Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Journal of Food and Nutrition Research. 2018, 6(2), 69-73
DOI: 10.12691/jfnr-6-2-1
Open AccessArticle

Screening Study on Enzyme Inhibitory Activities of Some Mongolian Plant Leaves

Enkhtsetseg Enkhtuya1, 2, , Tomoko Shimamura2, , Takehiro Kashiwagi2 and Hiroyuki Ukeda3

1School of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar, Mongolia

2Faculty of Agriculture and Marine Science, Kochi University, Kochi, Japan

3Center for Regional Collaboration, Kochi University, Kochi, Japan

Pub. Date: February 01, 2018

Cite this paper:
Enkhtsetseg Enkhtuya, Tomoko Shimamura, Takehiro Kashiwagi and Hiroyuki Ukeda. Screening Study on Enzyme Inhibitory Activities of Some Mongolian Plant Leaves. Journal of Food and Nutrition Research. 2018; 6(2):69-73. doi: 10.12691/jfnr-6-2-1


In this study, 50% ethanol extract prepared from air-dried leaves of nine plants grown wildly in Mongolia were characterized for their inhibitory activity of tyrosinase, α-glucosidase, hyaluronidase, lipase, and angiotensin I-converting enzyme (ACE) in order to identify potential new sources for dietary prevention of certain non-communicable diseases and skin health promotion from domestic plant kingdom. The results of this study indicate that out of the selected nine Mongolian plant leaves, V.vitis-idaeae, R.altissimum, and R.acicularis leaves presented strong inhibitory activity of mushroom tyrosinase, R.acicularis and P.anomala leaves exhibited good anti α-glucosidase and anti hyaluronidase activity, V.vitis-idaeae leaf showed appreciable inhibition of lipase activity, and P.anomala leaf effectively inhibited ACE. Among them P.anomala and R.acicularis leaves were found to be the most promising natural source with multiple biological properties.

tyrosinase hyaluronidase α-glucosidase lipase angiotensin I-converting enzyme inhibitory activity plant leaves

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Enkhtuya E, Kashiwagi T, Shimamura T, Ukeda H, Tseye-Oidov O, "Screening study on antioxidant activity of plants grown wildly in Mongolia", Food Science and Technology Research, 20 (4). 891-897. 2014.
[2]  Enkhtuya E, Kashiwagi T, Shimamura T, Ukeda H, "Antioxidative constituents in the leaves of Paeonia anomala grown in Mongolia", Food Science and Technology Research, 23 (1). 63-70. 2017.
[3]  Cook N.C, Samman S, "Flavonoids-Chemistry, metabolism, cardioprotective effects, and dietary sources", Journal of Nutritional Biochemistry, 7. 66-76. 1996.
[4]  Kubo I, Kinst-Hori I, "Tyrosinase Inhibitors from Anise Oil", Journal of Agricultural and Food Chemistry, 46. 1268-1271. 1998.
[5]  Kakegawa H, Matsumoto H, Satoh T, "Activation of hyaluronidase by metallic salts and compound 48/80, and inhibitory effect of anti-allergic agents on hyaluronidase", Chemical and Pharmaceutical Bulletin, 33. 642-646. 1985.
[6]  Maeda Y, Yamamoto M, Matsui T, Sugiyama K, Yokota M, Nakagomi K, Tanaka H, Takahashi I, Kobayashi T, Kobayashi E, "Inhibitory effect of tea extracts on hyaluronidase (Studies on anti-allergic activity in tea. II)", Journal of the Food Hygienic Society of Japan, 31. 233-237. 1990.
[8]  Asha Gangadharan, Elizabeth Jacob and Densely Jose, "Phytochemical analysis, antibacterial and anti-hyaluronidase avtivity of three indigenous medicinal plants", World Journal of Pharmacy and Pharmaceutical Sciences, 3 (6). 751-761. 2014.
[9]  S.Momtaz, N.Lall and A.Basson. "Inhibitory activities of mushroom tyrosine and DOPA oxidation by plant extracts", South African Journal of Botany, 74. 577-582. 2008.
[10]  K.T.Lee, B.J.Kim, J.H.Kim, M.Y.Heo and H.P.Kim. "Biological screening of 100 plant extracts for cosmetic use (I): inhibitory acivities of tyrosinase and DOPA auto-oxidation", International Journal of Cosmetic Science, 19. 291-298. 1997
[11]  Anand Wardhan, Shabina Khan and Bhawana Pandey. "Screening of plant parts for anti-tyrosinase activity by tyrosinase assay using mushroom tyrosinase", Indian Journal of Scientific Research, 4 (1). 134-139. 2014.