Sustainable Energy
ISSN (Print): 2372-2134 ISSN (Online): 2372-2142 Website: Editor-in-chief: Apply for this position
Open Access
Journal Browser
Sustainable Energy. 2014, 2(1), 25-28
DOI: 10.12691/rse-2-1-5
Open AccessArticle

Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast

Pradipsaha1, , Md. FakhrulAlam1, Ajit Chandra Baishnab1, MaksudurRahman Khan1, 2 and M. A. Islam1

1Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology (SUST), Sylhet, Bangladesh

2Faculty of Chemical and Natural Resources Engineering, University Malaysia Pahang, Gambang, Kuantan, Pahang, Malaysia

Pub. Date: January 24, 2014

Cite this paper:
Pradipsaha, Md. FakhrulAlam, Ajit Chandra Baishnab, MaksudurRahman Khan and M. A. Islam. Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast. Sustainable Energy. 2014; 2(1):25-28. doi: 10.12691/rse-2-1-5


Bio-Ethanol is a renewable; eco-friendly energy source can be produced from bio-mass (hemicelluloses). Pteris (fern) is grown very fast and has not major economic importance but it is a reliable source of hemicelluloses can be converted to bio-Ethanol. In the hydrolysis of hemicelluloses the concentration of glucose and the reaction rate was observed with respect to the different parameters, like pH, temperature, substrate diameter, substrate loading. In this study we found optimal parameters, NH4OH treatment as the best treatment among H2SO4, NH4OH, and NaOH treatment. In addition, pH 7, temperature 35°C, substrate diameter 45μm-63μm, and substrate loading 0.25gm in 100ml working volume was found as optimum operation condition for hydrolysis reaction, which was carried out by pseudomonas sp., isolated from cow dung. In this experiment sugar concentration was measured with UV spectrophotometer using DNS reagent finding the equilibrium time is 72hours for hydrolysis process and the maximum sugar concentration of 1.7625mg/l was achieved. Subsequently we study the fermentation process using yeast to produce Bio-Ethanol from reducing sugar solution obtained from the hydrolysis process on optimum reaction condition and yield the Ethanol concentration 0.333 mg/L, measured with UV spectrophotometer. Furthermore, it was also observed that the activity of yeast was sustain, in the reaction condition (pH = 7, temperature = 25°C), only for 50 hours. In sum, it could be conclude that 0.1332 mg of ethanol can be produce from 1gm of pteris where the conversion of reducing sugar to ethanol is 20% approximately in the optimum reaction condition.

Hemicellulose pteris hydrolysis pseudomonas sp reducing sugar bio-ethanol fermentation

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Omer AM, the Energy Crisis, the Role of Renewable and Global Warming Greener, “Journal of Environment Management and Public Safety,” Vol. 1 (1), 038-070, 2012.
[2]  Suleiman LN, Renewable Energy as a Solution to Nigerian Energy Crisis, “VUAS,” 13-19, 2010.
[3]  Charles. EW, Biomass ethanol: technical progress, opportunities and commercial challenges,Annul. Rev. Energy Environ,” 24:189-226, 1999.
[4]  DoKyoung L, Vance N, Owens AB, Peter J , Composition of Herbaceous Biomass Feedstock, SDSU, “SGINCCR”,07, 2007.
[5]  Jenni R, “Isolation and Characterization of Lignin from steam pre-treated spruce and its utilization to study cellulase adsorption on lignin” University of Helsinki, 2009, 14.
[6]  Mohammad. J.T, and Keikhosro. K, Enzyme biased hydrolysis process for ethanol from lignocellulosic materials: A Review, “” 2(4), 707-738.
[7]  Zheng Y, Zhongli P, Ruihong Z, Overview of biomass pretreatment for cellulosic ethanol Production, “Int J Agric & Biol Eng,” 2(3), 51-68, 2009.
[8]  Michelle LM, Factors Effecting Ethanol Fermentation via Simultaneous Saccharification and Fermentation, Shanghai Jiao Tong University, (2011) 10.
[9]  Alexander D, Richard D, Scott G, Ryan P, Enzymatic Hydrolysis of Cellulosic Biomass for the Production of Second Generation Biofuels,WPI.”May , 2009.
[10]  Kim O, Magnus B and Gunnar L, A short review on SSF – an interesting process option for ethanol production from lignocellulosic feedstock, “Biotechnology for Biofuels” 1-7,2008.
[11]  Cowan, S. T. & Steel, K. J. “Cowan and Steel's Manual for the Identification of Medical Bacteria,” 2nd edn. Revised by S. T. Cowan. Cambridge, UK: Cambridge University Press. 1974.
[12]  MILLER, G.L. “Analytical Chemistry,” 1959, vol. 31, 426-428.
[13]  Adran C, Prifysgol C, “concentration of ethanol in beer and wine, Experimental Handbook,”University of Wales BANGOR, 2006.
[14]  Mark T. H, Arthur E. H., The Effect of Organosolv Pretreatment on the Enzymatic Hydrolysis of Poplar, “Biotechnology and Bioengineering.” Vol.26, 1984.
[15]  Kristensen JB, Enzymatic hydrolysis of lignocellulose, Substrate interactions and high solids loadings, “Forest & Landscape,” Research No. 42-2008, 2.