Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2017, 5(3), 156-159
DOI: 10.12691/jfnr-5-3-3
Open AccessArticle

Identification of Molecular Biomarkers for Food Quality Assessment of Oysters Exposed to Hypoxia

So Yun Park1, Jinik Hwang1, Juyun Lee2, Youngjae Chung3, Donggiun Kim4 and Taek-Kyun Lee1,

1South Sea Environment Research Department, Korea Institute of Ocean Science and Technology,Geoje,Republic of Korea

2Korea Marine Environment Management Corporation, Seoul, Republic of Korea

3Department of Life Science and Biotechnology, Shingyeong University, Hwaseong, Republic of Korea

4Department of Biological Science, Silla University, Busan, Republic of Korea

Pub. Date: March 03, 2017

Cite this paper:
So Yun Park, Jinik Hwang, Juyun Lee, Youngjae Chung, Donggiun Kim and Taek-Kyun Lee. Identification of Molecular Biomarkers for Food Quality Assessment of Oysters Exposed to Hypoxia. Journal of Food and Nutrition Research. 2017; 5(3):156-159. doi: 10.12691/jfnr-5-3-3

Abstract

Hypoxia, corresponding to a low oxygen concentration of less than 2.8 mg O2/L (91.4 mM), may cause serious problems in marine environments. In this study, we applied differential display PCR and fatty acid analysis to investigate molecular biomarkers for assessing hypoxic effects using the oyster, Crassostrea gigas, as a model organism. Oysters were exposed to normoxic (7.6 mg O2/L) or hypoxic (1.8 mg O2/L) concentrations of dissolved oxygen for 2 days. We found that glutamine synthetase (GS) gene expression decreased and glutathione S-transferase (GST) gene expression increased in oysters exposed to hypoxia. In addition, linoleic acid content significantly decreased following hypoxic exposure compared with controls. Collectively, our findings indicate that GS and GST expression levels and linoleic acid content are potentially good biomarkers for analyzing the effects of hypoxia.

Keywords:
hypoxia biomarkers DD-PCR fatty acid oyster

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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