Welcome to Journal of Food and Nutrition Research

Journal of Food and Nutrition Research is a peer-reviewed, open access journal that provides rapid publication of articles in all areas of food and nutrition. The goal of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of food and nutrition.

ISSN (Print): 2333-1119

ISSN (Online): 2333-1240

Editor-in-Chief: Prabhat Kumar Mandal

Website: http://www.sciepub.com/journal/JFNR

   

Article

Regeneration, Nutritional Values, and Antioxidants in Excised Adventitious Shoot of Radish Affected by Dark Treatment

1Department of Horticulture, Catholic University of Daegu, Gyeongsan, Gyeongsangbuk-do 712-702, Republic of Korea

2Department of Development in Oriental Medicine Resources, Sunchon National University, Suncheon, Jeollanam-do, Republic of Korea


Journal of Food and Nutrition Research. 2015, 3(6), 365-370
doi: 10.12691/jfnr-3-6-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Hyun-Sug Choi, Se Ji Jang, Hye Ji Park, Young Beom Yun, Yong In Kuk. Regeneration, Nutritional Values, and Antioxidants in Excised Adventitious Shoot of Radish Affected by Dark Treatment. Journal of Food and Nutrition Research. 2015; 3(6):365-370. doi: 10.12691/jfnr-3-6-2.

Correspondence to: Yong  In Kuk, Department of Development in Oriental Medicine Resources, Sunchon National University, Suncheon, Jeollanam-do, Republic of Korea. Email: yikuk@sunchon.ac.kr

Abstract

The study was to evaluate regeneration, mineral nutrients, and antioxidative activities of adventitious shoot in tuberous root radish (Raphanus sativus L.) grown under light and dark conditions in a controlled growth chamber. Small pieces of top of root radish, including adventitious shoots, were detached from mother radish roots (1st cut) and grown for 22 days (22 DAC1), which then re-cut (2nd cut) and re-grown for 23 days (23 DAC2). Fresh weight was heavier on plants grown under the dark condition (6.2 g) at 22 DAC1 compared to the light condition (5.2 g) but was not significantly different between the treatment conditions at 23 DAC2. Shoot length was significantly extended by the dark condition during the experimental period. Leaf concentrations of Ca, Mg, Na, and Mn were significantly increased by the light condition, with high leaf concentrations of Fe, Zn, and Cu observed on the dark condition. Proline of total amino acids was highly increased by the light condition (126.8 mg g-1) compared to dark condition (16.3 mg g-1), but other total amino acid concentrations were varied between the treatment conditions. Fructose and sucrose were increased by dark and light conditions, respectively. Dark condition reduced scavenging activity (9.7%), total phenolic (12.2 mg g-1) and flavonoid compounds (0.05 mg g-1) compared to the light condition (scavenging activity; 49.3%, total phenolic 43.8 mg g-1 and flavonoid compounds; 0.21 mg g-1) at 22 DAC1, which was also observed at 23 DAC2. Excised adventitious shoot of radish grown under the dark condition could be useful propagation technique for convenient consumption at all times and the physicochemical values to some degree.

Keywords

References

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Article

Molecular Mechanism of Action for the Geranyl Flavonoid to Counter Dyslipidemia in Diabetic Milieu

1Laboratory of Ethnopharmacology, Institute for Nanobiomedical Technology and Membrane Biology, Regenerative Medicine Research Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan 610041, China

2College of Mathematics, Sichuan University, Chengdu 610064, P.R. China

3College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, Sichuan 610065, PR China

4School of Basic Clinical Medical, Beijing University of Traditional Chinese Medicine, Beijing 100029, China


Journal of Food and Nutrition Research. 2015, 3(6), 371-378
doi: 10.12691/jfnr-3-6-3
Copyright © 2015 Science and Education Publishing

Cite this paper:
Hai Niu, Ke Li, Limei Ma, Weihong Gong, Wen Huang. Molecular Mechanism of Action for the Geranyl Flavonoid to Counter Dyslipidemia in Diabetic Milieu. Journal of Food and Nutrition Research. 2015; 3(6):371-378. doi: 10.12691/jfnr-3-6-3.

Correspondence to: Wen  Huang, Laboratory of Ethnopharmacology, Institute for Nanobiomedical Technology and Membrane Biology, Regenerative Medicine Research Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan 610041, China. Email: niuhai@scu.edu.cn; huangwen@scu.edu.cn

Abstract

This work was to examine the effect of 3’-methyl-4’, 7-dihydroxyflavanone, a geranyl flavonoid (GF), on hepatocellular AMPK activity and lipid levels in HepG2 cells and diabetic mice, and identify molecular mechanism of the GF action on remedying dyslipidemia. The AMPK activation and lipid-lowering effect of the GF in diabetic mice and in HepG2 cells paralleled observations. The GF activated AMPK in HepG2 cells treated with high glucose, and enhance phosphorylation of ACC1 and ACC2, two isoforms of ACC, resulting in decrease in ACC activity and hepatic lipids. As demonstrated in cells overexpressing a dominant-negative AMPK mutant, the effect of GF was shown to be mediated by the activation of AMPK. The AMPK was activated relatively rapidly by GF and well before any potential change in adenosine triphosphate (ATP) level was detected. Thus, both in vivo and in vitro inhibition of AMPK, activation of ACC, and hepatocellular lipid accumulation caused by sustained high glucose levels was effectively counteracted by activating AMPK with treatment of GF. It can be conclude that GF lower lipids both in vivo and in vitro by activating AMPK and inactivating ACC, and consequently down-regulating fatty acid synthesis. The work provides a strong evidence for GF as a new therapeutic agent to definitively remedy dyslipidemia in diabetic milieu.

Keywords

References

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Article

Gastrointestinal Tissue Distribution of β-Conglycinin in Pigs at Different Growth Stages

1Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal nutrition and feed science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, P.R. China

2Changchun Property Management School, Changchun, P.R.China

3Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China


Journal of Food and Nutrition Research. 2015, 3(6), 379-383
doi: 10.12691/jfnr-3-6-4
Copyright © 2015 Science and Education Publishing

Cite this paper:
Yuan Zhao, Bing Zhang, Guixin Qin, Tao Wang, Nan Bao, Xiaodong Zhang. Gastrointestinal Tissue Distribution of β-Conglycinin in Pigs at Different Growth Stages. Journal of Food and Nutrition Research. 2015; 3(6):379-383. doi: 10.12691/jfnr-3-6-4.

Correspondence to: Guixin  Qin, Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal nutrition and feed science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, P.R. China. Email: qgx@jlau.edu.cn

Abstract

Soybean allergens may cross the gastrointestinal tissue and induce allergy, and their gastrointestinal tissue distribution could provide the basis of the allergic mechanism to some extent. But the relevant literatures for β-conglycinin are scarce. In the current study, the variation of β-conglycinin in gastrointestinal tissue in vivo was investigated using pigs as animal model in order to compare the distribution differences of β-conglycinin between the growth stages. Fifteen General No.1 barrows weaned on the 28th day were selected to carry out the animal experiments of three ages including weanling, growing and finishing stage. Pigs were fed diets with 4% purified β-conglycinin in experimental periods. The immunohistochemistry method was performed to detect the gastrointestinal distribution of β-conglycinin. The results indicated that there was a significant difference on the gastrointestinal mucosal distribution of β-conglycinin between pigs of different ages. The β-conglycinin went up from stomach to dedodenum, then slightly dropped until proximal-jejunum, and kept to rise until ileum for piglets (P<0.001). It from stomach to distal-jejunum increased slowly, but fell sharply for growers and finishers (P<0.001). The highest content of β-conglycinin was in the deodenum and ileum for piglets, and in distal-jejunum for growers and finishers (P<0.05). The β-conglycinin in intestinal villi and mucosa had similar distribution variation. The distribution of β-conglycinin in intestinal villi and crypt were not affected by growth phase.

Keywords

References

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