International Journal of Environmental Bioremediation & Biodegradation
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International Journal of Environmental Bioremediation & Biodegradation. 2017, 5(2), 49-59
DOI: 10.12691/ijebb-5-2-3
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Biodegradability of Selected¨COil Spill Dispersants Commonly Used in Nigeria

Prince C. Nnadozie1, and Lucky O. Odokuma1

1Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt, Nigeria, P.M.B. 5323 Choba

Pub. Date: July 04, 2017

Cite this paper:
Prince C. Nnadozie and Lucky O. Odokuma. Biodegradability of Selected¨COil Spill Dispersants Commonly Used in Nigeria. International Journal of Environmental Bioremediation & Biodegradation. 2017; 5(2):49-59. doi: 10.12691/ijebb-5-2-3


The study investigates the biodegradation of two oil spill dispersants: ¡®Eco-Remover¡¯ which was obtained from the Nigerian National Petroleum Corporation (NNPC), Port Harcourt and a locally made unapproved ¡®Rigwash,¡¯ obtained from an industrial chemical store at Trans-Amadi, Port Harcourt. The setups were monitored at two weeks interval over 42-days period at room temperature (approximately 30¡ãC) on a static shake-flask system. The physico-chemical parameters of the samples were analyzed using standard chemical methods such as the atomic absorption spectrophotometry, Gas Chromatography (GC-FID) and the titrimetric technique. The result of primary biodegradation rate revealed that the mixture of Eco-Remover, seawater and crude oil (L2) was the highest degraded at 77.1%, followed by Eco-Remover and seawater combination (L1) at 4.39%. K1 (a mixture of seawater and Rigwash) and K2 (a mixture of crude oil, seawater and Rigwash) degraded at 1.22% and 1.19% respectively. The result also indicated that K1 had an adverse effect on crude oil. Analytical Profile Index of bacterial isolates revealed a total of Five genera found growing on the samples. Among them, Bacillus, Pseudomonas and Kocuria were dominant and possess the potential to utilize dispersants and/or crude oil as the only carbon sources. The fungal isolates include: Aspergillus niger (18.0%), Cladosporium carionii (8.20%), Fusarium oxytoca (4.92%), Myxomycete spp. (13.1%), Fusarium spp. (3.28%), Penicillium spp. (3.28%), Phoma spp. (8.20%), Pleurotus pulmorarius (8.20%), Rhizopus spp. (3.28%), Talaromyces flavus (9.84%), Trichoderma longibrachiatum (6.56%), T. polysporum (4.92%) and Verticillium spp. (8.20%). Analysis of variance at confidence limit P ¡Ü 0.05 showed that there was significant difference in the biodegradation of each dispersant or their combination with crude oil. The implication of this study establishes that Eco-Remover is more biodegradable than Rigwash ¡®dispersant¡¯. However, there is need for further studies on their application in the field to confirm the outcome of this laboratory observations.

pollution crude oil dispersants microorganisms biodegradation

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