Antioxidant Activity and Phytochemical Composition of Solanum corymbiflorum Fractions (Leaves and Fruits)

Mariana Piana^1, , Roberta da silva Jesus¹, Aline Augusti Boligon¹, Sílvio Terra Stefanello², Thiele Faccim de Brum¹, Camilla Filippi dos Santos Alves¹, Natalia Jank Mossmann¹, Bianca Vargas Belke¹, Félix Alexandre Antunes Soares², Sara Marchesan de Oliveira³ and Liliane de Freitas Bauermann¹

¹Post-Graduate Program in Pharmaceutical Sciences, Universidade Federal de Santa Maria, Santa Maria, Brazil

²Post-Graduate Program in Biochemical Toxicology, Universidade Federal de Santa Maria, Santa Maria, Brazil

³Post-graduate Program in Biological Sciences, Universidade Federal de Santa Maria, Santa Maria, Brazil

Cite this paper:
Mariana Piana, Roberta da silva Jesus, Aline Augusti Boligon, Sílvio Terra Stefanello, Thiele Faccim de Brum, Camilla Filippi dos Santos Alves, Natalia Jank Mossmann, Bianca Vargas Belke, Félix Alexandre Antunes Soares, Sara Marchesan de Oliveira and Liliane de Freitas Bauermann. Antioxidant Activity and Phytochemical Composition of Solanum corymbiflorum Fractions (Leaves and Fruits). Journal of Food and Nutrition Research. 2016; 4(11):736-741. doi: 10.12691/jfnr-4-11-6

Abstract

The antioxidant activity and phenolic compounds of the chloroform (CHCl₃), ethyl acetate (AcOEt) and n-butanol (n-BuOH) fractions from Solanum corymbiflorum leaves and fruits were evaluated. The AcOEt fraction of the leaves presented the highest content of total polyphenols (114.00 mg GAE/g) and the best antioxidant capacity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test (IC₅₀ = 31.90 µg/mL). For the fruits, the same fraction exhibited the highest content of phenolics (99.77 mg GAE/g) and best results in the DPPH test (IC₅₀ = 141.47 µg/mL). In relation to 2′,7′-dichlorofluorescein diacetate (DCFH-DA) and thiobarbituric acid reactive substances (TBARS) assays, the CHCl₃ fraction of leaves and fruits showed better results than the other samples analyzed. Besides of the phenolic compounds, the alkaloids contributed in the activity. Rutin, chlorogenic and caffeic acids quantified by HPLC are some of phenolic compounds responsible by this activity S. corymbiflorum can be a promising source of natural antioxidants. However, more in vivo studies are required to stimulate the consumption and its other potentialities.

Keywords:
Solanum corymbiflorum antioxidant activity HPLC chlorogenic acid Cyphomandra corymbiflora

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