Mohammad Akbari, Esmaeil Nadaf, Mahmoud Lotfi, Masoud Tohidfar
Research in Plant Sciences. 2013, 1(1), 1-3DOI:
Abstract: Melon (Cucumis melo L.) is one of the most important horticultural crops that fungal diseases are one of factors limiting its production. So, resistant cultivars have particular importance. Chitinase and β-Glucanase are well-known enzymes for increasing plant resistance to fungal pathogens. Thereby, transformation of Khatooni cultivar as the most important melon in Iran was carried out. Gene constructs consisting of the chitinase, β-1, 3 Glucanase, a selector gene (ntpII) and cauliflower mosaic virus (CaMV) 35S promoter were transferred by Agrobacterium. Transgenic plants were regenerated from medium containing kanamycin (25mg/l) cefotaxime (300mg/l) and BAP (1mg/l). The presences of gene constructs were proved by PCR using specific primers. In this study, the efficacy of transgenic shoots in Khatooni cultivar was 2.7 percent.