Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(10), 690-698
DOI: 10.12691/jfnr-4-10-9
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Inhibitory Effects and Molecular Mechanism of an Anti-inflammatory Peptide Isolated from Intestine of Abalone, Haliotis Discus Hannai on LPS-Induced Cytokine Production via the p-p38/p-JNK Pathways in RAW264.7 Macrophages

Zhong-Ji Qian1, BoMi Ryu2, Won Sun Park3, IL-Whan Choi4, and Won-Kyo Jung5,

1Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China

2School of Pharmacy, the University of Queensland, Brisbane, Qld 4072, Australia

3Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea

4Department of Microbiology, College of Medicine, Inje University, Busan 633-165, Republic of Korea

5Department of Biomedical Engineering, and Center for Marine-Intergrated Biomedical Technology (BK21 Plus) Pukyong National University, Busan 608-737, Republic of Korea

Pub. Date: October 15, 2016

Cite this paper:
Zhong-Ji Qian, BoMi Ryu, Won Sun Park, IL-Whan Choi and Won-Kyo Jung. Inhibitory Effects and Molecular Mechanism of an Anti-inflammatory Peptide Isolated from Intestine of Abalone, Haliotis Discus Hannai on LPS-Induced Cytokine Production via the p-p38/p-JNK Pathways in RAW264.7 Macrophages. Journal of Food and Nutrition Research. 2016; 4(10):690-698. doi: 10.12691/jfnr-4-10-9


A marine mollusk, abalone (Haliotis discus hannai) is one of the important species in aquaculture industry, but nutraceutical and pharmaceutical benefits of H. discus hannai have been rarely identified and studied. To evaluate beneficial effects of H. discus hannai, an anti-inflammatory peptide (AAIP, abalone anti-inflammatory peptide) was purified from abalone intestines using consecutive HPLC purification system. In tandem MS analysis, the fragmentation results illustrate that the AAIP responsible for the nitric oxide (NO) inhibitory activity (IC50=55.8μM) has amino acid sequence as Pro-Phe-Asn-Glu-Gly-Thr-Phe-Ala-Ser (1175.2 Da). To investigate anti-inflammatory effect of AAIP on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and elucidated the molecular mechanism. The results show that the AAIP peptide suppresses LPS-induced production of nitric oxide (NO) via inducible nitric oxide synthase (iNOS) expression in a dose-dependent manner. It also significantly reduced the gene transcription of proinflammatory cytokines, such as interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α). Furthermore, AAIP significantly suppresses phosphorylation of mitogen-activated protein kinases (MAPKs) such as p-p38 and p-JNK. These results indicated that AAIP inhibits LPS-induced inflammatory response via blocking of MAPK pathway in murine macrophages. Therefore, potent AAIP might suggest possibility for high valuable utilization and application as nutraceutical and therapeutic substances.

marine mollusk inflammation lipopolysaccharide (LPS) nitric oxide nitric oxide synthase mitogen-activated protein kinases (MAPK)

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