Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(6), 361-368
DOI: 10.12691/jfnr-4-6-4
Open AccessArticle

Biological Activities of Fructooligosaccharides Produced by Aspergillus aculeatus in Mice Fed a High-fat Diet and Caco-2 Cell

Ok-Kyung Kim1, 2, Jeongjin Park1, 2, Ho-Geun Yoon3, Yanghee You1, 2, Yi Seul Seo4, Jae Hwan Kim4, Kyung-Chul Choi5, Yoo-Hyun Lee6, Jeongmin Lee7 and Woojin Jun1, 2,

1Division of Food and Nutritional Science, Chonnam National University, Gwangju61186, Republic of Korea

2Research Institute for Human Ecology, Chonnam National University, Gwangju61186, Republic of Korea

3Department of Biochemistry and Molecular Biology, Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul 03722, Republic of Korea

4Neo Cremar Co., Sungnam, 13229, Republic of Korea

5Department of Biomedical Sciences, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea

6Department of Food and Nutrition, University of Suwon, Suwon 18323, Republic of Korea

7Department of Medical Nutrition, Kyung Hee University, Yongin 17104, Republic of Korea

Pub. Date: July 05, 2016

Cite this paper:
Ok-Kyung Kim, Jeongjin Park, Ho-Geun Yoon, Yanghee You, Yi Seul Seo, Jae Hwan Kim, Kyung-Chul Choi, Yoo-Hyun Lee, Jeongmin Lee and Woojin Jun. Biological Activities of Fructooligosaccharides Produced by Aspergillus aculeatus in Mice Fed a High-fat Diet and Caco-2 Cell. Journal of Food and Nutrition Research. 2016; 4(6):361-368. doi: 10.12691/jfnr-4-6-4


We investigated the effects of the dietary supplement of fructooligosaccharides (FOS) on intestinal bacteria, blood cholesterol and mineral absorption in the Balb/c mice fed a high-fat diet. Mice were divided into four groups: Normal control (Balb/c mice fed normal diet), High fat control (Balb/c mice fed 60% fat diet), HF-FOS 5% (Balb/c mice fed 60% fat and 5% FOS diet), and HF-FOS 10% (Balb/c mice fed 60% fat and 10% FOS diet). We used an in vitro assay to evaluate whether the direct effects of FOS on calcium and magnesium transport improve absorption in the differentiated Caco-2 cell. The dietary supplementation with FOS resulted in a significant increase in Lactobacillus and Bifidobacterium in the feces and large intestine compared with the high fat control group. We found that dietary supplementation with FOS suppressed weight gain, hypercholesterolemia and hypertriglyceridemia induction by the high-fat diet although food consumption by the HF-FOS 5% and HF-FOS 10% groups was higher compared to the high fat control. Furthermore, the blood concentrations of calcium and magnesium that were lowered by the high fat diet were significantly increased in the HF-FOS 5% and HF-FOS 10% groups even though the absorption of calcium and magnesium following FOS treatment was low in the in vitro assay. Overall, these results suggest that dietary supplementation with FOS can help prevent a wide range of ailments induced by chronic high fat diet intake.

fructooligosaccharides intestinal bacteria high fat diet cholesterol mineral absorption

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