World Journal of Chemical Education
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World Journal of Chemical Education. 2016, 4(4), 80-85
DOI: 10.12691/wjce-4-4-3
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Bovine Serum Albumin Bioconjugation with FITC

Nadia Barbero1, , Claudia Barolo1 and Guido Viscardi1

1University of Torino, Department of Chemistry and NIS and INSTM Reference Centre, Via Pietro Giuria 7, 10125, Torino, Italy

Pub. Date: August 03, 2016

Cite this paper:
Nadia Barbero, Claudia Barolo and Guido Viscardi. Bovine Serum Albumin Bioconjugation with FITC. World Journal of Chemical Education. 2016; 4(4):80-85. doi: 10.12691/wjce-4-4-3


This laboratory provides students a basic approach to bioconjugation experiments, a topic which is not typically covered in didactical laboratories. Students are required to characterize a fluorescent probe as well as a protein and then to perform a bioconjugate experiment. The resulting marked protein is characterized by means of UV-Vis spectroscopy.

bioconjugation bovine serum Albumin FITC UV-Vis spectroscopy

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[1]  Hermanson, G. T. Bioconjugate Techniques, 2nd edition, Academic Press, Elsevier, 2008.
[2]  Barbero, N.; Barni, E.; Barolo, C.; Quagliotto, P.; Viscardi, G.; Napione, L.; Pavan, S.; Bussolino, F. A study of the interaction between fluorescein sodium salt and bovine serum albumin by steady-state fluorescence. Dyes Pigments 2009, 80 (3), 307-313.
[3]  Peters T. Serum albumin. Adv. Prot. Chem. 1985, 37, 161-245.
[4]  Carter, D. C.; Ho, J. X. Structure of serum albumin. Adv. Prot. Chem. 1994, 45, 153-203.
[5]  Barbero, N.; Visentin, S.; Viscardi, G. The different kinetic behaviour of two potential photosensitizers for PDT. J. Photochem. Photobiol. A: Chem. 2015, 299, 38-43.
[6]  Williams, K.R.; Adhyaru, B.; Pierce, R.E.; Schulman S.G. The Binding Constant for Complexation of Bilirubin to Bovine Serum Albumin. An Experiment for the Biophysical Chemistry Laboratory. J. Chem. Educ. 2002, 79 (1), 115-116.
[7]  Liu, J. Investigation of Cu(II) Binding to Bovine Serum Albumin by Potentiometry with an Ion Selective Electrode: A Biophysical Chemistry Experiment for the Undergraduate Curriculum. J. Chem. Educ. 2004, 81 (3), 395-397.
[8]  Liang, P.; Adhyaru, B.; Pearson, W.L.; Williams K.R. The Binding Constant of Estradiol to Bovine Serum Albumin. An Upper-Level Experiment Utilizing Tritium-Labeled Estradiol and Liquid Scintillation Counting. J. Chem. Educ. 2006, 83 (2), 294-295.
[9]  Sohl, J. L.; Splittgerber, A. G. The binding of Coomassie brilliant blue to Bovine Serum Albumin: A physical biochemistry experiment. J. Chem. Educ., 1991, 68 (3), 262-264.
[10]  Podhradský, D.; Drobnica, L.; Kristian, P. Reactions of cysteine, its derivatives, glutathione coenzyme A, and dihydrolipoic acid with isothiocyanates. Experientia 1979, 35 (2), 154-155.
[11]  Jobbágy, A.; Király, K. Chemical characterization of fluorescein isothiocyanate-protein conjugates. Biochym. Biophys. Acta 1966, 124 (1), 166-175.
[12] Extinction Coefficients, last accessed date: 2016, May 25th.
[13]  Gill, S. C.; von Hippel, P.H. Calculation of Protein Extinction Coeffients from Amino Acid Sequence Data. Anal. Biochem. 1989, 182 (2), 319-326.
[14] Peptide Property Calculator, last accessed date: 2016, May 25th.
[15]  Pace, C. N.; Vajdos, F.; Fee, L.; Grimsley, G.; Gray, T. How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 1995, 4 (11), 2411-2423.
[16]  Ambrosini, S.; Beyazit, S.; Haupt, K.; Tse Sum Bui, B. Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition. Chem. Commun. 2013, 49 (60), 6746-6748.
[17]  Hadjmohammadi, M. R.; Chaichi, M. J.; Yousefpour, M. Solvatochromism Effect of Different Solvents on UV-Vis Spectra of Flourescein and its Derivatives. Iran. J. Chem. Chem. Eng. 2008, 27 (4), 9-14.
[18]  Barbero, N.; Napione, L.; Quagliotto, P.; Pavan, S.; Barolo, C.; Barni, E.; Bussolino, F.; Viscardi, G. Fluorescence anisotropy analysis of protein–antibody interaction. Dyes Pigments 2009, 83 (2), 225-229.