Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Journal of Food and Nutrition Research. 2017, 5(9), 668-673
DOI: 10.12691/jfnr-5-9-6
Open AccessArticle

Anti-inflammatory Activities of Ethanolic Extracts of curcuma Longa (Turmeric) and cinnamon (Cinnamomum verum)

Khaled Qabaha1, Saleh Abu-Lafi2 and Fuad Al-Rimawi3,

1Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Arab American University, Jenin, Palestine

2Faculty of Pharmacy, Al-Quds University, Jerusalem, Palestine

3Chemistry Department, Faculty of Science and Technology, Al-Quds University, East Jerusalem, Palestine

Pub. Date: August 23, 2017

Cite this paper:
Khaled Qabaha, Saleh Abu-Lafi and Fuad Al-Rimawi. Anti-inflammatory Activities of Ethanolic Extracts of curcuma Longa (Turmeric) and cinnamon (Cinnamomum verum). Journal of Food and Nutrition Research. 2017; 5(9):668-673. doi: 10.12691/jfnr-5-9-6


Curcuma longa and Cinnamon are used in folkloric medicine and thought to have different pharmacological activities including anti-inflammatory effects. The objective of this work is to evaluate the in vitro inhibitory effect of Curcuma longa and Cinnamon ethanolic extracts on Lipopolysacaride (LPS)-induced Interlukin-6 (IL-6) and Tumor Necrosis Factor-α (TNF-α) by polymorphonuclear Cells (PMNCs). Polymorphonuclear cells were isolated from the whole blood using Histopaque (Ficol-1077) method and then cultured in an enriched Roswell Park Memorial Institute (RBMI) medium. The concentrations of TNF-α and IL-6 in the supernatant were measured after 24 h and compared using paired-samples t test. The Curcuma longa and Cinnamon extracts have shown significant reduction in the levels of both Il-6 and TNF-α. HPLC analysis of Curcuma longa extract revealed that it contains curcumin, demethoxycurcumin, and bisdemethoxycurcumin while the major compound in the extract of cinnamon was found to be cinnamic acid. Reduction in the levels of IL-6 and TNF-α upon effect of the plants” extract is an indication of their anti-inflammatory effects. The observed anti-inflammatory effect may be due to the presence of curcuminoids and cinnamic acid from Curcuma longa and Cinnamon, respectively.

anti-inflammatory Curcuma longa cinnamon TNF-alpha curcuminoids cinnamic acid

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Iwata, N., Kainuma, M., Kobayashi, D., Kubota, T., Sugawara, N., Uchida, A., Ozono, S., Yamamuro, Y., Furusyo, N., Ueda, K., Tahara, E., Shimazoe, T., 2016. “The Relation between Hepatotoxicity and the Total Coumarin Intake from Traditional Japanese Medicines Containing Cinnamon Bark.” Front. Pharmacol. 7: 174.
[2]  Bimonte, S., Barbieri, A., Leongito, M., Piccirillo, M., Giudice, A., Pivonello, C., de Angelis, C., Granata, C., Palaia, R., Izzo, F., 2016. “Curcumin AntiCancer Studies in Pancreatic Cancer.” Nutrients 8(7), 433.
[3]  Hugar, S. S., Patil, S., Metgud, R., Nanjwade, B., Hugar, S. M. 2016. Influence of application of chlorhexidine gel and curcumin gel as an adjunct to scaling and root planing: A interventional study. Journal of Natural Science, Biology, and Medicine, 7(2), 149-154.
[4]  Sarkar, A., De, R., Mukhopadhyay, A. K., 2016. Curcumin as a potential therapeutic candidate for Helicobacter pylori associated diseases. World Journal of Gastroenterology, 22(9), 2736-2748.
[5]  He, Y., Yue, Y., Zheng, X., Zhang, K., Chen, S., Du, Z. 2015. Curcumin, Inflammation, and Chronic Diseases: How Are They Linked? Molecules. 20(5): 9183-9213.
[6]  Akilen, R., Tsiami, A., Devendra, D., Robinson, N., 2012. Cinnamon in glycaemic control: systematic review and meta analysis. Clin Nutr. 31 (5): 609-615.
[7]  Zainol, N. A., Ming, T. S., Darwis, Y., 2015. Development and Characterization of Cinnamon Leaf Oil Nanocream for Topical Application. Indian Journal of Pharmaceutical Sciences, 77(4), 422-433.
[8]  Rossato, M., Curtale, G., Tamassia, N., Castellucci, M., Mori, L., Gasperini, S., Bazzoni, F. 2012. IL-10-induced microRNA-187 negatively regulates TNF-α, IL-6, and IL-12p40 production in TLR4-stimulated monocytes. Proceedings of the National Academy of Sciences of the United States of America, 109(45), E3101-E3110.
[9]  Skelly D.T., Hennessy E., Dansereau M-A., Cunningham C., 2013. Correction: A Systematic Analysis of the Peripheral and CNS Effects of Systemic LPS, IL-1β, TNF-α and IL-6 Challenges in C57BL/6 Mice. PLoS ONE 8(12).
[10]  An, X., Lee, S. G., Kang, H., Heo, H.J., Cho,Y.S., Kim, D.O., 2016. Antioxidant and Anti-inflammatory Effects of Various Cultivars of Kiwi Berry (Actinidia arguta) on Lipopolysaccharide-stimulated RAW 264.7 Cells. J Microbiol Biotechnol. 28; 26(8):1367-74.
[11]  Jeong, Y.Y., Ryu, J.H., Shin, J.H., Kang, M.J., Kang, J.R., Han, J., Kang, D. 2016. Comparison of Anti-Oxidant and Anti-Inflammatory Effects between Fresh and Aged Black Garlic Extracts. Molecules (Basel, Switzerland). 21, 430.
[12]  Jin, S.E., Kim, O.S., Yoo, S., Seo, C., Kim, Y., Shin, H., Jeong, S. 2016. “Anti-inflammatory effect and action mechanisms of traditional herbal formula Gamisoyo-san in RAW 264.7 macrophages.” BMC Complement Altern Med, 16: 219.
[13]  Chen, C.-C., Lin, M.-W., Liang, C.-J., Wang, S.-H., 2016. The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages. PLoS ONE, 11(7), e0158662.
[14]  Jurenka J.S., 2009. Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research, Altern Med Rev. 14(2): 141-53.
[15]  Menon V.P., Sudheer A.R., 2007. Antioxidant and anti-inflammatory properties of curcumin, Adv Exp Med Biol. 595: 105-125.
[16]  Mashhadi, N. S., Ghiasvand, R., Askari, G., Feizi, A., Hariri, M., Darvishi, L., Hajishafiee, M. 2013. Influence of Ginger and Cinnamon Intake on Inflammation and Muscle Soreness Endued by Exercise in Iranian Female Athletes. Int J Prev Med. 4 (Suppl 1), S11-S15.
[17]  Hong, J.-W., Yang, G.-E., Kim, Y. B., Eom, S. H., Lew, J.-H., Kang, H., 2012. Anti-inflammatory activity of cinnamon water extract in vivo and in vitroLPS-induced models. BMC Complementary and Alternative Medicine, 12, 237.
[18]  Joshi, K., Awte, S., Bhatnagar, P., Walunj, S., Gupta, R., Joshi, S., Sabharwal, S., Bani, S. Padalkar, A.S., 2010. Cinnamomum zeylanicum extract inhibits proinflammatory cytokine TNFµ: in vitro and in vivo studies, Research In Pharmaceutical Biotechnology, 2(2), 14-21.
[19]  Rao, P.V., Gan, S.H., 2014. Cinnamon: A Multifaceted Medicinal Plant, Evidence-Based Complementary and Alternative Medicine, Volume 2014, Article ID 642942, 12.
[20]  Muhammad, J. S., Zaidi, S. F., Shaharyar, S., Refaat, A., Usmanghani, K., Saiki, I., Sugiyama, T. 2015. “Anti-inflammatory Effect of Cinnamaldehyde in Helicobacter pylori Induced Gastric Inflammation.” Biological and Pharmaceutical Bulletin 38(1): 109-115.
[21]  Zhu, W., Wu, Y., Meng, Y.-F., Wang, J.-Y., Xu, M., Tao, J.-J., Lu, J., 2015. Effect of curcumin on aging retinal pigment epithelial cells. Drug Design, Development and Therapy, 9, 5337-5344.
[22]  Qabaha, K., Abu Ras, S., Abbadi, J., Al-Rimawi, F., 2016. Ant-inflammatory of Eucalyptus spp. and Pistascia lentiscus leaf Extracts, Afr J Tradit Complement Altern Med. 13(5):1-6.
[23]  Avelar-Freitas, B. A., Almeida, V. G., Pinto, M. C. X., Mourão, F. A. G., Massensini, A. R., Martins-Filho, O. A., Brito-Melo, G. E. A., 2014. Trypan blue exclusion assay by flow cytometry. Brazilian Journal of Medical and Biological Research, 47(4), 307-3015.
[24]  Godoy, M. E. Rotelli, A., Pelzer, L., Tonn, C. E., 2000, Antiinflammatory Activity of Cinnamic Acid Esters, Molecules, 5, 547-548.
[25]  Liao, J.-C., Deng, J.-S., Chiu, C.-S., Hou, W.-C., Huang, S.-S., Shie, P.-H., Huang, G.-J., 2012. Anti-Inflammatory Activities of Cinnamomum cassia Constituents In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine: eCAM, 2012, 429320.
[26]  Jacob, A., Wu, R., Zhou, M., Wang, P., 2007. Mechanism of the Anti-inflammatory Effect of Curcumin: PPAR-γ Activation, PPAR Research, Volume 2007, Article ID 89369, 5.