Isolation and Screening of Marine Bacteria Producing Anti-Cancer Enzyme L-Asparaginase

M. Bhargavi^1, and R. Jayamadhuri¹

¹Department of Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati

Cite this paper:
M. Bhargavi and R. Jayamadhuri. Isolation and Screening of Marine Bacteria Producing Anti-Cancer Enzyme L-Asparaginase. American Journal of Marine Science. 2016; 4(1):1-3. doi: 10.12691/marine-4-1-1

Abstract

PURPOSE: The objective of this investigation was to isolation and screening of marine bacteria for L-asparaginase activity. METHODS: Marine bacteria were isolated from water samples obtained from Kerala sea coast in India. The isolates were identified as marine bacteria by microscopical and bio-chemical tests. Production of L-asparaginase was carried out in submerged fermentation media (glycerol asparagine media). RESULTS: Among three marine isolates subjected to screening only one isolate BKJM-VB showed potential L-asparaginase activity. CONCLUSION: The study revealed that marine bacteria may be a potential source of high yield, high substrate specificity L-asparaginase which is an anti-leukemic agent.

Keywords:
marine bacteria asparaginase sub merged fermentation

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Figures

References:

[1]	Dhanamjayam.G and Kann S(2014)-Screening and Characterization of L-Asparaginase producing Streptomyces Isolated from soil samples of Periyar Lake, Kumily. Bio science Discovery, Vol5 (1).
[2]	Dhevagi P, Poorani E(2006). Isolation and characterization of L-Asparaginase from marine actinomycetes. Indian journal of Biotechnology; 5:514-520.
[3]	Kamble KD, Bidwe PR, Muley VY, Kamble LH, Bhadange DG and Musaddiq M(2012)-Characterization of L-Asparaginase producing bacteria from Water, farm and saline soil. Bio science discovery, Vol3(1).
[4]	Manikandan R,CN Pratheeba, Pankaj S and Sah S (2010)-Optimization of Asparaginase production by Pseudomonas aeruginosa Using Experimental Methods. Nature and Science. Vol 8(2): 1-6.
[5]	Mashburn L and Wriston J(1963). Tumor inhibitory effect of L-Asparaginase.
[6]	Maysa EM, Amira MG and Sanaa TE(2010)-Production, Immobilization and Anti-tumor activity of L-Asparaginase of Bacillus sp R36. Journal of American sciences; 6(8):1-6.
[7]	Palaniappan Sivasankar(2013)- Efficient production of L-Asparaginase by marine Streptomyces sps. Isolated from Bay of Bangal, India. African Journal of Microbiology Research; 7(31): 4015-4021.
[8]	Prakasham RS, Hymavathi M, Subba Rao Ch, Arepalli SK,Venkateswara Rao J, Kavin Kennady P, Nasaruddin K, Vijayakumar JB, Sarma PN (2010). Evaluation of antineo-plastic activity of extracellular asparaginase production by isolated Bacillus circulans. Applied Bio-chemistry Biotechnology. 160; 72-80.
[9]	Sabha Mahmoud EI-Sabbagh, Nadia Hamed EI-Batanony and Tarek A.Salem(2013)-L-Asparaginase produced by Streptomyces strain isolated from Egyptian soil:Purification, characterization and evaluation of Anti-tumor. African Journal of Microbiology Research; Vol7(50); 5677-5686.
[10]	Saleem Basha N, Rekha R, Komala M and Ruby S(2009)-Production of Extracellular Anti-leukaemic Enzyme L-Asparaginase from Marine Actinomycetes by Solid-state and Submerged fermentation:Purification and characterization. Tropical Journal of pharmaceutical Research; 8(4): 353-360.
[11]	Selvakumar Dharmaraj (2011). Study of L-Asparaginase production by Streptomyces noursei MTCC 10469, Isolated from marine sponge callyspongia diffusa. 9.
[12]	Shab AJ, Karadi RV and PP Parekh (2010)-Isolation, optimization and Production of L-Asparaginase from Coliform bacteria. Asian Journal of Biotechnology; 2: 169-177.
[13]	Tosa T, Sano R, Yamamoto K, Nakamura M and Chibata I (1972)-L-Asparaginase from Proteus vulgaris: Purification, Crystalization and enzymic properties. Journal of Bio-chemistry; 11: 217-222.