American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: http://www.sciepub.com/journal/ajbr Editor-in-chief: Hari K. Koul
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American Journal of Biomedical Research. 2013, 1(2), 28-34
DOI: 10.12691/ajbr-1-2-2
Open AccessArticle

Subcomponents of Vitamine B Complex Regulate the Growth and Development of Human Brain Derived Cells

K.E. Danielyan,

Pub. Date: March 22, 2013

Cite this paper:
K.E. Danielyan. Subcomponents of Vitamine B Complex Regulate the Growth and Development of Human Brain Derived Cells. American Journal of Biomedical Research. 2013; 1(2):28-34. doi: 10.12691/ajbr-1-2-2

Abstract

The work is focused on the role of single components of Vitamin B complex: pyridoxine, riboflavin, thiamine, and nicotinamide in the processes of growth and development of the human brain derived cells. We, also, have assayed the activity of Xanthine Oxidase in the presence of above mentioned subcomponents to delineate the possible mechanism of their action. Results indicate that the all components of Vitamin B complex might be responsible for cells’ growth, maturation, proliferation. During early period of the cells’ growth the most important components were thiamine and Pyridoxine, initiating cells’ proliferation (number of the cells in one field: 2556, 17±355, 68, 2179,0±223,55, resp) vs control (1562,94±146,45), whereas during the late stages of maturation the most important components responsible for differentiation, were riboflavin and nicotinamide (3774.77± 188.41, 3558.82±152.90 resp. vs control 2905±263.75; p<0.035). In comparison with the all other subcomponents of Vitamin B complex only in pyridoxine containing samples, XO activity was specifically inhibited by allopurinol (percentile of inhibition 160,00±60,00 vs control 31,03±6,92, p<0,05). We have concluded that Pyridoxine might interact with XO and regulate its activity. All components of Vitamin B complex are able to initiate cells development and growth; however, they are supposed to be selectively utilized in time dependent manner to guarantee the highest efficiency of the cells development, maturation and proliferation

Keywords:
subcomponents of Vitamin B human brain neuronal cell culture xanthine oxidase

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References:

[1]  Castro, G.D., Costantini, M.H. and Castro, J.A., Rat ventral prostate xanthine oxidase-mediated metabolism of acetaldehyde to acetyl radical, Hum Exp Toxicol, 28(4) (2009 ) 203-8.
 
[2]  Danielyan, K.E. and Kevorkian, G.A., Xanthine oxidase activity regulates human embryonic brain cells growth, Biopolym. Cell, 27(5) (2011) 350-353.
 
[3]  Geng, M.-Y., Saito, H. and Katsuki, H., Effects of vitamin B6 and its related compounds on survival of cultured brain neurons, Neuroscience Research, 24 (1995) 61-65.
 
[4]  Geng, M.Y., Saito, H. and Katsuki, H., The effects of thiamine and oxythiamine on the survival of cultured brain neurons, Jpn J Pharmacol, 68 (1995) 349-52.
 
[5]  Geng, M.Y., Saito, H. and Katsuki, H., Effects of vitamin B6 and its related compounds on survival of cultured brain neurons, Neurosci Res, 24 (1995) 61-5.
 
[6]  Hille, R. and Nishino, T., Flavoprotein structure and mechanism. 4. Xanthine oxidase and xanthine dehydrogenase, FASEB J, 9 (1995) 995-1003.
 
[7]  Kruger, N.J., The Bradford method for protein quantitation, Methods Mol Biol, 32 (1994) 9-15.
 
[8]  Lucius, R., Mentlein, R. and Sievers, J., Riboflavin-mediated axonal degeneration of postnatal retinal ganglion cells in vitro is related to the formation of free radicals, Free Radic Biol Med, 24(5) (1998) 798-808.
 
[9]  Mattson, M.P. and Ruchlik, B., Cell culture of cryopreserved human fetal cerebral cortical and hippocampal neurons: neuronal development and resposes to trophic factors, Brain Research, 552 (1990) 2004-212.
 
[10]  Mukherjee, S.K., Klaidman, L.K., Yasharel, R. and Adams, J.D.J., Increased brain NAD prevents neuronal apoptosis in vivo, Eur J Pharmacol, 330 (1997) 27-34.
 
[11]  Olson, J.S., Ballou, D.P., Palmer, G. and Massey, V., The mechanism of action of xanthine oxidase, J. Biol. Chem, 249 (1974) 4363-4382.
 
[12]  Pacher, P., Nivorozhkin, A. and Szabó, C., Therapeutic Effects of Xanthine Oxidase Inhibitors: Renaissance Half a Century after the Discovery of Allopurinol, Pharmacological Reviews, 58 (1) (2006) 87-114.
 
[13]  Patt, A., Harken, A.H., Burton, L.K., Rodell, T.C., Piermattei, D., Schorr, W.J., Parker, N.B., Berger, E.M., Horesh, I.R. and Terada, L.S., Xanthine oxidase-derived hydrogen peroxide contributes to ischemia reperfusion-induced edema in gerbil brains, J Clin Investig, 81 (1988) 1556-1562.
 
[14]  Rahman, A., Katzive, L., Stanley, K. and Henshaw, S.K., A Global Review of Laws on Induced Abortion, International Family Planning Perspectives1985-1997, 24 (1998).
 
[15]  Sanfeliu, C., Wright, J.M. and Kim, S.U., Neurotoxicity of isoniazid and its metabolites in cultures of mouse dorsal root ganglion neurons and hybrid neuronal cell line, Neurotoxicology, 20(6) (1999) 935-44.
 
[16]  Sheline, C.T., Zhou, Y. and Bai, S., Light-induced photoreceptor and RPE degeneration involve zinc toxicity and are attenuated by pyruvate, nicotinamide, or cyclic light, Mol Vis, 16 (2010) 2639-52.
 
[17]  Tennant, J.R., Evaluation of the trypan blue techni2que for determination of cell viability, Transplantation, 2 (1964) 685-94.
 
[18]  Theron, A., Anderson, A., Grabow, G. and Meiring, J.L., In vitro and in vivo stimulation of neutrophil migration and lymphocyte transformation by thiamine related to inhibition of the peroxidase/H202/halide system, Clin. exp. Immunol, 44 (1981) 295-303.