ISSN (Print): 2328-4129

ISSN (Online): 2328-4137

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Currrent Issue: Volume 4, Number 5, 2016

Article

Synergistic Effect of Biogenic Silver-nanoparticles with β. lactam Cefotaxime against Resistant Staphylococcus arlettae AUMC b-163 Isolated from T3A Pharmaceutical Cleanroom, Assiut, Egypt

1Department of Botany and Microbiology, Faculty of Science, Assiut University, Egypt


American Journal of Microbiological Research. 2016, 4(5), 132-137
doi: 10.12691/ajmr-4-5-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
M. H. A. Hassan, M.A. Ismail, A.M. Moharram, A. Shoreit. Synergistic Effect of Biogenic Silver-nanoparticles with β. lactam Cefotaxime against Resistant Staphylococcus arlettae AUMC b-163 Isolated from T3A Pharmaceutical Cleanroom, Assiut, Egypt. American Journal of Microbiological Research. 2016; 4(5):132-137. doi: 10.12691/ajmr-4-5-1.

Correspondence to: A.  Shoreit, Department of Botany and Microbiology, Faculty of Science, Assiut University, Egypt. Email: ahmedshoreit@yahoo.com

Abstract

The aim of this study was to biosynthesis silver nanoparticles (AgNPs) from Staphylococcus arlettae AUMC b-163 isolated from T3A pharmaceutical company cleanroom, its antimicrobial activity, and the synergistic effect of AgNPs in combination with commonly used antibiotic Cefotaxime sodium against resistant bacteria. The synthesized AgNPs from bacterial were characterized by using UV-VS spectrophotometer analysis, Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM).UV-VS spectrophotometer analysis showed a peak at 420 nm corresponding to the Plasmon absorbance of silver nanoparticles and FTIR analysis showed the potential biomolecule responsible for the reduction of silver. The structural properties of silver nanoparticles were confirmed using XRD technique, while TEM micrographs revealed that the silver nanoparticles are dispersed and aggregated, and mostly having spherical shape within the size range between 8 and 35 nm. The synthesized silver nanoparticles exhibited a varied growth inhibition activity against the tested pathogenic bacteria. A significant increase in area of growth inhibition was observed when a combination of silver nanoparticles and Cefotaxime antibiotics was applied. The current results revealed that the synthesized silver nanoparticles produced by the bacterial strain Staphylococcus arlettae AUMC b-163 is a promising to be used in medical therapy due to their broad spectrum against some pathogenic bacteria, fungi and resistant tested bacteria.

Keywords

References

[1]  Wright, GDs, “Bacterial resistance to antibiotics: enzymatic degradation and modification” Advanced drug delivery reviews, 57 (10), 1451-1470, 2005.
 
[2]  Dar, MA.; Ingle A.; Rai Ms, “Enhanced antimicrobial activity of silver nanoparticles synthesized by Cryphonectria sp. evaluated singly and in combination with antibiotics” Nanomedicine: Nanotechnology, Biology and Medicine, 9 (1), 105-110, 2013.
 
[3]  Fayaz, AM.; Balaji K.; Girilal M.; Yadav R.; Kalaichelvan PT.; Venketesan Rs, “Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria” Nanomedicine: Nanotechnology, Biology and Medicine, 6 (1), 103-109, 2010.
 
[4]  Geoprincy, G.; Saravanan P.; Gandhi NN.; Renganathan Ss, “A novel approach for studying the combined antimicrobial effects of silver nanoparticles and antibiotics through agar over layer method and disk diffusion method” Digest Journal of Nanomaterials and Biostructures, 6 (4), 1557-1565, 2011.
 
[5]  Shahverdi, AR.; Fakhimi A.; Shahverdi HR.; Minaian Ss, “Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics againstStaphylococcus aureus and Escherichia coli Nanomedicine: Nanotechnology, Biology and Medicine, 3 (2), 168-171, 2007.
 
Show More References
[6]  Kalimuthu, K.; Suresh Babu R.; Venkataraman D.; Bilal M.; Gurunathan Ss, “Biosynthesis of silver nanocrystals by Bacillus licheniformis Colloids and Surfaces B: Biointerfaces, 65 (1), 150-153, 2008.
 
[7]  Bhainsa, KC.; D'souza Ss, “Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus Colloids and Surfaces B: Biointerfaces, 47 (2), 160-164, 2006.
 
[8]  Nanda, A.; Saravanan Ms, “Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE” Nanomedicine: Nanotechnology, Biology and Medicine, 5 (4), 452-456, 2009.
 
[9]  Kim, JS.; Kuk E.; Yu KN.; Kim J-H.; Park SJ.; Lee HJ.; Kim SH.; Park YK.; Park YH.; Hwang C-Ys, “Antimicrobial effects of silver nanoparticles”Nanomedicine: Nanotechnology, Biology and Medicine, 3 (1), 95-101, 2007.
 
[10]  Singh, M.; Manikandan S.; Kumaraguru As, “Nanoparticles: A new technology with wide applications” Res J Nanosci Nanotechnol, 1 (1), 1-11, 2011.
 
[11]  Arya, V.; Komal R.; Kaur M.; Goyal A.; Town Ms, “Silver nanoparticles as a potent antimicrobial agent: a review” Pharmacologyonline, 3 118-124, 2011.
 
[12]  Ljungqvist, B.; Reinmüller Bs, “Airborne viable particles and total number of airborne particles: comparative studies of active air sampling” PDA Journal of Pharmaceutical Science and Technology, 54 (2), 112-116, 2000.
 
[13]  White, TJ.; Bruns T.; Lee S.; Taylor Js, “Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics” PCR protocols: a guide to methods and applications, 18 315-322, 1990.
 
[14]  Gurunathan, S.; Kalishwaralal K.; Vaidyanathan R.; Venkataraman D.; Pandian SRK.; Muniyandi J.; Hariharan N.; Eom SHs, “Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli Colloids and Surfaces B: Biointerfaces, 74 (1), 328-335, 2009.
 
[15]  Saifuddin, N.; Wong C.; Yasumira As, “Rapid biosynthesis of silver nanoparticles using culture supernatant of bacteria with microwave irradiation” Journal of Chemistry, 6 (1), 61-70, 2009.
 
[16]  Silambarasan, S.; Jayanthi As, “Biosynthesis of silver nanoparticles using Pseudomonas fluorescens Research Journal of Biotechnology Vol, 8 3, 2013.
 
[17]  Prema, P.; Raju Rs, “Fabrication and characterization of silver nanoparticle and its potential antibacterial activity” Biotechnology and Bioprocess Engineering, 14 (6), 842-847, 2009.
 
[18]  Perez, C.; Pauli M.; Bazerque Ps, “An antibiotic assay by the agar well diffusion method” Acta Biol Med Exp, 15 113-115, 1990.
 
[19]  Li, P.; Li J.; Wu C.; Wu Q.; Li Js, “Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles” Nanotechnology, 16 (9), 1912, 2005.
 
[20]  Davies, RL.; Etris SFs, “The development and functions of silver in water purification and disease control” Catalysis Today, 36 (1), 107-114, 1997.
 
[21]  Narasimha, G.; Praveen B.; Mallikarjuna K.; Deva Prasad Raju Bs, “Mushrooms (Agaricus bisporus) mediated biosynthesis of sliver nanoparticles, characterization and their antimicrobial activity” International Journal of Nano Dimension, 2 29-36, 2011.
 
[22]  Sondi, I.; Salopek-Sondi Bs, “Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria” Journal of colloid and interface science, 275 (1), 177-182, 2004.
 
[23]  Lara, HH.; Ayala-Núnez NV.; Turrent LdCI.; Padilla CRs, “Bactericidal effect of silver nanoparticles against multidrug-resistant bacteria” World Journal of Microbiology and Biotechnology, 26 (4), 615-621, 2010.
 
[24]  Rai, M.; Deshmukh S.; Ingle A.; Gade As, “Silver nanoparticles: the powerful nanoweapon against multidrug‐resistant bacteria” Journal of applied microbiology, 112 (5), 841-852, 2012.
 
[25]  Kollef, MH.; Golan Y.; Micek ST.; Shorr AF.; Restrepo MIs, “Appraising contemporary strategies to combat multidrug resistant gram-negative bacterial infections–proceedings and data from the Gram-Negative Resistance Summit” Clinical infectious diseases, 53 (suppl 2), S33-S55, 2011.
 
[26]  Senapati, S.; Ahmad A.; Khan MI.; Sastry M.; Kumar Rs, “Extracellular biosynthesis of bimetallic Au–Ag alloy nanoparticles” Small, 1 (5), 517-520, 2005.
 
Show Less References