Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Journal of Food and Nutrition Research. 2016, 4(11), 736-741
DOI: 10.12691/jfnr-4-11-6
Open AccessArticle

Antioxidant Activity and Phytochemical Composition of Solanum corymbiflorum Fractions (Leaves and Fruits)

Mariana Piana1, , Roberta da silva Jesus1, Aline Augusti Boligon1, Sílvio Terra Stefanello2, Thiele Faccim de Brum1, Camilla Filippi dos Santos Alves1, Natalia Jank Mossmann1, Bianca Vargas Belke1, Félix Alexandre Antunes Soares2, Sara Marchesan de Oliveira3 and Liliane de Freitas Bauermann1

1Post-Graduate Program in Pharmaceutical Sciences, Universidade Federal de Santa Maria, Santa Maria, Brazil

2Post-Graduate Program in Biochemical Toxicology, Universidade Federal de Santa Maria, Santa Maria, Brazil

3Post-graduate Program in Biological Sciences, Universidade Federal de Santa Maria, Santa Maria, Brazil

Pub. Date: November 17, 2016

Cite this paper:
Mariana Piana, Roberta da silva Jesus, Aline Augusti Boligon, Sílvio Terra Stefanello, Thiele Faccim de Brum, Camilla Filippi dos Santos Alves, Natalia Jank Mossmann, Bianca Vargas Belke, Félix Alexandre Antunes Soares, Sara Marchesan de Oliveira and Liliane de Freitas Bauermann. Antioxidant Activity and Phytochemical Composition of Solanum corymbiflorum Fractions (Leaves and Fruits). Journal of Food and Nutrition Research. 2016; 4(11):736-741. doi: 10.12691/jfnr-4-11-6


The antioxidant activity and phenolic compounds of the chloroform (CHCl3), ethyl acetate (AcOEt) and n-butanol (n-BuOH) fractions from Solanum corymbiflorum leaves and fruits were evaluated. The AcOEt fraction of the leaves presented the highest content of total polyphenols (114.00 mg GAE/g) and the best antioxidant capacity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test (IC50 = 31.90 µg/mL). For the fruits, the same fraction exhibited the highest content of phenolics (99.77 mg GAE/g) and best results in the DPPH test (IC50 = 141.47 µg/mL). In relation to 2′,7′-dichlorofluorescein diacetate (DCFH-DA) and thiobarbituric acid reactive substances (TBARS) assays, the CHCl3 fraction of leaves and fruits showed better results than the other samples analyzed. Besides of the phenolic compounds, the alkaloids contributed in the activity. Rutin, chlorogenic and caffeic acids quantified by HPLC are some of phenolic compounds responsible by this activity S. corymbiflorum can be a promising source of natural antioxidants. However, more in vivo studies are required to stimulate the consumption and its other potentialities.

Solanum corymbiflorum antioxidant activity HPLC chlorogenic acid Cyphomandra corymbiflora

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Boligon, A.A., Pereira, R.P., Feltrin, A.C., Machado, M.M., Janovik, V., Rocha, JBT. and Athayde, M.L. Antioxidant activities of flavonol derivates from the leaves and stem bark of Scutia buxifolia Reiss. Bioresour Technol. 100, 6592-6598. 2009.
[2]  Erkan N. Antioxidant activity and phenolic compounds of fractions from Portulaca oleracea L. Food Chem. 133, 775-781, 2012.
[3]  Moo-Huchin, V.M., Estrada-Mota, I., Estrada-León, R., Cuevas-Glory, L., Ortiz-Vazquez, E., Vargas, M.L.V., Betancur-Ancona, D. and Sauri-Duch, E. Determination of some physicochemical characteristics, bioactive compounds and antioxidant activity of tropical fruits from Yucatan, Mexico. Food Chem. 152, 508-515, 2015.
[4]  Choi, Y. and Lee, J. Antioxidant and antiproliferative properties of a tocotrienol- rich fraction from grape seeds. Food Chem. 114, 1386-1390, 2009.
[5]  Almeida, M.M.B., Sousa, P.H.M., Arriaga, Â.M.C., Prado, G.M., Magalhães, C.E.C., Maia, G.A. and Lemos, T.L.G. Bioactive compounds and antioxidant activity of fresh exotic fruits from northeastern Brazil. Food Res Int. 44, 2155-2159, 2011.
[6]  Soares, E.L.C. and Mentz, L.A. As espécies de solanum subgênero bassovia seção pachyphylla (= cyphomandra mart. ex sendtn. - solanaceae) no Rio Grande do Sul, Brasil. Pesquisas botânica. 57, 231-254, 2006.
[7]  Piana, M., Camponogara, C., Boligon, A.A., Machado M. M, Brum, T.F., Oliveira, S.M. and Freitas Bauermann, L. 2016. Topical anti-inflammatory activity of Solanum corymbiflorum leaves. J Ethnopharmacol. 179, 16-21, 2016.
[8]  Keller, H.A. and Prance, G.T. Etnobotánica de las especies de Solanum, Subgénero Bassovia, sección Pachyphylla (Solanaceae) De Misiones, Argentina. Bonplandia. 21, 45-54, 2012.
[9]  Kinupp, V.F. Plantas alimentícias não convencionais da região metropolitana de Porto Alegre. Federal University of the Rio Grande do Sul (Doctoral Thesis), 2007.
[10]  Chandra, S. and Mejia, E.G. 2004. Polyphenolic compounds, antioxidant capacity, and quinine reductase activity of an aqueous extract of Ardisia compressa in comparison to mate (Ilex paraguariensis) and green (Camellia sinensis) teas. J Agric Food Chem. 52, 3583-3589, 2004.
[11]  Woisky, R.G. and Salatino, A. Analysis of propolis: Some parameters and procedures for chemical quality control. J Apicult Res. 37, 99-105. 1998.
[12]  Morrison, I.M., Asiedu, E.A., Stuchbury, T. and Powell, A.A. Determination of lignin and tannin contents of Cowpea seed coats. Ann Bot Lond. 76, 287-290, 1995.
[13]  Sreevidya, N. and Mehrotra, S. Spectrophotometric method for estimation of alkaloids precipitable with Dragendorff’s reagent in plant materials. J AOAC Int. 86. 1124-1127, 2003.
[14]  Piana, M., Boligon, A.A., Brum, T.F., Brum, T.F., Zadra, M., Belke, B.V., Froeder, A.L., Frohlich, J.K., Nunes, L.T., Pappis. L., Boligon, A.A. and Athayde, M.L. Phytochemical analysis and antioxidant capacity of Tabernaemontana catharinensis A. DC. Fruits and branches. An Acad Bras Ciênc. 86, 881-888, 2014.
[15]  Choi, C.W., Kim, S.C., Hwang, S.S., Choi, B.K., Ahn, H.J., Lee, M.Y., Park, S.H. and Kim, S.K. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci 163, 1161-1168, 2002.
[16]  Myrhe, O., Andersen, J.M., Aarnes, H. and Fonnum, F. 2003. Evaluation of the probes 2′,7′-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation. Biochem Pharmacol, 65, 1575-1582, 2003.
[17]  Ohkawa, H., Ohishin, N. and Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95, 351-358, 1979.
[18]  Hari, R., Vasuki, R., Anbu, J., Muralikrishna, B., Manasa, G. and Geethanjali. Comparative free radical scavenging and analgesic activity of ethanolic leaves and stem extracts of Solanum nigrumJ Med Sci. 13, 327-336, 2013.
[19]  Zadra, M., Piana, M., Brum, T.F., Boligon, A.A., Freitas, R.B., Machado, M.M., Stefanello, S.T., Soares, F.A.A. and Athayde, M.L. Antioxidant activity and phytochemical composition of the leaves of Solanum guaraniticum A. St.-Hil. Molecules. 17, 12560-12574, 2012.
[20]  Rice-evans, C.A., Miller, N.J. and Paganga, G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 20, 933-956, 2006.
[21]  Boligon, A.A., Freitas, R.B., Brum, T.F., Piana, M., Belke, B.V., Rocha, J.B.T. and Athayde, M.L. Phytochemical constituents and in vitro antioxidant capacity of Tabernaemontana catharinensis A. DC. Free Radicals and Antioxidants. 3, 77-80, 2013.
[22]  Sudha, G., Priya, M.S, Shree, R.I. and Vadivukkarasi, S. Antioxidant activity of ripe pepino fruit (Solanum muricatum Aiton). Int J Pharm Pharm Sci. 3, 257-61, 2011.
[23]  Jung, H., Min, B.S., Yokozawa, T., Lee, J.H., Kim, Y.S. and Choi, J.S. Anti-Alzheimer and antioxidant activities of Coptidis Rhizoma alkaloids. Biol Pharm Bull. 32, 1433-1438, 2009.
[24]  Koduru S, Jimoh FO, Grierson, D.S. and Afolayan, A.J. Antioxidant activity of two steroid alkaloids extracted from Solanum aculeastrum. J Pharmacol Toxicol. 2, 160-167, 2007.
[25]  Yin, H., Xu, L. and Porter, N.A. Free radical lipid peroxidation: Mechanisms and analysis. Chem Rev. 111, 5944-5972, 2011.
[26]  Boligon, A.A., Machado, M.M. and Athayde, M.L. Technical Evaluation of Antioxidant Activity. Med chem. 4, 517-522, 2014.
[27]  Selvendiran, K., Singh, J.P.V., Krishnan, K.B. and Sakthisekaran, D. 2003. Cytoprotective effect of piperine against benzowaxpyrene induced lung cancer with reference to lipid peroxidation and antioxidant system in Swiss albino mice. Fitoterapia, 74, 109-115, 2003.
[28]  Račková, L., Májeková, M., Košt’álová, D. and Štefek, M. Antiradical and antioxidant activities of alkaloids isolated from Mahonia aquifolium. Structural aspects. Bioorgan Med Chem.12, 4709-4715, 2004.