Assessment of the Biological Activity in Human Milk Power Treated with Different Processes for Their Conservation

Ariana Rodríguez Arreola¹, Monique Lacroix², Josué Raymundo Solís Pacheco¹, Elisa García Morales³, José Alfonso Gutiérrez Padilla³, Eusebio Angulo Castellanos³, Juan Arturo Ragazzo Sánchez⁴ and Blanca Rosa Aguilar Uscanga^1,

¹Laboratorio de Microbiología Industrial. Centro Universitario de Ciencias Exactas e Ingenierías. Universidad de Guadalajara. 142, Boulevard General Marcelino García Barragán, Col. Olímpica, C.P. 44430. Guadalajara, Jalisco, México

²INRS-Institut Armand-Frappier, Research Laboratories in Sciences Applied to Food, Canadian Irradiation Centre, The Institute of Nutraceutical and Functional Foods (INAF), 531, Boulevard des Prairies, Laval, QC, Canada H7V 1B7

³Centro Universitario de Ciencias de la Salud. Universidad de Guadalajara. Hospital Civil de Guadalajara Fray Antonio Alcalde. Sierra Mojada 950, Independencia Oriente, 44340. Guadalajara Jalisco, México

⁴Tecnológico Nacional de México. Instituto Tecnológico de Tepic. Laboratorio Integral de Investigación en Alimentos Av. Tecnológico # 2595, Col. Lagos del Country. C.P. 63175. Tepic Nayarit México

Cite this paper:
Ariana Rodríguez Arreola, Monique Lacroix, Josué Raymundo Solís Pacheco, Elisa García Morales, José Alfonso Gutiérrez Padilla, Eusebio Angulo Castellanos, Juan Arturo Ragazzo Sánchez and Blanca Rosa Aguilar Uscanga. Assessment of the Biological Activity in Human Milk Power Treated with Different Processes for Their Conservation. Journal of Food and Nutrition Research. 2018; 6(5):329-334. doi: 10.12691/jfnr-6-5-8

Abstract

Human milk is the best food for newborns and infants for its combination of nutrients and biological complexity that allows the healthy grows and development of the infant. These components could be affected by thermic treatments of conservation as heat pasteurization, used in Human Milk Banks. The aim of this study was to evaluate the effects of different treatments applied to human milk in its biological activity (antiradical scavenging and anticancer properties). Human milk was heat pasteurized, also submitted high pressure and after it was spray-dried. Moreover, of UV-C or γ- irradiation was applied on spray dried human milk. HT-29 cell line was used for testing antiproliferative assay. The results of this essay showed that 1.25 mg/mL of human milk treated with high pressures and spray dried can attain CI₅₀. Human milk powder treated with γ-irradiation showed the highest inhibition activity of HT-29 cell line, and the pasteurized and spray dried human milk presents the lowest inhibition activity (p<0.05). The highest percentage of scavenging activity against the superoxide anion was in spray-drying treated with γ-irradiation, showing significantly higher activity against the O₂ anion as compared to all other treatments tested (p<0.05), followed by human milk powder treated by UV-C irradiation, high pressure and heat pasteurization. Human milk powder treated by High pressure showed higher antioxidant activity, however, the value was not significantly higher as compared to the other pasteurization treatments evaluated (p > 0.05). Spray-dried human milk treated with high pressure, UV-C and γ- irradiation conserved its biological activity, thus it’s could be possible to apply this technology in Human Milk Banks for conserving it.

Keywords:
human milk high pressures UV irradiation antiproliferative antioxidant

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