-overloaded HepG2 cells after 48 h treatment (p < 0.05). RT-PCR analysis revealed that three tea flower extracts did not affect the mRNA levels of peroxisome proliferator-activated receptors α (PPARα) and Acyl-CoA oxidase-1 (ACOX-1) (p > 0.05), but up-regulated the mRNA level of carnitine palmitoyl-CoA transferase (CPT) (p < 0.05). Moreover, tea flower extracts could significantly reduce intracellular reactive oxygen species (ROS) level (p < 0.05). These results indicated tea flowers may be a potential natural resourse for the treatment of NAFLD.

Keywords

References

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Article

Fatty Acid Profile Differences among the Muscle Tissue of Three Rodents (Hydrochoerushydrochaeris, Cuniculuspaca and Caviaporcellus) and Onelagomorph (Oryctolaguscuniculus)

1Universidad de La Salle, Facultad de Ciencias Agropecuarias, Departamento de Producción Animal, Bogotá, Colombia

2Universidad Nacional de Colombia, Facultad de Medicina Veterinaria y de Zootecnia, Departamento de Salud Animal, Laboratorio de Toxicología, Bogotá, Colombia


Journal of Food and Nutrition Research. 2014, 2(10), 744-748
DOI: 10.12691/jfnr-2-10-14
Copyright © 2014 Science and Education Publishing

Cite this paper:
Liliana Betancourt, Gonzalo JairDiaz. Fatty Acid Profile Differences among the Muscle Tissue of Three Rodents (Hydrochoerushydrochaeris, Cuniculuspaca and Caviaporcellus) and Onelagomorph (Oryctolaguscuniculus). Journal of Food and Nutrition Research. 2014; 2(10):744-748. doi: 10.12691/jfnr-2-10-14.

Correspondence to: Liliana  Betancourt, Universidad de La Salle, Facultad de Ciencias Agropecuarias, Departamento de Producción Animal, Bogotá, Colombia. Email: lcbetancourt@unisalle.edu.co

Abstract

The consumerdem and for functional food with nutraceutical properties has increased in recent years, and the food’s fatty acid profile is one of the factors that define its potential value asa functional food. The aim of the present study was to compare fatty acids composition of semitendinosus muscle in two native rodents, the capybara (Hydrochoerushydrochaeris) and lowland paca (Cuniculuspaca) and two domesticated species, one rodent, the guinea pig (Caviaporcellus) and one lagomorph, the common rabbit (Oryctolaguscuniculus). The animals were fed with forage-based diet. The fatty acid composition in lyophilized tissue was determined by gas chromatography. The capybara, lowland paca and guinea pig had the highest levels of omega-3 (n-3) fatty acids, predominantly α-linolenic acid (p<0.05). The guinea pig and common rabbit showed a higher content of linoleic acid (18:2 n-6) (p<0.05). The n-6: n-3 fatty acids ratio was higher to the common rabbit (p<0.05) with 8.9, in contrast, capybara and lowland paca had the lowest ratio, with lower than zero values indicating a predominance of n-3 fatty acids. These results were presented without any diet manipulation. The guinea pig meat contained an adequate balance of n-6 to n-3 fatty acids (< 4) whereas in rabbit meat there was an excess of n-6 fatty acids. The capybara and lowland paca can be considered as sources of n-3 fatty acids, represented by α-linolenic acid (18:3 n-3). These results confirm that native species not subjected to genetic selection and intensive feeding, showed higher content of n-3 fatty acids.

Keywords

References

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Article

Effects of Food Processing on the Nutrient Composition of Pyropia yezoensis Products Revealed by NMR-based Metabolomic Analysis

1School of Marine Sciences, Ningbo University, Ningbo, China

2State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China


Journal of Food and Nutrition Research. 2014, 2(10), 749-756
DOI: 10.12691/jfnr-2-10-15
Copyright © 2014 Science and Education Publishing

Cite this paper:
Yangfang Ye, Rui Yang, Yongjiang Lou, Juanjuan Chen, Xiaojun Yan, Huiru Tang. Effects of Food Processing on the Nutrient Composition of Pyropia yezoensis Products Revealed by NMR-based Metabolomic Analysis. Journal of Food and Nutrition Research. 2014; 2(10):749-756. doi: 10.12691/jfnr-2-10-15.

Correspondence to: Xiaojun  Yan, School of Marine Sciences, Ningbo University, Ningbo, China. Email: yanxiaojun@nbu.edu.cn

Abstract

The laver product is processed from fresh thallus of Pyropia yezoensis by washing, cutting, roasting, and seasoning. The nutrient composition of raw materials, semi-finished products, and finished products of P. yezoensis was systematically characterized using NMR spectroscopy and multivariate data analysis. The results showed that the nutrient composition of P. yezoensis was dominated by 11 amino acids, 11 carboxylic acids, four choline metabolites, and four sugars. The seasoning unsurprisingly caused a significant elevation in the levels of sucrose, glucose, and glutamate in the finished products, up to 38.67 ± 4.91 mg/g, 4.22 ± 0.55 mg/g, and 17.60 ± 1.93 mg/g, respectively. However, other food processing procedures such as washing and roasting may be also responsible for widespread changes of nutrient composition including amino acids, carboxylic acids, choline metabolites, laminitol, floridoside, and isofloridoside from raw materials to finished products of seaweed. Typically, the levels of choline-O-sulfate and isofloridoside were respectively decreased to 5.93 ± 0.86 mg/g and 0.67 ± 0.09 mg/g after food processing. These findings offer essential information for the effects of food processing on the nutrient composition of seaweed products and demonstrate that NMR-based metabolomic strategy is of important values for understanding the effects of food processing on the quality and taste of seaweed products.

Keywords

References

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Article

Walnut Polyphenols Inhibit Pancreatic Lipase Activity in Vitro and Have Hypolipidemic Effect on High-Fat Diet-Induced Obese Mice

1Faculty of life science, Kunming University of Science and Technology, Kunming, People's Republic of China

2Yunnan University of Traditional Chinese Medicine, Kunming, People's Republic of China


Journal of Food and Nutrition Research. 2014, 2(10), 757-763
DOI: 10.12691/jfnr-2-10-16
Copyright © 2014 Science and Education Publishing

Cite this paper:
Dandan Shi, Chaoyin Chen, Shenglan Zhao, Feng Ge, Diqiu Liu, Hao Song. Walnut Polyphenols Inhibit Pancreatic Lipase Activity in Vitro and Have Hypolipidemic Effect on High-Fat Diet-Induced Obese Mice. Journal of Food and Nutrition Research. 2014; 2(10):757-763. doi: 10.12691/jfnr-2-10-16.

Correspondence to: Shenglan  Zhao, Yunnan University of Traditional Chinese Medicine, Kunming, People's Republic of China. Email: chaoyinchen@163.com;zhaoshenglan@163.com

Abstract

This study was aimed at the chemical composition and inhibitory effects of walnut polyphenols (WP) in vitro lipase activity and on obesity in obese mice models. More than 20 individual phenolics such as gallotannins, ellagitannins, flavonoids and phenolic acids were identified in WP. Studying the interactions between porcine pancreatic lipase (PL) and WP were based on fluorescence quenching and an enzymatic assay. The addition of WP to lipase caused a reduction of protein fluorescence intensity at 310 K. In addtion, we found that the effect of WP on PL was dependent on reaction medium and substrate used and the half maximal inhibitory concentration of WP was determined to be 163 μg/mL. At the beginning of the experiment, mice were divided into 3 groups, one of them served as normal control group (NCG), the second as hyperlipidemia control group (HCG), the last as walnut polyphenol-treated group (WTG). After 8 weeks of treatment, we investigated the effects of WP on weight gain, food intake and biochemical indexes in obese mice. The results showed that WP could significantly decrease body weight in obese mice (-13.52%, P < 0.05). However, there was no remarkable difference in food intake among three groups (1760.2–1823.3 g). In addition, WP could significantly decrease the TG, TC and LDL-cholesterol (LDL-C) concentrations and increase the HDL-cholesterol (HDL-C) concentration when compared to the HCG (-36.12%, -31.27%, -73.3%, and +59.72%, respectively, P < 0.01). The levels of hepatic TG and TC were significantly decrease in WTG when compared to the HCG (-27.72% and -48.43%, respectively, P < 0.01). In conclusion, these results suggest that WP could be a potentially therapeutic alternative in the treatment of obesity caused by a high-fat diet.

Keywords

References

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Article

Dose Water Extract of Cinnamon (Cinnamomum Zeylanicum) Exhibits Anti-Diabetic Properties in Cultured 3T3-L1 Adipocytes: A Concurrent Assessment of Adipogenesis, Lipolysis and Glucose Uptakes

1Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmed Shah, Bandar Indera Mahkota Kuantan, 25200 Pahang Darul Makmur, MALAYSIA

2Non Communicable Diseases Research Unit, Kulliyyah of Medicine, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang Darul Makmur, MALAYSIA;International Institute for Halal Research and Training (INHART), E5 2-2, Level 2, Block E5, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), P.O. Box 10 Kuala Lumpur, MALAYSIA

3Non Communicable Diseases Research Unit, Kulliyyah of Medicine, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang Darul Makmur, MALAYSIA

4International Institute for Halal Research and Training (INHART), E5 2-2, Level 2, Block E5, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), P.O. Box 10 Kuala Lumpur, MALAYSIA;Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang Darul Makmur, MALAYSIA

5Department of Basic Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang Darul Makmur, MALAYSIA


Journal of Food and Nutrition Research. 2014, 2(11), 764-769
DOI: 10.12691/jfnr-2-11-1
Copyright © 2014 Science and Education Publishing

Cite this paper:
Nuraniza Azahari, Muhammad Muzaffar Ali Khan Khattak, Muhammad Taher, Solachuddin Jauhari Arief Ichwan. Dose Water Extract of Cinnamon (Cinnamomum Zeylanicum) Exhibits Anti-Diabetic Properties in Cultured 3T3-L1 Adipocytes: A Concurrent Assessment of Adipogenesis, Lipolysis and Glucose Uptakes. Journal of Food and Nutrition Research. 2014; 2(11):764-769. doi: 10.12691/jfnr-2-11-1.

Correspondence to: Muhammad  Muzaffar Ali Khan Khattak, Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmed Shah, Bandar Indera Mahkota Kuantan, 25200 Pahang Darul Makmur, MALAYSIA. Email: mkbiol@yahoo.com

Abstract

Cinnamon is a common spice which is widely advocated for its efficacy controlling of hyperglycemia. However, the mechanism of action has yet to be established for the effect on the blood glucose concentration. Cinnamon was extracted with water and induced to cell line namely 3T3-L1 preadipocyte (American Type cell Culture Collection). The cell proliferation, differentiation (adipogenesis) and glucose uptake activity were assessed by measuring the uptake of radio-labelled glucose. Furthermore, for comparison glucose oxidase assay was also performed by using Glucose Assay Kit (Sigma-Aldrich, Inc). The collected data was statistically analysed using SPSS version 12.0 for any possible differences. This study revealed that there was a significant (P<0.01) increase the activity/adipogenesis. This increase activity was accompanied by significant (P<0.01) increase in the glucose uptake and reduced catalytic activity of lipids (lipolysis). The finding reveals that C. Zeylanicum extract has similar effect to that of insulin activity. The present study suggests that the cinnamon enhances glucose uptakes, reduces the lipid breakdown and resembles insulin activity.

Keywords

References

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Article

Mung Bean Protein Increases Plasma Cholesterol by up-regulation of Hepatic HMG-CoA Reductase, and CYP7A1 in mRNA Levels

1Institute of Crop Science, Chinese Academy of Agricultural Sciences, No. 80 South Xueyuan Road, Haidian District, Beijing, China


Journal of Food and Nutrition Research. 2014, 2(11), 770-775
DOI: 10.12691/jfnr-2-11-2
Copyright © 2014 Science and Education Publishing

Cite this paper:
Yang Yao, Yingying Zhu, Guixing Ren. Mung Bean Protein Increases Plasma Cholesterol by up-regulation of Hepatic HMG-CoA Reductase, and CYP7A1 in mRNA Levels. Journal of Food and Nutrition Research. 2014; 2(11):770-775. doi: 10.12691/jfnr-2-11-2.

Correspondence to: Guixing  Ren, Institute of Crop Science, Chinese Academy of Agricultural Sciences, No. 80 South Xueyuan Road, Haidian District, Beijing, China. Email: renguixing@caas.cn

Abstract

Interest in mung bean as a cholesterol-lowering functional food is growing. The objective of this study was to investigate the effect of mung bean protein on the blood cholesterol level and gene expression of cholesterol-regulating enzymes in Golden Syrian hamsters maintained on a 0.1% cholesterol diet. Results showed that mung bean protein (MBP) reduced plasma total cholesterol (TC), triacylglycerols and non-high-density lipoprotein cholesterol (non-HDL) concentrations as well as hepatic cholesterol concentrations after 6 week treatment. Real-time polymerase chain reaction analyses demonstrated mung bean protein could up-regulate the production of mRNA3-hydroxy-3-methyl glutaryl coenzyme A (HMG-CoA) reductase (HMGR) and cholesterol-7α-hydroxylase (CYP7A1) levels. The mechanisms underlying the cholesterol-lowering activity of MBP were mediated most likely by increasing the sterol excretion and decreasing the cholesterol absorption and synthesis. It is concluded that mung bean protein possesses the decreases plasma cholesterol activity and can be further explored as a functional food.

Keywords

References

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Article

Ethanol Extract of Polygonatumofficinale Rhizome Inhibits Odorant-Induced Camp and Calcium Levels in Non-Chemosensory 3T3-L1 Cells

1Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea


Journal of Food and Nutrition Research. 2014, 2(11), 776-780
DOI: 10.12691/jfnr-2-11-3
Copyright © 2014 Science and Education Publishing

Cite this paper:
Yeo Cho Yoon, Sung-Hee Kim, Jin-Taek Hwang, Mi Jeong Sung, Myung-Sunny Kim, Haeng Jeon Hur, Mee Ra Rhyu, Jae-Ho Park. Ethanol Extract of Polygonatumofficinale Rhizome Inhibits Odorant-Induced Camp and Calcium Levels in Non-Chemosensory 3T3-L1 Cells. Journal of Food and Nutrition Research. 2014; 2(11):776-780. doi: 10.12691/jfnr-2-11-3.

Correspondence to: Jae-Ho  Park, Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea. Email: jaehoparkmail@gmail.com

Abstract

Polygonatum Officinalerhizome, a member of the liliaceae family, is commonly consumed as tea in Asia. It is also clinically used to treat obesity and fatigue in Korean traditional medicine. Although the anti-diabetic effect of POR has been described, little is known about its physiological role in the olfactory system. In this study, we investigated the effects of POR in 3T3-L1 cells expressing an odorant receptor. We have shown that the levels of cAMP and Ca2+ and the phosphorylation of Rap1A and CREB increased in response to an odorant, eugenol. POR significantly decreased the eugenol-induced increase in cAMP and Ca2+. Taken together, these data suggest that POR inhibits an odorant-induced signal transduction pathway.

Keywords

References

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Article

HPLC/PDA–ESI/MS Identification of Phenolic Acids, Flavonol Glycosides and Antioxidant Potential in Blueberry, Blackberry, Raspberries and Cranberries

1Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Romania, Calea Mănăştur 3-5, Cluj-Napoca, Romania

2Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Romania, Calea Mănăştur 3-5, Cluj-Napoca, Romania


Journal of Food and Nutrition Research. 2014, 2(11), 781-785
DOI: 10.12691/jfnr-2-11-4
Copyright © 2014 Science and Education Publishing

Cite this paper:
ZORIŢA DIACONEASA, RANGA FLORICA, DUMITRIŢA RUGINĂ, CUIBUS LUCIAN, CARMEN SOCACIU. HPLC/PDA–ESI/MS Identification of Phenolic Acids, Flavonol Glycosides and Antioxidant Potential in Blueberry, Blackberry, Raspberries and Cranberries. Journal of Food and Nutrition Research. 2014; 2(11):781-785. doi: 10.12691/jfnr-2-11-4.

Correspondence to: CARMEN  SOCACIU, Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Romania, Calea Mănăştur 3-5, Cluj-Napoca, Romania. Email: zorita.sconta@usamvcluj.ro; carmen.socaciu@usamvcluj.ro

Abstract

Berry fruits are known to be a rich source of natural compounds which provide them many health benefits. The most common compounds that occur in berries are vitamins, flavonoids, anthocyanins and phenolic acids. The objective of this work was to identify and compare the phenolic acids, flavonol glycosides content and antioxidant potential in blueberry, blackberry, raspberries and cranberries. Berries methanolic extracts were analyzed by HPLC/PDA–ESI/MS. Antioxidant activity was determinate using FRAP assay. Antioxidant activity of analyzed berries varied considerably. Blueberry extract demonstrated the highest ferric reducing antioxidant potential (48.3 μM Fe2/g) while lower values were obtained for cranberries extract (19.6 μM Fe2/g). Berries extracts were characterized by the presence of 16 compounds in different ratios. Blueberry extract was characterized by the presence of a large amount of phenolic acids such as chlorogenic and caffeic acid while raspberries extract was found to have high amount of ellagic acid. All analyzed berries contain higher levels of bioactive compounds which are responsible for their antioxidant potential. Based on quantitative analysis of phenolics these fruits can be highly recommended for daily consumption.

Keywords

References

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Article

Determination of Organic Acids in Brassica Rapa L. Leaves (Turnip Greens and Turnip Tops) Regulated by the Protected Geographical Indication “Grelos De Galicia”

1Áreas de Nutrición y Bromatología y de Tecnología de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Santiago de Compostela, Facultad de Ciencias, Campus de Lugo, Lugo, Spain


Journal of Food and Nutrition Research. 2014, 2(11), 786-791
DOI: 10.12691/jfnr-2-11-5
Copyright © 2014 Science and Education Publishing

Cite this paper:
Mª Dolores Arias-Carmona, Mª Ángeles Romero-Rodríguez, Mª Lourdes Vázquez-Odériz. Determination of Organic Acids in Brassica Rapa L. Leaves (Turnip Greens and Turnip Tops) Regulated by the Protected Geographical Indication “Grelos De Galicia”. Journal of Food and Nutrition Research. 2014; 2(11):786-791. doi: 10.12691/jfnr-2-11-5.

Correspondence to: Mª  Lourdes Vázquez-Odériz, Áreas de Nutrición y Bromatología y de Tecnología de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Santiago de Compostela, Facultad de Ciencias, Campus de Lugo, Lugo, Spain. Email: lourdes.vazquez@usc.es

Abstract

A rapid high-performance liquid chromatographic method for the determination of organic acids in Brassica rapa L. leaves is reported. Under optimum conditions the detection limits were between 0.26 to 8.00 µg/mL and quantification limits were between 0.28 to 12.50 µg/mL. The precision results showed that the relative standard deviations of repeatability and reproducibility were ≤ 0.84% and ≤ 1.49%, respectively. The recovery of the organic acids varied from 97.21 to 99.57%. Organic acids were determined in 44 samples of Brassica rapa var. rapa L. protected under the PGI “Grelos de Galicia” in the two plant status (turnip greens and turnip tops). The results showed that all of the samples presented a profile composed of at least four organic acids: citric, malic, oxalic, and ascorbic acids. However, these organic acids were present in different concentrations in turnip greens and turnip tops.

Keywords

References

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Article

Anti-Proliferative Effects in Human Breast Cancer MDA. MCF-7 Cells & Human Breast Epithelial MCF-10a Cells and Western Blot Analysis from Adlay (Coix Lacryma-Jobi L.) Varieties Phenolic Extracts

1School of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, Jiangsu PR, China

2Department of Food Science, Cornell University, Ithaca, NY


Journal of Food and Nutrition Research. 2014, 2(11), 792-799
DOI: 10.12691/jfnr-2-11-6
Copyright © 2014 Science and Education Publishing

Cite this paper:
Lifeng Wang, Huihui Xie, Yumei Wang, Ruihai Liu, Xingrong Ju. Anti-Proliferative Effects in Human Breast Cancer MDA. MCF-7 Cells & Human Breast Epithelial MCF-10a Cells and Western Blot Analysis from Adlay (Coix Lacryma-Jobi L.) Varieties Phenolic Extracts. Journal of Food and Nutrition Research. 2014; 2(11):792-799. doi: 10.12691/jfnr-2-11-6.

Correspondence to: Lifeng  Wang, School of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, Jiangsu PR, China. Email: wanglifeng_8@163.com

Abstract

Analyzed the anti-proliferative activities (EC50 values) and cytotoxicities (CC50 values) of adlay extracts with adlay (Coix lacryma-jobi L.) varieties grains, Guizhou Heigu (hard hull, black), Liaoning 5 (soft hull, black) and Longyi 1 (hard hull, brown) to be the materials, normal human MCF-10a breast epithelial cells, human MDA breast cancer cells and human MCF-7 breast cancer cells to be the research subjects. After being processed by free or bound phenolic extracts of adlay, detected the expression levels of proteins such as PCNA, p21 and CDK4, which were related to cell proliferation and cell cycle in cells, by using western-blot test to preliminary obtain the mechanism of inhibition of tumor cells proliferation of adlay extracts. The results showed that the adlay extracts by different varieties had obvious anti-proliferative effects to tumor cells, and adlay samples themselves had no cytotoxicity in the tested experimental concentration range. Besides, adlay extracts inhibited the proliferation of HepG2 cells by regulating the cell cycle mediated by DNA polymerase adjustment factors such as PCNA and p21.

Keywords

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