Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(9), 610-614
DOI: 10.12691/jfnr-4-9-8
Open AccessArticle

Low Cytotoxic D-mannitol Isolated from the Industrial Wastewater of Agaricus bisporus

Jinmei Lin1, 2, Jiafu Huang1, Junyu Chen3, Zhichao Lin1, Yixin Ou1, Liyun Yao1, Xiufen Zhang1, Qianjin Kang4 and Yutian Pan1,

1Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, China

2College of Chemistry and Environment, Minnan Normal University, Zhangzhou, China

3Dongshan Entry-Exit Inspection and Quarantine Bureau, Zhangzhou, China

4Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai, China

Pub. Date: September 09, 2016

Cite this paper:
Jinmei Lin, Jiafu Huang, Junyu Chen, Zhichao Lin, Yixin Ou, Liyun Yao, Xiufen Zhang, Qianjin Kang and Yutian Pan. Low Cytotoxic D-mannitol Isolated from the Industrial Wastewater of Agaricus bisporus. Journal of Food and Nutrition Research. 2016; 4(9):610-614. doi: 10.12691/jfnr-4-9-8

Abstract

Agaricus bisporus is an edible mushroom, and mainly made in canned food for storage and trading. However, large amount of industrial wastewater containing various components is generated during the production of canned mushroom. This study describes the method for purification of D-mannitol and evaluation of its bioactivity from the industrial wastewater of A. bisporus. Its structure was confirmed by GC, IR, UV, HPLC, HPLC-MS, DSC analysis. The content of D-mannitol in the concentrated industrial wastewater (≥45°Be') of A. bisporus was determined to more than 32.0%. Comparing with the D-mannitol standard, this purified MN6 had a higher purity and lower cytotoxicity to L929 fibroblast evaluated by MTS assay. These results revealed that the A. bisporus industrial wastewater is a good resource for obtanning natural D-mannitol with high quality.

Keywords:
D-mannitol Agaricus bisporus wastewater lower cytotoxicit

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References:

[1]  Liao JH., Study on the crossbreeding using wild strains of Agaricus bisporus. Chin Agric Sci Bull, 29(7), 93-98. 2013.
 
[2]  Huang JF, Ou YX, Yew TW, Liu JN, Leng B, Lin ZC, Su Y, Zhuang YH, Lin JF, Li XM, Xue Y, and Pan YT, Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl4-induced hepatic injury in mice. Int J Biol Macromol, 82, 678-686. 2016,
 
[3]  Hu SQ, Chen ZY, Zou YX, Shi Y, and Liu XM, Determination of Mannitol Content in 15 kinds of Edible Mushrooms by Colorimetry. Modern Food Science and Technology, 26(8), 901-903, 912. 2010,
 
[4]  Commission CP, Pharmacopoeia of the people's Republic of China. Chinese Medical Science Press: Beijing, 2. 2010.
 
[5]  Xie JX, Chang JB, Wang XM, Application of infrared spectroscopy in organic chemistry and pharmaceutical chemistry. Science Press: Beijing, 25-26. 2001.
 
[6]  Zhou S, Xue JJ, Jia W, Zhang JS, Yang Y, and Tang QJ, Alditol content in edible fungal mycelia. Mycosystema, 32(5), 862-867. 2013.
 
[7]  Oddo E, Saiano F, Alonzo G, and Bellini E, An investigation of the seasonal pattern of mannitol content in deciduous and evergreen species of the oleaceae growing in northern Sicily. Ann Bot, 90(2), 239-243. 2002.
 
[8]  Holtz RB, Qualitative and Quantitative Analyses of Free Neutral Carbohydrates in Mushroon Tissues by Gas-Liquid Chromatography and Mass Spectros- copy. J Agric Food Chem, 1(1), 1272-1273. 1971.
 
[9]  Jennings DH, Polyol metabolism in fungi. Adv Microb Physiol, 25, 149-193. 1984.
 
[10]  Hagiwara SY, Takahashi M, Shen Y, Kaihou S, Tomiyama T, Yazawa M, Tamai Y, Sin Y, Kazusaka, A, and Terazawa M, A phytochemical in the edible Tamogi-take mushroom (Pleurotus cornucopiae), D-mannitol, inhibits ACE activity and lowers the blood pressure of spontaneously hypertensive rats. Biosci Biotechnol Biochem, 69(8), 1603-1605. 2005.
 
[11]  Schilte C, Bouzat P, Millet A, Boucheix P, Pernet-Gallay K, Lemasson B, Barbier EL, and Payen J F, Mannitol Improves Brain Tissue Oxygenation in a Model of Diffuse Traumatic Brain Injury. Crit Care Med, 43(10), 2212-2218. 2015.
 
[12]  Soetaert W, Vanhooren PT, and Vandamme EJ, The Production of Mannitol by Fermentation. In Carbohydrate Biotechnology Protocols, Bucke, C., Ed. Humana Press: Totowa, NJ, 261-275. 1999.
 
[13]  Zheng JX, Functional sugar alcohol. Chemical Industry Press: Beijing, 147-180. 2005.
 
[14]  Zhao GH, Wang GB, He DH, and Li JP, Study on the synthesis of D-mannitol by double isomerization method. Chemical Engineer, 3(23), 53-55. 2005.
 
[15]  Kaup B, Bringer-Meyer S, and Sahm H, Metabolic engineering of Escherichia coli: construction of an efficient biocatalyst for D-mannitol formation in a whole-cell biotransformation. Commun Agric Appl Biol Sci, 68, 235-240. 2003.
 
[16]  Wisselink HW, Moers AP, Mars AE, Hoefnagel M H, De Vos WM, and Hugenholtz J, Overproduction of heterologous mannitol 1-phosphatase: a key factor for engineering mannitol production by Lactococcus lactis. Appl Environ Microbiol. 7(13), 1507-1514. 2005.
 
[17]  Ortiz ME, Raya RR, and Mozzi F, Efficient mannitol production by wild-type Lactobacillus reuteri CRL 1101 is attained at constant pH using a simplified culture medium. Appl Microbiol Biotechnol, 99(20), 8717-8729. 2015.
 
[18]  Korakli M, Schwarz E, Wolf G, and Hammes WP, Production of mannitol by Lactobacillus sanfranciscensis. Adv Food Sci, 22(1-2), 1-4. 2000.
 
[19]  Baumchen C, Roth AH, Biedendieck R, Malten M, Follmann M, Sahm H, Bringer-Meyer S, and Jahn D, D-mannitol production by resting state whole cell biotrans-formation of D-fructose by heterologous mannitol and formate dehydrogenase gene expression in Bacillus megaterium. Biotechnol J, 2(11), 1408-1416. 2007.
 
[20]  Von Weymarn FN, Kiviharju KJ, Jaaskelainen ST, and Leisola MS, Scale-up of a new bacterial mannitol production process. Biotechnol Prog, 19(3), 815-821. 2003.
 
[21]  Racine FM, Saha BC, Production of mannitol by Lactobacillus intermedius NRRL B-3693 in fed-batch and continuous cell-recycle fermentations. Process Biochem. 42(12), 1609-1613. 2007.
 
[22]  Carvalheiro F, Moniz P, Duarte LC, Esteves MP, and Girio FM, Mannitol production by lactic acid bacteria grown in supplemented carob syrup. J Ind Microbiol Biot. 38(1), 221-227. 2011.