Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2017, 5(6), 370-378
DOI: 10.12691/jfnr-5-6-3
Open AccessArticle

Influence of Cookies Formulation on the Formation of Acrylamide

Wen Chieh Sung1, and Chih Ya Chen1

1Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan

Pub. Date: May 17, 2017

Cite this paper:
Wen Chieh Sung and Chih Ya Chen. Influence of Cookies Formulation on the Formation of Acrylamide. Journal of Food and Nutrition Research. 2017; 5(6):370-378. doi: 10.12691/jfnr-5-6-3


This study examined the effects of cookie ingredients and cookie formulation without adding leavening agents, sodium bicarbonate and ammonium bicarbonate, sucrose, glucose, fructose and adding chitosan on the pH, water activity, browning index, reducing sugar of cookies and the formation of acrylamide. High browning index value (218) of control cookies was found, as compared to a combination of ingredients, model cookies (33), cake flour and water during 15 min baking. Higher browning index value (83) of model cookies with the addition of shortening did not show a high acrylamide concentration. The highest mitigation (55.2%) of acrylamide formation was obtained by removing ammonium bicarbonate in control cookie formulation. The formation of acrylamide showed a positive correlation with the cookie baking time. The baking time significantly influences the physicochemical properties of cookies. Ammonium bicarbonate was the most effective ingredient in terms of causing the formation of acrylamide in cookie formulation. The addition of chitosan was also able to mitigate the formation of acrylamide during baking.

acrylamide cookie reducing sugar sodium bicarbonate ammonium bicarbonate

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[1]  Gokmen V, Acar OC, Serpen A, Morales FJ. Effect of leavening agents and sugars on the formation of hydroxymethylfurfural in cookies during baking. Eur Food Res Technol 226: 1031-1037, 2008.
[2]  Lineback DR, Coughlin JR, Stadler RH. Acrylamide in : A review of the science and future considerations. The Annual Review of Food Science and Technology 3: 15-35, 2012.
[3]  Tareke E, Rydberg R, Karlsson P, Eriksson S, Tornqvist M. Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J Agr Food Chem 50: 4998-5006, 2002.
[4]  Yasahura A, Tanaka Y, Hengel M, Shibamoto, T. Gas chromatographic investigation of acrylamide formation in browning model system. J Agr Food Chem 51: 3999-4003, 2003.
[5]  Zyzak D, Sanders RA, Stojanovic M, Tallmadge DH, Loye Eberhart B, Ewald DK, Gruber DC, Morsch TR, Strothers MA, Rizzi GP, Villagran MD. Acrylamide formation mechanism in heated foods. J Agr Food Chem 51: 4782-7, 2003.
[6]  Tareke E, Rydberg P, Karlsson P, Eriksson S, Tornqvist M. Acrylamide: a cooking carcinogen? Chemical Research in Toxicology 13: 517-522, 2000.
[7]  Becalski A, Lan BPY, Lewis D, Seaman SW. Acrylamide in foods: occurrence, sources, and modeling. J Agr Food Chem 51: 802-808, 2003.
[8]  Acar OC, Pollio M, Monaco RD, Fogliano V, Gokmen V. Effect of calcium on acrylamide level and sensory properties of cookies. Food Bioprocess Tech 5: 519-526, 2012.
[9]  Mottram DS, Wedzicha BL, Dodson AT. Acrylamide is formed in the Miallard reaction. Nature 419: 448-449, 2002.
[10]  Jung MY, Choi DS, Ju JW. A novel technique for limitation of acrylamide formation in fried and baked corn chips and in French fries. J Food Sci 68: 1287-1290, 2003.
[11]  Lindsay RC, Jang S. Chemical intervention strategies for substantial suppression of acrylamide formation in fried potato products. Adv Exp Med Biol 561: 393-404, 2005.
[12]  Gokmen V, Senyuva HZ. Acrylamide formation is prevented by diavalent cations during the Maillard reaction. Food Chem 103: 196-203, 2007.
[13]  Gokmen V, Acar OC, Koksel H, Acar J. Effects of dough formula and baking conditions on acrylamide and hydroxymethylfurfural formation in cookies. Food Chem 2007; 104: 1136-1142.
[14]  Gokmen V, Akbudak B, Serpen A, Acar J, Turan ZM, Eris A. Effects of controlled atmosphere storage and low-dose irradiation on potato tuber components affecting acrylamide and color formations upon frying. Eur Food Res Technol 224: 681-687, 2007.
[15]  Amrein TM, Schonbachler B, Escher F, Amado R. Acrylamide in gingerbread: critical factors for formation and possible ways for reduction. J Agr Food Chem 52: 4282-4288, 2004.
[16]  Pedreschi F, Kaack K, Granby K. The effect of asparaginase on acrylamide formation in French fries. Food Chem 109: 386-392, 2008.
[17]  Ciesarova Z, Kiss E, Boegl P. Impact of L-asparaginase on acrylamide content in potato products. J Food Nutr Res 45: 141-146, 2006.
[18]  Brathen E, Kita A, Knutsen SH, Wicklund T. Addition of glycine reduces the content of acrylamide in cereal and potato products. J Agr Food Chem 53: 3259-3264, 2005.
[19]  Biedermann M, Grob K. Model studies on acrylamide formation in potato, wheat flour and corn starch; ways to reduce acrylamide contents in bakery ware. Mitteilungen aus Lebensmitteluntersuchung und Hygiene 94: 406-422, 2003.
[20]  Li SL, Lin J, Chen XM. Effect of chitosan molecular weight on the functional properties of chitosan-maltose Maillard reaction products and their application to fresh-cut Typha latifolia L. Carybohydr Polym 102: 682-90, 2014.
[21]  AACC. Approved methods of the American Association of Cereal Chemists. 10th ed. St. Paul, Minnesota, 2000.
[22]  Hwang JY, Sung WC, Shyu YS. Effect of mulberry lees addition on dough mixing characteristics and the quality of mulberry toast. J Mar Sci Technol-TA 16: 103-108, 2008.
[23]  Ilyina AV, Tikhonov VE, Albulov AI, Varlamov VP. Enzymic preparation of acid-free-water soluble chitosan. Process Biochem 35: 536-568, 2000.
[24]  Gokmen V, Sugut H. A non-contact computer vision based analysis of color in foods. Int J Food Eng 3: 1-13, 2007.
[25]  Belitz HD, Grosch W, Schieberle P. Carbohydrates. In: Food Chemistry. Berlin: Springer; 2009, p. 248-339.
[26]  Clawson AR, Taylor AJ. Chemical changes during cooking of wheat. Food Chem 47: 337-343, 1993.
[27]  Hamlet CG, Sadd PA. Effects of yeast stress and pH on 3-monchloropropanediol (3-MCPD)-producing reactions in model dough systems. Food Addit Contam 22: 616-623, 2005.
[28]  Levin RA, Ryan SM. Determining the effect of calcium cations on acrylamide formation in cooked wheat products using a model system. J Agr Food Chem 57: 6823-6829, 2009.
[29]  Ameur LA, Mathieu O, Lalanne V, Trystram G, Birlouez-Aragon I. Comparison of the effects of sucrose and hexose on furfural formation and browning in cookies baked at different temperatures. Food Chem 101: 1407-16, 2007.
[30]  Katz EE, Labuza TP. Effect of water activity on the sensory crispness and mechanical formation of snack food products. J Food Sci 46: 403-9, 1980.
[31]  Friedman M, Mottram D. Advances in experimental medicine and biology. Chemistry and Safety of Acrylamide in Food. New York: Springer; 2005; p. 205-22.
[32]  Gokmen V, Senyuva HZ. Effects of some cations on the formation of acrylamide and furfurals in glucose-asparagine model system. Eur Food Res Technol 225: 815-20, 2007.
[33]  Wade P. Biscuits, cookies and crackers, volume 1: The principles of the craft. New York: Elsevier Applied Science; 1988, p. 51-81.