Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2017, 5(12), 914-924
DOI: 10.12691/jfnr-5-12-6
Open AccessArticle

Amaranth Protein Improves Lipid Profile and Insulin Resistance in a Diet-induced Obese Mice Model

Abraham Escobedo-Moratilla1, Aida J. Velarde-Salcedo1, Cynthia V. Magaña-Hernández1, Alberto Barrera-Pacheco1, Eduardo Espitia-Rangel2, Alfredo Herrera-Estrella3 and Ana P. Barba de la Rosa1,

1IPICYT, Instituto Potosino de Investigación Científica y Tecnológica A.C. Camino a la Presa San José 2055, Col. Lomas 4ª Sección, C.P. 78216, San Luis Potosí, Mexico

2INIFAP, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Campo Experimental Valle de México, Km 13.5 Carr Los Reyes-Texcoco,C.P. 56250 Texcoco Estado de México

3Laboratorio Nacional de Genómica para la Biodiversidad, CINVESTAV-Irapuato, Irapuato, 36821, México

Pub. Date: December 02, 2017

Cite this paper:
Abraham Escobedo-Moratilla, Aida J. Velarde-Salcedo, Cynthia V. Magaña-Hernández, Alberto Barrera-Pacheco, Eduardo Espitia-Rangel, Alfredo Herrera-Estrella and Ana P. Barba de la Rosa. Amaranth Protein Improves Lipid Profile and Insulin Resistance in a Diet-induced Obese Mice Model. Journal of Food and Nutrition Research. 2017; 5(12):914-924. doi: 10.12691/jfnr-5-12-6

Abstract

Amaranth has been claimed as functional food, but its function on obesity-related disorder is not fully known. The aim of this study was to analyse the effect of amaranth protein intake on blood lipids profile and insulin resistance in diet-induced obese mice. The effect of soybean protein was also analysed for comparative purposes. C57BL-6 mice were fed for eight weeks with regular or high fat diet. Amaranth or soybean protein isolates (10 mg/kg) were supplied via oral administration. Changes in body weight, adipose tissue, total cholesterol, triglycerides, insulin, a glucose tolerance test, as well as the expression of lipid metabolism-related genes were measured. Our results have shown that amaranth protein induces a decrease in plasma insulin in mice fed with a regular diet, whereas a decrease in triglycerides was observed in mice fed with high fat diet. Furthermore, down-regulation of Tnf-α and Res, suggested the inhibition of inflammation state. The present study demonstrates that amaranth protein, but not soybean protein, improves the obese mice health, and the hormonal modulation (Lep, Fasn, Lpl) could lead to new mechanism of action by which amaranth consumption exerts its beneficial health effect.

Keywords:
amaranth cytokines insulin resistance obesity protein isolate soybean

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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References:

[1]  WHO. World Health Organization. Obesity and Overweight. http://www.who.int/mediacentre/factsheets/fs311/en/. [Accessed May. 31, 2017).
 
[2]  Majumder, K., Mine, Y. and Wu, J.P, “The potential of food protein-derived anti-inflammatory peptides against various chronic inflammatory disease,” Journal of the Science of Food and Agriculture, 9(7)6. 2303-2311. May. 2016.
 
[3]  Siro, I., Kapolna, E., Kapolna, B. and Lugasi, A, “Functional food. Product development, marketing and consumer acceptance--a review,” Appetite, 51(3). 456-467. Nov. 2008.
 
[4]  Wanezaki, S., Tachibana, N., Nagata, M., Saito, S., Nagao, K., Yanagita, T. and Kohno, M, “Soy β-conglycinin improves obesity-induced metabolic abnormalities in a rat model of nonalcoholic fatty liver disease,” Obesity Research & Clinical Practice, 9(2). 168-174. Apr. 2015.
 
[5]  Haliga, R., Mocanu, V., Oboroceanu, T., Stitt, P.A. and Luca, V.C, “The effects of dietary flaxseed supplementation on lipid metabolism in streptozotocin-induced diabetic hamsters,” Revista Medico-Chirurgicala a Societatii de Medici Si Naturalisti Din Iasi, 111(2). 472-476. Apr/Jun. 2007.
 
[6]  Yeh, W.J., Yang, H.Y. and Chen, J.R, Soy β-conglycinin retards progression of diabetic nephropathy via modulating the insulin sensitivity and angiotensin-converting enzyme activity in rats fed with high salt diet,” Food and Function, 5(11). 2898-2904. Nov. 2014.
 
[7]  Robles-Ramirez, M.C., Ramon-Gallegos, E., Reyes-Duarte, F.J. and Mora-Escobedo, R, “Effect of germinated soy protein on the growth of HeLa cervical cancer cells in female athymic mice,” Nutrition Cancer, 64(8). 1261-1268. 2012.
 
[8]  Yang, H.Y., Tzeng, Y.H., Chai, C.Y., Hsieh, A.T., Chen, J.R., Chang, L.S. and Yang, S.S, “Soy protein retards the progression of non-alcoholic steatohepatitis via improvement of insulin resistance and steatosis,” Nutrition, 27(9). 943-948. Sep. 2011.
 
[9]  Huerta-Ocampo, J.A, and Barba de la Rosa, A.P, “Amaranth: A pseudo-cereal with nutraceutical properties,” Current Nutrition & Food Science, 7(1). Feb. 1-9, 2011.
 
[10]  Dia, V.P., Torres, S., De Lumen, B.O., Erdman, J.W. and González de Mejia, E, “Presence of lunasin in plasma of men after soy protein consumption,” Journal of Agricultural and Food Chemistry, 57(4). 1260-1266. Feb. 2009.
 
[11]  Velarde-Salcedo, A.J., Barrera-Pacheco, A., Lara-Gonzalez, S., Montero-Moran, G.M., Diaz-Gois, A., Gonzalez de Mejia, E. and Barba de la Rosa, A.P, “In vitro inhibition of dipeptidyl peptidase IV by peptides derived from the hydrolysis of amaranth (Amaranthus hypochondriacus L.) proteins,” Food Chemistry, 136(2). 758-764. Jan. 2013.
 
[12]  Barba de la Rosa, A.P., Montoya, A.B., Martinez-Cuevas, P., Hernandez-Ledesma, B., Leon-Galvan, M.F., De Leon-Rodriguez, A. and Gonzalez, C, “Tryptic amaranth glutelin digests induce endothelial nitric oxide production through inhibition of ACE: antihypertensive role of amaranth peptides,” Nitric Oxide, 23(2):106-111. Sep. 2010.
 
[13]  Kim, H.K., Kim, M.J., Cho, H.Y., Kim, E.K. and Shin, D.H, “Antioxidative and anti-diabetic effects of amaranth (Amaranthus esculantus) in streptozotocin-induced diabetic rats,” Cell Biochemistry and Function, 24(3). 195-199. May/Jun. 2006.
 
[14]  Kim, H.K., Kim, M.J. and Shin, D.H, “Improvement of lipid profile by amaranth (Amaranthus esculantus) supplementation in streptozotocin-induced diabetic rats,” Annals of Nutrition and Metabolism, 50(3). 277-281. Feb. 2006.
 
[15]  Areas, J.A. and Lawrie, R.A, “Effect of lipid-protein interactions on extrusion of offal protein isolates,” Meat Science, 11(4). 275-299. 1984.
 
[16]  Martinez, E.N. and Anon, M.C, “Composition and structural characterization of amaranth protein isolates. An electrophoretic and calorimetric study,” Journal of Agricultural and Food Chemistry, 44(9). 2523-2530. Sep. 1996.
 
[17]  AOAC, “Official Methods of Analysis,” Association of Official Analytical Chemists, Washington, D.C. 18th edn. 2007.
 
[18]  Andrikopoulos, S., Blair, A.R., Deluca, N., Fam, B.C. and Proietto, J, “Evaluating the glucose tolerance test in mice,” American Journal of Physiology- Endocrinology and Metabolism, 295(6). E1323-1332. Dec. 2008.
 
[19]  Utsumi, S, “Plant food protein engineering,” Advances in Food Nutrition and Research, 36. 89-208. 1992.
 
[20]  Barba de la Rosa, A.P., Paredes-López, O. and Gueguen, J, “Characterization of amaranth globulins by ultracentrifugation and chromatographic techniques,” Journal of Agricultural and Food Chemistry, 40. 937-40. 1992.
 
[21]  Yang, A., Yu, X., Zheng, A. and James, A.T, “Rebalance between 7S and 11S globulins in soybean seeds of differing protein content and 11SA4,” Food Chemistry, 210(1). 148-155. Nov. 2016.
 
[22]  Mendonça, S., Saldiva, P.H., Cruz, R.J. and Areas, J.A.G, “Amaranth protein presents cholesterol-lowering effect,” Food Chemistry, 116(3). 738-742. Oct. 2009.
 
[23]  Lado, M.B., Burini, J., Rinaldi, G., Anon, M.C. and Tironi, V.A, “Effects of the dietary addition of amaranth (Amaranthus mantegazzianus) protein isolate on antioxidant status, lipid profiles and blood pressure of rats,” Plant Foods for Human Nutrition, 70(4). 371-379. Dec. 2015.
 
[24]  Imai, S, “Soybean and processed soy foods ingredients, and their role in cardiometabolic risk prevention,” Recent Patents on Food, Nutrition & Agriculture, 7(2). 75-82. 2015.
 
[25]  Eller, L.K. and Reimer, R.A, “A high calcium, skim milk powder diet results in a lower fat mass in male, energy-restricted, obese rats more than a low calcium, casein, or soy protein diet,” Journal of Nutrition, 140(7). 1234-1241. Jul. 2010.
 
[26]  Yan, L., Graef, G.L., Nielsen, F.H., Johnson, L.K. and Cao, J, “Soy protein is beneficial but high-fat diet and voluntary running are detrimental to bone structure in mice,” Nutrition Research, 35(6). 523-31. Jun. 2015.
 
[27]  Hakkak, R., Zeng, H., Dhakal, I.B. and Korourian, S, “Short- and long-term soy diet versus casein protects liver steatosis independent of the arginine content,” Journal of Medicinal Food, 18(11). 1274-1280. Nov. 2015.
 
[28]  Velasquez, M.T. and Bhathena, S.J, “Role of dietary soy protein in obesity,” International Journal of Medical Science, 4(2). 72-82. 2007.
 
[29]  Clifton, P, “Effects of a high protein diet on body weight and comorbidities associated with obesity,” British Journal of Nutrition, 108(S2). S122-129. Aug. 2012.
 
[30]  Diaz-Rúa, R., Keijer, J., Palou, A., van Schothorst, E.M. and Oliver, P, “Long-term intake of a high-protein diet increases liver triacylglycerol deposition pathways and hepatic signs of injury in rats,” Journal of Nutrition Biochemistry, 46. 39-48. Aug. 2017.
 
[31]  Liisberg, U., Myrmel, L.S., Fjaere, E., Ronnevik, A.K., Bjelland, S., Fauske, K.R., Holm, J.B., Basse, A.L., Hansen, J.B., Liaset, B., Kristiansen, K. and Madsen, L, “The protein source determines the potential of high protein diets to attenuate obesity development in C57BL/6J mice,” Adipocyte, 5(2). 196-211. Mar. 2016.
 
[32]  Gorinstein, S., Delgado-Licon, E., Pawelzik, E., Permady, H.H., Weisz, M, and Trakhtenberg, S, “Characterization of soluble amaranth and soybean proteins based on fluorescence, hydrophobicity, electrophoresis, amino acid analysis, circular dichroism, and differential scanning calorimetry measurements,” Journal of Agricultural and Food Chemistry, 49(11). 5595-5601. Nov. 2001.
 
[33]  Chen, X, and Yang, W, “Branched-chain amino acids and the association with type 2 diabetes,” Journal of Diabetes Investigation, 6(4). 369-370. Jul. 2015.
 
[34]  Green, C.R., Wallace, M., Divakaruni, A.S., Phillips, S.A., Murphy, A.N., Ciaraldi, T.P, and Metallo, C.M, “Branched-chain amino acid catabolism fuels adipocyte differentiation and lipogenesis,” Nature Chemical Biology, 12(1). 15-21. Jan. 2016.
 
[35]  Gharibeh, M.Y., Al-Tawallbeh, G.M., Abboud, M.M., Radaideh, A., Alhader, A.A, and Khabour, O.F, “Correlation of plasma resistin with obesity and insulin resistance in type 2 diabetic patients,” Diabetes & Metabolism, 36(6 Pt1). 443-449. Dec. 2010.
 
[36]  Jiang, Y., Lu, L., Hu, Y., Li, Q., An, C., Yu, X., Shu, L., Chen, A., Niu, C., Zhou, L, and Yang, Z, “Resistin induces hypertension and insulin resistance in mice via a TLR4-dependent pathway,” Scientific Reports, 6. 22193. Feb. 2016.
 
[37]  Kadowaki, T., Yamauchi, T., Kubota, N., Hara, K., Ueki, K, and Tobe, K, “Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome,” Journal of Clinical Investigation, 116(7). 1784-1792. Jul. 2006.
 
[38]  Wang, H, and Eckel, R.H, “Lipoprotein lipase: from gene to obesity,” American Journal of Physiology- Endocrinology and Metabolism, 297(2). E271-288. Aug. 2009.
 
[39]  Havel, P.J, “Role of adipose tissue in body-weight regulation: mechanisms regulating leptin production and energy balance,” Proceedings of the Nutrition Society, 59(3). 359-371. Aug. 2000.
 
[40]  McManus, B.L., Korpela, R., Speakman, J.R., Cryan, J.F., Cotter, P.D, and Nilaweera, K.N, “Bovine serum albumin as the dominant form of dietary protein reduces subcutaneous fat mass, plasma leptin and plasma corticosterone in high fat-fed C57/BL6J mice,” British Journal of Nutrition, 114(4). 654-662. Aug. 2015.
 
[41]  Clarke, S.D, “Regulation of fatty acid synthase gene expression: an approach for reducing fat accumulation,” Journal of Animal Science, 71(7). 1957-1965. Jul. 1993.
 
[42]  Frayn, K.N., Fielding, B.A, and Karpe, F, “Adipose tissue fatty acid metabolism and cardiovascular disease,” Current Opinion in Lipidology, 16(4). 409-415. Aug. 2005.
 
[43]  Gan, L., Liu, Z., Cao, W., Zhang, Z, and Sun, C, “FABP4 reversed the regulation of leptin on mitochondrial fatty acid oxidation in mice adipocytes,” Scientific Reports, 5. 13588. Aug. 2015.