American Journal of Microbiological Research

ISSN (Print): 2328-4129

ISSN (Online): 2328-4137

Website: http://www.sciepub.com/journal/AJMR

Current Issue» Volume 3, Number 2 (2015)

Article

Prevalence and Resistance Profile of Acinetobacter baumannii Clinical Isolates from a Private Hospital in Khartoum, Sudan

1Department of Medical Microbiology Laboratory at RCIH

2Professor of Microbiology, Head of Microbiology Department at Royal Care International Hospital (RCIH) Khartoum, Sudan

3Department of Medical Microbiology Laboratory at Royal Care International Hospital (RCIH), Khartoum, Sudan


American Journal of Microbiological Research. 2015, 3(2), 76-79
DOI: 10.12691/ajmr-3-2-6
Copyright © 2015 Science and Education Publishing

Cite this paper:
Muntasir I. Omer, Samia A. Gumaa, Abdullatif A. Hassan, Khaled H. Idris, Osama A. Ali, Mustafa M. Osman, Mahmmoud S. Saleh, Nagla A. Mohamed, Mustafa M. Khaled. Prevalence and Resistance Profile of Acinetobacter baumannii Clinical Isolates from a Private Hospital in Khartoum, Sudan. American Journal of Microbiological Research. 2015; 3(2):76-79. doi: 10.12691/ajmr-3-2-6.

Correspondence to: Muntasir  I. Omer, Department of Medical Microbiology Laboratory at RCIH. Email: muns_sust@hotmail.com

Abstract

Introduction: Acinetobacter baumannii is an important cause of nosocomial infections worldwide. It is difficult to control, and the infections caused by it are difficult to treat, because it is multidrug resistant. Objectives: This retrospective study was conducted to determine the prevalence and antibiotic resistance pattern of A. baumannii at Royal Care International Hospital, Khartoum, Sudan over a 37 month period. Methodology: Antimicrobial susceptibility testing of the isolates was performed by the disk diffusion method as recommended by Clinical Laboratory and Standards Institute CLSI [1]. Result: Non duplicate 275 A. baumannii were isolated out of a total 2899 pathogenic Gram negative isolates (9.5% prevalence). The most frequently isolated A. baumannii was from ICU patients (72%) followed by inpatients (24%) and outpatients (4%). The greatest number of isolates were recovered from sputum (61%) followed by wound (19%). The Resistance rates were higher than most of the internationally reported levels. Cephalosporins, aminoglycoside, aztreonam, fluoroquinolones and carbapenems are becoming practically ineffective, where the colistin elicited the highest susceptibility levels. Conclusion: This report shows for the first time (to our knowledge) the prevalence and resistance profile of A. baumannii in Sudan. The prevalence will help to conduct better infection control policy, and an update the local antibiogram will improve the knowledge of antimicrobial resistance patterns in our region.

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References

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Article

Isolation and Screening of Indigenous Bambara Groundnut (Vigna Subterranea) Nodulating Bacteria for their Tolerance to Some Environmental Stresses

1Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon

2Laboratory of Soil Microbiology, Biotechnology Centre, University of Yaounde I, Yaounde, Cameroon

3Department of Biological Sciences, University of Ngaoundere, Ngaoundere, Cameroon

4Department of Microbiology, Faculty of Science, University of Yaounde I, Yaounde, Cameroon


American Journal of Microbiological Research. 2015, 3(2), 65-75
DOI: 10.12691/ajmr-3-2-5
Copyright © 2015 Science and Education Publishing

Cite this paper:
Ngo Nkot Laurette, Ngo Bisseck Maxémilienne, Fankem Henri, Adamou Souleymanou, Kamguia Kamdem, Ngakou Albert, Nwaga Dieudonné, Etoa François-Xavier. Isolation and Screening of Indigenous Bambara Groundnut (Vigna Subterranea) Nodulating Bacteria for their Tolerance to Some Environmental Stresses. American Journal of Microbiological Research. 2015; 3(2):65-75. doi: 10.12691/ajmr-3-2-5.

Correspondence to: Ngo  Nkot Laurette, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon. Email: lnkot@yahoo.fr

Abstract

Environmental stresses are important limiting factors for crops production. The aim of this experiment is to isolate Legume Nodulating Bacteria (LNB) obtained from root nodules of bambara groundnut (Vigna subterranea L.) plants and evaluate their performance under some environmental constraints. Samples were collected in Cameroon from three location sites of the Humid-forest zone: Logbessou in the Littoral region; Mfoua in the South and Boga in the Centre region. Nodulation of bambara groundnut was examined in plastic bags and root nodules were collected from seedling. After their isolation, the bacteria were confirmed as LNB by re-nodulating Macroptilium atropurpureum. The morphological, cultural and phenotypic characteristics (utilization of carbon, tolerance to salt, pH, aluminium) of isolates were determined. The results obtained were analyzed statistically by ANOVA using the software SPSS analysis version 11.5. Duncan test was used to measure the difference among the means at a level of p<0.05. A collection of 18 isolates was obtained on Yeast Extract Mannitol Agar medium. Authentication experiments, confirmed that the majority of the isolates (66.67%) were LNB due to their ability to infect the host plant. Bambara groundnut isolates are different morphologically. Dendrogram of the phenotypic characteristics showed that, below the boundary level of 50% average similarity, isolates fell into at least three distinct groups. All isolates showed fast-growing capacity. Most isolates (66.67%) were able to grow in a medium with pH as low as and Al concentration of 50 µM (58.33 %). Some isolates (50%) showed weak growth capacity at 4% NaCl. The bambara groundnut isolates tested were able to use a broad range of carbohydrates as sole source of carbon. The isolates from the present study may be useful to increase the symbiotic nitrogen fixation in legume.

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Article

Isolation and Molecular Identification of New Emergent Candida Lusitaniae Isolated from Sudanese Immunocompromised Patients Infected with Oropharyngeal Candidiasis

1Department of Clinical Laboratory, College of Applied medical sciences, Al Jouf University, Sakaka, Saudi Arabia

2Department of Microbiology, College of Medical laboratory, Sudan University of science and technology, Khartoum, Sudan


American Journal of Microbiological Research. 2015, 3(2), 62-64
DOI: 10.12691/ajmr-3-2-4
Copyright © 2015 Science and Education Publishing

Cite this paper:
Mutaz F. Saad, Amr M. Albasha. Isolation and Molecular Identification of New Emergent Candida Lusitaniae Isolated from Sudanese Immunocompromised Patients Infected with Oropharyngeal Candidiasis. American Journal of Microbiological Research. 2015; 3(2):62-64. doi: 10.12691/ajmr-3-2-4.

Correspondence to: Mutaz  F. Saad, Department of Clinical Laboratory, College of Applied medical sciences, Al Jouf University, Sakaka, Saudi Arabia. Email: mutazsaad74@gmail.com

Abstract

Seventy seven oral swab samples (n=77) were collected in period between august 2007 to may 2008 from hospitalized immunocompromised and HIV patients suspected for Oropharyngeal Candidiasis and admitted in different hospitals in Ed-wiuem state and Khartoum state, Sudan. All samples were inoculated on Sabouraud dextrose agar and identified by colonial morphology, Germ tube test and Vitek2 compact system for biochemical identification and antifungal susceptibility test. Out of 77 oral swab samples collected from immunocompromised and HIV patients, 41 (53.3%) samples showed positive growth of Candida, and 36 (46.7%) samples showed negative growth. The identification showed that out of forty one positive cultures, 32 isolates found as Candida albicans (78%), while nine samples (n=9) appeared as non-Candida albicans (22%) and found as Candida lusitaniae according to GTT and Vitek2 Compact identification. Then DNA was extracted from all non-Candida albicans isolates and DNA sequencing was carried and D1/D2 region were determined using NL1 primer. DNA based identification showed that all nine (n=9) GTT negative isolates were Candida lusitaniae (Anamorh Clavispora lusitaniae). This study documented that there are new emergent species of Candida should be considered when dealing with specimen collected from patients suspected for yeast infections. Our results provide useful information that C. lusitaniae can be isolated as well as other Candida species from immunocompromised patients in Sudan.

Keywords

References

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Article

Detection of Extended Spectrum Beta Lactamase (ESBL) Producing Klebsiella pneumoniae Associated with Tuberculosis Suspected Patients in Basra Governorate, South of Iraq

1College of Nursing / University of Basra


American Journal of Microbiological Research. 2015, 3(2), 59-61
DOI: 10.12691/ajmr-3-2-3
Copyright © 2015 Science and Education Publishing

Cite this paper:
Abdulameer Abdullah Al-Mussawi. Detection of Extended Spectrum Beta Lactamase (ESBL) Producing Klebsiella pneumoniae Associated with Tuberculosis Suspected Patients in Basra Governorate, South of Iraq. American Journal of Microbiological Research. 2015; 3(2):59-61. doi: 10.12691/ajmr-3-2-3.

Correspondence to: Abdulameer  Abdullah Al-Mussawi, College of Nursing / University of Basra. Email: dr_ameer2006@yahoo.com

Abstract

Objective: To investigate extended spectrum β- lactamase (ESBL) producing Klebsiella pneumoniae isolated from sputum of tuberculosis suspected patients in Basra governorate. Methods: A total of 28 (30.4 %) isolates of K. pneumoniae were recovered from 92 sputum clinical specimens at Pulmonary and Respiratory Diseases Center (PRDC) in Basra Governorate, Iraq. All these isolates were tested for ESBL production by using chromogenic media. Results: Of 28 isolates of K. pneumoniae, 6 (21.4%) were positive for ESBL production. Conclusion: This finding demonstrates a high percentage of ESBL producers among clinical isolates of K. pneumoniae. Presence of ESBL producing K. pneumoniae associated with TB patient gives a high risk factor to patients.

Keywords

References

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Article

Isolation and Molecular Characterization of Cellulolytic Bacillus Isolates from Soil and Compost

1Department of Botany and Agric. Biotechnology, Faculty of Agriculture, University of Khartoum, 13314 Shambat, Sudan

2Department of Botany, Faculty of Science, University of Khartoum, Sudan


American Journal of Microbiological Research. 2015, 3(2), 55-58
DOI: 10.12691/ajmr-3-2-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Elhadi A. I. Elkhalil, Fatima Y. Gaffar, Marmar A. El Siddig, Huda A. H. Osman. Isolation and Molecular Characterization of Cellulolytic Bacillus Isolates from Soil and Compost. American Journal of Microbiological Research. 2015; 3(2):55-58. doi: 10.12691/ajmr-3-2-2.

Correspondence to: Elhadi  A. I. Elkhalil, Department of Botany and Agric. Biotechnology, Faculty of Agriculture, University of Khartoum, 13314 Shambat, Sudan. Email: eaikhalil@yahoo.com

Abstract

Fifty five Bacillus isolates were isolated from compost, and alkaline silty clay soil (rhizosphere of potato plant) in Shambat, Khartoum North, Sudan, and screened using morphological tests, biochemical and molecular characterization using 16s rDNA analysis., Screening of cellulase producing isolates was done using carboxyl methyl cellulose (CMC) as a substrate at 25°C. Twenty six isolates were found to be cellulase producers. Among the isolates, four isolates, 9+, 23, 20 and 13 showed high potential in producing extracellular cellulase and had an average cellulase activity of 2.89, 3.12, 3.48 and 3.53 Unit/ml, respectively. Genetic distance between the four isolates with high cellulase activity was determined with RAPD analysis based on OPC-3 primer.

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References

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Article

Molecular Identification 0f 16s Ribosomal RNA Gene of Helicobacter pylori Isolated from Gastric Biopsies in Sudan

1Department of Microbiology, Kassala University, Sudan

2Department of Microbiology, Khartoum University, Sudan

3Virology Department Central lab, Sudan

4Consultant Physician and Gastroenterology, Sudan

5Department of Bioinformatics, Africa City of Technology, Sudan

6Division of Molecular Genetics, Institute of Human Genetics, University of Tübingen, Germany


American Journal of Microbiological Research. 2015, 3(2), 50-54
DOI: 10.12691/ajmr-3-2-1
Copyright © 2015 Science and Education Publishing

Cite this paper:
Mona Mamoun, Elsanousi S. M., Khalid A. Enan, Abdelmounem E. Abdo, Mohamed A. Hassan. Molecular Identification 0f 16s Ribosomal RNA Gene of Helicobacter pylori Isolated from Gastric Biopsies in Sudan. American Journal of Microbiological Research. 2015; 3(2):50-54. doi: 10.12691/ajmr-3-2-1.

Correspondence to: Mona  Mamoun, Department of Microbiology, Kassala University, Sudan. Email: mnmamoun@gmail.com

Abstract

H. pylori are a ubiquitous microorganism infecting up to half of the world’s population. A total of 81 gastric biopsies taken from patients complaining of gastric disorders in Khartoum state, Sudan screened for H.pylori. Eighteen samples (22.2%) yielded positive culture results. The majority of them were males. Also results indicated higher prevalence of H. pylori in patients with gastritis. Further identification performed using PCR targeted a region of 16S ribosomal RNA gene of H. pylori and gene amplified on 12 samples. Six of isolated sequences subjected to BLAST analysis that showed high similarity to GenBank strains of H. pylori.Multiple sequence alignments were performed between isolated 16S rRNA gene sequences and most related H.pylori strains deposited on GenBank. One isolate differed on one base-pair substitution (G-A) from other isolates and selected reference H.pylori strains. Phylogenetic analysis based on 16S rRNA gene sequences reflects that H.pylori could be originated from Africa.

Keywords

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