World Journal of Chemical Education
ISSN (Print): 2375-1665 ISSN (Online): 2375-1657 Website: http://www.sciepub.com/journal/wjce Editor-in-chief: Prof. V. Jagannadham
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World Journal of Chemical Education. 2016, 4(4), 80-85
DOI: 10.12691/wjce-4-4-3
Open AccessArticle

Bovine Serum Albumin Bioconjugation with FITC

Nadia Barbero1, , Claudia Barolo1 and Guido Viscardi1

1University of Torino, Department of Chemistry and NIS and INSTM Reference Centre, Via Pietro Giuria 7, 10125, Torino, Italy

Pub. Date: August 03, 2016

Cite this paper:
Nadia Barbero, Claudia Barolo and Guido Viscardi. Bovine Serum Albumin Bioconjugation with FITC. World Journal of Chemical Education. 2016; 4(4):80-85. doi: 10.12691/wjce-4-4-3

Abstract

This laboratory provides students a basic approach to bioconjugation experiments, a topic which is not typically covered in didactical laboratories. Students are required to characterize a fluorescent probe as well as a protein and then to perform a bioconjugate experiment. The resulting marked protein is characterized by means of UV-Vis spectroscopy.

Keywords:
bioconjugation bovine serum Albumin FITC UV-Vis spectroscopy

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