Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Journal of Food and Nutrition Research. 2018, 6(9), 557-560
DOI: 10.12691/jfnr-6-9-3
Open AccessArticle

Daidzein Has Anti-Oxidant Activity in Normal Human Kidney Tubular HK-2 Cells via FOXO3/SOD2 Pathway

Jongsung Lee1 and See-Hyoung Park2,

1Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea

2Department of Bio and Chemical Engineering, Hongik University, Sejong, 30016, Korea

Pub. Date: September 18, 2018

Cite this paper:
Jongsung Lee and See-Hyoung Park. Daidzein Has Anti-Oxidant Activity in Normal Human Kidney Tubular HK-2 Cells via FOXO3/SOD2 Pathway. Journal of Food and Nutrition Research. 2018; 6(9):557-560. doi: 10.12691/jfnr-6-9-3


The anti-oxidant activity of daidzein was accessed by checking the expression level of superoxide dismutase 2 (SOD2) in HK-2 cell, a kidney proximal tubular cell line. SOD2 expression was increased in HK-2 cells treated by daidzein with a dose and time-dependent manner. Daidzein reduced the reactive oxygen species (ROS) level (about 60% compared to DMSO control with p = 0.005) in HK-2 cells. Daidzein caused FOXO3 to be translocated from cytosol into nucleus. Down-regulation of FOXO3 by transfection of siRNA against FOXO3 attenuated the expression of SOD2 and ROS level (p = 0.006) by daidzein. Taken together, this study has shown that daidzein displays anti-oxidant activity in HK-2 kidney proximal tubular cell line through up-regulation of FOXO3/SOD2 signaling pathway.

daidzein anti-oxidant HK-2 FOXO3 SOD2

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


Figure of 2


[1]  Sosa V, Moliné T, Somoza R, Paciucci R, Kondoh H, LLeonart ME. 2013. Oxidative stress and cancer: an overview. Ageing Res Rev 12: 376-390.
[2]  Dongyan T, Yinmao D, Li L, Yueheng L, Congfen H, Jixiang L. 2014. Antioxidant activity in mung bean sprouts and safety of extracts for cosmetic use. J Cosmet Sci 65: 207-216.
[3]  Teixeira CP, Simões RS, Santos MA, Calió ML, Soares JM Jr, Simões MJ, Bertoncini CR, Higa EM, Carbonel AF. 2014. Soybean concentrated extract counteracts oxidative stress in the uterus of rats. Climacteric 17: 402-409.
[4]  Georgetti SR, Casagrande R, Verri WA Jr, Lopez RF, Fonseca MJ. 2008. Evaluation of in vivo efficacy of topical formulations containing soybean extract. Int J Pharm 352: 189-196.
[5]  Aras AB, Guven M, Akman T, Ozkan A, Sen HM, Duz U, Kalkan Y, Silan C, Cosar M. 2015. Neuroprotective effects of daidzein on focal cerebral ischemia injury in rats. Neural Regen Res 10: 146-152.
[6]  Zaheer K, Akhtar MH. 2015. An Updated Review of Dietary Isoflavones: Nutrition, Processing, Bioavailability and Impacts on Human Health. Crit Rev Food Sci Nutr 57: 1280-1293.
[7]  D'Adamo CR, Sahin A. 2014. Soy foods and supplementation: a review of commonly perceived health benefits and risks. Altern Ther Health Med Winter; 20 Suppl. 1: 39-51.
[8]  Rüfer CE, Kulling SE, 2006. Antioxidant activity of isoflavones and their major metabolites using different in vitro assays. J Agric Food Chem 54: 2926-2931.
[9]  KrisEtherton PM, Keen CL. 2002. Evidence that the antioxidant flavonoids in tea and cocoa are beneficial for cardiovascular health. Curr Opin Lipidol 13: 41-49.
[10]  Flynn JM, Melov S. 2013. SOD2 in mitochondrial dysfunction and neurodegeneration. Free Radic Biol Med 62: 4-12.
[11]  Agharazii M, St-Louis R, Gautier-Bastien A, Ung RV, Mokas S, Larivière R, Richard DE. 2015. Inflammatory cytokines and reactive oxygen species as mediators of chronic kidney disease-related vascular calcification. Am J Hypertens 28: 746-755.
[12]  Kops GJ, Dansen TB, Polderman PE, Saarloos I, Wirtz KW, Coffer PJ, Huang TT, Bos JL, Medema RH, Burgering BM. 2002. Forkhead transcription factor FOXO3a protects quiescent cells from oxidative stress. Nature 419: 316-321.