Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2013, 1(6), 121-127
DOI: 10.12691/jfnr-1-6-2
Open AccessArticle

Effect of Food Preparation Technique on Antioxidant Activity and Plant Pigment Content in Some Vegetables Species

Violeta Mitic1, , Vesna Stankov Jovanovic1, Marija Dimitrijevic1, Jelena Cvetkovic1 and Gordana Stojanovic1

1Department of Chemistry, Faculty of Science and Mathematics, University of Nis Nis, Serbia

Pub. Date: November 12, 2013

Cite this paper:
Violeta Mitic, Vesna Stankov Jovanovic, Marija Dimitrijevic, Jelena Cvetkovic and Gordana Stojanovic. Effect of Food Preparation Technique on Antioxidant Activity and Plant Pigment Content in Some Vegetables Species. Journal of Food and Nutrition Research. 2013; 1(6):121-127. doi: 10.12691/jfnr-1-6-2

Abstract

Effect of food preparation technique on antioxidant activity and plant pigment content in broccoli, Brussels sprouts, white cabbage, kale, chard, spinach and garden patience were studied. The highest content of chlorophyll a was detected in garden patience (0.837 mg/g f.v.) and the lowest was in brussels sprout (0.047 mg/g f.v.), while chlorophyll b and carotenoid content was lower. Boiling had influence on pigment content in selected vegetables. Different food preparation technique showed influence on antioxidant activity of vegetables extracts. Boiled vegetables extracts showed highest antioxidant activity, which indicates that valuable phytochemicals remains in water during cooking process. Highest reducing power is detected for frozen garden patience extract (16.775 AAE/10 g f.v.), while DPPH, ABTS and FRAP assays showed highest antioxidant activities for boiled and microwave cooked vegetables. Correlations among antioxidant activities based on ABTS, DPPH, FRAP and TRP assays were positively high and r ranged between 0.68 and 0.99.

Keywords:
antioxidant activity leafy green vegetables chlorophyll carotenoids correlation

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References:

[1]  Faller. A. L. K. and Fialho. E. “The antioxidant capacity and polyphenol content of organic and conventional retail vegetables after domestic cooking,” Food Research International. 42(1). 210-215. Oct.2009.
 
[2]  Gupta. S., Jyothi Lakshmi. A., Manjunath. M. N. and Prakash. J. “Analysis of nutrient and antinutrient content of underutilized green leafy vegetables, “LWT- Food Science and Technology. 38(6). 339-345. Jun.2005.
 
[3]  Alia. M., Horcajo. C., Bravo. L., and Goya. L. “Effect of grape antioxidant dietary fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats,” Nutrition Research. 23(9). 1251-1267. Sep.2003.
 
[4]  Velioglu.Y. S., Mazza. G., Gao. L. and Oomah. B. D. “Antioxidant activity and total phenolics in selected fruits. vegetables. and grain products,” Journal of Agricultural Food & Chemistry. 46. 4113-4117. Aug.1998.
 
[5]  Emmons. C. L., Peterson. D. M. and Paul. G. L.” Antioxidant capacity of oat (Avena sativa L.) extracts. 2. In vitro antioxidant activity and contents of phenolic and tocopherol antioxidants,” Journal of Agricultural Food & Chemistry. 47(12). 4894-4898. Dec.1999.
 
[6]  Re. R., N. Pellegrini., A. Proreggente., A. Pannala., M. Yang and C. Rice-Evans. “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free Radical and Biological Medicine. 26(9-10). 1231-1237. May.1999.
 
[7]  Zhang. D., and Hamauzu. Y. “Phenolics. ascorbic acid. carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking,” Food Chemistry. 88(4). 503-509. Dec.2004.
 
[8]  Ismail. A., Marjan. Z. M. and Foong. C. W. “Total antioxidant activity and phenolic content in selected vegetables,” Food Chemistry. 87(4). 581-586. Oct.2004.
 
[9]  Turkmen, N., Sari, F. and Velioglu, S. “The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables,” Food Chemistry 93: 713-718. Dec. 2005.
 
[10]  Oyaizu M. “Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine,” Japanese Journal of Nutrition 44. 307-315. 1986
 
[11]  T. Hatano., H. Kagawa., T. Yasuhara. and T. Okuda. “Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects,” Chemical and Pharmaceutical Bulletin 36(6). 2090-2097. Jun.1988.
 
[12]  Wong S.P., Lai P.L., Jen H.W.K. “Antioxidant activities of aqueous extracts of selected plants. “Food Chemistry. 99. 775-783. Aug. 2006.
 
[13]  Kidmose. U., Edelenbos. M., Christiansen. L. P. and Hegelund. E. “Chromatographic determination of changes in pigments in spinach (Spinacia Oleracea L.) during processing,” Journal of Chromatographic Science. 43(9). 466-472. Oct.2005.
 
[14]  Lopez-Ayerra. B., Murcia. M. A. and Garcia-Carmona. F. “Lipid peroxidation and chlorophyll levels in spinach during refrigerated storage and after industrial processing,” Food Chemistry. 61. 113–118. Jan.1998.
 
[15]  Katalinić. V., Miloš. M., Kulisić. T. and Jukić.M. “Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols,“ Food Chemistry. 94 (2006) 550-557. Dec.2004.
 
[16]  Jayanthi. P. and P. Lalitha. “Reducing power of the solvent extracts of eichhornia crassipes (mart.) Solms,” International Journal of Pharmacy and Pharmaceutical Sciences. 3. 126-128. Mar.2011.
 
[17]  Sulaiman. S.F., Sajak. A.A.B., Ooi. K.L. and Supriatno. Seow. E.M. “Effect of solvents in extracting polyphenols and antioxidants of selected raw vegetables,” Journal of Food Composition and Analysis. 24(4-5) 506-515. Jun-Aug.2011.
 
[18]  Benzie. F.F. and Strain. J.J. Methods in enzymology, Lester Packer of Publishers, (1999) Ferric Reducing/ Antioxidant Power Assay: Direct Measure of Total antioxidant Activity of Biological Fluids and Modified Version for Simultaneous Measurement of Total Antioxidant Power and Ascorbic Acid Concentration.vol. 299:15-23.
 
[19]  Thaiponga. K., Boonprakoba. U., Crosbyb. K., Cisneros-Zevallos.L. and Hawkins Byrne. D. “Comparison of ABTS. DPPH. FRAP and ORAC assays for estimating antioxidant activity from guava fruit extracts, Journal of Food Composition and Analysis. 19. 669-675. Jan. 2006.
 
[20]  Tong Szeto. Y., Tomlinson. B. and Benzie. I. F. F. “Total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications for dietary planning and food preservation,” British Journal of Nutrition. 87(1). 55-59. Jan.2002.
 
[21]  M. B. Arnao. “Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case,” Trends in Food Science and Technology. 11. 419-421(3). Nov. 2000.
 
[22]  A. Wojdylo., J. Oszmianski. and R. Czemerys. “Antioxidant activity and phenolic compounds in 32 selected herbs,” Food Chemistry. 105. 940-949. Apr. 2007.
 
[23]  R. L. Prior., X. Wu. and K. Schaich. “Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements,” Journal of Agricultural and Food Chemistry. 53: 4290-4302. Apr. 2005.