Research in Plant Sciences
ISSN (Print): 2333-8512 ISSN (Online): 2333-8539 Website: http://www.sciepub.com/journal/plant Editor-in-chief: Fathy El-Fiky
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Research in Plant Sciences. 2015, 3(1), 18-26
DOI: 10.12691/plant-3-1-4
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Isolation and in vitro Characterization of Fusarium oxysporum f. sp. elaeidis, Causal Agent of Oil Palm (Elaeis guineensis Jacq.) Vascular Wilt

Ntsomboh-Ntsefong Godswill1, 2, , Epoh-Nguea Toussaint3, Madi- Galdima1, Nsimi-Mva Armand4, 2, Ngando-Ebongue Georges Frank1, Kounga Tagne Samuel3, Mpondo Mpondo Emmanuel3 and Dibong Didier5

1Institute of Agricultural Research for Development (IRAD), Specialized Centre for Oil Palm Research of La Dibamba (CEREPAH), P. O. Box 243 Douala, Cameroon

2Laboratory of Genetics and Plant Improvement of the Faculty of Science, University of Yaounde I

3Faculty of medicine and Pharmaceutical sciences (FMSP), Department of Pharmacy-Ethno pharmacology and Applied Botany, University of Douala, Cameroon

4Institute of Agricultural Research for Development (IRAD), S. W. Regional Centre, Ekona-Cameroon

5Department of Plant Biology, Faculty of Science, B. P. 24157, University of Douala, Cameroon

Pub. Date: April 09, 2015

Cite this paper:
Ntsomboh-Ntsefong Godswill, Epoh-Nguea Toussaint, Madi- Galdima, Nsimi-Mva Armand, Ngando-Ebongue Georges Frank, Kounga Tagne Samuel, Mpondo Mpondo Emmanuel and Dibong Didier. Isolation and in vitro Characterization of Fusarium oxysporum f. sp. elaeidis, Causal Agent of Oil Palm (Elaeis guineensis Jacq.) Vascular Wilt. Research in Plant Sciences. 2015; 3(1):18-26. doi: 10.12691/plant-3-1-4

Abstract

Vascular wilt disease caused by the fungus Fusarium oxysporum f. sp. elaeidis (F.o.e) is one of the major diseases of oil palm (Elaeis guineensis Jacq.) in the South-West region of Cameroon. In order to document the F.o.e isolation and characterization procedures, a 3 months study was carried out in palm plantations around IRAD Ekona and in the palm pathology laboratory located in the South-West region of Cameroon. Some 30 isolates of F.o.e and 10 g of infected soil were collected on and around infected oil palm trees from the field and used to appreciate various morphological characters that influence their aptitude to persist in the soil and on the host. The in vitro mycelial growth, sporulation (conidia), growth rate, biomass and the other characteristics as well as the correlation of the isolates in the culture media were recorded. Results showed that the isolates develop very quickly in the Medium for Mycelium (MM) contrary to PDA medium. In MM, the conidial stocks developed and filled the Petri dish in about 3 days contrary to the filamentous fungal stocks which took about 7 days to fill the Petri dish. These results can improve understanding of the procedures used and devise a selection strategy of the antagonistic microbial stocks for a biological control against oil palm vascular wilt disease.

Keywords:
Fusarium oxysporum f. sp. elaeidis oil palm vascular wilt Elaeis guineensis Jacq. in vitro characterization

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