Journal of Polymer and Biopolymer Physics Chemistry
ISSN (Print): 2373-3403 ISSN (Online): 2373-3411 Website: Editor-in-chief: Martin Alberto Masuelli
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Journal of Polymer and Biopolymer Physics Chemistry. 2018, 6(1), 39-44
DOI: 10.12691/jpbpc-6-1-5
Open AccessReview Article

Molecular Weight Distribution for Biopolymers: A Review

Mohammad R. Kasaai1,

1Department of Food Science and Technology, Sari Agricultural Sciences and Natural Resources University, Khazar Abad road, Km. 9, P.O. Box, 578, Sari, Mazandaran, Iran

Pub. Date: November 21, 2018

Cite this paper:
Mohammad R. Kasaai. Molecular Weight Distribution for Biopolymers: A Review. Journal of Polymer and Biopolymer Physics Chemistry. 2018; 6(1):39-44. doi: 10.12691/jpbpc-6-1-5


In this study, molecular weight distribution (MWD), of polymers with emphasis on MWD of biopolymers, e.g, carbohydrate polymers, proteins, deoxyribonucleic acid, DNA, and ribonucleic acid, RNA, are reviewed. The MWD of biopolymers are compared with those of synthetic polymers. The constitution of a polymer as well as the MWD may be described either by a set of different average molecular weights, the ratios of two different types of average molecular weights, or by the distribution functions via graphical presentation. Polysaccharides in a similar way to synthetic polymers are polydisperse polymers, whereas proteins, DNA, and RNA, are mostly monodisperse macromolecules.

biopolymers molecular weight distribution proteins polysaccharides DNA RNA

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[1]  Alger, M. Polymer Science Dictionary, Chapman & Hall, London, 1999.
[2]  den Hollander., F. Random Polymers. Springer-Verlag: Berlin, 2009.
[3]  Grosberg, A. Y.,and Khokhlov. A.R. Statistical Physics of Macromolecules, AIP Press: New York, 1994.
[4]  Berg, J.M., Tymoczko.,J. L.,and Stryer. L. Biochemistry, 5th ed W.H. Freeman and Company: New York , 2002.
[5]  IUPAC. Compendium of Polymer Terminology and Nomenclature RSC Publishing: Cambridge (U.K), 2008.
[6]  L¨®pez-L¨®pez, E.A., Hern¨¢ndez-Gallegos, M.A., Cornejo-Maz¨®n, M., and Hern¨¢ndez- S¨¢nchez, H. "Polysaccharide-based nanoparticles" In: Food Nanoscience and Nanotechnology H. Hernandez-Sanchez, and G.F. Gutierrez-Lopez, eds Springer: New York, 2015, pp.59-68.
[7]  Stilverthorn, D.U., Johnson, B.R., Ober, W.C., Garrison, C.W., and Stiverthorn, A.C. .Human Physiology: An Integrated Approach, 5thed., Pearson: Benjamin Cummings: San Francisco, 2009.
[8]  McMurry. J. Organic Chemistry, 5th ed Brooks/ Cole Thomson Learning: Boston, 2000, Chapter.28 (pp. 1150- 1192).
[9]  Mandelkern. L. An Introduction to Macromolecule, 2nd ed Springer-Verlag: New York, 1983.
[10]  IUPAC. Compendium of Chemical Technology 2nd ed (the Gold Book), RSC Publishing: Cambridge (UK), 1997.
[11]  Mandal, B.M. Fundamentals of Polymerization, World Scientific: New Jersey, 2013, C1 (pp.1-35).
[12]  Rabek, J.F. Experimental Methods in Polymer Chemistry, John Wiley & Sons: New York, 1980.
[13]  Charraher, C.F. Introduction to polymer chemistry, CRC, Taylor & Francis: Boca Raton, 2007.
[14]  Flory, P.J. Principles of Polymer Chemistry, Cornell University Press, Ithaca: New York, 1953.
[15]  Berek, D. "Molecular characterization of synthetic polymers by means of liquid chromatography" In: Physical chemistry of macromolecules C.H. Chan, C.H. Chia, S. Thomas, eds. CRC Press, Taylor and Francis: Boca Raton, 2014, pp. 223-331.
[16]  Denuzi¨¨re, A., Yagoubi, N., Baillet, A., and Ferrier, D. "An improved statistical parameter allowing elaborate comparison of polymer molecular weight distribution by gel filtration chromatography: Application to chitosan" S.T.P. Pharma Sci., 1995, vol. 5, no. 6, pp. 481-485.
[17]  Freeman, W.F. "Characterization of polymers" In: Encyclopedia of polymer Science and Engineering. H.F. Mark et al. eds. John Wiley & Sons: New York, 1985, vol. 3, pp. 290-327.
[18]  Tager, A. Physical Chemistry of polymers, 2nd ed Mir Publisher: Moscow, 1978.
[19]  Gaborieau, M., and Castignolles, P. "Size-exclusion chromatography (SEC) of branched polymers and polysaccharides" Analytical and Bioanalytical Chemestry, 399, (4), 1413-1423, Feb 2011.
[20]  Koningsveld, R. "Preparative and analytical aspects of polymer fractionation". Advances in Polymer Science, Springer: Berlin, 1970, vol. 7, pp. 1-69.
[21]  BillMeyer, F.W., and Sierbert, L.R. "Application of the summative-fractionation method to the determination of Mw/Mn for narrow-distribution polymers". Advances in Chemistry Series, vol. 125, American Chemical Society, Washington: 1973, Chapter. 2 (pp 9-16).
[22]  Fee, C.J., and Van Alstine, J.M. "Prediction of the Viscosity Radius and the Size Exclusion Chromatography Behavior of PEG ylated Proteins" Bioconjugate Chemistry., 15 (6), 1304-1313, Nov 2004.
[23]  Yau, W.W., Kirkland, J.J., and Bly, D.D. Modern Size Exclusion Liquid Chromatography, Wiley Inter-Science: New York, 1979.
[24]  Kasaai, M.R., Arul, J., and Charlet, G. "Intrinsic viscosity-molecular weight relationship for chitosan", Journal of Polym. Science: Part B: Polymer Physics, 38 (19), 2591-2598, Aug. 2000.
[25]  Knaul, J.Z., Kasaai, M.R., Bui, V.T., and Creber, K.A.M. "Characterization of deacetylated chitosan and chitosan molecular weight review", Canadian Journal of Chemistry, 76, (11), 1699-1706, Nov 1998.
[26]  Elias, H.G. Macromolecules. 1: Structure and Properties 2nd ed Plenum Press: New York, 1984, Chapter 8 (pp. 281- 371).
[27]  Janca, J., and Pokorny, S. "Coupling of a gel permeation chromatograph and automatic capillary viscometer: II. Method for the determination of the molecular weight distribution and of the resolution factor" Journal of Chromatography, 134 (2), 273-278, Apr. 1977.
[28]  Charraher, C. E. Polymer Chemistry, 7th ed. CRC, Taylor & Francis: Boca Raton, 2007.
[29]  Kasaai., M.R. "Intrinsic Viscosity¨CMolecular Weight Relationship and Hydrodynamic Volume for Pullulan", Journal of Applied Polym. Science,.100 (6), pp. 4325-4332, Jun 2006.
[30]  Cooley, R.S., and Schweitzer, P.A. "High performance liquid chromatography" In: Handbook of Separation Techniques for Chemical Engineering, 3rded. P.A. Schweitzer, ed. McGraw-Hill, Inc: New York:, 997, pp. 1-669- 1-679.
[31]  Kolpaka, F.J., Brady, J.E., and Fujinari, F.M. The determination of compositional and molecular weight distributions of cationic polymers using chemiluminescent nitrogen detection (CLND) in aqueous size exclusion chromatography" In Developments in Food Science: Instrumental Methods in Food and Beverage Analysis, D.L.B. Wetzel, G. Charalambous, eds. Elsevier: Amsterdam, 1998, vol. 39, pp. 467-473.
[32]  Groycoolea, F.M., and Chronakis, I.S. "Specific methods for the analysis of identity and purity of functional food polysaccharides". In Developments in Food Science. Instrumental Methods in Food and Beverage Analysis, D.L.B. Wetzel, and G. Charalambous, eds. Elsevier: Amsterdam, 1998, vol. 39, pp. 99-140.
[33]  Burinsky, D.J., and Wang, F. "Mass spectral characterization". In: Handbook of Iisolation and Characterization of Impurities in Pharmaceuticals. S. Ahuja, K.M. Alsante, eds., Academic Press: Amsterdam, 2003, pp.249-299.
[34]  Wang, Q., and Cui., S.W."Understanding the physical properties of food carbohydrates" In: Food carbohydrates, S. W. Cui, ed. CRC, Taylor & Francis Group: Boca Raton, 2005, pp. 161-217.
[35]  Kasaai. M.R. "Dilute solution properties and degree of chain branching for dextran". Carbohydrate Polymers, 88 (1), 373-381, March 2012.
[36]  Barrales-Rienda, J.M., Bello, A., Bello, P.,and Guzman, G.M. "Fractionation of polymers". In Polymer Handbook 4th ed J. Brandrup, E. H. Immergut, and E. A. Grulke, eds, Wiley¨CInterscience: New York, 1999, pp. VII/327- VII/ 496.
[37]  Francuskiewicz, F. Polymer Fractionation. Springer: Berlin, 1994.
[38]  Sperling, L.H. Introduction to Physical Polymer Science, John Wiley & Sons: New York, 1992.