World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: http://www.sciepub.com/journal/wjar Editor-in-chief: Rener Luciano de Souza Ferraz
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World Journal of Agricultural Research. 2014, 2(4), 163-167
DOI: 10.12691/wjar-2-4-5
Open AccessArticle

Secondary Metabolites Contents and Antioxidant Capacities of Acmella Oleraceae Grown under Different Growing Systems

D.C. Abeysinghe1, S.M.N.K. Wijerathne1 and R.M. Dharmadasa2,

1Department of Plantation Management, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP)

2Industrial Technology Institute, Bawddhaloka Mawatha, Colombo, Sri Lanka

Pub. Date: July 16, 2014

Cite this paper:
D.C. Abeysinghe, S.M.N.K. Wijerathne and R.M. Dharmadasa. Secondary Metabolites Contents and Antioxidant Capacities of Acmella Oleraceae Grown under Different Growing Systems. World Journal of Agricultural Research. 2014; 2(4):163-167. doi: 10.12691/wjar-2-4-5

Abstract

Acmella oleraceae (L.) R.K. Jansen (Asteraceae) is a therapeutically important medicinal plant used in traditional systems of medicine. Present study was undertaken to compare the total phenolic contents (TPC), total flavonoid contents (TFC), total antioxidant capacity (TAC) and Thin Layer Chromatography (TLC) profiles of areal parts and callus extracts of A. oleraceae grown under different growing systems (field grown, hydroponically grown and callus culture. Callus established in Murasige and Skoog (MS) medium, areal parts of field grown and hydroponically grown plants were extracted in 80% methanol. TPC, TFC and TAC were carried out by using colorimetric Folin-Ciocalteu method, aluminum nitrate method and Ferric Reducing Antioxidant Power (FRAP) assay respectively. TLC profiles were developed using established protocol. The best callus growth and the highest mean callus weight were observed in leaf explants established in MS medium supplemented with 2 mg/L BA and 1 mg/L IBA. Comparatively higher TPC (11.45 ± 0.17), TFC (12.33 ± 0.92) and TAC (10.27 ± 0.28) were observed in hydroponically grown plants. Order of TPC, TFC and TAC were increased as callus<field grown plants< hydroponically grown plants. The presence of higher TPC, TFC and TAC in hydroponically grown plants opens a new window for use of hydroponic system for growing of A. oleraceae for better secondary metabolites content.

Keywords:
Acmella oleracea antioxidant capacity Asteraceae flavonoids phenolics

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