Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2017, 5(11), 867-873
DOI: 10.12691/jfnr-5-11-11
Open AccessArticle

Total and Thermo-Tolerant Coliforms, Salmonella spp., Escherichia coli, Pseudomonas spp and Listeria monocytogenes in Broilers Chicken Meat Processing Chain in Southern Brazil

Denise Oliveira Pacheco1, Jacqueline Valle de Bairros1, Luciana Diéguez Ferreira Passos1, Márcia Rúbia Duarte Buchweitz1, Kelly Lameiro Rodrigues1, Elizabete Helbig1 and Eliezer Avila Gandra2,

1Postgraduate Program in Nutrition and Food, School of Nutrition, Federal University of Pelotas, Pelotas, Brazil

2Center of Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, Pelotas, Brazil

Pub. Date: November 20, 2017

Cite this paper:
Denise Oliveira Pacheco, Jacqueline Valle de Bairros, Luciana Diéguez Ferreira Passos, Márcia Rúbia Duarte Buchweitz, Kelly Lameiro Rodrigues, Elizabete Helbig and Eliezer Avila Gandra. Total and Thermo-Tolerant Coliforms, Salmonella spp., Escherichia coli, Pseudomonas spp and Listeria monocytogenes in Broilers Chicken Meat Processing Chain in Southern Brazil. Journal of Food and Nutrition Research. 2017; 5(11):867-873. doi: 10.12691/jfnr-5-11-11

Abstract

Sixty-six samples were collected from a broiler chicken abattoir. Eighteen samples were collected from the retail market in Southern Brazil, where the slaughtered broilers were commercialized, so that the microbiological assessment of the samples in the broiler industrial chain could be undertaken. And eighteen samples submitted to clients´ common home procedures were similarly assessed. All samples were evaluated by quantifying total and thermotolerant coliforms, psycrotrophic bacteria, Pseudomonas spp, Escherichia coli and research for Salmonella spp and Listeria monocytogenes. Results on counts and on the occurrence of microorganisms in samples from specific sites in the broiler processing chain demonstrate the need for a re-assessment of hygiene practices throughout the whole process and educational activities should be direct to all people involved, including consumers.

Keywords:
pathogenic microorganisms chicken industrial hygienic Listeria monocytogenes

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References:

[1]  Carvalho, A. C. F. B., Cortez, A.L.L., Salotti, B.M., Bürger, K.P. and Vidal-Martins, A.M.C. “Presença de microrganismos mesófilos, psicrotróficos e coliformes em diferentes amostras de produtos avícolas” Arquivo Instituto Biologia, 72(3). 303-307. 2005.
 
[2]  Jay, J. M., Loessner, M. J., and Golden, D. A. Modern Food Microbiology (7th ed). New York, NY: Springer Science. 2005.
 
[3]  Caldera, L., Franzetti, L., Van Coillie, E., De Vos, P., Stragier, P., De Block, J., Heyndrickx, M “Identification, enzymatic spoilage characterization and proteolytic activity quantification of Pseudomonas spp. isolated from different foods” Food Microbiology, 54. 142-153. 2016.
 
[4]  Vaillant, V., De Valk, H., Baron, E., Ancelle, T., Colin, P., Delmas, M. C., Dufour, B., Pouillot, R., Le Strat, Y., Weinbreck, P., Jougla, E. and Desenclos, J. C, “Foodborne infections in France” Foodborne Pathogens and Disease, 2. 221-232. 2005.
 
[5]  Lambertz, S.T., Ivarsson, S., Lopes-Valladares, G., Sidstedt, M., Lindqvist, R, “Subtyping of Listeria monocytogenes isolates recovered from retail ready-to-eat foods, processing plants and listeriosis patients in Sweden 2010” International Journal of Food Microbiology, 166(1).186-192.2013.
 
[6]  López, V., Ortiz, S., Corujo, A., López, P., Poza, D., Navas, J., Moreno, R. and Martínez-Suárez, J. V, “Different Contamination Patterns of Lineage I and II Strains of Listeria monocytogenes in a Spanish Broiler Abattoir. Poultry Science, 87(9). 1874-82. 2008.
 
[7]  Goh, S. G., Kuan, C. H., Loo, Y. Y.,Chang, W. S., Lye, Y. L., Soopna, P., Tang, J. Y., Nakaguchi, Y., Nishibuchi, M., Afsah-Hejri, L. and Son, R. Listeria monocytogenes in retailed raw chicken meat in Malaysia, 91(10). 2686-2690. 2012.
 
[8]  Svobodová, I., Bořilová, G., Hulánková, R. and Steinhauserová, I, “Microbiological quality of broiler carcasses during slaughter processing” ActaVeterinaria Brno, 81. 37-42. 2012.
 
[9]  Galarz, L. A., Fonseca, G. G. and Pretince-Hernández, C. “Crescimento microbiano em produtos à base de peito de frango durante simulação da cadeia de abastecimento” Ciência e Tecnologia de Alimentos” 30(4). 870-877. 2010.
 
[10]  Downes, F. P. and Ito, H. Compendium of methods for the microbiological examination of foods. Washington: American Public Health Association (APHA). 2001.
 
[11]  International Organization for Standardization. Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: Detection method, International Standard ISO 11290-1. Geneva: International Organization for Standardization (ISO). 1996.
 
[12]  Brasil. “Regulamento Técnico sobre padrões microbiológicos para alimentos. Resolução RDC nº 12, January 02, 2012, 2001 [Online]. Available: http://portal.anvisa.gov.br/wps/wcm/connect/a47bab8047458b909541d53fbc4c6735/RDC_12_2001.pdf?MOD=AJPERES [Acessed Feb. 3, 2016].
 
[13]  Chambers, J. V. The microbiology of raw milk. In: R. K. ROBINSON (Eds), Dairy microbiology handbook: the microbiology of milk and milk products. (pp. 39-90). New York: John Wiley and Sons. 2002.
 
[14]  Andrew, D. and Ron, B. The microbiology of meat and poultry. London: Blackie Academic Professional. 1998.
 
[15]  Northcutt, J. K., Berrang, M. E., Dickens, J. A., Fletcher, D. L. and Cox, N. A, “Effect of broiler age, feed withdrawal, and transportation on levels of coliforms, Campylobacter, Escherichia coli and Salmonella on carcasses before and after immersion chilling” Poultry Science, 82(1).169-173. 2003.
 
[16]  Álvarez-Astorga, M., Capita, R., Alonso, C.C, Moreno, B. and Garcia, M.C.F, “Microbiological quality of retail chicken by-products in Spain” Meat Science, 62(1). 45-50. 2002.
 
[17]  Mehyar, G., Blank, G., Han, J. H., Hydamaka, A. and Holley, R. A, “Effectiveness of trisodium phosphate, lactic acid and commercial antimicrobials against pathogenic bacteria on chicken skin” Food Protection Trends, 25. 351-362. 2005.
 
[18]  Thomas, C. J. and Mcmeekin, T. A, “Effect of water immersion on the microtopography of the skin of chicken carcasses” Journal of the Science of Food and Agriculture, 33. (549-554). 1982.
 
[19]  Lillard, H. S, “The impact of commercial processing procedures on the bacterial contamination and cross contamination of broiler carcasses” Journal Food Protection, 53(3). 202-204. 1990.
 
[20]  International Commission on Microbiological Specifications for Foods. Microbial ecology of foods commodities. (2 ed). New York: Kluwer Academic, Plenum Publishers. 2005.
 
[21]  Barbalho, T. C. F., Almeida, P. F., Almeida, R. C. C. and Hofer, E,” Prevalence of Listeria spp. at a poultry processing plant in Brazil and a phage test for rapid confirmation of suspect colonies” Food Control, 16. 211-216. 2005.
 
[22]  Chiarini, E., Tyler, K., Farber, J. M., Pagotto, F. and Destro M, “Listeria monocytogenes in two different poultry facilities: Manual and automatic evisceration, Poultry Science, 88(4). 791-797. 2009.
 
[23]  Galvão, N. N., Chiarini, E., Destro, M.T., Ferreira, M.A. and Nero, A, “PFGE characterisation and adhesion ability of Listeria monocytogenes isolates obtained from bovine carcasses and beef processing facilities” Meat science, 92 (4). 635-43. 2012.
 
[24]  Miettinen, M. K., Palmu, L., Bjorkroth, K. J., and Korkeala, H, “Prevalence of Listeria monocytogenes in broilers at the abattoir, processing plant, retail level” Journal of Food Protection, 64. 994-999. 2001.
 
[25]  Kozačinski, L., Hadžiosmanović, M and Zdolec, N, “Microbiological quality of poultry meat on the Croatian market” Veterinarski Arhiv, 76. 305-313. 2006.
 
[26]  Yan, H., Neogi, S. B., Mo, Z.; Guan, W., Shen, Z., Zhang, S.; LI, L., Yamasaki, S., Shi, L. and Zhong, N, “Prevalence and characterization of antimicrobial resistance of foodborne Listeria monocytogenes isolates in Hebei province of Northern China, 2005-2007” International Journal of Food Microbiology, 144(2). 310-316. 2010.