World Journal of Analytical Chemistry
ISSN (Print): 2333-1178 ISSN (Online): 2333-1283 Website: http://www.sciepub.com/journal/wjac Editor-in-chief: Raluca-Ioana Stefan-van Staden
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World Journal of Analytical Chemistry. 2018, 6(1), 4-12
DOI: 10.12691/wjac-6-1-2
Open AccessArticle

Assessment of Organophosphorus and Pyrethroid Pesticide Residues in Lactuca sativa L. and Solanum macrocarpum L. cultivated in Benin

Prudence Agnandji1, Lucie Ayi-Fanou1, , Magloire Acakpo Nonvignon Gbaguidi2, Boris Fresnel Cachon3, Mathieu Hounha1, Micheline Agassounon Djikpo Tchibozo4, Winfred Seth Kofi Gbewonyo5, Fabrice Cazier6 and Ambaliou Sanni1

1Unité de Biochimie et Biologie Moléculaire, Laboratoire de Biologie Moléculaire et Environnement, Département de Biochimie et Biologie Cellulaire, Université d'Abomey-Calavi 04BP0320 Cotonou, Bénin

2Unité de Recherche en Ecotoxicologie et Etude de Qualité (UREEQ), Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA), Ecole Polytechnique d’Abomey-Calavi (EPAC), Université d’Abomey-Calavi (UAC) 01BP2009 Cotonou, Bénin

3Faculté des Sciences et Techniques de Dassa-Zoume, Université des Sciences, Technologie, Ingénierie et Mathématiques, Dassa-Zoume, Bénin

4Laboratoire des Normes et de Contrôle de Qualité Microbiologique, Nutritionnelle et Pharmacologique, Université d’Abomey-Calavi, 01 BP 1636 RP, Cotonou, Bénin

5Department of Biochemistry, Cell and Molecular Biology, School of Biological Sciences, College of Basic and Applied Sciences, University of Ghana, P.O. Box LG54, Legon

6Centre Commun de Mesures, Université du Littoral Côte d'Opale Dunkerque, France

Pub. Date: April 28, 2018

Cite this paper:
Prudence Agnandji, Lucie Ayi-Fanou, Magloire Acakpo Nonvignon Gbaguidi, Boris Fresnel Cachon, Mathieu Hounha, Micheline Agassounon Djikpo Tchibozo, Winfred Seth Kofi Gbewonyo, Fabrice Cazier and Ambaliou Sanni. Assessment of Organophosphorus and Pyrethroid Pesticide Residues in Lactuca sativa L. and Solanum macrocarpum L. cultivated in Benin. World Journal of Analytical Chemistry. 2018; 6(1):4-12. doi: 10.12691/wjac-6-1-2

Abstract

Pesticides are harmful and toxic in nature. They are used for vegetable protection against pest attack. The present study aimed to determine the level of Organophosphorus and Pyrethroid residues in vegetables (Solanum macrocarpum L. and Lactuca sativa L.). Thirty one vegetable samples (16 samples of Solanum macrocarpum L. and 15 samples of Lactuca sativa L.) from four vegetable farms were collected. The samples were cleaned up before the extraction was performed. Analysis was then processed using the QuEChERS method. Residue analysis was performed using a GC-PFPD for Organophosphorus residues and GC-ECD for Pyrethroid residues. MedCalc Statistical Software version 15.0. was used for the statistical analysis. The results revealed that most of the samples are contaminated by Pyrethroid and Organophosphorus residues. Among Pyrethroid residues detected, 6.5% of cypermethrin and 43.75% of lambda cyhalothrin levels in Solanum macrocarpum L. have exceeded the maximum residue limits while in Lactuca sativa L. 40% of lambda cyhalothrin and 20% of fenvalerate levels exceeded also the maximum residue limits. For Organophosphorus analyzed, 6.25% of diazinon, 12.5% of chlorfenvinp, 18.75% of fenitrothion, 25% of dimethoate and pirimiphos methyl, 50% of profenofos, 56.25% of malathion and 75% of chlorpyrifos levels in Solanum macrocarpum L. exceeded maximum residue limits while 13.33 % of ethoprofos and chlorfenvinp, 20% of profenofos and fenitrothion, 26.67 % of methamidophos, dimethoate and pirimiphos-methyl, 46.67% of malathion and 53.33% of chlorpyrifos levels in Lactuca sativa L. were exceeded the maximum residue limits. No significant difference was observed between the level of Pyrethroid and Organophosphorus residues which are above maximum residue limits in Solanum macrocarpum L. and Lactuca sativa L. The levels of Pyrethroid and Organophosphorus detected in vegetable samples show that the sanitary qualitary of these vegetables is affected.

Keywords:
levels contamination Organophosphorus Pyrethrinoid vegetable crops south of Benin

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References:

[1]  Gregory, P. J., Johnson S. N., Newton A. C., Ingram J. S. I. (2009). Integrating pests and pathogens into the climate change/food security debate. Journal of Experimental Botany, 60: 2827-2838.
 
[2]  Azandeme-Hounmalon G. Y., Affognon, H. D., Assogba-komlan, F., Tamo, M., Fiaboe, K. K. M., Kreiter, S., Martin, T. (2014). Comportement des maraîchers face à l’invasion de Tetranychus Evansi Baker & Pritchard au Sud du Benin. AFPP-Dixième conférence internationale sur les ravageurs en Agriculture Montpellier-22 et 23 Octobre 2014.
 
[3]  Adjrah, Y., Dovlo, A., Karou1, S. D., Eklu-Gadegbeku, K., Agbonon, A., de Souza, C., Gbeassor, M. (2013). Survey of pesticide application on vegetables in the Littoral area of Togo. Annals of Agricultural and Environmental Medicine, 20(4), 715-720.
 
[4]  Gaupp-Berghausen, M., Hofer, M., Rewald, B., Zaller, J. G. (2015). Glyphosate-based herbicides reduce the activity and reproduction of earthworms and lead to increased soil nutrient concentrations. Scientific Reports 5: 12886.
 
[5]  Zakharov, S., Csomor, J., Urbanek, P., Pelclova, D. (2016). Toxic Epidermal Necrolysis After Exposure to Dithiocarbamate Fungicide Mancozeb. Basic Clin Pharmacol Toxicol. 118(1): 87-91.
 
[6]  Sun, X. (2015). History and Current Status of Development and Use of Viral Insecticides in China. Viruses 7: 306-319.
 
[7]  Elliott, M. N.F., Potter, J. C. (1978). The future of Pyrethroids in insect control. Ann. Rev. Entomol. 23:443-69.
 
[8]  Ahouangninou, C. A., Martin, T., Edorh, P., Siddick, I. A., Bio-Bangana, S., Dion, S., Onil, S, S., Boko M., Simon, S., Fagnomi, B.E. (2012). Characterization of health and environmental risks of pesticide used in market-gardening in the rural city of Tori-Bossito in Benin, west Africa. J Environ Prot.
 
[9]  Grzywacz, D., Rossbach, A., Rauf, A., Russell, D.A., Srinivasan, R., Shelton, A.M. (2010). Current control methods for diamondback moth and other brassica insect pests and the prospects for improved management with lepidopteran-resistant Bt vegetable brassica in Asia and Africa. Crop protection 29: 68-79.
 
[10]  Zawiyah, S., Che Man Y.B., Nazimah, S.A.H., Chin, C.K., Tsukamoto, I., Hamanyza, A.H., Norhaizan, I. (2007). Determination of organochlorine and Pyrethroid pesticides in fruit and vegetables using SAX/PSA clean-up column. Food Chem. 102: 98-103.
 
[11]  Agnandji, P., Ayi-Fanou, L., Gbaguidi, A.N.M., Cachon, B.F., Hounha, M., Tchibozo, D.M., Cazier, F., Sanni, A. (2018). Distribution of Organochlorine Pesticides Residues in Solanum macrocarpum and Lactuca sativa Cultivated in South of Benin (Cotonou and Seme-kpodji). American Journal of Food Science and Technology. 6(1): 19-25.
 
[12]  EU. “EU-Pesticides Database”, 2015.
 
[13]  Akomea-Frempong, S., Ofosu, I. W., Owusu-Ansah E. J., Darko, G. (2017). Health risks due to consumption of pesticides in ready-to-eat vegetables (salads) in Kumasi, Ghana. International Journal ofFoodContamination. 4:13.
 
[14]  Quintero, A., Caselles M J., Ettiene, G., Colmenares, N. G., Ramı´rez, T., Deisy, Medina. (2008). Monitoring of Organophosphorus Pesticide Residues in Vegetables of Agricultural Area in Venezuela. Bull Environ Contam Toxicol. 81: 393-396.
 
[15]  Sapbamrer, R., Hongsibsong, S. (2014). Organophosphorus Pesticide Residues in Vegetables From Farms, Markets, and a Supermarket Around Kwan Phayao Lake of Northern Thailand. Arch Environ Contam Toxicol 67: 60-67.
 
[16]  Wanwimolruk, S., Kanchanamayoon, Onnicha., Phopin, K., Prachayasittikul, V. (2015). Food safety in Thailand 2: Pesticide residues found in Chinese kale (Brassica oleracea), a commonly consumed vegetable in Asian countries. Science of the Total Environment 532 :447–455.
 
[17]  Mandal, K., Singh, B. (2010). Magnitude and Frequency of Pesticide Residues in Farmgate Samples of Cauliflower in Punjab, India. Bull Environ Contam Toxicol 85:423-426.
 
[18]  Akoto, O., Gavor, Sandra., Appah, M. K., Apau, J. (2015). Estimation of human health risk associated with the consumption of pesticide-contaminated vegetables from Kumasi, Ghana. Environ Monit Assess 187: 244.
 
[19]  Bempah, C. K., Buah-Kwofie, A., Denutsui, D., Asomaning, J., Tutu, A. O. (2011) .Monitoring of Pesticide Residues in Fruits and Vegetables and Related Health Risk Assessmentin Kumasi Metropolis, Ghana. Research Journal of Environmental and Earth Sciences 3(6): 761-771.
 
[20]  González-Rodríguez, R. M., Rial-Otero, R., Cancho-Grande, B., Simal-Gándara, J. (2008). Occurrence of fungicide and insecticide residues in trade samples of leafy vegetables. Food Chem. 107(3): 1342-7.
 
[21]  Ahmed, M, T., Greish, S., Ismail, S. M., Mosleh, Y., Loutfy N, M. El Doussouki, A. (2013). Dietary Intake of Pesticides Based on Vegetable Consumption in Ismailia, Egypt. A Case Study» Human and Ecological Risk Assessment: An International Journal.
 
[22]  Diop, A., Diop, Y. M., Thiaré, D. D., Cazier, F., Sarr, S. O., Kasprowiak. A, Landy, D., Delattre, F. (2016). Monitoring survey of the use patterns and pesticides residues on vegetables in the Nyayes zone, Senegal. Chemosphere 144: 1715-1721.
 
[23]  Saha, N., Zaman, M. R. (2012). Evaluation of possible health risks of heavy metals by consumption of foodstuffs available in the central market of Rajshahi City, Bangladesh. Environmental Monitoring and Assessment.
 
[24]  Shen, L., Xia, B., Dai, X., (2013). Residues of persistent organic pollutant sinfrequently consumed vegetables and assessment of human health risk based on consump tion of vegetables in Huizhou, South China. Chemosphere 93: 2254-2263.
 
[25]  Arias, L. A., Bojaca, C., Ahumada, A.D., Schrevens, E. (2014). Monitoring of pesticide residues in tomato marketed in Bogota, Colombia. Food Control 35:213-217.
 
[26]  Yihua, L., Danyu, S., Shiliang, L., Zhanglin, N., Ming, D., Caifen, Y., Fubin, T. (2016). Residue levels and risk assessment of pesticides in nuts of China. Chemosphere 144: 645-651.