Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2016, 4(7), 422-428
DOI: 10.12691/jfnr-4-7-2
Open AccessArticle

Biological Activity of Extracellular and Intracellular Polysaccharides from Pleurotus tuber-regium Hybrid and Mutant Strains

Comfort Olukemi Bamigboye1, 2, , Julius Kola Oloke1 and Joanna Felicity Dames2

1Department of Microbiology and Biotechnology, P.O.Box 94, Rhodes University, Grahamstown, Easterncape, South Africa

2Department of Pure and Applied Biology (Microbiology Unit), Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria

Pub. Date: July 14, 2016

Cite this paper:
Comfort Olukemi Bamigboye, Julius Kola Oloke and Joanna Felicity Dames. Biological Activity of Extracellular and Intracellular Polysaccharides from Pleurotus tuber-regium Hybrid and Mutant Strains. Journal of Food and Nutrition Research. 2016; 4(7):422-428. doi: 10.12691/jfnr-4-7-2


Pleurotus tuber-regium (Fr.) Singer (1951) is a unique sclerotium-forming edible and medicinal mushroom. Interestingly, both the sclerotium and mushroom are edible and are often used for curing various ailments. Previous studies have focused on the antimicrobial and antioxidant activity of the extracellular polysaccharide (EPS) from wild P. tuber-regium. There has been no report on the intracellular polysaccharide (IPS) of the wild mycelia, likewise there is very meager information on the improvement of the perceived potentials of P. tuber-regium. This research study analysed the EPS and IPS fractions of P. tuber-regium hybrid and mutant strains. The antimicrobial potential of the IPS and EPS fractions, their scavenging activity on 1, 1-diphenyl–2picryhydrazyl (DPPH) and Hydroxyl radicals were also determined. Both IPS and EPS fractions of P. tuber-regium hybrids and mutants showed increased DPPH and hydroxyl scavenging activity over the wild P. tuber-regium with an EC50 mostly < 1 mg/ml. The antimicrobial activity of the IPS from a mutant strain had an IC50 of 15.6 mg/ml compared to the wild type (18.75 mg /ml). This study showed that selected mutant and hybrids of P. tuber-regium had increased radical scavenging activity indicating potentially increased biological activity that could offer increased benefit as a neutraceutical.

antioxidant antimicrobial Pleurotus species sclerotium medicinal mushrooms Lentinus tuber-regium neutraceutical

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