World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: http://www.sciepub.com/journal/wjar Editor-in-chief: Rener Luciano de Souza Ferraz
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World Journal of Agricultural Research. 2016, 4(5), 143-146
DOI: 10.12691/wjar-4-5-3
Open AccessArticle

Phytochemical Distribution and Bioactivity of Different Parts and Leaf Positions of Pimenta Dioica (L.) Merr (Myrtaceae)

P.B.C. Panawala1, D.C. Abeysinghe1 and R.M. Dharmadasa2,

1Department of Plantation Management, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP), 60170, Sri Lanka

2Herbal Technology Section, Industrial Technology Institute, Bauddhaloka Mawatha, Colombo 07, 00700, Sri Lanka

Pub. Date: August 02, 2016

Cite this paper:
P.B.C. Panawala, D.C. Abeysinghe and R.M. Dharmadasa. Phytochemical Distribution and Bioactivity of Different Parts and Leaf Positions of Pimenta Dioica (L.) Merr (Myrtaceae). World Journal of Agricultural Research. 2016; 4(5):143-146. doi: 10.12691/wjar-4-5-3

Abstract

Pimenta dioica (L.) Merr. (Myrtaceae) is an evergreen aromatic spice widely used in perfumery, food and cosmetic industry in many parts of the world. Present study compared Total Antioxidant Capacity (TAC), Total Phenolic Content (TPC), Total Flavonoid Content (TFC), leaf area (LA), and Fresh to Dry weight ratio of Pimenta dioica leaves at different leaf positions (1st, 2nd, 3rd, 4th and 5th leaf positions) and different plant parts (immature leaf, mature leaf and bark). The TAC, TPC and TFC were determined using Ferric Reducing Antioxidant Power Assay (FRAP), modified Folin–Ciocalteu colorimetric method and calorimetric method respectively. Significantly higher TAC 562.38 ± 9.42 (mg TE/g DW), TPC 279.53±7.02 (mg GAE/g DW) were observed in leaf extract obtained from 1st leaf position. However the highest TFC 303.48 ±8.87 (mg RE/g DW) was observed in 5th leaf position. According to phytochemical distribution pattern, significantly higher TAC [619.84 ±11.98 (mg TE/g DW)], TPC [267.53 ± 5.03(mg GAE/g DW)], TFC [305.48 ±8.87 (mg RE/g DW)] were observed in extracts obtained from bud region. The potential of Pimenta dioica leaf material and bark as a fabulous raw material for food, perfumery and cosmetic industries. Further harvesting of immature leaves could be suggested for better therapeutic benefits.

Keywords:
Pimenta dioica Total Antioxidant Capacity Total Flavonoid Content Total Phenolic Content

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