Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(11), 703-708
DOI: 10.12691/jfnr-4-11-1
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Proximate Composition and Micronutrient Mineral Profile of wild Ganoderma lucidum and Four Commercial Exotic Mushrooms by ICP-OES and LIBS

Sumaira Sharif1, 2, , Ghulam Mustafa3, Hira Munir2, Connie M. Weaver1, Yasir Jamil4 and Muhammad Shahid3

1Nutrition Science Department, Purdue University West Lafayette, USA

2Department of Biochemistry and Molecular Biology, University of Gujrat, 50700 Gujrat, Pakistan

3Department of Biochemistry, University of Agriculture Faisalabad-38040, Pakistan

4Department of Physics, University of Agriculture Faisalabad-38040, Pakistan

Pub. Date: October 17, 2016

Cite this paper:
Sumaira Sharif, Ghulam Mustafa, Hira Munir, Connie M. Weaver, Yasir Jamil and Muhammad Shahid. Proximate Composition and Micronutrient Mineral Profile of wild Ganoderma lucidum and Four Commercial Exotic Mushrooms by ICP-OES and LIBS. Journal of Food and Nutrition Research. 2016; 4(11):703-708. doi: 10.12691/jfnr-4-11-1


Edible mushrooms are excellent food that can be incorporated into well balanced diets due to their low content of fat and energy, high content of dietary fibers and proteins. Proximate composition of mushrooms also varies within and among species due to agro-climate conditions and environmental factors. The current study was designed to analyze proximate composition and mineral profile of one local wild Ganoderma lucidum, two commercial local cultivated mushrooms species i.e. Pleurotus ostreatus and Vovoriella volvacea, and two commercial exotic mushrooms i.e. Lentinus edodes and Hericium erinaceus for their nutritive values. Minerals were analyzed by Inductivity Coupled Plasma Optical Emission Spectrometry (ICP-OES). Selected mushrooms were also analyzed by laser induced break down spectroscopy (LIBS) to identify any harmful element(s) present in these mushrooms. Proximate analysis showed that crude protein ranged from 15.04-24.8%, crude fat 0.53-2.02%, Fiber 6.11-54.12%, Ash 2.0-9.99% and total carbohydrates varied in a range of 65.34-82.47% on dry weight basis. Ca, Mg, Na, Zn, P and K were in elevated concentration. Al, B, Cu, Li and Mn were in the range of 2.5-8.1, 0.4-6.1, 0.9-1.4, 0.2-1.4, 0.4-1.3 mg/100 g on dry weight basis respectively. As, Ba and Se were in lower concentration whereas Pb, Cd, Mo, Be, Sn and Co were below detectable limits. LIBS also revealed some elements like, titanium, barium, calcium, iodine, carbon and hydrogen. The selected local mushrooms are safe for consumption, in accordance with the permissible tolerance limit of the toxic metals.

mineral analysis proximate composition Pleurotus ostreatus Vovoriella volvacea

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[1]  Adejumo, T.O. and Awosanya, O.B. “Proximate and mineral composition of four edible mushroom species from South Western Nigeria”. African Journal of Biotechnology, 4, 1084-1088, 2005.
[2]  AOAC. 1995. Official Methods of Analysis. Association of Official Analytical Chemists. 16th Ed., Arlington, VA.
[3]  Brzostowski, A., Bielawski, L., Orlikowska, A., Plichta, S. and Falandysz, J. “Instrumental analysis of metals profile in poison pax (Paxillus involutus) collected at two sites in Bory Tucholskie”. Chemical Anal-Warsaw, 54, 1297-1308, 2009.
[4]  Colak, A., Faiz, Z. and Sesli, E. “Nutritional composition of some wild edible mushrooms”. Turkish Journal of Biochemistrt, 34, 25-31, 2009.
[5]  Falandysz, J., and Brzostowski, A. “Mercury and its bioconcentration factors in Poison Pax (Paxillus involutus) from various sites in Poland”. Journal of Environmental Sciences Health, Part A: Toxic/Hazar Substances and Environmental Engineering, 42, 1095-1100, 2007.
[6]  Falandysz, J. “Selenium in edible mushrooms”. Journal of Environmental Sciences Health, 26, 256-299, 2008.
[7]  Genccelep, H., Uzun, Y., Tuncturk, Y. and Demirel, K.”Determination of mineral contents of wild grown edible mushrooms”. Food Chemistry, 113, 133-1036, 2009.
[8]  Hung, P.V. and Nhi, N.N.Y. “Nutritional composition and antioxidant capacity of several edible mushrooms grown in the Southern Vietnam”. International Food Research Journal, 19, 611-615, 2012.
[9]  Jonathan, S.G. and Awotona, F.E. “Studies on Antimicrobial Potentials of three Ganoderma species”. African Journal of Biomedical Research, 13, 133-139, 2010.
[10]  Keen, C.L., Uriu-Adams, J.Y., Ensuma, J.L., and Gershwin, M.E. Trace elements/ minerals and immunity. In: Gershin ME, Nestel P, Keen CL, (Eds.), Handbook of nutrition and immunity. Humana Press, 2004, Totowa, NJ, (pp. 117-140). 2004.
[11]  Koyyalamudi, S.R., Jeong, S.C., Manavalan, S., Vysetti, B., and Pang, G. “Micronutrient mineral content of the fruiting bodies of Australian cultivated Agaricus bisporus white button mushrooms”. Journal of Food Compositition and Analysis. 31:109-114, 2013.
[12]  Kuldo, E., Jarzynska, J. Z., Gucia, M. & Falandysz, J. “Mineral constituents of edible parasol mushroom (Scop. ex Fr.) Sing and soils beneath its fruiting bodies collected from a rural forest area”. Chemical Papers, 68, 484-492, 2014.
[13]  Mallikarjuna, S.E., Ranjini, A., Haware, D.J., Vijayalakshmi, M.R., Shashirekha, M.N. and Rajarathnam, S. “Mineral Composition of Four Edible Mushrooms”. Journal of Chemical education. 2013.
[14]  Mattila, P., Salo-Vaananen, P., Konko, K., Aro, H., and Jalava, T. “Basic composition and amino acid contents of mushrooms cultivated in Finland”. Journal of Agriculture Food Chemistry, 50, 6419-6422, 2002.
[15]  McDowell, L. R. “Minerals in Animal and Human Nutrition, 2nd ed. Amsterdam”, 2003. The Netherlands, Elsevier.
[16]  Moore, D. and Chi, S.W. “Funi products as food (eds) pointing, S.B and Hyde, K.O. Int Bio-explotation of filamentous fungi”. Fungi Diversity Research. Lenis, 6, 223-251, 2005.
[17]  Mukhopadhyay, R. and Guha, A.K.”A comprehensive analysis of the nutritional quality of edible mushroom Pleurotus sajor-caju grown in deproteinized whey medium”. LWT- Food Science and Technology, 61, 339-345, 2015.
[18]  Okoro, I. O. and Achuba, F.I. “Proximate and mineral analysis of some wild edible mushrooms”. African Journal of Biotechnology, 11, 7720-7724. 2015.
[19]  Ouzouni, P. K., Petridis, D., Koller, W. D. and Riganakos, K. A. “Nutritional value and metal content of wild edible mushrooms collected from West Macedonia and Epirus, Greece”. Food Chemistry, 115, 1575-1580, 2009.
[20]  Soylak, M., Saracoglu, S., Tuzen, M. and Mendil, D. “Determination of trace metals in mushroom samples from Kayseri, Turkey”. Food Chemistry, 92, 649-652, 2005.
[21]  Tuzen, M., Sesli, E. and Soylak, M. “Trace element levels of mushroom species from East Black Sea region of Turkey”. Food Control, 18, 806-810, 2007.
[22]  Vieira, P.A.F., Gontijo, D.C., Vieira, B.C., Fontes, E.A., de Assuncao, L.S., Leite, J.P.V., Oliveira, M.G.D.A. and Kasuya, M.C.M. ”Antioxidant activities, total phenolics and metal contents in Pleurotus ostreatus mushrooms enriched with iron, zinc or lithium”. LWT-Food Science and Technology, 54, 421-425, 2013.
[23]  Wang, Jia-sheng., Yun-zhang, L., Wei-lin, L., Dong-po, Q. and Ying, T. “Laser induced breakdown spectroscopic technique for analyzing rock and soil sample”. Metallurgical Analysis, 29, 30-34, 2009.
[24]  Wang, X.M., Zhang, J., Wu,L.H., Zhao, Y.L., Li, T., Li, J.Q., Wang, Y.Z. and Liu, H. G. “A mini-review of chemical composition and nutritional value of edible wild-grown mushroom from China”. Food Chemistyr. 151:279-285, 2014.
[25]  Xu, D.X., Lin, J., Duan, Z.M., Wan, Y.P., Bai, B. and Sun, C. “Detection of chemical compositions of wild Lactarius volemus from Yunnan province”. Edible Fungi, 4, 60-61, 2012.
[26]  Zahid, M.K., Barua, S. and Huq, S.M. “Proximate Composition and Mineral Content of Selected Edible Mushroom Varieties of Bangladesh”. Bangladesh Journal of Nutrition, 22, 61-68. 2010.
[27]  Zhang, Y., Cao, Y.R. and Xu, H. “Evaluation of heavy metal contents in some wild edible mushrooms from Panzhihua”. Journal of Sichuan University, 49, 246-252, 2012.