Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2017, 5(9), 642-648
DOI: 10.12691/jfnr-5-9-3
Open AccessArticle

Development of a Simple Method for Determination of Anti-cancer Component of Indole-3-carbinol in Cabbage and Broccoli

Zhansheng Li1, , Xiaochun Wei2, Lingyun Li1, Yumei Liu1, Zhiyuan Fang1, Limei Yang1, Mu Zhuang1, Yangyong Zhang1 and Honghao Lv1

1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China

2Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, China

Pub. Date: August 12, 2017

Cite this paper:
Zhansheng Li, Xiaochun Wei, Lingyun Li, Yumei Liu, Zhiyuan Fang, Limei Yang, Mu Zhuang, Yangyong Zhang and Honghao Lv. Development of a Simple Method for Determination of Anti-cancer Component of Indole-3-carbinol in Cabbage and Broccoli. Journal of Food and Nutrition Research. 2017; 5(9):642-648. doi: 10.12691/jfnr-5-9-3


Indole-3-carbinol is a potential anti-cancer agent, and it has been found rich in Brassica vegetables. However, there was few good extraction method of indole-3-carbinol in cabbage and broccoli. In this work, the wavelength and the ration of mobile phase in determination method, as well as different temperature and pH values in extraction method were all investigated some cultivars of cabbage and broccoli were collected and validated the determination method. A sensitive and rapid high-performance liquid chromatography method has been established and used for the determination of indole-3-carbinol in cabbage (Brassica oleracea L. var. capitata) and broccoli (Brassica oleracea L. var. italica). The method was proved to be sensitive, selective, rapid and reproducible, with a good recovery of 99.25%. There was a fast retention time (4.88 min) and good linearity (R2=0.9991) in the system. Meanwhile, significant differences of indole-3-carbinol contents were detected among all the materials (P<0.05). Finally a simple extracting method of indole-3-carbinol was established, at the same time, the determination method was shown accurate, reliable and stable. So the determination method could be proposed application in medical and industrial area.

indole-3-carbinol cabbage broccoli HPLC anti-cancer

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