World Journal of Preventive Medicine
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World Journal of Preventive Medicine. 2015, 3(1), 7-10
DOI: 10.12691/jpm-3-1-2
Open AccessArticle

Group B Streptococcal Carriage Rate in Vagina of Pregnant Women in Third Trimester in Lomé, Togo

Salou Mounerou1, 2, , Dagnra Anoumou Y.1, 2, Adama – Hondégla Amah Biova3, Ekouevi Koumavi2, Dossim Sika2, Kao Kpatcha2, Yehadji Degninou2, Nyasenu Y.Tufa1, 2, Akpadza Koffi3 and Prince-David Mireille1, 2

1Service de Microbiologie, CHU Sylvanus Olympio BP 57 Lomé

2Laboratoire de Biologie moléculaire et d’Immunologie (BIOLIM/FSS) Université de lomé BP1515 Lomé

3Service de gynécologie-obstétrique, CHU Sylvanus Olympio BP 57 Lomé

Pub. Date: February 25, 2015

Cite this paper:
Salou Mounerou, Dagnra Anoumou Y., Adama – Hondégla Amah Biova, Ekouevi Koumavi, Dossim Sika, Kao Kpatcha, Yehadji Degninou, Nyasenu Y.Tufa, Akpadza Koffi and Prince-David Mireille. Group B Streptococcal Carriage Rate in Vagina of Pregnant Women in Third Trimester in Lomé, Togo. World Journal of Preventive Medicine. 2015; 3(1):7-10. doi: 10.12691/jpm-3-1-2


Background: Transmission of Group B Streptococci (GBS) to newborns occurs in the perinatal period through direct channels in utero. GBS is one of the main bacteria responsible for neonatal infections. Objective: measure the prevalence of GBS genital carriage among pregnant women in the third trimester at the Sylvanus Olympio University Teaching Hospital. Materials and Methods: Vaginal swabs were obtained from 200 women between 34 and 38 weeks of pregnancy. The samples were seeded on sheep blood agar at 37o C for 16 to 18h. After incubation, suspected GBS colonies were identified by using a Latex Agglutination Test (LAT). Susceptibility test to antibiotics was performed by agar diffusion assay. Results: A total of 200 pregnant women with an average age of 28 years, were screened for GBS infection. The age group of 25-29 year olds was the highest (33.5%). The women as retailers were the majority (36.5%). Regarding the level of education, the percentage was 12.5%, 30.5%, 49% and 8% corresponding to uneducated, primary, secondary and university level respectively. The carriage rate was 2.5% (n = 5/200), 95% CI (0.3-4.7). No risk factors associated with the carriage rate identified. The isolated GBS strains were susceptible to penicillin G, erythromycin, co-amoxiclav and levofloxacin. The five GBS carriers were delivered by cesarean section for various reasons. Conclusion: Although a low carriage (2.5%) rate of GBS found in this study, a policy of systematic screening of pregnant women at least in the third trimester must be promoted.

pregnancy screening group B streptococcus women Latex Agglutination Test

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[1]  Eickhoff TC, Klein, JO, Daly AK et al Neonatal sepsis and other infections due to group B beta-hemolytic streptococci. New England Journal of Medicine, 1964: 271(24): 1221-1228.
[2]  Krohn MA, Sharon L. Hillier, Carol JB Maternal peripartum complications associated with vaginal group B streptococci colonization. Journal of Infectious Diseases.1999, 179(6): 1410-1415.
[3]  Regan JA, Klebanoff M A., Nugent RP et al. Colonization with group B streptococci in pregnancy and adverse outcome. American journal of obstetrics and gynecology. 1996, 174 (4): 1354-1360.
[4]  Stoll BJ and Schuchat A Maternal carriage of group B streptococci in developing countries. Pediatric infectious disease journal.1998, 17(6): 499-503.
[5]  Schrag S, Gorwitz R, Fultz-Butts K, Schuchat, A. Prevention of perinatal group B streptococcal disease MMWR Recomm Rep51.2002, (11): 1-22.
[6]  Lancefield, Rebecca C. A serological differentiation of specific types of bovine hemolytic streptococci (group B). J Exp Med 1934;(59): 441-448.
[7]  Baker CJ, Clark DJ, Barrett FF Selective broth medium for isolation of group B streptococci. Applied microbiology. 1973, 26(6): 884-885.
[8]  Maxted, Wr R. Preparation of streptococcal extracts for Lancefield grouping. The Lancet. 1948, 252(6520): 255-256.
[9]  Bonnet R, Cavallo JD, Chardon H et al. Comité de l'antibiogramme de la Société Française de Microbiologie.2012, Recommandations 2011. Société Française de Microbiologie.
[10]  Verani, Jennifer R., Lesley McGee, and Stephanie J. Schrag Prevention of perinatal group B streptococcal disease. Morbidity and Mortality Weekly Report (MMWR), Revised Guidelines from CDC, Recommendations and Reports 59.RR10 (2010): 1-32.
[11]  ANAES : Prévention anténatale du risque infectieux bactérien néonatal précoce. ANAES, Service Recommandations et Références Professionnelles. Septembre 2001. IBSN 2-910653-00-0.
[12]  David-Prince M, Ategbo S, De Souza A E, Eklu-Avlassu EK, Grunitzky-Bekele M, Schmidt-Ehry G, Hodonou K, et. Assimadi K. Portage du streptocoque B dans le couple mère-enfant à la naissance: a propos de 106 cas. Bulletin de la Société de pathologie exotique. 1991, 84(5-5BIS): 522-531.
[13]  Nsagha, D.S.; Bello, C.S.S. and Kahdakai- Olukemi, V.T. Maternal Carriage in Pregnancy of Group B Streptococcs in Jos: Relation of Endocervical Anorectal Colonization. Nig. Qt. J. Hosp. Med. 1997, 7: 53-56.
[14]  Nwachukwu, N.C.; Utsalo, S. J.; Kanu, I. and Anyanwu, E.C. Genital Colonization of Group B Streptococcus at Term Pregnancy in Calabar, Nigeria. The Internet Journal of Pediatrics and Neonatology.2007. ISSN: 1528-8374.
[15]  Onile, B.A. Group B Streptococcus carriage in Nigeria. Trans. Rovy. Sco. Trop. Med. & Hyg. 1980: 74: 367-370. Onile 1980 (13).
[16]  Uhiara, J.E. Group B Streptococcal carriage among parturient and their neonates in Zaria, Nigeria. Afr. J. Med. 1993 Sci. 22(3): 79-83.
[17]  Donbraye-Emmanuel, O.O.B.; Okonko, I.D.; Donbraye, E.; Fadeyi, A.; Abubakar, M.J, Adebiyi, O.E. and Fashina, N.A. Isolation and characterization of Group B Steptococci and other pathogens among pregnant women in Ibadan Southwest Nigeria. Journal of Applied Biosciences. 2010, 29: 1781-1792.
[18]  Onipede A., Adefusi O, Adeyemi A., Adejuyigbe E., Oyelese A., Ogunniyi*T. Group B streptococcus carriage during late pregnancy in ile-ife, Nigeria. Afr. j. cln. exper. microbiol. 2012, 13(3): 135-143.
[19]  Kacou, A, Faye-kette A H, Sylla-koko FD et al. Distribution des streptocoques dans les produits biologiques analysés à Abidjan de 1982 à 1988. Médecine d'Afrique noire. 1991, 38 (7): 538-540.
[20]  Suara, RO, Adegbola RA, Baker C J et al. Carriage of group B streptococci in pregnant Gambian mothers and their infants. Journal of Infectious Diseases. 1994, 170(5): 1316-1319.
[21]  Moyo, S.R.; Modzori, J.; Tswana, S.A.; Mealand, J.A. Prevalence, capsular type distribution, authropometric and obstetric factors of group B Streptococcus (Streptococcus agalactiae) colonization in pregnancy. Central Africa Journal of Medicine. 2000: 40(5): 115-120.
[22]  Dzowela, T.; Komolafe, O.O. and Igbigbi, A. Prevalence of Group B Streptococcus colonization in antenatal women at the Queen Elizabeth Central Hospital, Blantyre,a preliminary study. Malawi Medical Journal. 2005. 17 (3): 97-99.
[23]  Ferjani A, Abdallah H B., Saida NB et al. Portage vaginal de Streptococcus agalactiae chez la femme enceinte en Tunisie: facteurs de risque et sensibilité aux antibiotiques des isolats. Bull Soc Pathol Exot. 2006, 99 (2): 99-102.
[24]  Barcaite E, Bartusevicius A, Tameliene R, Kliucinskas M, Maleckiene L, Nadisauskiene R.Prevalence of maternal group B streptococcal colonisation in European countries. Acta Obstet. Gynecol. Scand. 2008, 87: 260-271.
[25]  Jones N, Oliver K., Jones Y et al., Carriage of group B streptococcus in pregnant women from Oxford, UK. Journal of clinical pathology.2006, 59 (4): 363-366.
[26]  El Aila NA, Tency I, Claeys G, Saerens B, Cool P, Verstraelen H, Temmerman M, Verhelst R, Vaneechoutte M. Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women BMC Infectious Diseases 2010, 10: 285
[27]  Kavitha PK, Shrikala B, Suchithra S, Bharati B. Evaluation of Culture, Antigen Detection and Polymerase Chain Reaction for Detection of Vaginal Colonization of Group B Streptococcus (GBS) in Pregnant Women Journal of Clinical and Diagnostic Research. 2014 Feb, Vol-8 (2): 47-49.
[28]  Turner C, Turner P, Po L, Maner N, De Zoysa A, Baharak Afshar, Androulla Efstratiou, Paul T Heath and François Nosten. Group B streptococcal carriage, serotype distribution and antibiotic susceptibilities in pregnant women at the time of delivery in a refugee population on the Thai-Myanmar border turner et al. BMC Infectious Diseases 2012, 12: 34
[29]  Abdolkarim Hamedi, Farideh Akhlaghi, Seyed Javad Seyedi, and Abdolali Kharazmi. Evaluation of Group B Streptococci Colonization Rate in Pregnant Women and Their Newborn Acta Medica Iranica. 2012: 50, 12.
[30]  Paulo Cesar Giraldo EdilsonD.Araujo,Jose ́ Eleute ́rio Junior, Rose Luce Gomes do Amaral, Mauro R. L. Passos, and Ana Katherine Gonc ̧alves The Prevalence of Urogenital Infections in
Pregnant Women Experiencing Preterm and Full-Term Labor.
[31]  Infectious Diseases in Obstetrics and Gynecology 2012, Article ID 878241, 4 pages.
[32]  Chohan, L.; Hollier, L.M.; Bishop, K. and Kilpatrick, C.C. Patterns of antibiotic resistance among group B streptococcus isolates: 2001-2004. Infect. Dis. Obstet. Gynecol. 2006, 57492.
[33]  Gupta C, Briski LE: Comparison of two culture media and three sampling techniques for sensitive and rapid screening of vaginal colonization by group B streptococcus in pregnant women. J Clin Microbiol 2004, 42(9): 3975-3977.
[34]  Trappe KL, Shaffer LE, Stempel LE. Vaginal-perianal compared with vaginal-rectal cultures for detecting group B streptococci during pregnancy. Obstet Gynecol. 2011, 8:313-317.
[35]  El Aila N.A, Tency I, Claeys G, Saerens B, De Backer E, Temmerman M, Verhelst R and Vaneechoutte M. Genotyping of Streptococcus agalactiae (group B Streptococci) isolated from vaginal and rectal swabs of women at 35-37 weeks of pregnancy
 BMC Infectious Diseases 2009, 9: 153.
[36]  Rallu F, Barriga P, Scrivo C, Martel – Laferriere V, Laferriere C. Sensitivities of antigen detection and PCR assays greatly increased compared to that of the standard culture method for screening for Group B Streptococcus carriage in pregnant women. J Clin Microbiol. 2006, 44: 725-28.
[37]  Werneke M, Mullen C, Sharma V, Morrison J, Barry T, Maher M et al. Evaluation of a novel Real-time PCR test based on ssrA gene for the identification of Group B Streptococci in vaginal swabs. BMC Infect Dis. 2009; 9: 148.
[38]  Winn W, Allen S, Janda W. Koneman’s Color Atlas and Text book of Diagnostic Microbiology. 6th edition. 2006; 683-717.
[39]  Rachel M. Ostroff and Jeffrey W. Steaffens. Effect of Specimen Storage, Antibiotics, and Feminine Hygiene Products on the Detection of Group B Streptococcus by Culture and the STREP B OIA Test Diagn Microbiol infect dis. 1995, 22: 25-259.