Research in Plant Sciences
ISSN (Print): 2333-8512 ISSN (Online): 2333-8539 Website: Editor-in-chief: Fathy El-Fiky
Open Access
Journal Browser
Research in Plant Sciences. 2013, 1(3), 58-61
DOI: 10.12691/plant-1-3-2
Open AccessArticle

Induction and Proliferation of in vitro Mass of Callus of Withania somnifera (L.) Dunal

Suraj Raj Adhikari1, and Bijaya Pant1

1Central Department of Botany, Tribhuvan University

Pub. Date: November 25, 2013

Cite this paper:
Suraj Raj Adhikari and Bijaya Pant. Induction and Proliferation of in vitro Mass of Callus of Withania somnifera (L.) Dunal. Research in Plant Sciences. 2013; 1(3):58-61. doi: 10.12691/plant-1-3-2


Withania somnifera (L.) Dunal. commonly known as “Aswhoganda”, belongs to the family Solanaceae is an important medicinal plant and a major source of alkaloids and steroids. Induction of callus was observed from stem explants on Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of growth hormones viz, 6-Benzylaminopurine (BAP), α-Napthalene acetic acid (NAA), 2,4-Dichlorophenoxy acetic acid (2, 4-D) and kinetin. All the hormone supplemented media gave response for callus growth. The callus was compact and yellowish brown color in all culture condition. The best growth of callus was observed in the MS medium supplemented with 0.5 mgL1BAP + 1.5 mgL-1 NAA; followed by MS medium supplement with 0.5 mgL-1 BAP + 0.5 mgL-1 NAA, 0.5 mgL-1 BAP + 2.0 mgL-1NAA and 1.0 mgL-1 BAP + 1.5 mgL-1NAA at 8 weeks. Mass production of callus might be used for production and isolation of secondary metabolites for medicinal propose in W. somnifera.

Withania somnifera phytohormone callus NAA BAP 24-D kinetin

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


Figure of 1


[1]  Saldanha, C.J., Nicolson, D. H. “Flora of Hassan District Karnataka”, India; Amerind Publishing Co. Pvt. Ltd., Lucknow. 1978.
[2]  Vaidyaratnam, P.S. “Indian Medicinal Plants, a compendium of 500 species”, (Warrier.P.K. Nambiar V.P.K, Ramankutty Eds.), PartII, Orient Longman Publications, Hyderabad., 52-55. 1994.
[3]  Abraham, A., Kirson, I., Glotter, E., Lavie, D. A. “A chemotaxonomic study of Withania somnifera (L.) Dunal.” Phytochem., 7. 957-962. 1968.
[4]  Mohammad, Y. K., Aliabbas, S., Kumar, V., Rajkumar, S., “Recent advances in medicinal plant biotechnology.” Indian journal of Biotechnology, 8(1). 9-22. 2009.
[5]  Yamada, Y., Shoyama, Y., Nishioka, I., Kohda, H., Namera, A. and Okamoto, T., “Clonal micropropagation of Gentiana scabra Bunge var. buregeri Maxim. And examination of the homogeity concerning the gentiopicroside content.” Chemical and pharmaceutical Bulletin. 39. 204-220. 1991.
[6]  Hina, T., Ali, S., Asi, M.R., “Appraisal of an important Flavonoid, Quercetin, in callus cultures of Citrullus colocynthis.” International Journal of Agriculture and Biology, 14(4). 528-532. 2012.
[7]  Pierik, R. L. M., “In-vitro culture of higher plants” Martinus Nijhoff Publishers, Dordrecht, The Netherlands, 1987.
[8]  Gibson, D. M., Ketchum, R. E. B., Hirasuna, T. J., “Shuler, M. L., Potential of plant cell culture for Taxol production.” Science and Applications, 1995.
[9]  Jamil el. al., Regeneration of ginger plant from callus culture through organogenesis and effect of CO2 enrichment on the differentiation of regenerated plant.” Biotechnplogy. 6. 101-104. 2007.
[10]  Khan et. al., In-vitro regeneration of garlic through garlic culture. J. Biol. Sci., 4.189-191. 2004.
[11]  Luthfi et. al., “Selection of cell source and the effect of pH and MS macronutrients on biomass production in cell culture of Tongkat ali (Eurycoma longifolia Jack).” Journal of Plant Biotechnol., 5. 131-135. 2003.
[12]  Shukla, D.D., Bhattarai, N., Pant, B., “In vitro Mass Propagation of Withania somnifera (L.) Dunal.” Nepal Journal of Science and Technology 11.101-106. 2010.
[13]  Rani, G., Grover, I.S., “In vitro callus induction and regeneration studies in Withania somnifera. Plant Cell Tiss Org Cult, 57. 23-27. 1999.
[14]  Rani et. al., “Callus induction and plantlet regeneration in Withania somnifera (L.) Dunal. In Vitro Cell. Dev. Biol. Plant 39. 468-474. 2003.
[15]  Ray, S., Jha, S., “Withanolide synthesis in cultures of Withania somnifera transformed with Agrobacterium tumefaciens.” Plant Sci 146. 1-7. 1999.
[16]  Paudel, S., Adhikari, S. R., Pant, B., “Effect of colchicines on production of secondary metabolites from callus of Withania somnifera (L.).” Journal of Nepal Biotechnology, 3(1). 15-18. 2013.
[17]  Sangwan, N., “In vitro Withanolide Production by Withania somnifera L. Cultures.” Z. Naturforsch 63. 409-412. 2008.
[18]  George, E.F., Sherrington, P.D., “Plant propagation by tissue culture Handbook and Dictionary of commercial Laboratories.” Exegetics limited, Eversley, Basingstoke, Hants, England. 1984.
[19]  Sharma, P. K., Singh, G., Dudhe, R. and Singh, S., “Biological activities of Withania somnifera.” Anul of Bio Research 1 (3). 56-63. 2010.
[20]  Silva, M.A.N. and Senarath, W.T.P.S.K., “In vitro mass propagation and greenhouse establishment of Withania somnifera (L.) Dunal (Solanaceae) and comparison of growth and chemical compounds of tissue cultured and seed raised plants.” J.Natn.Sci.Foundation Sri Lanka, 37(4). 249-255. 2009.
[21]  Arumugam, A., Gopinath, K., “Micro propagation and tissue culture of the endangered medicinal plant withania somnifera by the direct shoot and root initiation method,” International Journal of Applied Biology and Pharmaceutical Technology, 2(3). 2011.