Research in Plant Sciences
ISSN (Print): 2333-8512 ISSN (Online): 2333-8539 Website: http://www.sciepub.com/journal/plant Editor-in-chief: Fathy El-Fiky
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Research in Plant Sciences. 2014, 2(3), 53-66
DOI: 10.12691/plant-2-3-2
Open AccessReview Article

GC-FID Method Development and Validation Parameters for Analysis of Palm Oil (Elaeis guineensis Jacq.) Fatty Acids Composition

Ntsomboh-Ntsefong Godswill1, 2, , Ngando-Ebongue Georges Frank1, Maho-Yalen Josian Edson2, 3, Youmbi Emmanuel2, 4, Bell Joseph Martin2, Ngalle-Bille Hermine2, Tabi-Mbi Kingsley1, 2, Likeng-Li-Ngue Benoit Constant1, 2 and Nsimi-Mva Armand2, 5

1Lipids Analysis Laboratory, IRAD Specialized Centre for Oil Palm Research of La Dibamba, Douala-Cameroon

2Laboratory of Genetics and Plant Improvement, University of Yaounde 1, Department of Plant Biology, Yaounde-Cameroon

3University of Yaounde 1, Higher Teachers’ Training College, Department of Biological Sciences, Yaounde-Cameroon

4Centre Africain de Recherche sur Bananiers et Plantains (CARBAP), Njombé-Cameroun

5Institute of Agricultural Research for Development (IRAD), S. W. Regional Centre, Ekona-Cameroon

Pub. Date: November 01, 2014

Cite this paper:
Ntsomboh-Ntsefong Godswill, Ngando-Ebongue Georges Frank, Maho-Yalen Josian Edson, Youmbi Emmanuel, Bell Joseph Martin, Ngalle-Bille Hermine, Tabi-Mbi Kingsley, Likeng-Li-Ngue Benoit Constant and Nsimi-Mva Armand. GC-FID Method Development and Validation Parameters for Analysis of Palm Oil (Elaeis guineensis Jacq.) Fatty Acids Composition. Research in Plant Sciences. 2014; 2(3):53-66. doi: 10.12691/plant-2-3-2

Abstract

The variety of available techniques and analytical methods applied in laboratories calls for the need of defining the scope, range of application and quality of information obtained using each of them with reference to internationally agreed standards. Of these techniques, Gas Chromatography with Flame Ionization Detector (GC-FID) is most used in fatty acids analysis. Method development and validation is useful for GC and other analytical procedures. This paper outlines the importance of method validation and elaborates on typical validation parameters with special reference to their application in the analysis of fatty acids of palm oil. The first section presents background information on stages in analytical method processing, quantitative and qualitative analysis, GC and its use in the analysis of lipid extracts. Critical components for a method are highlighted such as sampling, sample preparation (FAME derivatization), instrument analysis conditions and method standardization. Validation characteristics like Specificity, Linearity and range, Accuracy, Precision, LOD, LOQ, Robustness, Ruggedness and System suitability are presented in the last section with a brief view on some statistical methods used in method validation. It is hoped that this review will facilitate the work of analysts and the scientific community by enhancing efficient method development and validation which matches international standards.

Keywords:
GC-FID FAME preparation method development validation characteristics fatty acids Elaeis guineensis Jacq. Gas Chromatography

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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