Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2018, 6(9), 557-560
DOI: 10.12691/jfnr-6-9-3
Open AccessArticle

Daidzein Has Anti-Oxidant Activity in Normal Human Kidney Tubular HK-2 Cells via FOXO3/SOD2 Pathway

Jongsung Lee1 and See-Hyoung Park2,

1Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea

2Department of Bio and Chemical Engineering, Hongik University, Sejong, 30016, Korea

Pub. Date: September 18, 2018

Cite this paper:
Jongsung Lee and See-Hyoung Park. Daidzein Has Anti-Oxidant Activity in Normal Human Kidney Tubular HK-2 Cells via FOXO3/SOD2 Pathway. Journal of Food and Nutrition Research. 2018; 6(9):557-560. doi: 10.12691/jfnr-6-9-3

Abstract

The anti-oxidant activity of daidzein was accessed by checking the expression level of superoxide dismutase 2 (SOD2) in HK-2 cell, a kidney proximal tubular cell line. SOD2 expression was increased in HK-2 cells treated by daidzein with a dose and time-dependent manner. Daidzein reduced the reactive oxygen species (ROS) level (about 60% compared to DMSO control with p = 0.005) in HK-2 cells. Daidzein caused FOXO3 to be translocated from cytosol into nucleus. Down-regulation of FOXO3 by transfection of siRNA against FOXO3 attenuated the expression of SOD2 and ROS level (p = 0.006) by daidzein. Taken together, this study has shown that daidzein displays anti-oxidant activity in HK-2 kidney proximal tubular cell line through up-regulation of FOXO3/SOD2 signaling pathway.

Keywords:
daidzein anti-oxidant HK-2 FOXO3 SOD2

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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