Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(9), 610-614
DOI: 10.12691/jfnr-4-9-8
Open AccessArticle

Low Cytotoxic D-mannitol Isolated from the Industrial Wastewater of Agaricus bisporus

Jinmei Lin1, 2, Jiafu Huang1, Junyu Chen3, Zhichao Lin1, Yixin Ou1, Liyun Yao1, Xiufen Zhang1, Qianjin Kang4 and Yutian Pan1,

1Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, China

2College of Chemistry and Environment, Minnan Normal University, Zhangzhou, China

3Dongshan Entry-Exit Inspection and Quarantine Bureau, Zhangzhou, China

4Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai, China

Pub. Date: September 09, 2016

Cite this paper:
Jinmei Lin, Jiafu Huang, Junyu Chen, Zhichao Lin, Yixin Ou, Liyun Yao, Xiufen Zhang, Qianjin Kang and Yutian Pan. Low Cytotoxic D-mannitol Isolated from the Industrial Wastewater of Agaricus bisporus. Journal of Food and Nutrition Research. 2016; 4(9):610-614. doi: 10.12691/jfnr-4-9-8


Agaricus bisporus is an edible mushroom, and mainly made in canned food for storage and trading. However, large amount of industrial wastewater containing various components is generated during the production of canned mushroom. This study describes the method for purification of D-mannitol and evaluation of its bioactivity from the industrial wastewater of A. bisporus. Its structure was confirmed by GC, IR, UV, HPLC, HPLC-MS, DSC analysis. The content of D-mannitol in the concentrated industrial wastewater (≥45°Be') of A. bisporus was determined to more than 32.0%. Comparing with the D-mannitol standard, this purified MN6 had a higher purity and lower cytotoxicity to L929 fibroblast evaluated by MTS assay. These results revealed that the A. bisporus industrial wastewater is a good resource for obtanning natural D-mannitol with high quality.

D-mannitol Agaricus bisporus wastewater lower cytotoxicit

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