Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2013, 1(5), 87-91
DOI: 10.12691/jfnr-1-5-2
Open AccessArticle

Purification of an Antiproliferative Lectin from Erophaca Baetica (Leguminosae) Seeds

Cristina Megías1, Isabel Cortés-Giraldo1, Julio Girón-Calle1, Manuel Alaiz1 and Javier Vioque1,

1Instituto de la Grasa (C.S.I.C.), Avda Padre García Tejero, Sevilla, SPAIN

Pub. Date: September 24, 2013

Cite this paper:
Cristina Megías, Isabel Cortés-Giraldo, Julio Girón-Calle, Manuel Alaiz and Javier Vioque. Purification of an Antiproliferative Lectin from Erophaca Baetica (Leguminosae) Seeds. Journal of Food and Nutrition Research. 2013; 1(5):87-91. doi: 10.12691/jfnr-1-5-2

Abstract

A lectin has been purified from the seeds of Erophaca baetica, an endemic legume of the Mediterranean Region. The protein has been purified from an albumin extract by gel filtration chromatography, after realization that affinity chromatography using Sephadex G-50 did not retain any proteins. Characterization of this protein shows that it is a 60 kDa homodimeric glycoprotein with 235 mg sugars / g protein, and two 30 kDa subunits. Its amino acid composition is similar to those reported for the lectins of other related legumes such as Astragalus mongholicus. It agglutinates trypsinized erythrocytes, and inhibits proliferation of human leukemic THP-1 cells. Thus, this novel lectin may be of interest from a functional point of view due to its antiproliferative activity.

Keywords:
Erophaca baetica lectin antiproliferative THP-1 cells seed proteins legumes

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