Enhancement of Plant Growth in Tomato by Inoculation with Plant Growth Promoting Bacillus spp

Kavitha Ramavath¹, Bee Hameeda¹ and Gopal Reddy^1,

¹Department of Microbiology, University College of Science, Osmania University, Hyderabad 500 007

Cite this paper:
Kavitha Ramavath, Bee Hameeda and Gopal Reddy. Enhancement of Plant Growth in Tomato by Inoculation with Plant Growth Promoting Bacillus spp. World Journal of Agricultural Research. 2019; 7(2):69-75. doi: 10.12691/wjar-7-2-5

Abstract

Four bacterial isolates selected from among 200 obtained from different source samples were evaluated for plant growth promoting (PGP) traits. These are found to be good for P solubilization, IAA, HCN, siderophore and NH₃ production with antifungal activity on phytopathogenic fungi and abiotic stress tolerance. Tomato plant growth was enhanced by these isolates at seed germination (14-19%) and pot culture (increase in biomass 47-76%). These isolates are identified as Bacillus siamensis RS8, Bacillus tequilensis MS3, Bacillus subtilis subsp stercoris MS19 and Bacillus velezensis MS20 having potential for developing as bioinoculants to enhance the tomato plant growth and productivity.

Keywords:
PGPB PGPR Bacillus spp bio-inoculants tomato plant

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References:

[1]	Al-Amri, S.M., “Improved growth, productivity and quality of tomato (solanum lycopersicum L) plants through application of shikimic acid,” Saudi Journal of Biological sciences, 20(4), 339-345. October 2013.
[2]	Jacoby, R., Peukert. M., Succurro, A., Koprivova, A., and Kopriya. S., The role of soil microorganisms in plant mineral nutrition-Current knowledge and future directions, Frontiers in Plant science, 8. 1617. September 2017.
[3]	Santoyo, G., Orozco-Mosqueda, M.D.C., Govindappa, M., “Mechanisms of biocontrol and plant growth promoting activity in soil bacterial species of Bacillus and Pseudomonas: A review,” Biocontrol Science and Technology, 22(8). 855-872. May 2012.
[4]	Dutta, S., Podile, A.R., Plant growth promoting rhizobacteria (PGPR): the bugs to debug the root zone, Critical Reviews in Microbiology, 36(3). 232-244. August 2010.
[5]	Bashan, Y., de-Bashan, L.E., Prabhu, S.R., and Hernandez, J.P., “Advances in plant growth-promoting bacterial inoculant technology: Formulations and practical perspectives (1998-2013),” Plant and Soil, 378. 1-33. May 2014.
[6]	Gupta, G., Parihar, S.S., Ahiwar, N.K., Snehi, S.K., and Sing, V., “Plant growth promoting Rhizobacteria (PGPR): Current and Future Prospects for Development of Sustainable Agriculture,” Journal of Microbial Biochemical Technology, 7(2). 096-102. March 2015.
[7]	Calvo, P., Nelson, L., and Kloepper, J.W., “Agricultural uses of plant biostimulants,” Plant and Soil, 383(1-2). 3-41. October 2014.
[8]	Gouda, S., Kerry, R.G., Das, G., Paramithiotis, S., Shin, H.S., and Patra, J.K., “Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture,” Microbiological Research, 206. 131-140. October 2017.
[9]	Nautiyal, C.S., “Efficient microbiological growth medium for screening phosphate solubilizing microorganisms,” FEMS Microbiology Letters, 170(1), 265-270. January 1999.
[10]	Gordon, S.A., and Weber, R.P., “Colorimetric estimation of indoleacetic acid,” Plant physiology, 26(1). 192-195. January 1951.
[11]	Schwyn, B., Neilands, J.B., “Universal Chemical assay for the detection and determination of siderophores,” Analytical Biochemistry, 160(1). 47-56. January 1987.
[12]	Vaikuntapu, P.R., Dutta. S., Samudrala, R.B. et.al s “Preferential Promotion of Lycopersicon esculentum (Tomato) Growth by Plant Growth Promoting Bacteria Associated with Tomato,” Indian Journal of Microbiology, 54(4). 403-412. December 2014.
[13]	OToole, G.A., Kolter, R., “Flagellar and Twitching Motility Are Necessary for Pseudomonas aeruginosa Biofilm Development.” Molecular Microbiology, 30(2). 295-304. July 1998.
[14]	Chari, D.K., Reddy, R.S., Triveni, S., Trimurtulu, N., Rani, C.V.D., and Sreedhar, M., Isolation and Characterization of Abiotic Stress Tolerant Plant Growth Promoting Bacillus spp. From Different Rhizospheric soils of Telangana, Biosciences Biotechnology Research Asia, 15(2). 485-494. June 2018.
[15]	Girish, N., Umesha, S., “Effect of plant growth promoting rhizobacteria on bacterial canker of tomato,” Archives of phytopathology and plant protection, 38(3). 235-243. August 2005
[16]	Kumari, P., Meena, M., and Upadhyay, R.S., “Characterization of plant growth promoting rhizobacteria (PGPR) isolated from the rhizosphere of Vigna radiata (mung bean),” Biocatalysis and Agricultural Biotechnology, 16. 155-162. July 2018.
[17]	Batool, S., Iqbal, A., “Phosphate solubilizing rhizobacteria as alternative of chemical fertilizer for growth and yield of Triticum aestivum (Var. Galaxy 2013),” Saudi Journal of Biological Sciences, May 2018.
[18]	Altaf, M.M., Ahmad I., “Plant Growth Promoting activities, biofilm formation and root colonization by Bacillus spp. Isolated from rhizospheric soils,” Journal of Pure and Applied Microbiology, 10. 109-20. 2016.
[19]	Tank, N., Saraf, M., “Salinity-resistant plant growth promoting rhizobacteria ameliorates sodium chloride stress on tomato plants,” Journal of Plant Interactions, 5(1).51-58. March 2010.
[20]	Akram, T., Aisha Waheed Qurashi., Study of motility and bacterial interactions during biofilm formation of two species bacterial consortia developed by Bacillus subtilis, Bacillus cereus or Alkali gene faecalis under salt stress, Pure and Applied Biology, 7. 271-281. March 2018.
[21]	Vurukonda, S.S.K.P., Vardharajula, S., Shrivastava, M., (2016) Enhancement of drought stress tolerance in crops by plant growth promoting,” Microbiological Research, 184. 13-24. March 2016.
[22]	Nautiyal, C.S., Govindarajan, R., Lavania, M., and Pushpangadan P. “Novel mechanisms of modulating natural antioxidants in functional foods: Involvement of plant growth promoting rhizobacteria NRRL B-30488,” Journal of Agricultural and Food Chemistry, 56(12). 4474-4481. May 2008.
[23]	Shafi, J., Tian, H. and Ji, M., “Bacillus species as versatile weapons for plant pathogens.” Biotechnology & Biotechnological Equipment 31(3). 446-459. February 2017.
[24]	Kumar. P., and Dubey, R.C., Maheshwari, D.k., “Bacillus strains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens,” Microbiological Research, 167(8). 493-499. June 2012.
[25]	Cendales, TC, Gonzalez CAR, Cuasquer CPV., Alzate OAT, and Rodríguez AH., Bacillus effect on the germination and growth of tomato seedlings (Solanum lycopersicum L), Acta Biologica Colombiana, 22(1). 37-44. 2017
[26]	Pishchik. V.N., Vorobyev. N.I., Ostankova, Y.V., and Semenov, A.V., Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer, International Journal of Plant & Soil Science, 22(6). 1-12. May 2018.
[27]	Choudhary, D.K., Sharma, K.P., and Gaur, R.K. Biotechnological perspectives of microbes in agro-ecosystems, Biotechnology Letters, 33(10). 1905-1910. October 2011.
[28]	Toyota, K., “Bacillus-related Spore Formers: Attractive Agents for Plant Growth Promotion.” Microbes Environments, 30(3). 205-207. September 2015.
[29]	Dey, R., Pal, K.K., Bhatt, D.M., and Chauhan, S.M., Growth promotion and yield enhancement of peanut (Arachis hypogea L.) by application of plant growth- promoting rhizobacteria, Microbiological Research, 159(4). 317-394. August 2004.