World Journal of Agricultural Research
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World Journal of Agricultural Research. 2018, 6(4), 122-131
DOI: 10.12691/wjar-6-4-2
Open AccessArticle

A Study on Root Exudation Pattern and Effect of Plant Growth Promoting Fungi during Biotic and Abiotic Stress in Pigeonpea

Aashif Iqubal Khan1, Rishi Ram Bhandari2, , Ambika Pokhrel2 and Ram Nandan Yadav1

1Institute of Agricultural sciences, Banaras Hindu University, Varanasi, U.P. India

2Institute of Agriculture and Animal Science, Tribhuvan University, Paklihawa Campus, Paklihawa, Nepal

Pub. Date: November 20, 2018

Cite this paper:
Aashif Iqubal Khan, Rishi Ram Bhandari, Ambika Pokhrel and Ram Nandan Yadav. A Study on Root Exudation Pattern and Effect of Plant Growth Promoting Fungi during Biotic and Abiotic Stress in Pigeonpea. World Journal of Agricultural Research. 2018; 6(4):122-131. doi: 10.12691/wjar-6-4-2

Abstract

An experiment was conducted to observe the interaction of Fusarium udum and Macrophomina phaseolina with a rhizospheric microbe Pseudomonas [AKC-O11] to see their impact on pigeonpea under biotic and abiotic conditions. Both biotic [Fusarium udum and Macrophomina phaseolina] and abiotic stress (NaCl) were applied and performances of these microbes were evaluated. The strain was used individually and in combination with the stresses and applied as seed bacterization of pigeonpea (Var. MA-3) seeds to see the impact on total phenol content in plant root exudates. The bacterized seeds were grown under invitro conditions and after three days of germination the seedlings were exposed to biotic stress due to challenge of the pathogens [Fusarium udum and Macrophomina phaseolina] and abiotic stress due to irrigation with salt solution of 100 mM. Root exudates were collected at 48 h, 96 h and 144 h after the application of stresses. The collected root exudates were processed for total phenolic content and High Pressure/Performance Liquid Chromatography (HPLC) analysis. It was observed that total phenol content was low in seeds bacterized with Pseudomonas strain but the concentration increased when the plants were challenged with the pathogen particularly Macrophomina phaseolina and NaCl. Similarly, a similar trend was also observed in gallic acid accumulation. The above results indicates that Pseudomonas strain (AKC-O11) have potential to be used as biocontrol agent that can help pigeonpea plants to combat attack of Macrophomina phaseolina and Fusarium udum as well as salinity.

Keywords:
pigeonpea plant growth promoting fungi biotic abiotic total phenolic content

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References:

[1]  Bokhari M.H., Ashraf M., 1990. Pulse Crops of Pakistan. Biological Society Lahore, Pakistan.
 
[2]  Reddy S. J., Virmani S. M., 1981. “Pigeonpea and its climatic environment,” in Proceedings of the International Workshop on Pigeonpeas, Vol. 1. Patancheru, India: ICRISAT, 259-270.
 
[3]  FAO., FAOSTAT Online Database. Version 2010. URL http://faostat.fao.org/ [March 24 2017].
 
[4]  MoAD. 2016. Statistical information on Nepalese agriculture. Government of Nepal. Ministry of Agriculture and Cooperatives. Agribusiness Promotion and Statistics Division, Singha Durbar, Kathmandu, Nepal.
 
[5]  Kannaiyan J., Nene Y.L., Reddy M.V., Ryan G., Raju T. N., 1984. Prevalence of pigeonpea disease and associated crop losses in Asia Africa and America. Trop Pest Manag. 30: 62-71.
 
[6]  Nene Y.L., Shelia V.K., Sharma S.B., 1989. A world list of chickpea and pigeonpea diseases.Legume Pathology Progress Report – 7, ICRISAT Publication.
 
[7]  Butler E.J., 1906. The wilt disease of pigeonpea and pepper. Agric J India 1: 25-26.
 
[8]  Kannaiyan J., Nene Y.L., 1981. Influence of wilt at different growth stages on yield loss in pigeonpea. Trop Pest Manage 27: 141.
 
[9]  Butler E.J., 1918. Fungi and Diseases in Plants. Thacker Spink and Co., Calcutta, India.
 
[10]  Marasas W.F.O., Nelson P.E., Tousson T.A., 1984. "Toxigenic Fusarium Species: Identity and Mycotoxicology". University Park, Pennsylvania: Pennsylvania State University Press.
 
[11]  Salleh B., Strange R.N., 1988. Toxigenicity of some fusaria associated with plant and human diseases in Malaysian Peninsula. Journal of General Microbiology 134: 841-847.
 
[12]  Nema A.G., 1992. Studies on pectinolytic and cellulolytic enzymes produced by Fusarium udum causing wilt of Pigeonpea. Indian j. Forest., 15:353-355.
 
[13]  Pandey R.N., Pawar S.E., Bhatia C.R., 1995. Effect of culture filtrate of Fusairum udum and fusaric acid on wilt susceptible and resistant pigeonpea cultivars. Indian Phytopathol. 48: 444-448.
 
[14]  Thomas C. A., 1949. A wilt-inducing polysaccharide from Fusarium solani f. eumartii. Phytopathology 39: 572-579.
 
[15]  Charmley L.L., Trenholm H.L., Prelusky D.A., Rosenberg A., 1995. Economic losses and decontamination. Natural Toxins 3: 199-203.
 
[16]  Venter S.L., Steyn P.J., 1998. Correlation between fusaric acid production and virulence of isolates of Fusarium oxysporum that causes potato dry rot in South Africa. Potato Res. 41: 289-294.
 
[17]  Ben-Yephet Y., Shtienberg D., 1997. Effects of the host, the pathogen, the environment and their interactions, on Fusarium wilt in carnation. Phytoparasitica 25: 207-216.
 
[18]  Srobar S., 1978. The influence of temperature and pH on the growth of mycelium of the causative agents of Fusarioses in wheat in Slovakia, Czechoslovakia. UVTI (Ustav Vedecko Technickych informaci) Ochrana Rostlin 14: 269-274.
 
[19]  Woltz S.S., Ebgelhard A.W., 1973. Fusarium wilt of chrysanthemum: effect of nitrogen source and lime on disease development. Phytopathol. 63: 155-157.
 
[20]  Woltz S.S., Jones J.P., 1973. Tomato Fusarium wilt control by adjustments in soil fertility. Proceedings of the Florida State Hortic. Soc. 86: 157-159.
 
[21]  Naik M.K., Reddy M.V., Raju T.N., McDonald D., 1997. Wilt incidence in sole and sorghum intercropped pigeonpea at different inoculum densities of Fusarium udum. Indian Phytopath. 50: 337-341.
 
[22]  Podile A.R., Laxmi V.D.V., 1998. Seed Bacterization with Bacillus subtilis AF 1 Increases Phenylalanine Ammonialyase and Reduces the Incidence of Fusarial Wilt in Pigeonpea. Journal of Phytopathology 146: 255-259.
 
[23]  Smith G.S., Carvil O.N., 1977. Field screening of Commercial and Experimental Soybean Cultivars for their Reaction to Macrophomina phaseolina. Plant Disease 81: 363-368.
 
[24]  Holliday P., 1980. Fungus Diseases of Tropical Crops. Dover Publications, Minneola.
 
[25]  Dhingra, O.D., Sinclair J.B., 1973. Location of Macrophomina phaseolina on soybean plants related to cultural characteristics and virulence. Phytopathol. 63: 934-936.
 
[26]  Mihail J.D., 1992. Macrophomina. In: Methods for research on soilborne phytopathogenic fungi. (Eds. L.L. Singleton, J.D. Mihail, C.M. Rush) St.Paul: APS Press 134-136.
 
[27]  Holt J.G., Krieg N.E., Sneath P.H.A., Staley J.T., Williams S.T., 1994. Standard methods for the eamination of water and edn. Bergeyl’s Manual of determinative bacteriology 4: 93-115.
 
[28]  Kumar R.S., Ayyadurai N., Pandiaraja P., Reddy A.V., Venkateswarlu Y., Prakash O., Sakt N., 2005. Characterization of antifungal metabolic produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits. J. Appl. Microbiol. 98: 145-154.
 
[29]  Lugtenberg B.J.J., Bloemberg G,V., 2004. Life in Rhizosphere. In JL Ramos (ed) Pseudomonas: Genomics life style and molecular architecture. New York, USA. Kleuwer /Acadamic, Plenum Publishers. 1: 403-430.
 
[30]  Johri B.N., Rao C.V.S., Goel R., 1997. Fluorescens pseudomonads in plant diseases management. In:Dadarwal KR, editor. Biotechnical approaches in soil microorganism for sustainable crop production. Jodhpur: Scientific Publishers 33: 193-221.
 
[31]  Gao, G., Yin, D., Chen, S., Xia, F., Yang, J., Li, Q., Wang, W., 2012. Effect of Biocontrol Agent Pseudomonas fluorescens 2P24 on Soil Fungal Community in Cucumber Rhizosphere Using T-RFLP and DGGE. PLoS ONE, 7(2), e31806.
 
[32]  Friend J., 1979. Phenolic substances and plant diseases. Phytochemistry 12: 557-558.
 
[33]  Punja Z.K., 1985. The biology, ecology and control of Sclerotium rolfisii. Annu. Rev. Phytopathol. 23: 97-127.
 
[34]  Bestwick C.S., Bennett M.H., and Mansfield J.W., 1995. Hrp Mutant of Pseudomonas syringae pv. Phaseolicola induces cell wall alterations but not membrane damage leading to the hypersensitive reaction in lettuce. Plant Physiol 108: 503-516.
 
[35]  Benhamou N., 1995. Imunocytochemistry of plant defense mechanisms induced upon microbial attack. Microsc. Res. Techniq. 31: 63-78.
 
[36]  Matern U., Girimmig B., Kneusel R.E., 1995. Plant cell wall reinforcement in the disease resistance response: molecular composition and regulation. Can. J. Bot. 73: 511-517.
 
[37]  Nicholson L.R., Hamerschmidt R., 1992. Phenolic Compound and their role in disease resistane. Annu. Rev. Phytopathol. 30: 369-389.
 
[38]  Puuponen-Pimia R., Nohynek L., Meier C., Kahkonen M., Heinonen M., Hopia A., Oksman- Caldentey K.M., 2001. Antimicrobial properties of phenolic compounds from berries. J. Appl. Microbiol. 90: 494-507.
 
[39]  Sousa A., 2006. Phenolics and antimicrobial activity of traditional stoned table olives ‘alcaparra’. Bio. org. Med. Chem. 14: 8533-8538.
 
[40]  Luzzatto T., Golan A., Yishay M., Bilkis I., Ben-Ari J. Yedidia I.,2007. Priming of microbial Phenolics during induced resistance response towards Pectobacterium arotovorum in the ornamental monocot Calla Lily. J. Agric. Food Chem. 85: 273-282.
 
[41]  Marin-Martinnez R., Veloz-Garcia R., Veloz-Rodriguez R., Guzman-Maldonado H., loarca-Pina G., Cardador-Matinez A., Guevara-Olvera L., Miranda-Lopez R., Torres-Pacheco I., Perez C., Herrera-Hernandez G., Villase nor-Ortega F., Gonzalez-Chavira M., Guevara-Gonzalez R.G., 2009. Antimutagenic and antioxidant activities of quebracho phenolics 577 (Schinopsis balansae) recovered from tanney wast waters. Bio. Resour. Technol. 100: 434-439.
 
[42]  Lavania M., Chauhan P.S., Chauhan S.V.S., Singh H.B., Nautiyal C.S., 2006. Induction of Plant defense Enzymes and Phenolics by Treatment with Plant Growth-Promoting Rhizobacteria Serratia marcescens NBRI 1213. Curr. Microbiol. 52: 363-368.
 
[43]  Singh U.P., Sarma B.K., Singh D.P., Bahadur A., 2002. Effect of plant growth promoting rhizobacteria-mediated induction of phenolics in pea (Pisum sativum) after infection with Erysiphe pisi. Curr. Microbiol. 44: 396-400.
 
[44]  Constable C.P., Barbehenn R., 2008. Defensive roles of polyphenol oxidase in plants. In a Schaller edited Induced Plant resistance to herbivory. Springer Science + Media BV. 253-269.
 
[45]  Deborah S.D., Palaniswami A., Vidhyasekaran P., Velazhahan R., 2001. Time-course study of the induction of defense enzymes, Phenolics and lignin in rice in response to infection by pathogen and non-pathogen. J. Plant Dis. Port. 108: 204-216.
 
[46]  Kloepper J.W., Schroth M.N., 1978. Plant growth promoting rhizobacteria on radishes, In: Proceedings of the 4th international conference on plant pathogenic bacteria, Angers, France 1979. 879-882.
 
[47]  Zahir Z.A., Arshad M., Frankenberger W.T.Jr., 2003. Plant growth promoting rhizobacteria: Applications and perspectives in agriculture. Adv. Agron. 81: 97-168.
 
[48]  Asghar H.N., Zahir Z.A., Arshad M., Khalig A., 2002. Plant growth regulating substances in the rhizosphere: microbial production and functions. Adv Agron 62: 146-151.
 
[49]  Chen C, Belanger RR, Benhamou N and Paulit TC. 2000. Defense enzymes induced in cucumber roots by treatment with plant growth promoting rhizobacteria (PGPR). Physiol. Mol. Plant Pathol. 56: 13-23.
 
[50]  Figueiredo M.V.B., Burity H.A., Martinez C.R., Chanway C.P., 2008. Alleviation of water stress effects in common bean (Phaseolus vulgaris L.) by co-inoculation Paenibacillus x Rhizobium tropici. Applied Soil Ecol 40: 182-188.
 
[51]  Gupta A., Gopal M., Tilak K.V., 2000. Mechanism of plant growth promotion by rhizobacteria. Indian J Exp Biol 38: 856-862.
 
[52]  Kloepper J.W., Schoth M.N., Miller T.D., 1980. Effects of rhizosphere colonization by plant growth- promoting rhizobacteria on potato plant development and yield. Phytopathol. 70: 1078-1082.
 
[53]  Silva V.N., Silva L.E.S.F., Figueiredo M.V.B., 2006. Atuac¸a˜o de rizo´bios com rizobacte´rias promotoras de crescimento em plantas na cultura do caupi (Vigna unguiculata L. Walp). Acta Sci Agron 28: 407-412.
 
[54]  Timmusk S., Wanger E.G.H., 1999. The plant growth promoting rhizobacterium Paenibacillus polymyxa induces change in Arabidopsis thaliana gene expression: A possible connection between biotic and abiotic stress responses. Mol. Plant-Microbe Interact. 12: 951-959.
 
[55]  Van Loon L.C., Bakker P.A.H.M., and Pieters C.M.J., 1998. Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopathol. 36: 453-483.
 
[56]  Kloepper J.W., Ryu C-M, Zhang S., 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94:1259-1266.
 
[57]  Van Loon L.C., Glick B.R., 2004. Increased plant fitness by rhizobacteria. In: Ecological studies. Molecular ecotoxicology of plants. Ecological studies. Sandermann, H. (ed), Springer-Verlag, Berlag Heidelberg. 170: 177-205.
 
[58]  Shilev S., Sancho E. D., Gonzalez M. B., 2012. Rhizospheric bacteria alleviate salt-produced stress in sunflower. Journal of Environmental Management 95:S37-S41.
 
[59]  King E.O., Ward M.K., Raney D.E., 1954. Two simple media for demonstration of pyocyanin and fluorescein. J Lab Clin Med 44: 301-307.
 
[60]  Beever, R E, and E G Bollard. 1970. “The Nature of the Stimulation of Fungal Growth by Potato Extract.” J. Gen. Microbiof. Vol. 60. www.microbiologyresearch.org.
 
[61]  Dhingra D., Sinclair J.B., 1995. Basic Plant Pathology Methods. Second Edition: 251-253.
 
[62]  Akthar and Siddiqui Z.A., 2010. Effect of AM Fungi on the Plant growth and Root-Rot Disease of Chickpea. American-Eurasian j. Agric. Environ. Sci. 8: 544-549.
 
[63]  Faujdar S., Oswalt D. L., 1992. Major Diseases of Groundnut. In (Vol. Skill Development Series no. 6, ICRISAT). URL http://www.icrisat.org/what-we-do/learningopportunities/lsu-pdfs/sds.06.pdf [09/1/2012].
 
[64]  Morton D.T., Strouble N.H., 1955. Antagonistic and stimulatory effects of microorganism upon Sclerotium rolfsii. Phytopathology 45: 419-420.
 
[65]  Marley P. S., Hillocks R. J., 2002. Induction of phytoalexins in pigeonpea (Cajanus cajan) inresponse to inoculation with Fusarium udum and other treatments. Pest Management Science. 58: 1068-1072.
 
[66]  Sahni S., Sarma B.K., Singh D.P., Singh H.B., Singh K.P., 2007. Vermicompost enhances performance of plant growth-promoting rhizobacteria in Cicer arietinum rhizosphere against Sclerotium rolfsii. Crop Protection. 27: 369-376.
 
[67]  Sarma B.K., Singh U.P., Singh K.P., 2002. Variability in Indian isolates of Sclerotium rolfsii Mycologia 94: 1051-1058.
 
[68]  Singh A., Sarma B.K., Upadhyay R.S., Singh H.B., 2013. Compatible rhizosphere microbes mediated alleviation of biotic stress in chickpea through enhanced antioxidant and phenylpropanoid activities. Microbiol. Res. 168: 33-40.
 
[69]  Bais H.P., Tiffany L., Weir L.G., Perry, Gilroy S., Jorge M.V., 2006. The Role of Root Exudates in Rhizosphere Interactions with Plants and Other Organisms. Annu. Rev. Plant Biol. 57: 233-66.
 
[70]  Gray E.J., Smith D.L., 2005. Intracellular and extracellular PGPR: commonalities and distinctions in the plant-bacterium signaling processes. Soil Biol. Biochem. 37: 395-410.
 
[71]  Bonkowski, Michael. 2004. “Protozoa and Plant Growth: The Microbial Loop in Soil Revisited.” New Phytologist 162 (3). Wiley/Blackwell (10.1111): 617–31.
 
[72]  Bais H.P., Fall R., Jorge M.V., 2004. Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production. Plant Physiol. 134: 307-319.
 
[73]  Marschner H., 1995. Mineral Nutrition of Higher Plants, Second Edition. London: Academic.
 
[74]  Pillai B.V.S., Swarup S., 2002. Ilucidation of the flavinoid catabolism in Pseudomonas putida PML2 by comparative metabolic profiling. Applied Microbiol. 68: 143-151.
 
[75]  Bertin C., Yang X.H., Weston L.A., 2003. The role of root exudates and allelochemicals in the rhizosphere. Plant Soil 256: 67-83.
 
[76]  Uren N.C., 2000. Types, amounts and possible functions of compounds released into the rhizosphere by soil grown plants. In: Pinton R, Varanini Z, Nannipieri P (eds) The rhizosphere: biochemistry and organic substances at the soil interface. Dekker, New York. 19-40.
 
[77]  Ashraf M., Waheed A., 1990. Screening of local/exotic accessions of lentil (Lens culinaris Medic.) for salt tolerance at two growth stages. Plant and Soil 128: 167-176.
 
[78]  Subbarao G.V., Johansen G., Jana K.M., Rao K., 1990. Effects of the sodium/calcium ratio in modifying salinity response of pigeonpea (Cajanus cajan L.). J. Plant Physiol. 136: 439-443.
 
[79]  Zhu J.K., 2001. Plants salt tolerance. Trends Plant Sci. 6: 66-72.
 
[80]  Srivastava N., Vadez V., Krishnamurthy L., Saxena K.B., Nigam S.N., Rupakula A., 2007. Standardization of a screening technique for salinity tolerance' in groundnut (Arachis hypogaea L.) and pigeonpea (Cajanus cajan L.). Indian J. Crop Sci. 2: 209-214.
 
[81]  Borowitz J.J., Stankie-Dicz M., Lewicka T., Zukowska Z., 1992. Inhibition of fungal cellulase, pectinase and xylanase activity of plant growth promoting fluorescent pseudomonads. Bull OILB/SROP 15: 103-106.
 
[82]  Bapat S., Shah A.K., 2000. Biological control of fusarial wilts of pigeonpea by Bacillus brevis. Can. J. Microbiol. 46: 125-32.
 
[83]  Siddiqui Z.A., Mahmood I., 1995. Biological control of Fusarium udum and Heterodera cajani can by Bacillus subtilis, Brandyrhizobium joponicum and Glomus fasciculantum on pigeonpea. Fundam. Appl. Nematol. 18: 559-5.
 
[84]  Matta A., 1969. Accumulation of phenols in tomato plants infected by deferent activities and the consequence of stress induced resistance to Fusarium wilt of tomato. Phytopathology 59: 512-513.
 
[85]  Van Peer R., Niemann, G.J., Schippers, B., 1991. Induced resistance and phytoalexin accumulation in biological control of Fusarium wilt of carnation by Pseudomonas sp. Strain WCS417r. Phytopathology 81, 728-734.
 
[86]  Wei G., Kloepper J.W., Tuzun S., 1991. Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth promoting rhizobacteria. Phytopathology 81: 1508-1512.
 
[87]  Chanwitheesuk A., Teerawutgulrag A., Rakariyatham N., 2005. Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food Chem 92: 491-497.