Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Journal of Food and Nutrition Research. 2018, 6(2), 96-102
DOI: 10.12691/jfnr-6-2-5
Open AccessArticle

Prevalence and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry

Zohreh Mashak1,

1Department of Food Hygiene, Karaj Branch, Islamic Azad University, Karaj, Iran

Pub. Date: February 10, 2018

Cite this paper:
Zohreh Mashak. Prevalence and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry. Journal of Food and Nutrition Research. 2018; 6(2):96-102. doi: 10.12691/jfnr-6-2-5


Escherichia coli O157:H7 is one of the most dangerous zoonotic pathogens of meat. The present investigation was done to study the prevalence and antibiotic resistance of E. coli O157:H7 strains isolated from raw meat samples. A total of 458 meat samples were collected. Samples were cultured using what? After which sorbitol negative isolates were analyzed for rfbO157 and flicH7 genes. Thirty-six out of 458 meat samples were positive for sorbitol negative strains of E. coli (7.86%). All of the sorbitol negative strains were also positive for rfbO157 and flicH7 genes. Prevalence of E. coli O157:H7 strains were 7.86%. The prevalence of E. coli O157:H7 in chicken meat was higher than the other samples (16.25%). The genes that encode resistance to ampicillin (CITM) (100%), gentamicin (aac(3)-IV) (94.44%) and tetracycline (tetA) (61.11%) had the highest prevalence. Escherichia coli O157:H7 strains from raw meat samples from ruminants and poultry had the highest resistance to ampicillin (100%), tetracycline (83.33%) and gentamicin (83.33%) respectively. Strains of antibiotic resistant E. coli 0157:H7 found in this present study are of public health importance.

Escherichia coli O157:H7 raw meat antibiotic resistance pattern antibiotic resistance genes

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Gould, L.H., Walsh, K.A., Vieira, A.R., Herman, K., Williams, I.T., Hall, A.J. and Cole, D, ''Surveillance for foodborne disease outbreaks-United States, 1998-2008'', MMWR Surveill Summ, 62. 1-34. 2013.
[2]  Havelaar, A.H., Kirk, M.D., Torgerson, P.R., Gibb, H.J., Hald, T., Lake, R.J., Praet, N., Bellinger, D.C., De Silva, N.R. and Gargouri, N, “World Health Organization Global estimates and regional comparisons of the burden of foodborne disease in 2010”, PLoS Med, 12. e1001923. 2015.
[3]  Control CfD and Prevention, “Surveillance for Foodborne Disease Outbreaks, United States, 2013, Annual Report”. Atlanta: US Department of Health and Human Services, 2014.
[4]  Scallan, E., Hoekstra, R.M., Angulo, F.J., Tauxe, R.V., Widdowson, M.A., Roy, S.L., Jones, J.L. and Griffin, P.M, “Foodborne illness acquired in the United States-major pathogens”, Emerg Infect Dis, 17, 2011.
[5]  Stewardson, A.J., Renzi, G., Maury, N., Vaudaux, C., Brassier, C., Fritsch, E., Pittet, D., Heck, M., van der Zwaluw, K. and Reuland, E.A, “Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Hospital Food: A Risk Assessment”, Infection Control & Hospital Epidemiology, 35. 375-383. 2014.
[6]  Luo, Y., Cui, S., Li, J., Yang, J., Lin, L., Hu, C., Jin, S., Ye, L., Zhao, Q. and Ma, Y, “Characterization of Escherichia coli isolates from healthy food handlers in hospital”, Microbial Drug Resistance, 17. 443-448. 2011.
[7]  Dao, H.T.A. and Yen, P.T, “Study of Salmonella, Campylobacter, and Escherichia coli contamination in raw food available in factories, schools, and hospital canteens in Hanoi, Vietnam”, Annals of the New York Academy of Sciences, 1081. 262-265. 2006.
[8]  Cooke, E.M., Kumar, P., Shooter, R., Rousseau, S. and Foulkes, A, “Hospital food as a possible source of Escherichia coli in patients”, The Lancet, 295. 436-437. 1970.
[9]  Momtaz, H., Farzan, R., Rahimi, E., Safarpoor Dehkordi, F. and Souod, N, “Molecular characterization of Shiga toxin-producing Escherichia coli isolated from ruminant and donkey raw milk samples and traditional dairy products in Iran”, The Scientific World Journal, 2012. 1342. 2012.
[10]  Momtaz, H., Dehkordi, F.S., Rahimi, E., Ezadi, H. and Arab, R, “Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant's meat”, Meat science, 95. 381-388. 2013.
[11]  Dehkordi, F.S., Yazdani, F., Mozafari, J. and Valizadeh, Y, “Virulence factors, serogroups and antimicrobial resistance properties of Escherichia coli strains in fermented dairy products”, BMC research notes, 7. 1. 2014.
[12]  Bai, X., Zhang, W., Tang, X., Xin, Y., Xu, Y., Sun, H., Luo, X., Pu, J., Xu, J. and Xiong, Y, “Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China”, Frontiers in microbiology, 7. 2016.
[13]  Dhaka, P., Vijay, D., Vergis, J., Negi, M., Kumar, M., Mohan, V., Doijad, S., Poharkar, K.V., Malik, S.S. and Barbuddhe, SB, “Genetic diversity and antibiogram profile of diarrhoeagenic Escherichia coli pathotypes isolated from human, animal, food samples and associated environmental sources”, Infection ecology & epidemiology, 6. 2016.
[14]  Wang, J., Stanford, K., McAllister, T.A., Johnson, R.P., Chen, J., Hou, H., Zhang, G. and Niu, Y.D, “Biofilm Formation, Virulence Gene Profiles, and Antimicrobial Resistance of Nine Serogroups of Non-O157 Shiga Toxin-Producing Escherichia coli”, Foodborne pathogens and disease, 2016.
[15]  Farshad, S., Ranijbar, R., Japoni, A., Hosseini, M., Anvarinejad, M. and Mohammadzadegan, R, “Microbial susceptibility, virulence factors, and plasmid profiles of uropathogenic Escherichia coli strains isolated from children in Jahrom, Iran”, Archives of Iranian Medicine, 15. 2012.
[16]  Amézquita-López, B.A., Quiñones, B., Soto-Beltrán, M., Lee, B.G., Yambao, J.C., Lugo-Melchor, O.Y. and Chaidez, C, “Antimicrobial resistance profiles of Shiga toxin-producing Escherichia coli O157 and Non-O157 recovered from domestic farm animals in rural communities in Northwestern Mexico”, Antimicrobial resistance and infection control, 5. 1. 2016.
[17]  Abdi, S., Ranjbar, R., Vala, M.H., Jonaidi, N., Bejestany, O.B. and Bejestany, F.B, “Frequency of bla TEM, bla SHV, bla CTX-M, and qnrA Among Escherichia coli Isolated From Urinary Tract Infection”, Archives of Clinical Infectious Diseases, 9. 2014.
[18]  Momtaz, H., Karimian, A., Madani, M., Dehkordi, F.S., Ranjbar, R., Sarshar, M. and Souod, N, “Uropathogenic Escherichia coli in Iran: serogroup distributions, virulence factors and antimicrobial resistance properties”, Annals of clinical microbiology and antimicrobials, 12. 1. 2013.
[19]  Sambrook, J. and Russell, D.W, “Molecular cloning: a laboratory manual. third”, Cold pring Harbor Laboratory Press, New York, 2001.
[20]  Desmarchelier, P.M., Bilge, S.S., Fegan, N., Mills, L., Vary, J.C. and Tarr, P.I, “A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide”, Journal of Clinical Microbiology, 36. 1801-1804. 1998.
[21]  Philpott, D. and Ebel, F, “E. coli: shiga toxin methods and protocols”, Springer Science & Business Media, 2003.
[22]  Momtaz, H., Dehkordi, F.S., Hosseini, M.J., Sarshar, M. and Heidari, M, “Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran”, Gut pathogens, 5. 1. 2013.
[23]  Wayne, P, “Clinical and Laboratory Standards Institute (CLSI) performance standards for antimicrobial disk diffusion susceptibility tests 19th ed”, approved standard, CLSI document M100-S19, 29. 2009.
[24]  Mead, P.S., Slutsker, L., Dietz, V., McCaig, L.F., Bresee, J.S., Shapiro, C., Griffin, P.M. and Tauxe, R.V, “Food-related illness and death in the United States”, Emerging infectious diseases, 5. 607. 1999.
[25]  Zarei, M., Basiri, N., Jamnejad, A. and Eskandari, M.H, “Prevalence of Escherichia coli O157: H7, Listeria monocytogenes and Salmonella spp. in beef, buffalo and lamb using multiplex PCR”, Jundishapur Journal of Microbiology, 6. 2013.
[26]  Hessain, A.M., Al-Arfaj, A.A., Zakri, A.M., El-Jakee, J.K., Al-Zogibi, O.G., Hemeg, H.A. and Ibrahim, I.M, “Molecular characterization of Escherichia coli O157: H7 recovered from meat and meat products relevant to human health in Riyadh, Saudi Arabia”, Saudi journal of biological sciences, 22. 725-729. 2015.
[27]  Zhang S, Zhu X, Wu Q, Zhang J, Xu X and Li H. Prevalence and characterization of Escherichia coli O157 and O157: H7 in retail fresh raw meat in South China. Annals of microbiology 2015; 65: 1993-1999.
[28]  Bekele, T., Zewde, G., Tefera, G., Feleke, A. and Zerom, K, “Escherichia coli O157: H7 in raw meat in Addis Ababa, Ethiopia: prevalence at an abattoir and retailers and antimicrobial susceptibility”, International Journal of Food Contamination, 1. 4. 2014.
[29]  Goncuoglu, M., Ormanci, F.S.B., Ayaz, N.D. and Erol, I, “Antibiotic resistance of Escherichia coli O157: H7 isolated from cattle and sheep”, Annals of microbiology, 60. 489-494. 2010.
[30]  Iweriebor, B.C., Iwu, C.J., Obi, L.C., Nwodo, U.U. and Okoh, A,I, “Multiple antibiotic resistances among Shiga toxin producing Escherichia coli O157 in feces of dairy cattle farms in Eastern Cape of South Africa”, BMC microbiology, 15. 1. 2015.
[31]  Kang, E., Hwang, S.Y., Kwon, K.H., Kim, K.Y., Kim, J.H. and Park, Y.H, “Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) from cattle in Korea between 2010 and 2011”, Journal of veterinary science, 15. 369-379. 2014.
[32]  Castillo, F.Y.R., González, F.J.A., Garneau, P., Díaz, F.M., Barrera, A.L.G. and Harel, J, “Presence of multi-drug resistant pathogenic Escherichia coli in the San Pedro River located in the State of Aguascalientes, Mexico”, Frontiers in microbiology, 4. 2013.
[33]  Tadesse, D.A., Zhao, S., Tong, E., Ayers, S., Singh, A., Bartholomew, M.J. and McDermott, P.F, “Antimicrobial drug resistance in Escherichia coli from humans and food animals, United States, 1950-2002”, Emerging infectious diseases, 18. 741. 2012.
[34]  Schroeder, C.M., Zhao, C., DebRoy, C., Torcolini, J., Zhao, S., White, D.G., Wagner, D.D., McDermott, P.F., Walker, R.D. and Meng, J, “Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food”, Applied and Environmental Microbiology, 68. 576-581. 2002.
[35]  Braoudaki, M. and Hilton, A.C, “Low level of cross-resistance between triclosan and antibiotics in Escherichia coli K-12 and E. coli O55 compared to E. coli O157”, FEMS microbiology letters, 235. 305-309. 2004.
[36]  Hemmatinezhad, B., Khamesipour, F., Mohammadi, M., Safarpoor Dehkordi, F. and Mashak, Z, “Microbiological Investigation of O-Serogroups, Virulence Factors and Antimicrobial Resistance Properties of Shiga Toxin‐Producing Escherichia Coli Isolated from Ostrich, Turkey and Quail Meats”, Journal of Food Safety, 35. 491-500. 2015.
[37]  Momtaz, H., Safarpoor Dehkordi, F., Taktaz, T., Rezvani, A. and Yarali, S, “Shiga toxin-producing Escherichia coli isolated from bovine mastitic milk: serogroups, virulence factors, and antibiotic resistance properties”, The Scientific World Journal, 2012. 1-9. 2012.