World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: Editor-in-chief: Rener Luciano de Souza Ferraz
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World Journal of Agricultural Research. 2013, 1(5), 77-81
DOI: 10.12691/wjar-1-5-1
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Comparative Pharmacognostic Evaluation of Munronia Pinnata (Wall.) Theob. (Meliaceae) and Its Substitute Andrographis paniculata (Burm.f.) Wall. Ex Nees (Acanthaceae)

Dharmadasa R.M.1, , Samarasinghe K1, Adhihetty P1 and Hettiarachchi P.L2

1Herbal Technology Section, Industrial Technology Institute, Bauddhaloka Mawatha, Sri Lanka

2Rajarata University of Sri Lanka, Mihintale, Sri Lanka

Pub. Date: August 08, 2013

Cite this paper:
Dharmadasa R.M., Samarasinghe K, Adhihetty P and Hettiarachchi P.L. Comparative Pharmacognostic Evaluation of Munronia Pinnata (Wall.) Theob. (Meliaceae) and Its Substitute Andrographis paniculata (Burm.f.) Wall. Ex Nees (Acanthaceae). World Journal of Agricultural Research. 2013; 1(5):77-81. doi: 10.12691/wjar-1-5-1


Background: Munronia pinnata (Wall) Theob. (Meliaceae), a rare, therapeutically important medicinal plant, which is often adulterated by materials of Andrographis paniculata (Burm.f.) Wall. ex Nees (Acanthaceae). However, adulteration of M. pinnata with A. paniculata without scientifically proven data on important quality standards might adversely affect the therapeutic properties of herbal drugs. Methodology: Therefore, the present study was undertaken to establish a comparative quality standards on morphological, anatomical, powder microscopical, phytochemical, physicochemical and antioxidant activity of M. pinnata and A. paniculata by using established protocols. Principal Findings: Results demonstrated that M. pinnata could be distinguished from A. paniculata by comparing polymorphic morphological characters, anatomical and powder microscopic characters. Major phytochemical groups were present in leaves, stem and roots of both plants. Results of TLC exhibited the highest number of common spots in leaf (Rf 0.10, 0.23, 0.30, 0.56, 0.86 and 0.96) followed by stem and root extracts for both M. pinnata and A. paniculata. Both plant species possess notable total antioxidant capacity (TAC) of all three parts tested. However, higher TAC was exhibited in A. paniculata compared to M. pinnata. Order of increase of TAC was leaf > stem > root for M. pinnata and stem > leaf > root for A. paniculata. Conclusions/Significance: The presence of certain similarities in major phytochemical groups, and in antioxidant capacity of M. pinnata and A. paniculata to some extent justifies the use of A. paniculata as a substitute for M. pinnata in traditional systems of medicine in Sri Lanka which needs to be confirmed after further clinical trials.

Meliaceae acanthaceae Munronia pinnata Andrographis paniculata substitute phytochemical parameters antioxidant capacit

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[1]  Ping Li, Lian-Wen Qi., E-Hu Liu., Jian-Liang Zhou., Xiao-Dong Wen., Analysis of Chinese herbal medicines with holistic approaches and integrated evaluation models. Trends in Analytical Chemistry 27, 66-77. 2008.
[2]  Naumovski, V.R., Wannit Tongkao-on., Benjamin Kimble., Vincent L, Qiao, Lin Beilun., Kong, M. Li., Basil Roufogalis., Yang Depo, Yao Meicun., George Q, Multiple Chromatographic and Chemometric Methods for Quality Standardization of Chinese Herbal Medicines. World Science and Technology 12, 99-106 2010.
[3]  Springfield, E.P, Eagles, P.K.F, Scott, G, Quality assessment of South African Herbal Medicines by means of HPLC fingerprinting. J. of Ethnobotany 101, 75-83. 2005.
[4]  WHO, Quality Control Methods For Herbal Materials, World Health Organization, 1998.
[5]  Opara I, Elizabeth, The efficacy and safety of Chinese Herbal Medicines. Br. J. Nut. 91,171-193, 2004.
[6]  Chan, T.Y, Critchley, J.A., Usage and adverse effect of Chinese herbal medicines. Hum. Exp. Toxicol. 15, 5-12., 1996.
[7]  Li, X.M, Zhang, T.F, Huang, C.K, Srivastava, K., Teper, A. A., Zhang, L, Schofield, B. H, Sampson, H.A, Food Allergy Herbal Formula-1 (FAHF-1) blocks peanut-induced anaphylaxis in amurine model. J. Allergy. Clin. Immunol. 108, 639-646. 2001.
[8]  Dennis V.C. Awang, Standardization of Herbal Medicinal Products. Proc. XXVI IHC – Future for Medicinal and Aromatic Plants. Eds. L.E. Craker et al. Acta Hort. 629, ISHS. 2004.
[9]  Jayaweera, B.M.A, Medicinal plants (Indigenous and Exotic) used in Ceylon- part (IV). 59 Natn. Sci. Coun. Sri Lanka. 1982.
[10]  Mabberley, Meliaceae. In: Dassanayake MD, Forsberg FR. (ed.) A Revised Handbook to the Flora of Ceylon: 229-239 Amerind. Publ. Co. Ltd, New Delhi, India. 1995.
[11]  Shahid Akbar, Andrographis paniculata: A Review of Pharmacological Activities and Clinical Effects. Alternative Medicine Review 16, 66-68, 2011.
[12]  Trease and Evans, W.C, Trease and Evans’ Pharmacognosy (fifteenth editions ed.). Cambridge university press, Great Britain. 2006.
[13]  Farnsworth, N.R, Biological and phytochemical screening of plants. Journal of Pharmaceutical Science. 55, 225-276, 1966.
[14]  Stahl, E, Thin Layer Chromatography. A Laboratory hand book. 2nd Ed. 854-859 Springer international student edition, 1969.
[15]  Benzie, I.F. and Strain, J.J, The Ferric Reducing Ability of Plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal. Biochem., 239, 70-76 1996.
[16]  Metcalfe, C.R., Chalk, L, Anatomy of Dicotyledons (First edition ed., Vol. 1) London : Oxford university Press. 1957.
[17]  Sudhakaran, 2012. Botanical pharmacognosy of Andrographis paniculata (Burm.F. Wall.Ex. Nees. Pharmacognosy Journal 4 , 1-10,2012.
[18]  Branislava Lakušić, Violeta Popov, and Dušanka Runjajić-Antić, Morpho-anatomical characteristics of the raw material of the herbal Drug olivae folium and its counterfeits. Arch. Biol. Sci., Belgrade, 59 (3), 187-192 2007.
[19]  Pravin H. Nikam, Joseph Kareparamban., Aruna Jadhav and Vilasrao Kadam., Future Trends in Standardization of Herbal Drugs. Journal of Applied Pharmaceutical Science, 2, 38-44, 2012.
[20]  Manoj Kumar Pandey, Singh, G.N., Rajeev, K.R., Sharma and Sneh Lata, Physicochemical standardization of Andrographis paniculata (nees): an ayurvedic drug Inventi Impact: Pharm Analysis & Quality Assurance 2011. [ cited 2013 Jun 22]. Available From http://www.
[21]  Suganya Tachakittirungrod, Siriporn Okonogi, Sombat Chowwanapoonpohn. Study on antioxidant activity of certain plants in Thailand:Mechanism of antioxidant action of guava leaf extract. Food Chemistry 103, 381-388, 2007
[22]  Eugine Leo Prakash, S. and R. Manavalan, Development, characterisation and/toxicity evaluation of nanoparticles of Andrographolide. Int J Pharm Sci, 4, 497-501, 2012.
[23]  Mondal, A.K, Advanced plant taxonomy; New Delhi: New central Book agency. 2009.