World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: http://www.sciepub.com/journal/wjar Editor-in-chief: Rener Luciano de Souza Ferraz
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World Journal of Agricultural Research. 2019, 7(4), 124-131
DOI: 10.12691/wjar-7-4-2
Open AccessArticle

Antifungal Potential of Curcuma longa (Tumeric) and Zingiber officinale (Ginger) against Alternaria alternata Infecting Spinach in Kenya

Kirarei Ezra Kipkogei1, , Kipsumbai Pixley Kiptui1 and Kiprop Ezekiel1

1Department of Biological Sciences, University of Eldoret, P.O Box 1125-30100, Eldoret, Kenya

Pub. Date: October 28, 2019

Cite this paper:
Kirarei Ezra Kipkogei, Kipsumbai Pixley Kiptui and Kiprop Ezekiel. Antifungal Potential of Curcuma longa (Tumeric) and Zingiber officinale (Ginger) against Alternaria alternata Infecting Spinach in Kenya. World Journal of Agricultural Research. 2019; 7(4):124-131. doi: 10.12691/wjar-7-4-2

Abstract

Spinach diseases caused by Alternaria spp. are one of the most significant devastating pathogens to spinach in Kenya and worldwide. Alternaria alternata has been associated with great losses in spinach both in total biomass yield and leaf quality. The pathogen has been traditionally controlled using synthetic fungicides which are expensive and harmful to both humans and environment. This study aimed at investigating the efficacy of the extracts of two plants; Curcuma longa (Tumeric) and Zingiber officinale (Ginger) against Alternaria alternata both in in vitro and in vivo conditions. Absolute ethanol, water, ethyl acetate and methanol were the solvents used in extraction of Curcuma longa and Zingiber officinale rhizome extracts. Decoctions were screened for antimycotic potential using the poisoned food technique. Results from this study revealed that Curcuma longa and Zingiber officinale extracts had varying degree of antifungal activity against the Alternaria alternata depending on the solvent used for the extraction and the concentration. Methanolic extracts of Curcuma longa and Zingiber officinale demonstrated the highest antifungal activity which was significant (p≤0.05) against the Alternaria alternata compared to ethanolic, ethyl acetate and aqueous extracts with percentage inhibition of 64% and 57%, respectively at the concentration of 50 mg/ml. At the concentration of 25 mg/ml and 50 mg/ml the percent inhibition on the fungal growth was not significant (p≤0.05) from the 8th day for all the solvents in both turmeric and ginger. Foliar spray with the extracts was found to be effective in lowering disease severity. Curcuma longa displayed the highest percent decrease index in comparison to Zingiber officinale with percent disease decrease index of 57.70% and 53.84%, respectively. The findings indicated methanol as the most suitable solvent for descending in the use of Curcuma longa and Zingiber officinale extracts in controlling Alternaria alternata associated with leaf spot of spinach in Kenya.

Keywords:
spinach plant extracts antifungal plant disease control Alternaria alternata Curcuma longa Zingiber officinale

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References:

[1]  Sato, A., Takeda, H., Oyanagi, W., Nishihara, E. & Murakami, M. (2010). Reduction of cadmium up take in spinach, Spinacia oleracea L. by soil amendment with animal waste compost. 181, 298-304.
 
[2]  Wachira, M. J., Mshenga, M. P. & Saidi, M. (2014). Comparison of profitability of small scale green house and open-field tomato production systems in Nakuru North District, Kenya. Asian Journal of Agricultural Sciences 6, 54-61.
 
[3]  MoA. (2016). External Evaluation of Horticulture and Food Security Program. Ministry of Agriculture, Kenya.
 
[4]  FAOSTAT. (2016). Crops/ Regions/ World list of Production Quality of Spinach. The Food and Agricultural Organisation of the United Nations. Rome, Italy.
 
[5]  MoA. (2000). Local and Export Vegetables: growing Manual. Ministry of Agriculture, Rural Development, Kenya & Japan International Cooperation Agency, Printed by Agricultural Information Resource Centre, Nairobi, Kenya.
 
[6]  Singh, V., Vhrivastava, A., Jadon, S., Wahi, N., Singh, A. & Sharma, N. (2015). Alternaria Diseases of Vegetable Crops and its Management Control to Reduce the Low Production. International Journal of Agriculture Sciences, 13, 834-840.
 
[7]  Thomma. B. (2003). Alternaria spp. from general saprophyte to specific parasite. Mol. Plant Pathol, 4, 226-236.
 
[8]  Marraiki, N. N., Siddiqui, I., Rizwana, H. & Javaid, J. (2012). First report of Alternaria alternata leaf spots on spinach in Saudi Arabia. The Journal of Animal & Plant Sciences, 22 (1), 247-248.
 
[9]  Czajka, A., Czubatka, A., Sobolewski, J. & Robak, J. (2015). First Report of Alternaria Leaf Spot Caused by Alternaria alternata on Spinach in Poland, 99 (5), 729-738.
 
[10]  Aslam, S, H., Aslam, M U., Abbas, A., Ali, M, A., Alam, M, W., … Amrao, L. (2019). First Report of Leaf Spot of Spinach Caused by Alternaria alternata in Pakistan. Plant Disease, 103 (6), 1430-1442.
 
[11]  Kuna, P., Kaczmarek, J. & Kupczyk, M. (2011). Efficacy and safety of immunotherapy for allergies to Alternaria alternata in children. J. Allerg. Clin. Immunol, 127, 502-508.
 
[12]  Mizubuti, G. S. E., Junior, V. L. & Forbes, G. A. (2007). Management of late blight with alternative products. Pest Technol. 1(2), 106-116.
 
[13]  Humaria, R. (2015). Exploiting antifungal potential of ginger for the management of Alternaria alternata, the cause of leaf spot disease of spinach. Mycopathology, 13 (2), 97-104.
 
[14]  Shafique, S., Shafique, S., Bajwa, R., Akhtar, N. & Hanif, S. (2011). Fungitoxic activity of aqueous and organic solvent extracts of Tagetes erectus on phytopathogenic fungus-Ascochytarabiei. Pak. J. Bot, 43, 59-64.
 
[15]  Tatsadjieu, N. L., Dongmo, P. M. J., Ngssoum, M. B., Etoa, F. X. & Mbofung, C. M. F. (2009). Investigation on the essential oil of Lippi arugosa from Cameroon for its potential use as antifungal agent against Aspergillus flavus. Food Cont., 20, 161-166.
 
[16]  Muthomi, J. W., Geraldin, M. W. L., Maina, J. W. & Rama, D. N. (2017). In vitro activity of plant extracts against some important plant pathogenic fungi of tomato. Australian journal of crop science, 11(06), 683-689.
 
[17]  Pankaj, S. & Richa, S. (2017). Antifungal screening of Curcuma longa and Zingiber officinale against dermatophytes causing superficial Mycosis. Int. J. Pharm Res Health Sci. 5, 1980-1983.
 
[18]  Malkhan, S. G. Shahid, A., Masood, A. & Kangabam, S. S. (2012). Efficacy of plant extracts in plant disease management. Journal of Agricultural Sciences, 3 (3), 425-433.
 
[19]  Ncube, N., Afolayan, S.A.J. & Okoh, A. I. (2008). Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. African Journal of Biotechnology, 7, 1797-1806.
 
[20]  Noman, E., Al-Gheethi, A. A., Rahman, N. K., Talip, B., Mohamed, R. and Kadir, O. A. (2018). Single Spore Isolation as a Simple and Efficient Technique to obtain fungal pure culture. IOP Conf. Series: Earth and Environmental Science, 140, 012-055.
 
[21]  Ellis, M. B. (1971). Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew, Surrey, England. 464-497.
 
[22]  Simmons, E. G. (2007). Alternaria. An Identification Manual. In: Biodiversity No 6. CBS fungal diversity centre, Utrecht, The Netherlands.
 
[23]  Al-Samarrai, G., Singh, H. & Syarhabil, M. (2012). Evaluating eco-friendly botanicals as alternatives to synthetic fungicides. Annals of Agriculture and Environ. Medicine, 9, 673-676.
 
[24]  Goufo, P., Fontem, A. & Ngnokam, D. (2010). Evaluation of plant extracts for tomato late blight in Cameroon. NZ. J. Crop Hortic. Sci. 38, 171-176.
 
[25]  Grover, R. K. & Moore, J. D. (1962). A toximetric study of fungicides against brown rot organisms Sclerotinia fructicola and S. laxa. Phytopathol. 52, 876-880.
 
[26]  Francisco, D. H. (2010). Lippia graveolens and Carya illinoensis Organic Extracts and there in vitro Effect against Rhizoctonia Solani Kuhn. American Journal of Agricultural and Biological Sciences, 5 (3), 380-384.
 
[27]  Biswas, M. K. & Tanmay, G. (2018). Evaluation of Phyto-extracts, Biological Agents and Chemicals against the Development of Alternaria brassicae in vitro and in vivo European Journal of Medicinal Plants, 22(3), 1-9.
 
[28]  McKinny, H. H. (1923). A new system of grading plant diseases. Agric. Res. 26, 95-98.
 
[29]  Vasudha, A., Kadam, D. N., Dhutraj, D. V. P. & Patil, D. D. (2018). Bio Efficacy of Bio Agents and Botanicals against Alternaria alternata (Fr.) Keissler Causing Leaf Spot of Pomegranate. Int. J. Curr. Microbiol. App. Sci. 7(11), 1146-1155.
 
[30]  Sajad, A. M., & Abid, H. Q. (2017). Antifungal activity of Zingiber officinale oil against plant pathogenic fungi isolated from solanaceous vegetable fruits. Asian Journal of Pharmacy and Pharmacology, 3(4), 121-124.
 
[31]  Bansod, S. & Rai, M. (2008) Antifungal activity of essential oils from Indian medicinal plants against human pathogenic Aspergillus fumigatus and Aspergillus niger. World Journal of Medical Sciences, 3(2):81-88.
 
[32]  Rahmah, A. N., Mostafa, A. A., Abdel-Megeed, A., Yakout, S. M. & Hussein, S. A. (2013). Fungicidal activities of certain methanolic plant extracts against tomato phytopathogenic fungi. Afr. J. Microbiol Res., 7, 517-524.
 
[33]  Olubunmi, A. A., Felix, A. A., Daniels, J. A., Oloswol, O. O. & Ayodele, B. O. (2018). Phytochemical screening and antifungal activities of Zingiber officinale on mycotoxygenic fungi associated with the deterioration of Pennisetum glaucume grains. Journal of Advances in Microbiology, 13(1), 1-11.
 
[34]  Mamarabadi, M., Abbas, T. & Younes, R. (2018). Antifungal activity of recombinant Thanatin in Comparison with two plant extracts and a chemical mixture to control fungal plant pathogens. Open Access.
 
[35]  Wongkaew, P. & Sinsiri, W. (2014). Effectiveness of ringworm cassia and turmeric plant extracts on growth inhibition against some important plant pathogenic fungi. Am. J. Plant Sci., 5, 616- 626.
 
[36]  Chethana, B. S., Ganeshan, G., Rao, A. S. & Bellishree, K. (2012). In-vitro evaluation of plant extracts, bioagents and fungicides against Alternaria porri causing purple blotch diseases of onions, Pest Management in Horticultural Ecosystems, 18 (2), 194-198.
 
[37]  Madhu, G., Sushil, S. & Rekha, B. (2017). Phytotoxicity of Momordica Charantia extracts against Alternaria Alternata. J. Pharm. Sci. & Res. 9 (1), 28-34.
 
[38]  Mudyiwa, R. M., Chiwaramakanda, S., Manenji, B. T. & Takawira, T. (2016). Anti-Alternaria solani Activity of Onion (Allium cepa), Ginger (Zingiber officinale) and Garlic (Allium sativum) in vitro. International Journal of Plant & Soil Science, 10(4), 1-8.
 
[39]  Neeta, S., Richa, T. & Madhu, P. S. (2013). Zingiber officinale Roscoe. Oil: A preservative of stored commodities against storage mycoflora Int. J. Curr. Microbiol. App. Sci, 2(7), 123-134.
 
[40]  Grzanna, R., Lindmark, L. & Frondoza, C. G. (2005). Ginger an herbal medicinal product with broad anti-inflammatory actions. J. Medical. Food, 8, 125-132.
 
[41]  Odhiambo, A. J., Siboe, G. M., Lukhoba, C. W. & Dossaji, F. S. (2009). Antifungal activity of crude extracts of selected medicinal plants used in combination in Lake Victoria basin, Kenya. Plant Product Res J. 13, 35-43.
 
[42]  Mahlo, S. M., Chauk, H. R., McGaw, L. J. & Eloff, J. N. (2013). Antioxidant and antifungal activity of selected plant species used in traditional medicine. J. Med Plants Res. 7(33), 2444-2450.
 
[43]  Bandor, H., Hijazi, A., Ramma, H., Hachem, A., Saad, Z. & Badran, B. (2013). Techniques for the extraction of bioactive compounds from labanese Urticadiotica. Journal of Phytomedicine Clinical Therapeutics, 1(6), 507-513.
 
[44]  Brusotti, G., Cesain, I., Dentamaro, A., Caccialanza, G. & Massolin, G. (2013). Isolation and characterization of bioactive compounds from plant resources: the role of analysis in the ethnopharmacological approach. J. Pharm Biomed Anal. 87, 218-228.
 
[45]  Sumitra, A., Kanojia, A. K., Kumar, A., Mogha, N.& Sahu, V. (2012). Biopesticide formulation to control tomato lepidopteran pest menace. Curr Sci. 102 (7), 1051-1057.
 
[46]  Dabur, R., Gupta, A, Mandal, T. K., Singh, D. D., Bajpai, V., … & Guvar, A. M. (2007). Antimicrobial activity of some Indian medicinal plants. Afr. J. Tradit. Complement Altern. Med. 4 (3), 313-318.
 
[47]  Javaid, A. & Rehman, A. H. (2011). Antifungal activity of leaf extracts of some medicinal trees against Macrophomina phaseolina. J. Med Plants Res. 5(13), 2858-2872.