World Journal of Chemical Education
ISSN (Print): 2375-1665 ISSN (Online): 2375-1657 Website: http://www.sciepub.com/journal/wjce Editor-in-chief: Prof. V. Jagannadham
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World Journal of Chemical Education. 2019, 7(4), 248-253
DOI: 10.12691/wjce-7-4-3
Open AccessArticle

Effects of Shade on Antibacterial Production in Aloe Vera Plants: A Model Course Based Undergraduate Research Experience for First- and Second-Year Chemistry and Biochemistry Students

Lauren Fields1, Whitney R. Craig1, 2, Sally A. Wasileski1 and Amanda L. Wolfe1,

1Department of Chemistry, University of North Carolina Asheville, One University Heights, Asheville, North Carolina, 28804, United States

2Department of Physical Sciences, Lander University, 320 Stanley Avenue, Greenwood, South Carolina, 29649

Pub. Date: December 12, 2019

Cite this paper:
Lauren Fields, Whitney R. Craig, Sally A. Wasileski and Amanda L. Wolfe. Effects of Shade on Antibacterial Production in Aloe Vera Plants: A Model Course Based Undergraduate Research Experience for First- and Second-Year Chemistry and Biochemistry Students. World Journal of Chemical Education. 2019; 7(4):248-253. doi: 10.12691/wjce-7-4-3

Abstract

As a means to promote student engagement in chemistry, this proposed course-based undergraduate research experience (CURE) provides an opportunity for students to research a topic that is particularly prevalent in national public health headlines. Antibiotic resistance is a public health issue of increasing concern as result of the overuse and often misuse of antibiotics. For this reason, it is important to explore a variety of avenues to discover novel antibiotics, including isolating bioactive natural products from plants and other organisms. Organisms such as plants, bacteria, and fungi have long been utilized for their antibacterial properties, but little is known for how the majority of these natural remedies are affected by varying growth conditions. Herein, we propose a method through which students in first- and second-year chemistry or biochemistry laboratory courses can manipulate growth factors of Aloe vera, an antibiotic-producing plant, to probe how those manipulations affect antibiotic production and therefore Aloe vera’s effectiveness as an herbal medicine. Aloe vera plants were grown under varying shade conditions, and their gel was extracted and evaluated for antibiotic activity using both a broth microdilution assay and an agar diffusion assay against Staphylococcus aureus and Escherichia coli. Through this model, students are able to develop critical thinking skills, derive a hypothesis, and use analytical and biochemical techniques to evaluate their hypothesis.

Keywords:
natural product CURE antibacterial activity

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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References:

[1]  Kerr, M. and Yan, F., “Incoporating Course-Based Undergraduate Research Experiences in Chemistry Laboratory Curricula”, J. Chem. Ed., 93 (4), 658-662, January 2016.
 
[2]  Auchincloss, L.; Laursen, S.; Branchaw, J.; Eagan, K.; Graham, M.; Hanauer, D.; Lawrie, G.; McLinn, C.; Pelaez, N.; Rowland, S.; Towns, M.; Trautmann, N.; Varma-Nelson, P.; Weston, T. and Dolan, E., “Assessment of Course-Based Undergraduate Research Experiences: A Meeting Report” , CBE Life Sci. Educ., 13 (1), 29-40, Spring 2014.
 
[3]  Ballen, C.; Blum, J.; Brownell, S.; Hebert, S.; Hewlett, J.; Klein, J.; McDonald, E.; Monti, D.; Nold, S.; Slemmons, K.; Soneral, P. and Cotner, S., “A Call to Develop Course-Based Undergraduate Research Experiences (CUREs) for Nonmajors Courses”, CBE Life Sci. Educ., 16 (2), Summer 2017.
 
[4]  Engelen, D. L. V.; Suljak, S. W.; Hall, J. P. and Holmes, B. E., “Undergraduate Introductory Quantitative Chemistry Laboratory Course: Interdisciplinary Group Projects in Phytoremediation”, J. Chem. Ed., 84 (1), 128, January 2007.
 
[5]  Sella, A., “Rethinking Practical Classes”, Nature Reviews, 1, October 2017.
 
[6]  Munita, J. M. and Arias, C. A, “Mechanisms of Antibiotic Resistance”, Microbiol. Spectr., 4 (2), October 2016.
 
[7]  CDC. Antibiotic Resistance Threats in the USA; 2019.
 
[8]  Martens, E. and Demain, A. L., “The antibiotic resistance crisis, with a focus on the United States”, J. Antibiot. (Tokyo), 70 (5), 520-526, May 2017.
 
[9]  Wright, G. D., “Opportunities for natural products in 21st century antibiotic discovery”, Nat. Prod. Rep., 34 (7), 694-701, July 2017.
 
[10]  Murray, E.; Allen, C.; Handy, T.; Huffine, C.; Craig, W.; Seaton, S. and Wolfe, A., “Development of a Robust and Quantitative High-Throughput Screening Method for Antibiotic Production in Bacterial Libraries”, ACS Omega, 4 (13), 15414-15420, September 2019.
 
[11]  Wikaningtyas, P. and Sukandar, E., “The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens”, Asian Pacific Journal of Tropical Biomedicine, 8 (3), 16-19, January 2016.
 
[12]  Gupta, P. D. and Birdi, T. J., “Development of botanicals to combat antibiotic resistance”, J. Ayurveda Integr. Med., 8 (4), 266-275, October 2017.
 
[13]  Bukhari, S.; Nawaz, H.; Tariq, S. and Muneer, A., “In vitro antimicrobial activity of aloe vera gel on selected urinary pathogens”, Biomedica, 33 (1), 39-42, March 2017.
 
[14]  Jain, S.; Rathod, N.; Nagi, R.; Sur, J.; Laheji, A.; Gupta, N.; Agrawal, P. and Prasad, S., “Antibacterial Effect of Aloe Vera Gel against Oral Pathogens: An In-vitro Study”, J. Clin. Diagn. Res., 10 (11), 41-44, November 2016.
 
[15]  Ibrahim, M. H. and Jaafar, H. Z., “Reduced photoinhibition under low irradiance enhanced Kacip Fatimah (Labisia pumila Benth) secondary metabolites, phenyl alanine lyase and antioxidant activity”, Int. J. Mol. Sci., 13 (5), 5290-5306, April 2012.
 
[16]  Paez, A.; Michael Gebre, G.; Gonzalez, M. E. and Tschaplinski, T. J., “Growth, soluble carbohydrates, and aloin concentration of Aloe vera plants exposed to three irradiance levels”, Environ. Exp. Bot., 44 (2), 133-139, October 2000.
 
[17]  Surjushe, A.; Vasani, R. and Saple, D., “Aloe Vera: A Short Review”, Indian J. Dermatol., 53 (4), 163-166, 2008.
 
[18]  Budai, M.; Varga, A.; Milesz, S.; Tőzsér, J.; Benkő S., “Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages”, Mol. Immunol., 56 (4), 471-479, December 2013.