World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: http://www.sciepub.com/journal/wjar Editor-in-chief: Rener Luciano de Souza Ferraz
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World Journal of Agricultural Research. 2022, 10(1), 20-29
DOI: 10.12691/wjar-10-1-4
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Bacterial Communitie’s Diversity of Rhizosphere’s Soils of Two Legumes, Cajanus cajan and Milletia laurentii, Revealed by Illumina Miseq Sequencing of 16S rRNA Gene

Alvychelle Benith Banga1, 2, , Augustin Aimé Lebonguy1, Angélique Espérance Lembella Boumba1, 3 and Joseph Goma-Tchimbakala1, 3

1Institut National de Recherche en Sciences Exactes et Naturelles, Brazzaville République du Congo

2Faculté des Sciences et Techniques, Brazzaville, République du Congo

3Ecole Nationale Supérieure d’Agronomie et de Foresterie, Brazzaville République du Congo

Pub. Date: June 21, 2022

Cite this paper:
Alvychelle Benith Banga, Augustin Aimé Lebonguy, Angélique Espérance Lembella Boumba and Joseph Goma-Tchimbakala. Bacterial Communitie’s Diversity of Rhizosphere’s Soils of Two Legumes, Cajanus cajan and Milletia laurentii, Revealed by Illumina Miseq Sequencing of 16S rRNA Gene. World Journal of Agricultural Research. 2022; 10(1):20-29. doi: 10.12691/wjar-10-1-4

Abstract

Microbial organic fertilizers have been shown to boost plant productivity. These microorganisms of interest are more numerous in the soil around the roots or rhizosphere. Objective of this study was to assess bacterial communities’ diversity of in the rhizosphere of two legumes, Milletia laurentii and Cajanus cajan, growing on the same soil. First of all, the levels Mg, N, Fe, C total, P, NH4+ and particle size were determined by spectrophotometry, Kjeldahl method, Olsen method, Walkey-Black method, Nessler reagent, DEB method and Robinson pipette method, respectively. Next, bacterial diversity was determined by Sequencing Illumina Miseq of 16S rRNA gene. Results showed that contents of carbon, total nitrogen, ammoniacal nitrogen, phosphorus, iron and magnesium were slightly elevated in Milletia rhizosphere compared to Cajanus. According to the USDA's textural triangle, both soils have a sandy loam soil texture. In terms of diversity, all OTUs (1434) were divided into 30 phyla, 50 classes, 158 families and 314 genera for the 2 soils. Proteobacteria (58.62% - 48.71%), Acidobacteria (27.29% - 9.46%), Firmicutes (8.26% - 7.21%) and Bacteroidetes (13.70% - 2.53%) were most dominant phyla in both rhizospheres (Cajanus - Milletia). The most dominant classes were Alphaproteobacteria (51.44% - 38.90%), Acidobacteriia (26.57% - 8.67%), Bacilli (8.19% - 7.18%), Sphingobacteria (9.83% - 2.50%) and Gammaproteobacteria (4.27% - 3.39%). At the family level, Hyphomicrobiaceae (35.05%-24.22%), Bradyrhizobiaceae (17.32%-11.70%) and Bacillaceae (18.98%-6.49%) were most abundant. Finally, Acidobacterium (26.55%-4.58%), Rhodoplanes (21.63%-7.50%), Bradyrhizobium (17.27%-1.96%) and Bacillus (6.43%-6.29%) were the most abundant genera. Thus, bacterial diversity of the rhizosphere of these two legumes encourages their use for the isolation of bacteria with biofertilizing potential.

Keywords:
Illumina - Miseq rhizosphere bacterial diversity

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