Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2018, 6(10), 645-654
DOI: 10.12691/jfnr-6-10-5
Open AccessArticle

Physicochemical, Antioxidant and Antimicrobial Properties of selected Portuguese Commercial Monofloral Honeys

João Gonçalves1, Isabel Ribeiro1, Joana Marçalo1, 2, Patrícia Rijo1, 2, Célia Faustino1 and Lídia Pinheiro1,

1Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto 1649-003 Lisbon, Portugal

2Universidade Lusófona’s Research Center for Biosciences and Health Technologies (CBIOS), 1749-024 Lisbon, Portugal

Pub. Date: November 13, 2018

Cite this paper:
João Gonçalves, Isabel Ribeiro, Joana Marçalo, Patrícia Rijo, Célia Faustino and Lídia Pinheiro. Physicochemical, Antioxidant and Antimicrobial Properties of selected Portuguese Commercial Monofloral Honeys. Journal of Food and Nutrition Research. 2018; 6(10):645-654. doi: 10.12691/jfnr-6-10-5


The physicochemical, antioxidant and antimicrobial properties of selected Portuguese commercial honeys have been characterized aiming at establishing correlations between honey bioactivity and the physicochemical descriptors. All honey samples met the European regulations on honey quality criteria, including moisture and sugar content, free acidity, diastase activity, electrical conductivity, ashes, hydroxymethylfurfural and proline content. Antioxidant activity was evaluated based on phenolic and flavonoid content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP) and oxygen radical antioxidant capacity (ORAC) assays. The honeys showed antibacterial and antifungal activity with minimum inhibitory concentrations (MIC) between 6.25–25% (w/v) and minimum fungicidal concentrations (MFC) in the range 12.5–50% (w/v), respectively. The bioactivity and physicochemical parameters of honey samples were correlated and depended on the honey floral source. The darkest honey, i.e. heather honey, showed the highest antioxidant and antimicrobial activities, which can be attributed to its higher phenolic, flavonoid and protein content.

portuguese honey antioxidant antimicrobial phenolic compounds flavonoids

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