World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: Editor-in-chief: Rener Luciano de Souza Ferraz
Open Access
Journal Browser
World Journal of Agricultural Research. 2014, 2(3), 109-114
DOI: 10.12691/wjar-2-3-4
Open AccessArticle

Some Physiological Parameters as Screening Tools for Drought Tolerance in Bread Wheat Lines (Triticum aestivam L.)

A.A.S. Ahmed1, R. Uptmoor2, M.A. El-Morshidy3, K.A. Kheiralla3, M.A. Ali4 and Naheif E.M. Mohamed1,

1Dep. of Agronomy, Faculty of Agriculture, Sohag University, Egypt

2Institute of Biological Production Systems, Hannover University, Germany

3Dep. of Agronomy, Faculty of Agriculture, Assiut University, Egypt

4Dep. of Agronomy, Faculty of Agriculture, South Valley University, Egypt

Pub. Date: May 06, 2014

Cite this paper:
A.A.S. Ahmed, R. Uptmoor, M.A. El-Morshidy, K.A. Kheiralla, M.A. Ali and Naheif E.M. Mohamed. Some Physiological Parameters as Screening Tools for Drought Tolerance in Bread Wheat Lines (Triticum aestivam L.). World Journal of Agricultural Research. 2014; 2(3):109-114. doi: 10.12691/wjar-2-3-4


Two greenhouse experiments were carried out at the Institute of Biological Production Systems, Leibniz Universität Hannover, Germany during 2008/2009 and 2009/2010 growing seasons to study the influence of the osmotic adjustment (OA) capacity, relative water content (RWC) and specific leaf area (SLA) on tolerance to drought in 22 breeding lines, two parents and tolerant cultivar (Sahel 1) of bread wheat (Triticum aestivam L.) under drought conditions. Differences were seen in of the OA, RWC and of the different genotypes. Mean over all of OA, RWC and for breeding lines were -0.51 Mpa, 83.28% and 116.56 cm²g-1, respectively. Four of the breeding lines showed the greatest osmotic adjustment capacities, high RWC and good values under drought stress conditions better than the tolerant cultivar. The heritability of OA, RWC and was 0.56, 0.49 and 0.88, respectively. The results indicated that osmotic adjustment, as well as RWC and could be used as screening tools for drought resistant bread wheat genotypes in the greenhouse. This study also demonstrated the appropriate greenhouse screening methodology in this regard.

wheat drought osmotic adjustment heritability

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Arjenaki, F. G., R. Jabbari1 and A. Morshedi, (2012). Evaluation of Drought Stress on Relative Water Content, Chlorophyll Content and Mineral Elements of Wheat (Triticum aestivum L.) Varieties. Intl J Agri Crop Sci. 11: 726-729.
[2]  Blum, A. (1996). Crop response to drought and the intepretation of adaptation. Plant Growth Regulation, 20: 135-148.
[3]  Blum, A., J. Zhang and H.T. Nguyen. (1999). Consistent differences among wheat cultivars in osmotic adjustment and their relationship to plant production. Field Crops Res., 64: 287-291.
[4]  Chimenti, C.A., Marcantonio, M., Hall, A.J., (2006). Divergent selection for osmotic adjustment results in improved drought tolerance in maize (Zea mays L.) in both early growth and flowering phases. Field Crops Res. 95, 305-315.
[5]  Dhanda, S.S. and D.S. Sethi. (1996). Genetics and interrelationships of grain yield and its related traits in bread wheat under irrigated and rainfed conditions. Wheat Inform. Service, 83: 19-27.
[6]  Dichio, B., Xiloyannis, C., Sofo, A., Montanaro, G. (2005). Osmotic regulation in leaves and roots of olives trees during a water deficit and rewatering. Tree Physiol. 26, 179-185.
[7]  El-Rawi, K. and A.M. Khalafalla. (1980). Design and analysis of agricultural experiments, El Mousel Univ., Iraq, 19.
[8]  Farouk, S. (2011). Osmotic adjustment in wheat flag leaf in relation to flag leaf area and grain yield per plant. J. of Stress Physiology & Biochemistry, Vol. 7 No. 2, pp. 117-138.
[9]  Gaballah, M.S., B. Abou, H. Leila, A. El-Zeiny and S. Khalil, (2007). Estimating the performance of salt stressed sesame plant treated with antitranspirants. J. Applied Sci. Res., 3: 811-817.
[10]  Golparvar, A.R. (2003). Genetic analysis of drought resistance in bread wheat cultivars. Ph. D Thesis, Islamic Azad Univ. Branch of Science and Res. of Tehran, pp: 287(In Persian).
[11]  Golparvar, A.R. and A. Ghasemi-Pirbalouti and H. Madani. (2006). Genetic control of some physiological attributes in wheat under drought stress conditions. Pakistan J. of Biological Sciences 9 (8): 1442-1446.
[12]  González A., I. Martín and L. Ayerbe (2008). Yield and Osmotic Adjustment Capacity of Barley Under Terminal Water-Stress Conditions. J. Agronomy & Crop Science, Volume 194, Issue 2: 81-91.
[13]  González, A. and L. Ayerbe (2011). Response of coleoptiles to water deficit: growth, turgor maintenance and osmotic adjustment in barley plants (Hordeum vulgare L.). Agricultural Sciences, 2: 159-166.
[14]  Lazacano-Ferrat, I. and C. J. Lovat, (1999). Relationship between relative water content, nitrogen pools, and growth of Phaseolus vulgaris L. and P. acutifoolius A. Gray during water deficit. Crop. SCI., 39: 467-475.
[15]  Ludlow MM, Muchow RC (1990) A critical evaluation of traits for improving crop yields in water limited environments. Advances in Agronomy 43: 107-153.
[16]  Morgan, J.M. (1977 b). Differences in osmoregulation between wheat genotypes. Nature (London) 270: 234-235.
[17]  Mayer, J. and G. Gozlan. (1982). Infraed thermal sensing of plant canopies a screening technique for dehydration avoidance in wheat. Field Crops Res., 5: 137-146.
[18]  M Sinclair, T.R. and M.M. Ludlow, (1985). who taught plants thermodynamics? The unfulfilled potential of plant water potential. Aust. J. Plant Physiol. 33: 213-217. M., Hare, R.A. and Fletcher, R.J., 1986. Genetic variation. J. Morgan, J.M., 1992 Agric. jjkkjkj.
[19]  Morgan, J.M., Hare, R.A. and R.J Fletcher, (1986). Genetic variation in osmoregulation in bread and durum wheat and its relationship to grain yield in arrange of field environments. Aus. J. Agric. Res., 37: 449-457.
[20]  Morgan, J.M., (1995). Growth and yield of wheat lines with differing osmoregulative capacity at high soil water deficit in seasons of varying evaporative demand. Field Crops Res. 40, 143-152.
[21]  Marcelis, L.F.M., Heuvelink, E., Goudriaan, J., (1998). Modelling biomass production and yield of horticultural crops: a review. Sci. Hort. 74, 83-111.
[22]  Morgan, J.M. (1999). Pollen grain expression of a gene controlling differences in osmoregulation in wheat leaves: A simple breeding method. Aust. J. Agric. Res. 50: 953-962.
[23]  Moinuddin, R. A. Fischer, K. D. Sayre, and M. P. Reynolds (2005). Osmotic Adjustment in Wheat in Relation to Grain Yield under Water Deficit Environments. Agron. J. 97: 1062-1071.
[24]  Mart"ınez JP, Ledent JF, Bajji M, Kinet JM, Lutts S. (2003). Effect of water stress on growth, Naþ and Kþ accumulation and water use efficiency in relation to osmotic adjustment in two populations of Atriplex halimus. Plant Growth Regul, 41: 63-73.
[25]  Mart"ınez JP, Ledent JF, Bajji M, Kinet JM, Lutts S. (2004). Is osmotic adjustment required for water stress resistance in the Mediterranean shrub Atriplex halimus L. J. of Plant Physiology 161: 1041-1051
[26]  Martin M., F. Miceli, J. A. Morgan, M. Scalet and G. Zerbi (2009). Synthesis of Osmotically Active Substances in Winter Wheat Leaves as Related to Drought Resistance of Different Genotypes. J. of Agro. and Crop Science V. 171 Is. 3: 176-184.
[27]  Passioura JB. (1996). Drought and drought tolerance. Plant Growth Regulation 20, 79-83.
[28]  Passioura JB. (2007). The drought environment: physical, biological and agricultural perspectives. Journal of Experimental Botany 58, 113-117.
[29]  Ramos, M.L.G., R. Parsons, J. I. Sprent and E. K. Games, (2003). Effect of water stress on nitrogen fixation and nodule structure of common bean. Pesq. Agropec. Brasilia., 38: 339-347.
[30]  Schonfeld, M.A., R.C. Johnson, B.F. and Carwer, D.W. Mornhinweg, (1988). Water relations in winter wheat as drought resistance indicators. Crop. Sci., 28: 526-531.
[31]  Snedecor, G.W. and W.G. Cochran. (1980). Statistical methods. 7th ed. Lowa State Unv. Press., Ames., Lowa, U.S.A.
[32]  Siddique, M.R.B., A. Hamid and M.S. Islam. (2000). Drought stress effects on water relations of wheat. Bot. Bull. Acad. Sin., 41: 35-39.
[33]  Suprunova T, Krugman T, Fahima T, Chrn G, Shams I, Korol A, Nevo E (2004) Differential expression of dehydrin genes in wild barley (Hordeum spontaneum), associated with resistance to water deficit. Plant Cell Environ. 27: 1297-1308.
[34]  Slafer, G.A. and J.L. Araus. (1998). Improving wheat responses to abiotic stresses. In: Proceedings 9th International Wheat Genetic Symposium. Saskatoon, Canada, 1: 201-213.
[35]  Tahara, M., B. F. Carver, R. C. Johnson and E.L. Smith. (1990). Relationship between relative water content during reproductive development and winter wheat grain yield. Euphytica, 49: 255-262.
[36]  Walker, T.T. (1960). The use of a selection index technique in the analysis of progeny row data. Emp. Cott. Gr. Rev. 37: 81-107.
[37]  Yamasaki, S. and L. R. Dillenburg, (1999). Measurements of leaf relative water content in araucaria angustifolia. R. Bras. Fisiol. Veg., 11 (2): 69-75.