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American Journal of Medical and Biological Research

ISSN (Print): 2328-4080

ISSN (Online): 2328-4099

Website: http://www.sciepub.com/journal/AJMBR

Current Issue» Volume 3, Number 2 (2015)

Article

Effects of Tenascin-C (TNC) Knockdown on Global Genes Expression

1Department of Public Health, Ministry of Health, Riyadh, Saudi Arabia

2Department of Medical Biochemistry, National Research Center, Cairo, Egypt


American Journal of Medical and Biological Research. 2015, 3(2), 62-67
DOI: 10.12691/ajmbr-3-2-3
Copyright © 2015 Science and Education Publishing

Cite this paper:
Ali S. Alharth, Sherien M. El-Daly. Effects of Tenascin-C (TNC) Knockdown on Global Genes Expression. American Journal of Medical and Biological Research. 2015; 3(2):62-67. doi: 10.12691/ajmbr-3-2-3.

Correspondence to: Ali  S. Alharth, Department of Public Health, Ministry of Health, Riyadh, Saudi Arabia. Email: alharth_a@yahoo.com

Abstract

The extracellular matrix protein tenascin-C (TNC) is up-regulated in many cancers including breast cancer. TNC is associated with tumour progression and poor prognosis. The aim of this study was to investigate the effects of TNC knockdown on global gene expression in TNC expressing invasive breast cancer cell lines. Breast cancer cell lines (MDA-MB-231 and MDA-MB-436) were transfected with small interfering RNA (siRNAs) targeting total TNC. cDNA microarray was used to analyse the effects of TNC knockdown on global gene expression at the mRNA level. Microarray analysis following total TNC knockdown revealed significant changes in gene expression: CREBL2, YWHAE and RRAS2 showed down regulation and QKI was specifically up-regulated. In conclusion, the silencing of TNC expression caused significant alteration on global genes expression associated with tumour progression.

Keywords

References

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Article

Tenascin-C (TNC) Promotes Breast Cancer Cell Invasion and Proliferation: Functional Effects of TNC Knockdown in Highly Invasive Breast Cancer Cell Lines

1Department of Public Health, Ministry of Health, Riyadh, Saudi Arabia


American Journal of Medical and Biological Research. 2015, 3(2), 55-61
DOI: 10.12691/ajmbr-3-2-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Ali S Alharth. Tenascin-C (TNC) Promotes Breast Cancer Cell Invasion and Proliferation: Functional Effects of TNC Knockdown in Highly Invasive Breast Cancer Cell Lines. American Journal of Medical and Biological Research. 2015; 3(2):55-61. doi: 10.12691/ajmbr-3-2-2.

Correspondence to: Ali  S Alharth, Department of Public Health, Ministry of Health, Riyadh, Saudi Arabia. Email: alharth_a@yahoo.com

Abstract

The tumour microenvironment plays a crucial role in the development of breast cancer. Tenascin-C (TNC), a matricellular protein and its high molecular weight (MW) isoforms have been shown to be over-expressed in the stroma of breast cancers and are associated with poor prognosis. The aim of this study was to investigate the effects of TNC knockdown in TNC expressing invasive breast cancer cell lines on cancer cell behaviour. Small interfering RNA (siRNA) targeting different exons in TNC (24, 14 and 14-AD1) were designed, synthesised and transfected into the highly invasive MDA-MB-231 breast cancer cell line. The phenotypic alterations caused by TNC knockdown were analysed by Two Dimension (2D) invasion assays and proliferation assays using the mitotic marker Phispho-Histone H3 (pHH3). The siRNA targeted cells showed significant down-regulation of both total TNC (p <0.001) and high MW isoforms (p <0.001) in MDA-MB-231 cells. Moreover, knockdown of total TNC and high MW TNC isoforms significantly decreased both cell invasion (total TNC p<0.001 and TNC-14 p <0.001) and proliferation (total TNC p <0.001 and TNC-AD1 p <0.05). In conclusion, TNC knockdown significantly decreases proliferation and invasion in breast cancer cell lines, confirming its importance in breast cancer progression.

Keywords

References

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Article

Hypertriglyceridemia Induced Acute Pancreatitis in Pregnancy

1MoulayAbdeallah Hospital, Essaouira, Morocco

2Maternite Souissi, Rabat, Morocco


American Journal of Medical and Biological Research. 2015, 3(2), 53-54
DOI: 10.12691/ajmbr-3-2-1
Copyright © 2015 Science and Education Publishing

Cite this paper:
Adil Elghanmi, Ilham Bahra, Fatima Ezzahara Nour, Hicham Seffar. Hypertriglyceridemia Induced Acute Pancreatitis in Pregnancy. American Journal of Medical and Biological Research. 2015; 3(2):53-54. doi: 10.12691/ajmbr-3-2-1.

Correspondence to: Adil  Elghanmi, MoulayAbdeallah Hospital, Essaouira, Morocco. Email: elghanmi_adil@hotmail.com

Abstract

Hypertriglyceridemia induced acute pancreatitis in pregnancy is a rare and life threatening situation.This pathology is associated with high maternal and fetal mortality and morbidity. Case report: A 24 years old Moroccan women Para 1 Gravida 1 was presented to our Emergency Department of Gynecology and Obstetrics of Moulayabdeallah Hospital, Essaouira, Morocco, at 38 weeks of gestation for acute onset of abdominal pain and uterine contractions. Physical exam revealed signs of severe sepsis. Immediate measures of resuscitation were performed. Emergent cesarean delivery and laparotomy revealed chylous ascites in the abdomen. Blood tests showed a high rate of triglyceride. Through this case report, we want to raise awareness of this medical situation that require immediate recognition because any delay can cause catastrophic consequences for both, the mother and the fetus.

Keywords

References

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