Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: http://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(9), 558-565
DOI: 10.12691/jfnr-4-9-1
Open AccessArticle

Porphyra tenera Extracts Have Immune Stimulation Activity via Increasing Cytokines in Mouse Primary Splenocytes and RAW264.7 Macrophages

Hee-Bum Kang1, 2, Ji-Hye Song1, 2, Yanghee You3, 4, Jeongjin Park3, 4, Sungmin Kwak1, 2, Yoo-Hyun Lee5, Jeongmin Lee6, Su-il Kim7, Kyung-Chul Choi1, 2, and Woojin Jun3, 4,

1Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea

2Department of Pharmacology, University of Ulsan College of Medicine, Seoul, South Korea

3Division of Food and Nutrition, Chonnam National University, Gwangju, South Korea

4Research Institute for Human Ecology, Chonnam National University, Gwangju, South Korea

5Department of Food Science and Nutrition, The University of Suwon, Kyunggi-do, South Korea

6Department of Medical Nutrition, Kyung Hee University, Yongin-si, Kyunggi-do, South Korea

7Marine Biotechnology Research Center, Jellanam-do, South Korea

Pub. Date: August 29, 2016

Cite this paper:
Hee-Bum Kang, Ji-Hye Song, Yanghee You, Jeongjin Park, Sungmin Kwak, Yoo-Hyun Lee, Jeongmin Lee, Su-il Kim, Kyung-Chul Choi and Woojin Jun. Porphyra tenera Extracts Have Immune Stimulation Activity via Increasing Cytokines in Mouse Primary Splenocytes and RAW264.7 Macrophages. Journal of Food and Nutrition Research. 2016; 4(9):558-565. doi: 10.12691/jfnr-4-9-1

Abstract

Porphyra tenera has long been consumed as food in Korea and Asia. The effects of Porphyra tenera extracts on the immune system are largely unknown. Therefore, this study investigated the immune-stimulating effects of ethanol and water extracts of P. tenera. The immunomodulatory potential of P. tenera was evaluated by determining its effect on cell viability and cytokine expression of mouse RAW264.7 cells and splenocytes. We investigated the effect of 10% ethanol extracts of laver (P. tenera) on the RAW264.7 cells. Production of nitric oxide (NO) and cytokines (interleukin [IL]-1β, IL-2, and IL-4, inducible NO synthase, and interferon-γ) in RAW264.7 macrophages was slightly higher after treatment with P. tenera extracts. Ethanol extracts upregulated and enhanced the functions of macrophages, such as NO and cytokines (IL-1β, IL-2, and IL-4, inducible NO synthase, and interferon-γ) production. In addition, cytokine concentrations were significantly increased in cells treated with different doses of P. tenera ethanol extracts compared to the control group. Overall, the results demonstrated that P. tenera extracts enhanced cytokine secretion in mouse splenocytes and macrophages. From these findings, it can be concluded that P. tenera possess a natural compound with immune-stimulatory activity. P. tenera extract is a good immunostimulant from natural compounds.

Keywords:
porphyra tenera immune stimulation RAW264.7 cells cytokines splenocytes

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Alexander RB, Ponniah S, Hasday J, Hebel JR: Elevated levels of proinflammatory cytokines in the semen of patients with chronic prostatitis/chronic pelvic pain syndrome. Urology. 1998; 52(5): 744-749.
 
[2]  Bogdan C: Nitric oxide and the immune response. Nature immunology. 2001; 2(10): 907-916.
 
[3]  Chaudhuri D, Suriano R, Mittelman A, Tiwari RK: Targeting the immune system in cancer. Current pharmaceutical biotechnology. 2009; 10(2): 166-184.
 
[4]  Jeremy JY, Rowe D, Emsley AM, Newby AC: Nitric oxide and the proliferation of vascular smooth muscle cells. Cardiovascular research. 1999; 43(3): 580-594.
 
[5]  Kim HJ, Chang EJ, Bae SJ, et al.: Cytotoxic and antimutagenic stilbenes from seeds of Paeonia lactiflora. Archives of pharmacal research. 2002; 25(3): 293-299.
 
[6]  Klimp AH, de Vries EG, Scherphof GL, Daemen T: A potential role of macrophage activation in the treatment of cancer. Critical reviews in oncology/hematology. 2002; 44(2): 143-161.
 
[7]  Lee E, Jeong KW, Shin A, et al.: Binding model for eriodictyol to Jun-N terminal kinase and its anti-inflammatory signaling pathway. BMB reports. 2013; 46(12): 594-599.
 
[8]  Lee JS, Hong EK: Immunostimulating activity of the polysaccharides isolated from Cordyceps militaris. International immunopharmacology. 2011; 11(9): 1226-1233.
 
[9]  Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH: Screening of medicinal plant extracts for antioxidant activity. Life sciences. 2003; 73(2): 167-179.
 
[10]  Lee WH, Gam CO, Ku SK, Choi SH: Single oral dose toxicity test of platycodin d, a saponin from platycodin radix in mice. Toxicological research. 2011; 27(4): 217-224.
 
[11]  Medzhitov R, Janeway C, Jr.: Innate immune recognition: mechanisms and pathways. Immunological reviews. 2000; 173: 89-97.
 
[12]  Na YS, Kim WJ, Kim SM, et al.: Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens. International immunopharmacology. 2010; 10(3): 364-370.
 
[13]  Senthil Kumar KJ, Wang SY: Lucidone inhibits iNOS and COX-2 expression in LPS-induced RAW 264.7 murine macrophage cells via NF-kappaB and MAPKs signaling pathways. Planta medica. 2009; 75(5): 494-500.
 
[14]  Turpaev K, Bouton C, Drapier JC: Nitric oxide-derived nitrosating species and gene expression in human monocytic cells. Biochemistry. 2004; 43(33): 10844-10850.
 
[15]  Umansky V, Hehner SP, Dumont A, et al.: Co-stimulatory effect of nitric oxide on endothelial NF-kappaB implies a physiological self-amplifying mechanism. European journal of immunology. 1998; 28(8): 2276-2282.
 
[16]  Vassalli P: The pathophysiology of tumor necrosis factors. Annual review of immunology. 1992; 10: 411-452.
 
[17]  Wink DA, Hines HB, Cheng RY, et al.: Nitric oxide and redox mechanisms in the immune response. Journal of leukocyte biology. 2011; 89(6): 873-891.
 
[18]  Yamamura Y, Azuma I: Immunostimulation in cancer patients. Advances in experimental medicine and biology. 1983; 166: 1-13.
 
[19]  Yu Q, Nie SP, Li WJ, et al.: Macrophage immunomodulatory activity of a purified polysaccharide isolated from Ganoderma atrum. Phytotherapy research : PTR. 2013; 27(2): 186-191.
 
[20]  Lull C, Harry J. W, Huub F. J. Savelkoul Antiinflammatory and Immunomodulating Properties of Fungal Metabolites. Mediators Inflamm. 2005 June 9; (2): 63-80.
 
[21]  Hwang ES, Ki KN, Chung HY. Proximate composition, amino Acid, mineral, and heavy metal content of dried laver. Prev Nutr Food Sci. 2013 Jun; 18(2): 139-44.
 
[22]  Hafting JT, Craigie JS, Stengel DB, Loureiro RR, Buschmann AH, Yarish C, Edwards MD, Critchley AT. Prospects and challenges for industrial production of seaweed bioactives.J Phycol. 2015 Oct; 51(5): 821-37.
 
[23]  Kitajima H, Komizu Y, Ichihara H, Goto K, Doi T, Inano T, Ueoka R. Immunostimulatory Effects of Extract from Nori (Porphyra yezoensis) in Vitro and in Vivo. KAGAKU KOGAKU RONBUNSHU, 2013. Vol. 39, No.4, 359-362,
 
[24]  Park SY, Kim HB, Kim JH, Lee JM, Kim SR, Shin HS, Yi TH. Immunostimulatory effect of fermented Red Ginseng in the Mouse Model. Prev Nutr Food Sci. 2014 Jan; 19(1): 10-8.
 
[25]  Hamed I, Özogul F, Özogul Y,Regenstein J. M. Marine Bioactive Compounds and Their Health Benefits: A Review. Comprehensive Reviews in Food Science and Food Safety. 2015, July Vol 14, Issue 4, pages 446-465.
 
[26]  Wojdasiewicz P, Poniatowski ŁA, Szukiewicz D. The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis.Mediators Inflamm. 2014; 2014: 561459.
 
[27]  Souza KL, Gurgul-Convey E, Elsner M, Lenzen S.Interaction between pro-inflammatory and anti-inflammatory cytokines in insulin-producing cells. J Endocrinol. 2008 Apr; 197(1): 139-50.
 
[28]  Sharifuddin Y, Chin YX, Lim PE, Phang SM. Potential Bioactive Compounds from Seaweed for Diabetes Management.Mar Drugs. 2015 Aug 21; 13(8): 5447-91.