In Vitro Screening of Bacillus Isolates for Biological Control of Early Blight Disease of Tomato in Shambat Soil

Saifeldeen A. Abdalla¹, Soad A. A. Algam², Elshiekh A. Ibrahim³ and Ahmed M. El Naim^3,

¹Department of Plant Protection, Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied, Sudan

²Department of Plant Protection, Faculty of Agriculture, University of Khartoum, Khartoum, Shambat, Sudan

³Department of Crop Sciences, Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied, Sudan

Cite this paper:
Saifeldeen A. Abdalla, Soad A. A. Algam, Elshiekh A. Ibrahim and Ahmed M. El Naim. In Vitro Screening of Bacillus Isolates for Biological Control of Early Blight Disease of Tomato in Shambat Soil. World Journal of Agricultural Research. 2014; 2(2):47-50. doi: 10.12691/wjar-2-2-3

Abstract

Rhizosphere bacteria are one of the most potential biological control agents in the plant disease protection. Bacillus species as a group offer several advantages over other bacteria for protection against pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. Five soil samples from tomato rhizosphere were collected from shambat area, Khartoum State, Sudan. Bacillus isolates were isolated from the rhizosphere of tomato to use as natural bio-control agents. They were screened for antagonism in vitro against Alternaria alternata causal agents of early blight disease of tomato. Serial dilution technique was adopted for the isolation of Bacillus species. Only 27 out of 45 Bacillus isolates showed antagonistic properties. Four out of the 27 isolates showed antagonism (Bacillus B25, B35, B41, B45) were identified to the species level by bacteriological assay (morphological and biochemical tests).

Keywords:
biological control; Bacillus spp. Alternaria alternate tomato early blight

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