Quantitative Determination of Milnacipran by Simple Colorimetric Methods

Md. Mubarakunnisa¹, Avula Prameela Rani¹, Seelam Harika¹ and Chandra Bala Sekaran^2,

¹University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjuna Nagar, India

²Department of Biotechnology, Jagarlamudi Kuppuswamy Choudary College, Guntur, India

Cite this paper:
Md. Mubarakunnisa, Avula Prameela Rani, Seelam Harika and Chandra Bala Sekaran. Quantitative Determination of Milnacipran by Simple Colorimetric Methods. Chemical Engineering and Science. 2013; 1(1):1-6. doi: 10.12691/ces-1-1-1

Abstract

In the present study, two sensitive, precise and accurate spectrophotometric methods have been developed for the determination of milnacipran in bulk and capsule formulation. The first method is based on the reaction of milnacipran with ninhydrin in N,N'-dimethylformamide medium at 80°C temperature to form a colored Ruhemann's purple, which exhibits absorption maximum at 575nm. The second method is based on extraction of milnacipran into chloroform as ion-pair with bromothymol blue. The yellow colored ion-pair complex exhibits absorption maximum at 410nm. Beer’s law is obeyed in the concentration ranges 2.5-37.5μg/mL and 2-12μg/mL of milnacipran for methods I and II, respectively. The effect of experimental variables were investigated and optimized. The validation parameters like linearity, sensitivity, accuracy, precision and robustness were checked by following the ICH guidelines. The proposed methods were applied for the analysis of milnacipran in capsule formulation with good results.

Keywords:
milnacipran ninhydrin bromothymol blue Ruhemann's purple ion-pair complex

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