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Article

Anti-Proliferative Effects in Human Breast Cancer MDA. MCF-7 Cells & Human Breast Epithelial MCF-10a Cells and Western Blot Analysis from Adlay (Coix Lacryma-Jobi L.) Varieties Phenolic Extracts

1School of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, Jiangsu PR, China

2Department of Food Science, Cornell University, Ithaca, NY


Journal of Food and Nutrition Research. 2014, 2(11), 792-799
DOI: 10.12691/jfnr-2-11-6
Copyright © 2014 Science and Education Publishing

Cite this paper:
Lifeng Wang, Huihui Xie, Yumei Wang, Ruihai Liu, Xingrong Ju. Anti-Proliferative Effects in Human Breast Cancer MDA. MCF-7 Cells & Human Breast Epithelial MCF-10a Cells and Western Blot Analysis from Adlay (Coix Lacryma-Jobi L.) Varieties Phenolic Extracts. Journal of Food and Nutrition Research. 2014; 2(11):792-799. doi: 10.12691/jfnr-2-11-6.

Correspondence to: Lifeng  Wang, School of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, Jiangsu PR, China. Email: wanglifeng_8@163.com

Abstract

Analyzed the anti-proliferative activities (EC50 values) and cytotoxicities (CC50 values) of adlay extracts with adlay (Coix lacryma-jobi L.) varieties grains, Guizhou Heigu (hard hull, black), Liaoning 5 (soft hull, black) and Longyi 1 (hard hull, brown) to be the materials, normal human MCF-10a breast epithelial cells, human MDA breast cancer cells and human MCF-7 breast cancer cells to be the research subjects. After being processed by free or bound phenolic extracts of adlay, detected the expression levels of proteins such as PCNA, p21 and CDK4, which were related to cell proliferation and cell cycle in cells, by using western-blot test to preliminary obtain the mechanism of inhibition of tumor cells proliferation of adlay extracts. The results showed that the adlay extracts by different varieties had obvious anti-proliferative effects to tumor cells, and adlay samples themselves had no cytotoxicity in the tested experimental concentration range. Besides, adlay extracts inhibited the proliferation of HepG2 cells by regulating the cell cycle mediated by DNA polymerase adjustment factors such as PCNA and p21.

Keywords

References

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Article

Multi-Generations Assessment of Dietary Nucleotides Consumption in Maternal Rats

1Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, PR China

2Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing, PR China


Journal of Food and Nutrition Research. 2014, 2(11), 800-805
DOI: 10.12691/jfnr-2-11-7
Copyright © 2014 Science and Education Publishing

Cite this paper:
Xu Meihong, Yi Ma Zhao, Linlin Xu, Yajun Xu, Yong Li. Multi-Generations Assessment of Dietary Nucleotides Consumption in Maternal Rats. Journal of Food and Nutrition Research. 2014; 2(11):800-805. doi: 10.12691/jfnr-2-11-7.

Correspondence to: Yong  Li, Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, PR China. Email: liyongbmu@163.com

Abstract

This study investigated multi-generational reproductive and developmental toxicity of dietary nucleotides (NTs) during lactation in rats with the purpose of augmenting currently available safety data. Weanling rats (F0) were fed NTs at doses of 0.01%, 0.04%, 0.16%, 0.64%, and 1.28% (wt/wt for 90 days and then mated in a 1:1 pattern. The F1 generation rats were fed NTs for 90 days after weaning. The process was repeated until F3 rats were born. We measured the physical and neural development landmarks during lactation in the F3 generation and observed the signs of maternal rats during the gestation, parturition, and lactation periods. No significant differences in either physical or neural developmental landmarks were observed between the control and NT groups. No clinically adverse effects were found in the maternal rats at any point in gestation, parturition, or lactation. There were no alterations in the indicators of parturition. No significant differences were observed between controls and NT-treated rats fed four kinds of nucleotides in F0 milk on PND 21. In conclusion, NTs in doses of up to 1.28% did not cause any adverse effects in either the maternal or filial individuals.

Keywords

References

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Article

Lifetime Assessment of Dietary Nucleotides Consumption in Sprague-Dawley Rats

1Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, PR China

2Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing, PR China


Journal of Food and Nutrition Research. 2014, 2(11), 806-813
DOI: 10.12691/jfnr-2-11-8
Copyright © 2014 Science and Education Publishing

Cite this paper:
Meihong Xu, Rui Liang, Qianying Guo, Shuangjia Wang, Ming Zhao, Zhaofeng Zhang, Junbo Wang, Yong Li. Lifetime Assessment of Dietary Nucleotides Consumption in Sprague-Dawley Rats. Journal of Food and Nutrition Research. 2014; 2(11):806-813. doi: 10.12691/jfnr-2-11-8.

Correspondence to: Yong  Li, Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, PR China. Email: liyongbmu@163.com

Abstract

To assess the long-term toxicity and carcinogenicity of dietary nucleotides (NTs), Sprague-Dawley rats were administered the NTs for life-through with the dose of 0, 0.01%, 0.04%, 0.16% and 0.64%. With the exception of several groups, the results showed no significant differences in weight gain, clinical symptoms data, blood indicators, and Non-neoplastic lesions frequencies. NTs have dramatically increased the food consumption of both sexes (P<0.05). Meanwhile, NTs decreased spontaneous tumor incidence of both sexes, notably the male rats (P<0.05). The occurrence of all malignant and mammary tumors in NTs-treated females was lower than those in controls (P<0.05). In comparison with the control group, the incidence of death from malignant and systemic tumors were obviously decreased in the NTs-treated groups (P<0.01). Moreover, our results showed that a higher percentage of rats in the NTs-treated groups died of non-neoplastic lesions compared with control. In conclusion, dietary nucleotides are not toxic or carcinogenic in rats up to 0.64% for all the life, and 0.64% was an unobservable effect dose.

Keywords

References

[1]  Fontana L, Moreira E, Torres MI, et al. Dietary nucleotides correct plasma and liver microsomal fatty acid alterations in rats with liver cirrhosis induced by oral intake of thioacetamide. J Hepatol. 1998; 28 (4): 662-9.
 
[2]  Jyonouchi H, Sun S, Winship T, et al. Dietary ribonucleotides increase antigen-specific type 1 T-helper cells in the regional draining lymph nodes in young BALB/cJ mice. Nutrition. 2003; 19 (1): 41-6.
 
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[4]  Frankic T, Pajk T, Rezar V, et al. The role of dietary nucleotides in reduction of DNA damage induced by T-2 toxin and deoxynivalenol in chicken leukocytes. Food Chem Toxicol. 2006; 44 (11): 1838-44.
 
[5]  Tahmasebi KA, Keyvanshokooh S, Nematollahi A, et al. Dietary administration of nucleotides to enhance growth, humoral immune responses, and disease resistance of the rainbow trout (Oncorhynchus mykiss) fingerlings. Fish Shellfish Immunol. 2011; 30 (1): 189-93.
 
Show More References
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Article

Effect of Fat Content on Water Sorption Properties of Biscuits Studied by Nuclear Magnetic Resonance

1Jiangnan University, Wuxi, P.R. China

2Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Wuxi, P.R. China

3Rochester Institute of Technology, 78 Lomb Memorial Drive, Rochester, NY 14623, USA


Journal of Food and Nutrition Research. 2014, 2(11), 814-818
DOI: 10.12691/jfnr-2-11-9
Copyright © 2014 Science and Education Publishing

Cite this paper:
Fa-yi Hao, Li-xin Lu, Chang-feng Ge. Effect of Fat Content on Water Sorption Properties of Biscuits Studied by Nuclear Magnetic Resonance. Journal of Food and Nutrition Research. 2014; 2(11):814-818. doi: 10.12691/jfnr-2-11-9.

Correspondence to: Li-xin  Lu, Jiangnan University, Wuxi, P.R. China. Email: lulx@jiangnan.edu.cn

Abstract

Moisture sorption isotherm is a well established method to characterize water sorption properties and behavior of food materials. However, this approach doesn’t adequately reflect the molecular mobility that taking place during water sorption process. Nuclear magnetic resonance (NMR) can provide information about the water mobility and molecular interactions between water and food components. The biscuits with different fat addition were studied using water sorption isotherm and 1H low-field NMR at and water activity ranging from 0.2 to 0.90, the changes in equilibrium moisture content, transverse relaxation time(T2) and proton intensity of biscuits were defined. The T2 were measured with Carr-Purcell-Meiboom-Gill (CPMG) sequences. It was demonstrated that fat content of biscuits influenced directly the equilibrium moisture content and water status. One or two water populations were observed as the water activity increased, each of which had a distinct relaxation time T2 or molecular mobility. The relaxation time manifested that with the increase of fat addition, the water inside the samples became more mobile, and proton intensity indicated that the amount of water uptake decreased with increasing fat addition. The low-field NMR was demonstrated to provide complementary interpretation to that of water sorption isotherm.

Keywords

References

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Article

Analyze the Metabolites in Rats Small Bowel by UPLC-Q-TOF/MS after Oral Administration of Icaritin

1College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, Nanjing, Jiangsu Province, China

2Key Laboratory of New Drug Delivery System of Chinese Material Medica, Jiangsu Provincial Academy of Chinese Medicine, 100# Shizi Road, Nanjing, Jiangsu Province, China


Journal of Food and Nutrition Research. 2014, 2(11), 819-823
DOI: 10.12691/jfnr-2-11-10
Copyright © 2014 Science and Education Publishing

Cite this paper:
Jun Jiang, Li Cui, Xiaobin Jia. Analyze the Metabolites in Rats Small Bowel by UPLC-Q-TOF/MS after Oral Administration of Icaritin. Journal of Food and Nutrition Research. 2014; 2(11):819-823. doi: 10.12691/jfnr-2-11-10.

Correspondence to: Xiaobin  Jia, Key Laboratory of New Drug Delivery System of Chinese Material Medica, Jiangsu Provincial Academy of Chinese Medicine, 100# Shizi Road, Nanjing, Jiangsu Province, China. Email: xiaobinjia_nj@126.com

Abstract

Icaritin (IT) was the main product of intestinal metabolism of main flavonoid compounds in Epimedium. Our previous study of the intestinal metabolites of IT was mainly focused on detecting the metabolites in rat faeces, which did not directly reacted and premeditate the complex environment of small bowel. Therefore, in this paper, the contents of small bowel were collected and analyzed by ultra performance liquid chromatography/quadrupole time of flight mass spectrometry (UPLC-Q-TOF/ MS), and then the main metabolites and metabolic pathways of IT in rats small bowel were explained clearly. Finally, six major metabolites were found in rats small bowel after oral administration of IT, which were m/z 367.1187, 383.1125, 545.1678, 559.1448, 561.1610, and 721.1979, respectively. These six metabolites and their chemical structures were firstly discovered and identified by us. The metabolites m/z 559.1448 and 545.1678 were the combinations of m/z 383.1125 and 369.1308 (IT) linked with one glucuronic acid, respectively. In the intestinal contents, the IT prototype had the highest peak areas, which indicated that IT mainly existed in the form of prototype. Meanwhile, our results further confirmed that the bioavailability of IT might be low, and subsequent research would likely focus on improving the biopharmaceutical properties and enhancing the bioavailability of IT. This study was useful and complementary to our previous findings.

Keywords

References

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Article

Comparison of Five Prescriptions Composed by Coptidis Rhizoma and Evodiae Fructus Based on Separation and Quantification of Eight Active Ingredients and Chemometrics Analysis

1Affiliated Hospital on Integration of Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China

2Key Laboratory of New Drug Delivery System of Chinese Material Medica, Jiangsu Provincial Academy of Chinese Medicine, 100# Shizi Road, Nanjing, Jiangsu Province, China


Journal of Food and Nutrition Research. 2014, 2(11), 824-830
DOI: 10.12691/jfnr-2-11-11
Copyright © 2014 Science and Education Publishing

Cite this paper:
Jun Jiang, Yingjie Wei, Xiaobin Jia. Comparison of Five Prescriptions Composed by Coptidis Rhizoma and Evodiae Fructus Based on Separation and Quantification of Eight Active Ingredients and Chemometrics Analysis. Journal of Food and Nutrition Research. 2014; 2(11):824-830. doi: 10.12691/jfnr-2-11-11.

Correspondence to: Xiaobin  Jia, Affiliated Hospital on Integration of Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China. Email: xiaobinjia_nj@126.com

Abstract

Coptidis rhizoma and Evodiae fructus could be compatible with different proportions, which composed of a series of prescriptions, including Zuojinwan (ZJW), Biantongwan (BTW), Ganlusan (GLS), Zhuyuwan (ZYW), and Fanzuojinwan (FZJW). Eight bioactive compounds, chlorogenic acid (1), jatrorrhizine (2), coptisine (3), palmatin (4), berberine (5), evodin (6), evodiamine (7), and rutaecarpine (8), were the main active ingredients in Coptidis rhizome - Evodiae fructus composition. In this paper, above 8 compounds were determined simultaneously in 105 batches of samples by high performance liquid chromatography tandem diode array detector (HPLC-DAD), in which 23 batches of BTW, 14 batches of ZYW, 23 batches of GLS, 20 batches of FZJW, 25 batches of ZJW, and 5 batches of ZJW preparations (ZJWps) were included. All calibration curves showed good linear regression (r2 > 0.9970), good precision, and accuracy with overall intra and inter-day variations of 0.54-1.58% and 0.54-2.07%, respectively, and the overall recoveries of 98.05-100.86%. Subsequently, the principal component analysis (PCA) was used to evaluate and distinguish ZJW, GLS, ZYW, BTW, FZJW and ZJWps according to the contents of the detected 8 active ingredients. Through the PCA analysis, 5 kinds of prescriptions could be dispersed in different areas, and there was no intersection among them. Unexpectedly, 5 batches of ZJWps deviated from the scatters region belonged to ZJW, which indicated that there were some quality problems in ZJWps. This method could be applied to detect and distinguish Coptidis rhizome-Evodiae fructus prescriptions.

Keywords

References

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Article

Nutritional Evaluation, Characterization and Antioxidant Activity of Radix Isatidis Protein Hydrolysates under Simulated Gastrointestinal Digestion

1College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P.R. China


Journal of Food and Nutrition Research. 2014, 2(11), 831-838
DOI: 10.12691/jfnr-2-11-12
Copyright © 2014 Science and Education Publishing

Cite this paper:
Ping Xiao, Hongzhi Huang, Yayun Chen, Jianwei Chen, Xiang Li, Haitao Shi. Nutritional Evaluation, Characterization and Antioxidant Activity of Radix Isatidis Protein Hydrolysates under Simulated Gastrointestinal Digestion. Journal of Food and Nutrition Research. 2014; 2(11):831-838. doi: 10.12691/jfnr-2-11-12.

Correspondence to: Xiang  Li, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P.R. China. Email: lixiang_8182@163.com

Abstract

In this study, the antioxidant activities of alcalase-treated Radix Isatidis protein hydrolysates (RIPH) were compared during a two-stage gastrointestinal (GI) digestion in vitro. Compared with the blank, the enzymatic breakdown of the GI digests increased their 1,1-diphenyl-2-picrylhydrazyl (DPPH) (1.2-fold increase), hydroxyl (1.9-fold increase), (5.6-fold increase) in the radical scavenging activities and the reducing power (4.0-fold increase). The investigated items of RIPH consisted of the degrees of hydrolysis (DH), the compositions of amino acid, the nutritional parameters and the molecular weight distributions (MWD). The analysis of amino acid compositions of RIPH showed that essential amino acids such as isoleucine, threonine, tryptophan, valine and histidine took a high portion. The final Radix Isatidis protein hydrolysate digests (FRIPHD) contained the free amino acids of about 29.4%. Results from this study showed that RIPH is a good source of natural antioxidants that the formed peptides can resist physiological digestion in GI digestive system.

Keywords

References

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Article

Variation of Polyphenols Composition and Bioactivities of Pomegranate Wines along in Vitro Digestion Process

1Country College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China


Journal of Food and Nutrition Research. 2014, 2(11), 839-845
DOI: 10.12691/jfnr-2-11-13
Copyright © 2014 Science and Education Publishing

Cite this paper:
Li Xuan, Liu Linwei, Humaira Wasila, Gao Zhongmei, Yuan Tian, Zhao Beita. Variation of Polyphenols Composition and Bioactivities of Pomegranate Wines along in Vitro Digestion Process. Journal of Food and Nutrition Research. 2014; 2(11):839-845. doi: 10.12691/jfnr-2-11-13.

Correspondence to: Liu  Linwei, Country College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China. Email: linweiliu8@hotmail.com

Abstract

Six pomegranate wines made from 3 pomegranate varieties were subjected to successive in vitro gastrointestinal digestive system to investigate the effects of pomegranate wine variety and peel addition on the variation of phenols and bioactivities along digestion process. Pomegranate variety contributed more to sugar content, titratable acid level, and alcohol degree, while peel addition mainly accounted for color density and polymer color and phenolics profiles of pomegranate wine. With different wine matrixes and digestive reactions, prime phenolics changed differently along digestion. Different pomegranate wine had different bioactive performances in each digestive stage. Generally, peel wines possessed higher bioactive values than relative aril wines except superoxide anion scavenging ability, and gastric environment were more conducive to DPPH and superoxide anion scavenging, xanthine oxidase inhibiting, but protein denaturation inhibition.

Keywords

References

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Article

Acceptance Survey of GM Food in China

1Zhejiang University of Finance & Economics, Xueyuan street, Hangzhou, China


Journal of Food and Nutrition Research. 2014, 2(11), 846-849
DOI: 10.12691/jfnr-2-11-14
Copyright © 2014 Science and Education Publishing

Cite this paper:
Y. T. Shao, H. J. Cai, G. Chen. Acceptance Survey of GM Food in China. Journal of Food and Nutrition Research. 2014; 2(11):846-849. doi: 10.12691/jfnr-2-11-14.

Correspondence to: G.  Chen, Zhejiang University of Finance & Economics, Xueyuan street, Hangzhou, China. Email: modellover@sina.cn

Abstract

The safety issue of genetically modified (GM) food has been controversial for several decades. China is a major GM food import, production and consumption country which accounts for almost 20% of the world's population. This article analyzes the present situation of China's GM food based on domestic consumer behavior, market behavior and government behavior. A wide range of public polls and markets research were taken to collect data of GM food. The roots of GM food develop and some improvements are also discussed in this study.

Keywords

References

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Article

Hypoglycemic and Hypocholesterolemia Effects of Intragastric Administration of Dried Red Chili Pepper (Capsicum Annum) in Alloxan-Induced Diabetic Male Albino Rats Fed with High-Fat-Diet

1Department of Food Science, Faculty of Agriculture, Cairo University, Giza, Egypt


Journal of Food and Nutrition Research. 2014, 2(11), 850-856
DOI: 10.12691/jfnr-2-11-15
Copyright © 2014 Science and Education Publishing

Cite this paper:
Mona Mohamed Abdel Magied, Nadia Abdel Rahman Salama, Marwa Rashad Ali. Hypoglycemic and Hypocholesterolemia Effects of Intragastric Administration of Dried Red Chili Pepper (Capsicum Annum) in Alloxan-Induced Diabetic Male Albino Rats Fed with High-Fat-Diet. Journal of Food and Nutrition Research. 2014; 2(11):850-856. doi: 10.12691/jfnr-2-11-15.

Correspondence to: Mona  Mohamed Abdel Magied, Department of Food Science, Faculty of Agriculture, Cairo University, Giza, Egypt. Email: mon_nas_prof_cairo@yahoo.com

Abstract

This study investigated the effect of different drying methods, such as solar drying at 45°C and conventional drying at 60°C on the quality of red chili pepper for two varieties Serrano and Fresno. The conventional drying method had the highest capsaicin content (2.74 and 1.28%) for Serrano and Fresno varieties respectively. The effect of dried red chili pepper at 1 and 2% and the pure capsaicin at 0.015% were studied in experiments using male albino rats containing 20% high fat diet (HFD) rendered diabetic with alloxan injection for 4 weeks. The lowest value of blood serum glucose was with G4 “diabetic group fed HFD + 0.015% capsaicin” that recorded 160 mg/dl. Moreover, serum cholesterol as well as serum triglycerides for the diabetic groups G4 and also G6 that “fed HFD + 2% Serrano red dried chili pepper” were significantly low. The HDL concentration for the groups G4 and G6 were significantly higher than the G3 “diabetic rats fed with HFD”. Feeding the groups of diabetic rats with HFD + 1 or 2% dried red chili pepper or 0.015% capsaicin, the LDL and VLDL levels as well as total lipids were significantly low as compared with control diabetic HFD “G3”.

Keywords

References

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