World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: http://www.sciepub.com/journal/wjar Editor-in-chief: Rener Luciano de Souza Ferraz
Open Access
Journal Browser
Go
World Journal of Agricultural Research. 2018, 6(1), 5-9
DOI: 10.12691/wjar-6-1-2
Open AccessArticle

Evaluation the Toxicity of Honey Bee Venom on Achroia grisella Developmental Stages

Montasir O. Mahgoub1, Wei H. Lau2, Dzolkhifli Bin Omar2 and Ahmed M. El Naim3,

1Department of Plant Protection, Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied, Sudan

2Department of Plant Protection, Faculty of Agriculture, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

3Department of Crop Sciences, Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied, Sudan

Pub. Date: January 05, 2018

Cite this paper:
Montasir O. Mahgoub, Wei H. Lau, Dzolkhifli Bin Omar and Ahmed M. El Naim. Evaluation the Toxicity of Honey Bee Venom on Achroia grisella Developmental Stages. World Journal of Agricultural Research. 2018; 6(1):5-9. doi: 10.12691/wjar-6-1-2

Abstract

The common control method used to control the lesser wax moth A. grisella was fumigation with toxic gases; however, many insect pests of honey bees have developed resistance to the conventional control methods. This study aimed to study the toxicity of crude bee venom on developmental stages of A. grisella as safer alternative and replacement of these chemicals. The bee venom was collected by placing the electric bee venom collector device at the entrance of the beehive. Newly deposited eggs of A. grisella were assayed to evaluate the crude honey bee venom effect on the viability of eggs. Dried crude honey bee venom was diluted with pure acetone to concentrations of 50, 25, 12.5 and 6.25 µg/µl. Egg hatchability was significantly (p<0.05) affected by the treatment. The corrected mortality of the treated eggs was 50.54% in the higher concentration of 50µg/µl with average unhatched eggs of 17.5 eggs per total of 25 eggs with the median lethal concentration (LC50) of 52.89 µg /µl. The topical application of crude honey bee venom was applied on 3rd instar larvae with concentrations of 0, 6.25, 12.5, 25, 50 µg. The calculated mortality percentages for all treatments were 8% at the lower concentration and 52% at the high concentration. The calculated lethal median concentration LC50 was 38.27 µg /µl.

Keywords:
bee venom toxicity wax moth

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Figures

Figure of 5

References:

[1]  Lodesani, M., and Costa, M. Limits of chemotherapy in beekeeping: development of resistance and the problem of residues. Journal of Bee World, 86(4), 102-109, 2005.
 
[2]  Lu, C. S., Warchol, K. M. and Callahan, R. A. “In situ replication of honey bee colony collapse disorder”. Bulletin of insectology, 65(1): 99-106, 2012.
 
[3]  Manzoli, M., Gobbi, N., and Palma, M. “Insects as biological models to assay spider and scorpion venom toxicity”. Journal of Venomous Animals and Toxins Including Tropical Diseases, 9(2), 174-185, 2003.
 
[4]  Kirschbaum, J. “Potential implication of genetic engineering and other biotechnologies to insect control”. Annual Review of Entomology, 30(1), 51-70, 1985.
 
[5]  Peiren, N.,de Graaf, D. C.,Vanrobaeys, F., Danneels, E. L., Devreese, B., Van Beeumen, J.,and Jacobs, F. J. “Proteomic analysis of the honey bee worker venom gland focusing on the mechanisms of protection against tissue damage”. Toxicon, 52(1), 72-83. 2008.
 
[6]  Chen, Y. N.,Li, K. C.,Li, Z.,Shang, G. W.,Liu, D.,Lu, Z.,Zhang, J. W.,Ji, Y. H.,Gao, G. D.,and Chen, J. “Effects of bee venom peptidergic components on rat pain-related behaviors and inflammation”. Neuroscience, 138(2), 631-640, 2006.
 
[7]  Lima, P. R.,and Brochetto B., M. R. “Hymenoptera venom review focusing on Apis mellifera”. Journal of Venomous Animals and Toxins Including Tropical Diseases, 9(2), 149-162, 2003.
 
[8]  Bogdanov, S. “Bee Venom: Composition, Health, Medicine: A Review”. Journal of Peptides, (1), 1-20, 2012.
 
[9]  Zhou, J., Zhao, J., Zhang, S., Shen, J., Qi, Y., Xue, X., Li, Y.,Wu, L.,Zhang, J., Chen, F.,and Chen, L. “Quantification of melittin and apamin in bee venom lyophilized powder from Apis mellifera by liquid chromatography diode array detector tandem mass spectrometry”. Journal of Analytical Biochemistry, 404(2), 171-8, 2010.
 
[10]  Rybak-Chmielewska, H., and Szczêsna, T. “HPLC study of chemical composition of honeybee (Apis mellifera L.) venom”. Journal of Apicultural Science, 48(2), 103-109, 2004.
 
[11]  Moyses, E. W.,and Gfeller, F. J. Topical application as a method for comparing the effectiveness of insecticides against cat flea (Siphonaptera: Pulicidae). Journal of Medical Entomology, 38(2), 193-196, 2001.
 
[12]  Rogers, M., Cole, T., Ramaswamy, S.,and Potter, D. “Behavioral changes in Japanese beetle and masked chafer grubs (Coleoptera: Scarabaeidae) after parasitism by Tiphiid wasps (Hymenoptera: Tiphiidae)”. Journal of Environmental Entomology, 32(3), 618-625, 2003.
 
[13]  Ross, D.C., Crim, J.W., Brown, M.R., Herzog, G.A., lea, A.O. “Toxic and antifeeding actions of melittin in the corn earworm, Heliothis zea (Boddie): Comparisons to bee venom and insecticides chlorpyriphos and cyromazine”. Toxicon, 25(3): 307-313. 1987.
 
[14]  West, E. J.,and Briggs, J. D. “In vitro toxin production by the fungus Beauveria bassiana and Bioassay in greater wax moth larvae”. Journal of Economic Entomology, 61(3), 684-687, 1968.
 
[15]  Lowy, P. H., Sarmiento, L.,and Mitchell, H. K. “Polypeptides minimine and melittin from bee venom: Effects on Drosophila”. Archives of Biochemistry and Biophysics, 145(1), 338-343, 1971.
 
[16]  Hider, R. C. “Honeybee venom: A rich source of pharmacologically active peptides”. Endeavour Journal, 12(2), 60-65, 1988.
 
[17]  Wang, C., Chen, T., Zhang, N., Yang, M., Li, B., Lü, X., Cao, X.,and Ling, C. “Melittin, a major component of bee venom, sensitizes human hepatocellular carcinoma cells to tumor necrosis factor related apoptosis-inducing ligand (TRAIL) induced apoptosis by activating CaMKII-TAK1-JNK/p38 and inhibiting IκBα kinase-NFκB”. Journal of Biological Chemistry, 284(6), 3804-3813, 2009.
 
[18]  Abbott, W. “A method of computing the effectiveness of an insecticide”. Journal of Economic Entomology, 18(2), 265-267, 1925.
 
[19]  Bonfanti-Almeida, J.,Gobbi, N.,andPalma, M. “Bioinsecticide action of the venom of Africanized bees Apis mellifera L. 1758 (Hymenoptera: Apidae): the most susceptible age of Diatraea saccharalis Fabricius 1794 (Lepeletier: Pyralidae) eggs to the venom action”. Journal of Venomous Animals and Toxins, 2(1), 46-51, 1996.
 
[20]  Quistad, G. B.,Dennis, P. A.,and Skinner, W. S. “Insecticidal activity of spider (Araneae), Centipede (Chilopoda), Scorpion (Scorpionida), and snake (Serpentes) Venoms”. Journal of Economic Entomology, 85(1), 33-39, 1992.
 
[21]  Quistad, G. B., Skinner, W. S., and Schooley, D. A. “Venoms of social hymenoptera—toxicity to the lepidopteran, Manduca sexta”. Journal of Insect Biochemistry, 18(6), 511-514, 1988.