World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: Editor-in-chief: Rener Luciano de Souza Ferraz
Open Access
Journal Browser
World Journal of Agricultural Research. 2014, 2(4), 163-167
DOI: 10.12691/wjar-2-4-5
Open AccessArticle

Secondary Metabolites Contents and Antioxidant Capacities of Acmella Oleraceae Grown under Different Growing Systems

D.C. Abeysinghe1, S.M.N.K. Wijerathne1 and R.M. Dharmadasa2,

1Department of Plantation Management, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP)

2Industrial Technology Institute, Bawddhaloka Mawatha, Colombo, Sri Lanka

Pub. Date: July 16, 2014

Cite this paper:
D.C. Abeysinghe, S.M.N.K. Wijerathne and R.M. Dharmadasa. Secondary Metabolites Contents and Antioxidant Capacities of Acmella Oleraceae Grown under Different Growing Systems. World Journal of Agricultural Research. 2014; 2(4):163-167. doi: 10.12691/wjar-2-4-5


Acmella oleraceae (L.) R.K. Jansen (Asteraceae) is a therapeutically important medicinal plant used in traditional systems of medicine. Present study was undertaken to compare the total phenolic contents (TPC), total flavonoid contents (TFC), total antioxidant capacity (TAC) and Thin Layer Chromatography (TLC) profiles of areal parts and callus extracts of A. oleraceae grown under different growing systems (field grown, hydroponically grown and callus culture. Callus established in Murasige and Skoog (MS) medium, areal parts of field grown and hydroponically grown plants were extracted in 80% methanol. TPC, TFC and TAC were carried out by using colorimetric Folin-Ciocalteu method, aluminum nitrate method and Ferric Reducing Antioxidant Power (FRAP) assay respectively. TLC profiles were developed using established protocol. The best callus growth and the highest mean callus weight were observed in leaf explants established in MS medium supplemented with 2 mg/L BA and 1 mg/L IBA. Comparatively higher TPC (11.45 ± 0.17), TFC (12.33 ± 0.92) and TAC (10.27 ± 0.28) were observed in hydroponically grown plants. Order of TPC, TFC and TAC were increased as callus<field grown plants< hydroponically grown plants. The presence of higher TPC, TFC and TAC in hydroponically grown plants opens a new window for use of hydroponic system for growing of A. oleraceae for better secondary metabolites content.

Acmella oleracea antioxidant capacity Asteraceae flavonoids phenolics

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Pandey, V. and Agrawal, V. (2009).Efficient micro propagation protocol of Spilanthesacmella L. possessing strong antimalaria activity, In vitro cellular and development biology- plant, 45, 491-499.
[2]  Jayaweera, D.M.A., (1981). Medicinal Plants National Science Council of Sri Lanka, Colombo, Sri Lanka, 111, 71.
[3]  Dias A.M.A., Santosa, P., Seabraa, I.J., Junior, R.N.C., Braga, M.E.M. and De Sousa, H.C (2011). Spilanthol from Spilanthes acmella flowers, leaves and stems obtained by selective super critical carbon dioxide extraction. The Journal of Supercritical fluids (in press).
[4]  Chandur, U., Shashidhar, S., Chandrasekar, S.B., Bhanumathy, M. and Midhun, T. (2011). Phytochemical evaluation and anti-arthritic activity of root of Saussure alappa. Pharmacologia, 2, 265-267.
[5]  Gangopadhyay,M.,Dewanjee, S., Chakraborty D. and Bhattacharya, S. (2011). Role of exogenous phytohormones on growth and plumbagin accumulation in Plumbago indica hairy roots and conservation of elite root clones via synthetic seeds. Ind. Crops Prod, 33, 445-450.
[6]  Morard, P. (1995). Les cultures végétales hors sol. Publications agricoles, Agen, France, ISBN: 2-9509297, 303.
[7]  Lenora, R.D.K., Dharmadasa, R.M., Abeysinghe, D.C. and Arawwawala, L.D.A.M. (2012).Investigation of Plumbagin Content in Plumbagoindica Linn.Grown under Different Growing Systems.Pharmacologia, 3: 57-60.
[8]  Abeysinghe, D.C., Li, X., Sun, C., Zhang, W., Zhou, C. and Chen, k., (2007). Bioactive compounds and antioxidant capacities in different edible tissues of citrus fruits of four species, Food Chemistry, 104, 1338-1344.
[9]  Abeysiri, G.R.P.I., Dharmadasa, R.M. and Abeysinghe, D.C, and K. Samarasinghe (2013).Screening of phytochemical, physico-chemical and bioactivity of different parts of Acmella oleraceae Murr. (Asteraceae), a natural remedy for toothache. Industrial Crops and Products, 50, 852-856.
[10]  Benzie IFF and Strain JJ (1996) The ferric reducing ability of plasma as a measure of antioxidant power: The FRAP assay. Journal of analytical Biochemistry, 293: 70-76.
[11]  Singh, M. and Chaturvedi, R. (2012). Screening and quantification of an antiseptic alkylamide, spilanthol from in vitro cell and tissue cultures of Spilanthes acmella Murr. Industrial Crops and Products, 321-328.
[12]  Menkovic, N., Savikin-Fodulovic, K. and Savin, K. (2000). Chemical composition and seasonal variations in the amount of secondary compounds in Gentianalutealeaves and flowers. Planta Med, 66, 178-180.
[13]  Giri, L., Dhyani, P., Rawat, S., Indra, D., Shyamal, B., Nandi, K., Rawal, R.S. and Pande, V. (2012) In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenariaedgeworthii: A rare Himalayan medicinal orchid. Industrial Crops and Products 39, 1-6.
[14]  Gontier, E., Clement, A., Tran, T.L.M., Gravot, A., Lievre, K., Guckert, A. and Bourgaud, F. (2002). Hydroponic combined with natural or forced root permeabilization: A promising technique for plant secondary metabolite production. Plant Science, 163, 723-732.
[15]  Aman D. Kaur, V. Ravichandran, Prateek K.Jain, Ram K. Agrawal (2008). High-performance thin layer chromatography method for estimation of conessine in herbal extract and pharmaceutical dosage formulations. 46(2), 391-394.
[16]  Dharmadasa, R.M., Samarasinghe K, Adhihetty,P. and Hettiarachchi P.L. (2013) Comparative Pharmacognostic Evaluation of Munronia pinnata (Wall.) Theob. (Meliaceae) and Its substitute Andrographis paniculata (Burm.f.) Wall. Ex Nees (Acanthaceae).
[17]  Dharmadasa, R.M., Asitha Siriwardana, Kosala Samarasinghe, and Adhihetty, P. (2013). Standardization of Gyrinops Walla Gaertn. (Thymalaeaceae): Newly Discovered, Fragrant Industrial Potential, Endemic Plant from Sri Lanka. World Journal of Agricultural Research 1, No. 6: 101-103.