World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: http://www.sciepub.com/journal/wjar Editor-in-chief: Rener Luciano de Souza Ferraz
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World Journal of Agricultural Research. 2016, 4(6), 162-165
DOI: 10.12691/wjar-4-6-2
Open AccessArticle

Distribution of Phytochemicals and Bioactivity in Different Parts and Leaf Positions of Stevia Rebaudiana (Bertoni) Bertoni- a Non-caloric, Natural Sweetener

K.L.N.W. Kumari1, D.C. Abeysinghe1 and R.M. Dharmadasa2,

1Department of Plantation Management, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP), Sri Lanka

2Industrial Technology Institute, Bauddhaloka Mawatha, Colombo 07, 00700, Sri Lanka

Pub. Date: September 06, 2016

Cite this paper:
K.L.N.W. Kumari, D.C. Abeysinghe and R.M. Dharmadasa. Distribution of Phytochemicals and Bioactivity in Different Parts and Leaf Positions of Stevia Rebaudiana (Bertoni) Bertoni- a Non-caloric, Natural Sweetener. World Journal of Agricultural Research. 2016; 4(6):162-165. doi: 10.12691/wjar-4-6-2

Abstract

Stevia rebaudiana (Bertoni) Bertoni (Asteraceae) is a small perennial herb which is widely cultivated for its sweet leaves and possesses 250-300 times the sweetness than sucrose due to the presence of steviol glycosides (mainly stevioside and rebaudioside). It is commonly known as candy leaf, sweet leaf and sugar leaf. Even though, this plant has been studied extensively for its sweetness, information on therapeutically important active components presence in stevia is scattered or lacking. Therefore, the present study was undertaken to determine the distribution of Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and Total Antioxidant Capacity (TAC) of different parts and different leaf positions of S. rebaudiana. Leaf fresh weight, dry weight and leaf area at different leaf positions were recorded. The TAC, TPC and TFC were determined using Ferric Reducing Antioxidant Power Assay (FRAP), modified Folin-Ciocalteu colorimetric method and calorimetric method respectively. Leaf fresh weight and leaf dry weight were increased with the maturity. All tested plant parts demonstrated the presence of TPC, TFC and TAC. The significantly higher TPC, TFC and TAC were reported in leaves than other parts of plant. The order of increase of active components was leaf>flower>stem>branch>root. TPC, TFC and TAC of different leaf positions revealed that TAC, was decreased gradually from immature to mature leaf (1st leaf>2nd leaf>3rd leaf>4th leaf>5th leaf). Moreover, all tested phytochemicals (phenolics and flavonoids) and antioxidant capacity were significantly higher in extracts prepared from the first leaf. Interestingly, a strong significant correlations were observed between TAC and tested secondary metabolites (TFC, R2=0.85). The results of the present study are vital important in cultivation, harvesting and quality control aspects of S. rebaudiana.

Keywords:
stevia rebaudiana antioxidant capacity flavonoids phenolics leaf positions

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References:

[1]  Manoharan, R., Sivasangari, M., Kok, J., The, W.S. and Sarveisan, K. Studies on biochemical and medicinal properties of stevia rebaudiana grown in vitro. International Journal of Ayurveda pharmaceutical, 2 (5), 169-174. 2014.
 
[2]  Brandle, J. E., Starratt, A. N. and Gijzen, M. Stevia rebaudiana: Its agricultural, biological, and chemical properties. Plant Science, 78, 527-536. 1998.
 
[3]  Debnath, M. Clonal propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. Journal of Medicinal Plants, 2, 45-51. 2008.
 
[4]  Katarzyna, G.B., Tomasz, B., Zofia, N.L., Beata, A., Anna, J., Monika, K. and Kamila, R. Inhibitory effect of stevioside on calcium influx to produce anti-hypertension. Planta Medicine, 67, 796-799. 2015.
 
[5]  Rajesh, P., Rajesh, V.K. and Thambi, M.D. Effect of Stevia rebaudiana Bertoni ethanolic extract on anticancer activity of erlisch’s ascites carcinoma induced mice. Journal of Current Biotica, 3, 549-554. 2010.
 
[6]  Sheeja, R.R. and Beena, L. Phytochemical Screening of the Leaves of Stevia rebaudiana, Bertoni. International Journal of Applied Sciences, 4, 344-347. 2015.
 
[7]  Mizutani, K. and Tanaka, O. Use of Stevia rebaudiana sweeteners in Japan. Medicinal and Aromatic Plants, 19, 178-195. 2002.
 
[8]  Satishkumar, J., Sarvanan,M.M., Seethalakshmi, I. In-vitro antimicrobial and antitumor activities of Stevia rebaudiana (Asteraceae) leaf extracts. Journal of Pharmaceutical Research, 7, 1143-1149. 2008.
 
[9]  Kedik, S.A., Yarstsev, E.I. and Stanishevskaya, I.E. Antiviral activity of dried extracted of Stevia. Journal of pharmaceutical chemistry, 43, 198-199. 2009.
 
[10]  Silva, P.A., Oliveira, D.F., Prado, N.R., Carvalho, D.A. and Carvalho, G.A. Evaluation of the antifungal activity by plant extracts against Colletotrichum gloeosporioides PENZ. Ciencia Agrotecnologia, 32, 420-428. 2008.
 
[11]  Chan, P., Xu, D.Y., Liu, J.C., Chen, Y.J., Tomlinson, B., Huang, W.P. and Cheng, J.T. The effect of stevioside on blood pressure and plasma catecholamines in spontaneously hypertensive rats. Life Science, 63, 1679-1684. 1998.
 
[12]  Lee, C.N., Wong, K., Liu, J., Chen, Y., Chen, J. and Chan, P. Inhibitory effect of stevioside on calcium influx to produce anti-hypertension. Planta Medicine, 67, 796-799. 2001.
 
[13]  Hsieh, M.H., Chan, P., Sue, Y.M., Liu, J.C., Liang, T.H., Huang, T.Y., Tomlinson, B., Chow, M.S., Kao, P.F. and Chen, Y.J. Efficacy and tolerability of oral stevioside in patients with mild essential hypertension. Clinical Theraputics, 25, 2797-2808. 2003.
 
[14]  Jeppesen, P.B., Gregersen, S., Alstrupp, K.K. and Hermansen, K. Stevio-side induces antihyperglycaemic, insulinotropic and glucagonostatic effects in vivo: studies in the diabetic Goto-Kakizaki (GK) rats. Phytomedicine, 9, 9-14. 2002.
 
[15]  Benford, D.J., Dinovi, M. and Schlatter, J. Safetyevaluation of certain food additives: Steviol Glycosides. WHO Food Additives Series (World Health Organization Joint FAO/WHO Expert Committee on Food Additives (JECFA), 54,140. 2006.
 
[16]  Das, S., Das, A.K., Murphy, R.A., Punwani, I.C., Nasution, M.P. and Kinghorn, A.D. Evaluation of the cariogenic potential of the intense natural sweeteners stevioside and rebaudioside A. Caries Research, 26, 363-366. 1992.
 
[17]  Takahashi, K., Matsuda, M., Ohashi, K., Taniguchi, K., Nakagomi, O., Abe, Y., Mori, S., Sato, N., Okutani, K. and Shigeta, S. Analysis of anti-rotavirus activity of extract from Stevia rebaudiana. Antiviral Research, 49, 15-24. 2001.
 
[18]  Takahashi, K., Iwata, Y., Mori, S. and Shigeta, S. In-vitro anti- HIV activity of extract from Stevia rebaudiana. Antiviral Research. 37-59. 1998.
 
[19]  Mohan, K., Robert, J. Hepatoprotective effects of Stevia rebaudiana Bertoni leaf extract in CCl4-induced liver injury in albino rats. Plant Science, 3, 59-61. 2009.
 
[20]  Jaroslav, P., Barbora, H. and Tuulia, H. Characterization of Stevia rebaudiana by comprehensive two dimensional liquid chromatography time of flight mass spectrometry. American Journal of Chromatography, 1150, 85-92. 2006.
 
[21]  Chatsudthipong, V. and Muanprasat, C. Stevioside and related compounds: Therapeutic benefits beyond sweetness. Pharmacology Theraputics, 121, 41-54. 2009.
 
[22]  Liu, M., Li, X.Q., Weber, C., Lee, C.Y., Brown, J., and Liu, R.H. Antioxidant and Anti-proliferative Activities of Raspberries. Journal of Agriculture and Food Chemistry, 50, 2926-2930. 2002.
 
[23]  Benzie, I.F. and Strain, J.J. The Ferric Reducing Ability of Plasma (FRAP) as a measure of “Antioxidant Power” the FRAP assay. Analytical Biochemistry, 239, 70-76. 1996.
 
[24]  Sharanabasappa, G.K., Mallikharjuna, P.B., Rajanna, L.N. and Seetharam, Y.N. Antibacterial and phytochemical evaluation of three medicinal plants. Journal of natural products. 327-334. 2007.
 
[25]  Suk Kim, Mira Yang,Ok-Hwan Lee, Suk-Nam Kang .The antioxidant activity and the bioactive compound content of Stevia rebaudiana water extracts. LWT - Food Science and Technology. 44(5), 1328-1332. 2011.
 
[26]  Abeysiri, G.R.P.I., Dharmadasa, R.M., Abeysinghe, D.C. andSamarasinghe, K. Screening of phytochemical, physio-chemical and bioactivity of different parts of Acmella oleraceae Murr. (Asteraceae), a natural remedy for toothache. Industrial crops and products, 50, 852-856. 2013.
 
[27]  Fernando, I.D.N.S., Abeysinghe, D.C. and Dharmadasa, R.M. Determination of phenolic contents and antioxidant capacity of different parts of Withania omnifera (L) Dunal from three different growth stages. Industrial crops and products, 50, 537-539. 2013.
 
[28]  Siriwardane, A.S., Dharmadasa, R.M. and Kosala, S. Distribution of withaferin A, an anticancer potential agent, in different parts of two varieties Withania somnifera (L.). Dunal. Grown Sri Lanka. Pakistan journal of Biological Sciences, 16 (3), 141-144. 2013.